Click here to switch to the map view.

The map label for this gene is pheS

Identifier: 189026196

GI number: 189026196

Start: 1057782

End: 1059443

Strand: Direct

Name: pheS

Synonym: TPASS_0973

Alternate gene names: 189026196

Gene position: 1057782-1059443 (Clockwise)

Preceding gene: 189026181

Following gene: 189026199

Centisome position: 92.83

GC content: 57.82

Gene sequence:

>1662_bases
ATGATGCCGCGCATGACAGGTAAAGCCGATCTGAACACGCTTGTGCACAAGCTGCATCCCCTTGAGATCAAAGTCCTCAA
GAACTGCGCAATGGATGAGATCCTTTCGACTTCGTTGCTCATCTCCCGGCTAGGCTTTAAGGAAGGACACGCAAACCAAG
CGTTTTCCTGGCTTCGGGCAAAGCGAATCATCGAAGAGCACCAGCGGGAACAGATGCGGTCCTTTGAACTCACCCCCTGC
GGCTATGCTGCCGCTAGCGACGGTACTGCAGAGGAGCGTATGCTCACCTTCTTGTCTTCCCCCCCTTCCCTTACCGCTAT
TGCAGATGCAGCCGAGCACCTACACCCTCGCCCCCCACTTTGCAACGGGCTCAGTCTCCCTGAGCTTGCCCATGCGCTCA
CGCTCGCACCAAAGGATGTAGGTTCAGCGTTCGGTATCCTAGCGCAGGAGGGTATCCTCCGTATGGACGGTGAAAAACGC
ATACACATCGTTTCACCGCACGTAAGTGATCGCATGTCCCTCACGCGCACCTTGCTTCAGCGCGCCGCTGCACGCGTCGC
TTCCCCGTCAGAGGCCTCGGACACTCCTCCAGGCACCCTCTTTGAATCTGAGCTCTCTGATGATGAGCGACGTGTAATGG
AACGCATAGCAAAAAAGCGGGGCGCAAGCGACAGCCTCTTCAAAGTCAGTGTACGCGAGAGGGTAACGTTCACCTTTACC
CCCACCGCCCGTGCCGTGCAGGAGGCGCTGCATACCGCAGGGCTTACCGGAAACGAGATAGGTGCACTCACCGTCGAGTG
CCTAAAAAGTGGCGCGTGGAAGACACAACATCTACGCCGCTACAACGTTCACATTCCCCCTGCCCGCATCATCCCCGGCC
GATCTAATGCCTACGCAGATTTCCTCGAACACATCAAAGATCGTCTGGTGGCACTTGGCTTTCAGGAATTTGACGGGCCA
CTGGTGGAAACAGACTTCTGGAACGCGGACGCGCTGTTCATGCCACAGTTCCATCCTGCACGGGACATTCATGATGTGTA
TTACCTCAAACACCCAACGCACGCACCTACAATCCCAGAGCCGTTCCTCTCCCGCGTTGCGGCGACGCATGAACGCGGAG
CAGATAGCGGGAGCCTCGGCTGGCGCTACTCGTTCGATCGGGATTTTACCCGCCGACTGCTATTACGCAGCCAAGGAACA
GCACTTTCAGCGCGCCACCTGCCAACCGCCCACATACCGGGTAAGTATTTTGGGATCGCACGCTGCTTCCGTCACGACCA
AGTGGATGCGACGCACCTTGCAGATTTCTACCAAACAGAAGGAATCGTACTGGGTACGGATGTGAACGTCTGTACCCTTC
TAGGCATGCTCAAAATCCTCGCAACAGAAATCGCCGGTGCACAGGAGGTACGCTACGTGGGCGGGTACTTTCCGTTCACG
GAGCCCTCTATCGAGCTACACGCACTCCACCCCGCACTCGGCTGGTTCGAACTGGGAGGAGCAGGCCTCCTGCGCCCAGA
AGTGACCGACCCGCTCGGCGTGCACGTCCCCGTCATGGCCTGGGGACTAGGAGTAGACCGCATGGCGCTCCTGGCGCTCG
GTATCTCAGACGTGCGAGAACTGTTCAGCCCAGACATCGAGAGCGTCCGCCTGAGGGTATAA

Upstream 100 bases:

>100_bases
AGGATCGTTCCATCTATTTCTCCACACTACGGCAGATGTACCCTGATGCAGAACGAAAAATTGAGAAACAGCCTACATCT
AAACGGCGCGAAAATACGTT

Downstream 100 bases:

>100_bases
ACACAACAGCCTGTACGCCCTCACCCGGACCTAAAAAGCCTGCCTACAGGCTTATAAATTCTGCTCCTCCAGGAAGCGCT
CCACCACGTGGGACAGCGCC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MMPRMTGKADLNTLVHKLHPLEIKVLKNCAMDEILSTSLLISRLGFKEGHANQAFSWLRAKRIIEEHQREQMRSFELTPC
GYAAASDGTAEERMLTFLSSPPSLTAIADAAEHLHPRPPLCNGLSLPELAHALTLAPKDVGSAFGILAQEGILRMDGEKR
IHIVSPHVSDRMSLTRTLLQRAAARVASPSEASDTPPGTLFESELSDDERRVMERIAKKRGASDSLFKVSVRERVTFTFT
PTARAVQEALHTAGLTGNEIGALTVECLKSGAWKTQHLRRYNVHIPPARIIPGRSNAYADFLEHIKDRLVALGFQEFDGP
LVETDFWNADALFMPQFHPARDIHDVYYLKHPTHAPTIPEPFLSRVAATHERGADSGSLGWRYSFDRDFTRRLLLRSQGT
ALSARHLPTAHIPGKYFGIARCFRHDQVDATHLADFYQTEGIVLGTDVNVCTLLGMLKILATEIAGAQEVRYVGGYFPFT
EPSIELHALHPALGWFELGGAGLLRPEVTDPLGVHVPVMAWGLGVDRMALLALGISDVRELFSPDIESVRLRV

Sequences:

>Translated_553_residues
MMPRMTGKADLNTLVHKLHPLEIKVLKNCAMDEILSTSLLISRLGFKEGHANQAFSWLRAKRIIEEHQREQMRSFELTPC
GYAAASDGTAEERMLTFLSSPPSLTAIADAAEHLHPRPPLCNGLSLPELAHALTLAPKDVGSAFGILAQEGILRMDGEKR
IHIVSPHVSDRMSLTRTLLQRAAARVASPSEASDTPPGTLFESELSDDERRVMERIAKKRGASDSLFKVSVRERVTFTFT
PTARAVQEALHTAGLTGNEIGALTVECLKSGAWKTQHLRRYNVHIPPARIIPGRSNAYADFLEHIKDRLVALGFQEFDGP
LVETDFWNADALFMPQFHPARDIHDVYYLKHPTHAPTIPEPFLSRVAATHERGADSGSLGWRYSFDRDFTRRLLLRSQGT
ALSARHLPTAHIPGKYFGIARCFRHDQVDATHLADFYQTEGIVLGTDVNVCTLLGMLKILATEIAGAQEVRYVGGYFPFT
EPSIELHALHPALGWFELGGAGLLRPEVTDPLGVHVPVMAWGLGVDRMALLALGISDVRELFSPDIESVRLRV
>Mature_553_residues
MMPRMTGKADLNTLVHKLHPLEIKVLKNCAMDEILSTSLLISRLGFKEGHANQAFSWLRAKRIIEEHQREQMRSFELTPC
GYAAASDGTAEERMLTFLSSPPSLTAIADAAEHLHPRPPLCNGLSLPELAHALTLAPKDVGSAFGILAQEGILRMDGEKR
IHIVSPHVSDRMSLTRTLLQRAAARVASPSEASDTPPGTLFESELSDDERRVMERIAKKRGASDSLFKVSVRERVTFTFT
PTARAVQEALHTAGLTGNEIGALTVECLKSGAWKTQHLRRYNVHIPPARIIPGRSNAYADFLEHIKDRLVALGFQEFDGP
LVETDFWNADALFMPQFHPARDIHDVYYLKHPTHAPTIPEPFLSRVAATHERGADSGSLGWRYSFDRDFTRRLLLRSQGT
ALSARHLPTAHIPGKYFGIARCFRHDQVDATHLADFYQTEGIVLGTDVNVCTLLGMLKILATEIAGAQEVRYVGGYFPFT
EPSIELHALHPALGWFELGGAGLLRPEVTDPLGVHVPVMAWGLGVDRMALLALGISDVRELFSPDIESVRLRV

