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The map label for this gene is trmD

Identifier: 189026131

GI number: 189026131

Start: 989336

End: 990088

Strand: Direct

Name: trmD

Synonym: TPASS_0908

Alternate gene names: 189026131

Gene position: 989336-990088 (Clockwise)

Preceding gene: 189026130

Following gene: 189026132

Centisome position: 86.83

GC content: 55.64

Gene sequence:

>753_bases
ATGAACATTGACGTACTGACTCTCTTTCCCGCCATCCCGCGGGTTTATTTCTCCACCTCAATAATGGCGCGCGCCGTGAG
CGACGGGATCATTCACTACAATATTGTCAACATCCGTGACTTTGCGCACGACAAACACAAACACTGTGACGCCCCGCCCT
ACGGGGGCGGACCTGGCATGCTCATGCGCTCAGAACCGTTAGGGAAGGCCCTGGATTCAGTAGACGCTCCCAAAAAGCGC
GTCGTGTACGTCACTCCCTCTGGAAAGCTCTTTGAGCAAGGCTATGCGCGCTCACTCGCACAGGAACGTGCCCTCGTGCT
CATCTGCGGCAGATACGAGGGAATTGACCAACGTATCATAGACGAGTATGTCGACGACGAAGTTTGCATCGGGAACTACG
TTCTCTCCTCAGGAGAGATCGCCGCGCTCGTGCTCATCGATGCGGTGAGCCGCTGTGTTAGCGGTGTCATCCGCCATGAA
TCTCTCGAGGAGGAAAGTTTTGTAAACAGTCTCGTGGAGTACCCGCAGTACACCAGGCCGCGCTGTTTTCACAACCGGGA
CGTTCCCCCTGTACTTCTCTCCGGTCACCACGCGCATATCCGCACGTGGCGGCTTGCGCGCCAGATCGAAAAAACACGAA
GGAATAGACCTGACCTCCTCAGCGCTGCGCGTGCGTCTGCAGCCTGGACTCAAGAGGCTGAGTCCCTTCTTAAGGAGTTA
GACTATGAGTTGCCACCTCATCCAACAAATTGA

Upstream 100 bases:

>100_bases
CACATGGTGGCGTTTGCTATGTGCCTTTTCACCGGACCTTCATTGGGGACGTGGACGTTGGTCGAAAAAAAATCGAGTTG
TTGCAGCGGTGGATTCTCGA

Downstream 100 bases:

>100_bases
AAACCAGCAGCGAAAGGAACCAGCTGAAACGTTCCGCGTCGGGGACACCGTACGTGTGCATTTCAAAATAGTGGAGGGAA
AAACAGAGCGCATCCAAGCT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MNIDVLTLFPAIPRVYFSTSIMARAVSDGIIHYNIVNIRDFAHDKHKHCDAPPYGGGPGMLMRSEPLGKALDSVDAPKKR
VVYVTPSGKLFEQGYARSLAQERALVLICGRYEGIDQRIIDEYVDDEVCIGNYVLSSGEIAALVLIDAVSRCVSGVIRHE
SLEEESFVNSLVEYPQYTRPRCFHNRDVPPVLLSGHHAHIRTWRLARQIEKTRRNRPDLLSAARASAAWTQEAESLLKEL
DYELPPHPTN

Sequences:

>Translated_250_residues
MNIDVLTLFPAIPRVYFSTSIMARAVSDGIIHYNIVNIRDFAHDKHKHCDAPPYGGGPGMLMRSEPLGKALDSVDAPKKR
VVYVTPSGKLFEQGYARSLAQERALVLICGRYEGIDQRIIDEYVDDEVCIGNYVLSSGEIAALVLIDAVSRCVSGVIRHE
SLEEESFVNSLVEYPQYTRPRCFHNRDVPPVLLSGHHAHIRTWRLARQIEKTRRNRPDLLSAARASAAWTQEAESLLKEL
DYELPPHPTN
>Mature_250_residues
MNIDVLTLFPAIPRVYFSTSIMARAVSDGIIHYNIVNIRDFAHDKHKHCDAPPYGGGPGMLMRSEPLGKALDSVDAPKKR
VVYVTPSGKLFEQGYARSLAQERALVLICGRYEGIDQRIIDEYVDDEVCIGNYVLSSGEIAALVLIDAVSRCVSGVIRHE
SLEEESFVNSLVEYPQYTRPRCFHNRDVPPVLLSGHHAHIRTWRLARQIEKTRRNRPDLLSAARASAAWTQEAESLLKEL
DYELPPHPTN

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=247, Percent_Identity=43.3198380566802, Blast_Score=206, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_TREPA (O83878)

Other databases:

- EMBL:   AE000520
- PIR:   A71268
- RefSeq:   NP_219343.1
- ProteinModelPortal:   O83878
- SMR:   O83878
- GeneID:   2611237
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0908
- NMPDR:   fig|243276.1.peg.902
- TIGR:   TP_0908
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   TPAL243276:TP_0908-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28119; Mature: 28119

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDVLTLFPAIPRVYFSTSIMARAVSDGIIHYNIVNIRDFAHDKHKHCDAPPYGGGPGM
CCCEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCC
LMRSEPLGKALDSVDAPKKRVVYVTPSGKLFEQGYARSLAQERALVLICGRYEGIDQRII
EEECCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHH
DEYVDDEVCIGNYVLSSGEIAALVLIDAVSRCVSGVIRHESLEEESFVNSLVEYPQYTRP
HHHCCCCEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
RCFHNRDVPPVLLSGHHAHIRTWRLARQIEKTRRNRPDLLSAARASAAWTQEAESLLKEL
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
DYELPPHPTN
CCCCCCCCCC
>Mature Secondary Structure
MNIDVLTLFPAIPRVYFSTSIMARAVSDGIIHYNIVNIRDFAHDKHKHCDAPPYGGGPGM
CCCEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHCCCCCCCCCCCCCC
LMRSEPLGKALDSVDAPKKRVVYVTPSGKLFEQGYARSLAQERALVLICGRYEGIDQRII
EEECCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHH
DEYVDDEVCIGNYVLSSGEIAALVLIDAVSRCVSGVIRHESLEEESFVNSLVEYPQYTRP
HHHCCCCEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
RCFHNRDVPPVLLSGHHAHIRTWRLARQIEKTRRNRPDLLSAARASAAWTQEAESLLKEL
CCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
DYELPPHPTN
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876