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The map label for this gene is nth

Identifier: 189026000

GI number: 189026000

Start: 844340

End: 844975

Strand: Direct

Name: nth

Synonym: TPASS_0775

Alternate gene names: 189026000

Gene position: 844340-844975 (Clockwise)

Preceding gene: 189025999

Following gene: 189026009

Centisome position: 74.1

GC content: 55.19

Gene sequence:

>636_bases
ATGCGTCTATTAGACAGCAAGGGGGTACACGCCGTTTTTGAGCAGCTGCACGCAGCAAACCCGCAACCACAAGGAGAACT
TCACTGGCGCAATACTTTTACGCTCCTGGTGGCAGTACTGCTTTCTGCGCAGGCAACAGACAAAAGCGTCAACAAGGCGA
CCGCTGCTCTGTTTGATGTGGCAGACACCCCCCAAGCAATGCTCGCGCTGGGGGAAGAACGGCTATGCTCCTACATACGG
ACGATTAATTTATACCCCACGAAAGCACGCCGAATCATTGCACTCAGTGCTGAACTCATCGAGCGCTTCGCTGCCCAAGT
ACCGTGCGACGCCCATGCCCTAGAGAGTCTACCCGGGGTCGGTCACAAGACGGCAAACGTAGTCCTGAACATGGGTTTTG
GCATACCCACAATCGCAGTTGATACGCATATCCTGCGGACAGCGCCACGCATCGGGCTTTCCTCTGGAAGAACCCCACGC
GCGGTTGAACGCGACTTGCTTGTGGTCACCCCGCGTGAGTTTCGCATGCATGCACATCACTGGATTTTACTCCACGGACG
CTACACCTGCACCGCACGAAGACCACGGTGCACGGAGTGCTGCCTAAGAGACCTATGCTGCAAAAACAACATATAA

Upstream 100 bases:

>100_bases
GTACTTCAGGAAACTCTCTGAATCCTGTGCGTTCATTTCCCTCCTTTCCACCCCATGACCTCTTGCACTTTTTAAACTAC
CTCTCTTTACTGGCACCCTT

Downstream 100 bases:

>100_bases
GAGAAAAATTGCTGGGCCGTACGTCCTGCGGCATTTTCAGGAATTTCTAAAAGAAGTTCGCTAACTAATTGACGCCTCAC
GCAAAAAAGAACGAGAAACC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MRLLDSKGVHAVFEQLHAANPQPQGELHWRNTFTLLVAVLLSAQATDKSVNKATAALFDVADTPQAMLALGEERLCSYIR
TINLYPTKARRIIALSAELIERFAAQVPCDAHALESLPGVGHKTANVVLNMGFGIPTIAVDTHILRTAPRIGLSSGRTPR
AVERDLLVVTPREFRMHAHHWILLHGRYTCTARRPRCTECCLRDLCCKNNI

Sequences:

>Translated_211_residues
MRLLDSKGVHAVFEQLHAANPQPQGELHWRNTFTLLVAVLLSAQATDKSVNKATAALFDVADTPQAMLALGEERLCSYIR
TINLYPTKARRIIALSAELIERFAAQVPCDAHALESLPGVGHKTANVVLNMGFGIPTIAVDTHILRTAPRIGLSSGRTPR
AVERDLLVVTPREFRMHAHHWILLHGRYTCTARRPRCTECCLRDLCCKNNI
>Mature_211_residues
MRLLDSKGVHAVFEQLHAANPQPQGELHWRNTFTLLVAVLLSAQATDKSVNKATAALFDVADTPQAMLALGEERLCSYIR
TINLYPTKARRIIALSAELIERFAAQVPCDAHALESLPGVGHKTANVVLNMGFGIPTIAVDTHILRTAPRIGLSSGRTPR
AVERDLLVVTPREFRMHAHHWILLHGRYTCTARRPRCTECCLRDLCCKNNI

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=27.683615819209, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1787920, Length=195, Percent_Identity=55.8974358974359, Blast_Score=219, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=29.7142857142857, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324530, Length=191, Percent_Identity=27.2251308900524, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=27.1186440677966, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END3_TREPA (O83754)

Other databases:

- EMBL:   AE000520
- PIR:   H71281
- RefSeq:   NP_219212.1
- ProteinModelPortal:   O83754
- SMR:   O83754
- GeneID:   2611450
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0775
- NMPDR:   fig|243276.1.peg.771
- TIGR:   TP_0775
- HOGENOM:   HBG464473
- OMA:   FGEPTIA
- ProtClustDB:   CLSK493747
- BioCyc:   TPAL243276:TP_0775-MONOMER
- BRENDA:   4.2.99.18
- GO:   GO:0005622
- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170
- Gene3D:   G3DSA:1.10.340.30
- Gene3D:   G3DSA:1.10.1670.10
- SMART:   SM00478
- SMART:   SM00525
- SMART:   SM00278
- TIGRFAMs:   TIGR01083

Pfam domain/function: PF00730 HhH-GPD; SSF48150 DNA_glycsylse

EC number: =4.2.99.18

Molecular weight: Translated: 23420; Mature: 23420

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00764 ENDONUCLEASE_III_1; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLDSKGVHAVFEQLHAANPQPQGELHWRNTFTLLVAVLLSAQATDKSVNKATAALFDV
CCCCCCCCHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
ADTPQAMLALGEERLCSYIRTINLYPTKARRIIALSAELIERFAAQVPCDAHALESLPGV
CCCHHHHHHHCHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHHCCCCHHHHHHCCCC
GHKTANVVLNMGFGIPTIAVDTHILRTAPRIGLSSGRTPRAVERDLLVVTPREFRMHAHH
CCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHCCCEEEECCHHHHHCCCE
WILLHGRYTCTARRPRCTECCLRDLCCKNNI
EEEEECCEEEECCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLLDSKGVHAVFEQLHAANPQPQGELHWRNTFTLLVAVLLSAQATDKSVNKATAALFDV
CCCCCCCCHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
ADTPQAMLALGEERLCSYIRTINLYPTKARRIIALSAELIERFAAQVPCDAHALESLPGV
CCCHHHHHHHCHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHHHHHCCCCHHHHHHCCCC
GHKTANVVLNMGFGIPTIAVDTHILRTAPRIGLSSGRTPRAVERDLLVVTPREFRMHAHH
CCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHCCCEEEECCHHHHHCCCE
WILLHGRYTCTARRPRCTECCLRDLCCKNNI
EEEEECCEEEECCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876