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=298, Percent_Identity=37.5838926174497, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1788007, Length=273, Percent_Identity=27.8388278388278, Blast_Score=113, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17508957, Length=293, Percent_Identity=34.8122866894198, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI32563657, Length=295, Percent_Identity=34.9152542372881, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6321087, Length=289, Percent_Identity=33.9100346020761, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI18858079, Length=306, Percent_Identity=34.9673202614379, Blast_Score=179, Evalue=4e-45,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_TREPA (O83938)

Other databases:

- EMBL:   AE000520
- PIR:   C71257
- RefSeq:   NP_219410.1
- ProteinModelPortal:   O83938
- SMR:   O83938
- IntAct:   O83938
- GeneID:   2611417
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0973
- NMPDR:   fig|243276.1.peg.968
- TIGR:   TP_0973
- HOGENOM:   HBG497668
- OMA:   WFELGGS
- ProtClustDB:   PRK04172
- BioCyc:   TPAL243276:TP_0973-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00282
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF01409 tRNA-synt_2d

EC number: =6.1.1.20

Molecular weight: Translated: 61234; Mature: 61234

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPRMTGKADLNTLVHKLHPLEIKVLKNCAMDEILSTSLLISRLGFKEGHANQAFSWLRA
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
KRIIEEHQREQMRSFELTPCGYAAASDGTAEERMLTFLSSPPSLTAIADAAEHLHPRPPL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
CNGLSLPELAHALTLAPKDVGSAFGILAQEGILRMDGEKRIHIVSPHVSDRMSLTRTLLQ
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCEEEEECCCCHHHHHHHHHHHH
RAAARVASPSEASDTPPGTLFESELSDDERRVMERIAKKRGASDSLFKVSVRERVTFTFT
HHHHHHCCCCCCCCCCCCCEEHHHCCCHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEC
PTARAVQEALHTAGLTGNEIGALTVECLKSGAWKTQHLRRYNVHIPPARIIPGRSNAYAD
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHEECCCCCCCEECCCCCCHHHH
FLEHIKDRLVALGFQEFDGPLVETDFWNADALFMPQFHPARDIHDVYYLKHPTHAPTIPE
HHHHHHHHHHHHCHHHCCCCEEECCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCH
PFLSRVAATHERGADSGSLGWRYSFDRDFTRRLLLRSQGTALSARHLPTAHIPGKYFGIA
HHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHH
RCFRHDQVDATHLADFYQTEGIVLGTDVNVCTLLGMLKILATEIAGAQEVRYVGGYFPFT
HHHHCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
EPSIELHALHPALGWFELGGAGLLRPEVTDPLGVHVPVMAWGLGVDRMALLALGISDVRE
CCCEEEEEECCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHCHHHHHH
LFSPDIESVRLRV
HHCCCHHHEEECC
>Mature Secondary Structure
MMPRMTGKADLNTLVHKLHPLEIKVLKNCAMDEILSTSLLISRLGFKEGHANQAFSWLRA
CCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
KRIIEEHQREQMRSFELTPCGYAAASDGTAEERMLTFLSSPPSLTAIADAAEHLHPRPPL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCC
CNGLSLPELAHALTLAPKDVGSAFGILAQEGILRMDGEKRIHIVSPHVSDRMSLTRTLLQ
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCEEEEECCCCHHHHHHHHHHHH
RAAARVASPSEASDTPPGTLFESELSDDERRVMERIAKKRGASDSLFKVSVRERVTFTFT
HHHHHHCCCCCCCCCCCCCEEHHHCCCHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEC
PTARAVQEALHTAGLTGNEIGALTVECLKSGAWKTQHLRRYNVHIPPARIIPGRSNAYAD
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHEECCCCCCCEECCCCCCHHHH
FLEHIKDRLVALGFQEFDGPLVETDFWNADALFMPQFHPARDIHDVYYLKHPTHAPTIPE
HHHHHHHHHHHHCHHHCCCCEEECCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCH
PFLSRVAATHERGADSGSLGWRYSFDRDFTRRLLLRSQGTALSARHLPTAHIPGKYFGIA
HHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHH
RCFRHDQVDATHLADFYQTEGIVLGTDVNVCTLLGMLKILATEIAGAQEVRYVGGYFPFT
HHHHCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
EPSIELHALHPALGWFELGGAGLLRPEVTDPLGVHVPVMAWGLGVDRMALLALGISDVRE
CCCEEEEEECCCCCHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHCHHHHHH
LFSPDIESVRLRV
HHCCCHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876