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The map label for this gene is proS [H]

Identifier: 189182913

GI number: 189182913

Start: 10343

End: 11644

Strand: Direct

Name: proS [H]

Synonym: OTT_0006

Alternate gene names: 189182913

Gene position: 10343-11644 (Clockwise)

Preceding gene: 189182912

Following gene: 189182915

Centisome position: 0.51

GC content: 31.18

Gene sequence:

>1302_bases
ATGCGCTTATCACAATATTTTTTACCACTACTTAAAGAAGATCCAGCTGGAGCTGAGATTATATCTCATAAACTAATGTT
GCGTGCAGGTATGATTAAGCAAGCATGTGCTGGAATTTATAACTGGTTACCATTAGGGATTAAAGTTTTACAGAACATTC
AGGATATTGTTAGAAAAAATATGAATGCAGCTCAATGCATTGAGCTGCTAATGCCATGCATTCAACCTGCCAGCTTATGG
AAAGAATCGTCTCGATATAATGCTTATGGAAAAGAAATGCTTAAAATCAAGGATAGACATGACAATGATCTCCTATTTGG
ACCTACTAATGAAGAACTAATCACAGATATTTTTAGAAGCTCTATTCAGTCATATAAAGATTTACCAAAGAATTTTTATC
ACATTCAGTGGAAATTTAGAGATGAAATTAGACCAAGATTTGGTGTAATGCGAAGCAGAGAATTTATAATGAAAGATGCT
TACTCTTTCGATATAGATCAAGCTTCTGCTATACAAAGTTATAATTTAATGTATCTCACTTACTTGAAAATTTTTAAGGA
TTTAGGTCTTAAAGTTATTCCAGTGCAGGCTGATAATGGAGCTATTGGTGGTAAATTAAGTCATGAGTTTCATATCATTA
CTGCTACAGGTGATAGCAAAATATATTATGATAGCACTAAATTTAACCCTTTTGACTACAACCAAGATTTATCTTTGAAT
AAATTAGAGCAATTATACATAGTTACTGAAGACAAGCATAAAACTGATCAAAATCAGATTTCAAATGATACAATATCTAC
TATATCAGGAATAGAAGTTGGTCATATATTTTACTTCGGAACTAAATATAGTGAATTAATGAAGGCTTGTATTCAACTGC
AAAACGGAAAACTTACAGCTGTTGAAATGGGGTCATATGGCATTGGAATCTCTAGATTAATAGGTGCTATTATTGAAGCT
AATCACGATGAGTACGGCATAATATGGCCACAAAATGTTGCACCATTTAAAGTTTCTGTTATTAACTTACTACCTAATAA
TGATAAGTGCAAACAAACATCAAATCAGCTATATCAAATCTTAACTCAGCAAAAAACAGATGCATTATTAGATGATACTT
CAAGTTCTGCTGGCCATAAATTAGCTGCTCATGATTTAATAGGTATACCATATCAGATAATTATAGGCAAAAATTTTATT
GAAACTGCAACTATAGAATTTAAATATCGTCATAATCAGCAGCTTCAAAAATTAAGCATTGATGCTTTAATTAATCAATT
AACTGAAATATCTAAGCATTAA

Upstream 100 bases:

>100_bases
CTCTTAAAGTTAAATTATAGTAGATCAGAAATCATTTATACAGGATCTTCTGAATCTGCTAAAAAACTATTGCAACTATA
TAATTAAGAAAATCAAGTTA

Downstream 100 bases:

>100_bases
TATTATCTAACTAATTACTATAGTCATTTACAATGAAGTTTGCGTATAAAATATCTTGTTGGTAAATCTTATGATGCTAT
TGTCGCTTTTTTCTATGCTC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MRLSQYFLPLLKEDPAGAEIISHKLMLRAGMIKQACAGIYNWLPLGIKVLQNIQDIVRKNMNAAQCIELLMPCIQPASLW
KESSRYNAYGKEMLKIKDRHDNDLLFGPTNEELITDIFRSSIQSYKDLPKNFYHIQWKFRDEIRPRFGVMRSREFIMKDA
YSFDIDQASAIQSYNLMYLTYLKIFKDLGLKVIPVQADNGAIGGKLSHEFHIITATGDSKIYYDSTKFNPFDYNQDLSLN
KLEQLYIVTEDKHKTDQNQISNDTISTISGIEVGHIFYFGTKYSELMKACIQLQNGKLTAVEMGSYGIGISRLIGAIIEA
NHDEYGIIWPQNVAPFKVSVINLLPNNDKCKQTSNQLYQILTQQKTDALLDDTSSSAGHKLAAHDLIGIPYQIIIGKNFI
ETATIEFKYRHNQQLQKLSIDALINQLTEISKH

Sequences:

>Translated_433_residues
MRLSQYFLPLLKEDPAGAEIISHKLMLRAGMIKQACAGIYNWLPLGIKVLQNIQDIVRKNMNAAQCIELLMPCIQPASLW
KESSRYNAYGKEMLKIKDRHDNDLLFGPTNEELITDIFRSSIQSYKDLPKNFYHIQWKFRDEIRPRFGVMRSREFIMKDA
YSFDIDQASAIQSYNLMYLTYLKIFKDLGLKVIPVQADNGAIGGKLSHEFHIITATGDSKIYYDSTKFNPFDYNQDLSLN
KLEQLYIVTEDKHKTDQNQISNDTISTISGIEVGHIFYFGTKYSELMKACIQLQNGKLTAVEMGSYGIGISRLIGAIIEA
NHDEYGIIWPQNVAPFKVSVINLLPNNDKCKQTSNQLYQILTQQKTDALLDDTSSSAGHKLAAHDLIGIPYQIIIGKNFI
ETATIEFKYRHNQQLQKLSIDALINQLTEISKH
>Mature_433_residues
MRLSQYFLPLLKEDPAGAEIISHKLMLRAGMIKQACAGIYNWLPLGIKVLQNIQDIVRKNMNAAQCIELLMPCIQPASLW
KESSRYNAYGKEMLKIKDRHDNDLLFGPTNEELITDIFRSSIQSYKDLPKNFYHIQWKFRDEIRPRFGVMRSREFIMKDA
YSFDIDQASAIQSYNLMYLTYLKIFKDLGLKVIPVQADNGAIGGKLSHEFHIITATGDSKIYYDSTKFNPFDYNQDLSLN
KLEQLYIVTEDKHKTDQNQISNDTISTISGIEVGHIFYFGTKYSELMKACIQLQNGKLTAVEMGSYGIGISRLIGAIIEA
NHDEYGIIWPQNVAPFKVSVINLLPNNDKCKQTSNQLYQILTQQKTDALLDDTSSSAGHKLAAHDLIGIPYQIIIGKNFI
ETATIEFKYRHNQQLQKLSIDALINQLTEISKH

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=420, Percent_Identity=34.5238095238095, Blast_Score=251, Evalue=9e-67,
Organism=Escherichia coli, GI1786392, Length=239, Percent_Identity=53.1380753138075, Blast_Score=283, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI115532348, Length=423, Percent_Identity=30.7328605200946, Blast_Score=185, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6320931, Length=201, Percent_Identity=38.3084577114428, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24656200, Length=381, Percent_Identity=30.1837270341207, Blast_Score=208, Evalue=8e-54,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 49453; Mature: 49453

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSQYFLPLLKEDPAGAEIISHKLMLRAGMIKQACAGIYNWLPLGIKVLQNIQDIVRKN
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MNAAQCIELLMPCIQPASLWKESSRYNAYGKEMLKIKDRHDNDLLFGPTNEELITDIFRS
CCHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHEEECCCCCCEEECCCCHHHHHHHHHH
SIQSYKDLPKNFYHIQWKFRDEIRPRFGVMRSREFIMKDAYSFDIDQASAIQSYNLMYLT
HHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHH
YLKIFKDLGLKVIPVQADNGAIGGKLSHEFHIITATGDSKIYYDSTKFNPFDYNQDLSLN
HHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCEEEECCCCCCCCCCCCCCCCC
KLEQLYIVTEDKHKTDQNQISNDTISTISGIEVGHIFYFGTKYSELMKACIQLQNGKLTA
CEEEEEEEECCCCCCCHHHCCCCHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCCCEEE
VEMGSYGIGISRLIGAIIEANHDEYGIIWPQNVAPFKVSVINLLPNNDKCKQTSNQLYQI
EECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
LTQQKTDALLDDTSSSAGHKLAAHDLIGIPYQIIIGKNFIETATIEFKYRHNQQLQKLSI
HHHHHHHHHHCCCCCCCCCEEHHHHHCCCCEEEEECCCCCEEEEEEEEECCCHHHHHHHH
DALINQLTEISKH
HHHHHHHHHHHCC
>Mature Secondary Structure
MRLSQYFLPLLKEDPAGAEIISHKLMLRAGMIKQACAGIYNWLPLGIKVLQNIQDIVRKN
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MNAAQCIELLMPCIQPASLWKESSRYNAYGKEMLKIKDRHDNDLLFGPTNEELITDIFRS
CCHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHEEECCCCCCEEECCCCHHHHHHHHHH
SIQSYKDLPKNFYHIQWKFRDEIRPRFGVMRSREFIMKDAYSFDIDQASAIQSYNLMYLT
HHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHH
YLKIFKDLGLKVIPVQADNGAIGGKLSHEFHIITATGDSKIYYDSTKFNPFDYNQDLSLN
HHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCEEEECCCCCCCCCCCCCCCCC
KLEQLYIVTEDKHKTDQNQISNDTISTISGIEVGHIFYFGTKYSELMKACIQLQNGKLTA
CEEEEEEEECCCCCCCHHHCCCCHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCCCEEE
VEMGSYGIGISRLIGAIIEANHDEYGIIWPQNVAPFKVSVINLLPNNDKCKQTSNQLYQI
EECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHH
LTQQKTDALLDDTSSSAGHKLAAHDLIGIPYQIIIGKNFIETATIEFKYRHNQQLQKLSI
HHHHHHHHHHCCCCCCCCCEEHHHHHCCCCEEEEECCCCCEEEEEEEEECCCHHHHHHHH
DALINQLTEISKH
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA