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The map label for this gene is potB [H]

Identifier: 189025878

GI number: 189025878

Start: 718302

End: 719201

Strand: Direct

Name: potB [H]

Synonym: TPASS_0653

Alternate gene names: 189025878

Gene position: 718302-719201 (Clockwise)

Preceding gene: 189025877

Following gene: 189025879

Centisome position: 63.04

GC content: 55.11

Gene sequence:

>900_bases
GTGAGCAGCCGGCTGCGCGTGGCGCGACGCCGTACCGTAGGCAGTTTAGGTTTTTGGTGCACGCTCCCCTTGTCTGCGTG
GCTTACAGTCTTCTTTCTGTGCCCCCTTACGGTGGTCGCTGTGTACAGCTTTTTGCAAAAGGGCCTGTACGGAGGCGTTG
TGTGGAAGTTTTCCATCCGGGCGTATGCGCAAGTCCTTACCTCCGGCTATGGCGCACTGCTGGCTCGCACCCTGCTTATC
AGTACAGGCGTCACCTGTTTGTGCGTGCTCCTGGCACTTCCTGCAGGCTACGCGCTCTCTCGCAGCCGGAGGCAAACGTT
CTTCCTGTTCCTAATTATCGTCCCCTTTTGGACCAATTCTCTCATCAGGATAAACGCCTGGATCACTATCCTGGGGAACC
AGGGGTTTTTGAACGAAGCACTCAAGTCGCTTGGTTTTATTACTCGAAGTCTTCCGCTCCTGTACCATAACGGGGCAGTC
ATTGTGGTGCTCACCTATATGTTCCTGCCCTACGCTATCTTTCCTATTTTCGCCGCTATCGATCGCTTCGATTTCTCCCT
CCTTGAATCTGCATGCGATCTAGGCTCTACCCCCACGGGTGCCATTATCCGCGTGTTGCTTCCCAACATCAAAACCGGCA
TTGCCACCGCCGTGTTCTTTACCTTCATTCCTGTCTTCGGTTCCTACACCGTCCCCCTCCTGGTGGGAGGAAAGGATTCC
TACCTTATCGGAAACGCAATTGTGGACCAGGTACGTATCGTGGGAAATTGGCCACTCGCTTCGGCCTTCGCGATGCTCGT
TACCCTCGCAGGGGGGGTAGGAGTACTCTGGATACTTGCAGAAAGCGGTGCCTGCAATACAACACCTCCCCCTTGCGGAG
GACGCCGCTCATATGCCTAA

Upstream 100 bases:

>100_bases
ACCAGAAGTATCTCGACACTGGCCCTGAGATTGCGTGGAAGGACACTGTTTACGTGTCCTGGTCTGCACACGACGGCTAC
GTGGTTGAGGACATCGAGTC

Downstream 100 bases:

>100_bases
TTGCCGTCTGATCCACACGCGCGCACGCACTCCCCTACGGTGTTCCTTCTCTGCTGTGCTGCTGGCGGCAGTTGTCTCGT
TCCTATTTCTGCCGCTTGCA

Product: spermidine/putrescine ABC transporter, permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSSRLRVARRRTVGSLGFWCTLPLSAWLTVFFLCPLTVVAVYSFLQKGLYGGVVWKFSIRAYAQVLTSGYGALLARTLLI
STGVTCLCVLLALPAGYALSRSRRQTFFLFLIIVPFWTNSLIRINAWITILGNQGFLNEALKSLGFITRSLPLLYHNGAV
IVVLTYMFLPYAIFPIFAAIDRFDFSLLESACDLGSTPTGAIIRVLLPNIKTGIATAVFFTFIPVFGSYTVPLLVGGKDS
YLIGNAIVDQVRIVGNWPLASAFAMLVTLAGGVGVLWILAESGACNTTPPPCGGRRSYA

Sequences:

>Translated_299_residues
MSSRLRVARRRTVGSLGFWCTLPLSAWLTVFFLCPLTVVAVYSFLQKGLYGGVVWKFSIRAYAQVLTSGYGALLARTLLI
STGVTCLCVLLALPAGYALSRSRRQTFFLFLIIVPFWTNSLIRINAWITILGNQGFLNEALKSLGFITRSLPLLYHNGAV
IVVLTYMFLPYAIFPIFAAIDRFDFSLLESACDLGSTPTGAIIRVLLPNIKTGIATAVFFTFIPVFGSYTVPLLVGGKDS
YLIGNAIVDQVRIVGNWPLASAFAMLVTLAGGVGVLWILAESGACNTTPPPCGGRRSYA
>Mature_298_residues
SSRLRVARRRTVGSLGFWCTLPLSAWLTVFFLCPLTVVAVYSFLQKGLYGGVVWKFSIRAYAQVLTSGYGALLARTLLIS
TGVTCLCVLLALPAGYALSRSRRQTFFLFLIIVPFWTNSLIRINAWITILGNQGFLNEALKSLGFITRSLPLLYHNGAVI
VVLTYMFLPYAIFPIFAAIDRFDFSLLESACDLGSTPTGAIIRVLLPNIKTGIATAVFFTFIPVFGSYTVPLLVGGKDSY
LIGNAIVDQVRIVGNWPLASAFAMLVTLAGGVGVLWILAESGACNTTPPPCGGRRSYA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=252, Percent_Identity=32.9365079365079, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1787080, Length=204, Percent_Identity=36.7647058823529, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1787713, Length=205, Percent_Identity=32.1951219512195, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32473; Mature: 32342

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRLRVARRRTVGSLGFWCTLPLSAWLTVFFLCPLTVVAVYSFLQKGLYGGVVWKFSIR
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHH
AYAQVLTSGYGALLARTLLISTGVTCLCVLLALPAGYALSRSRRQTFFLFLIIVPFWTNS
HHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
LIRINAWITILGNQGFLNEALKSLGFITRSLPLLYHNGAVIVVLTYMFLPYAIFPIFAAI
EEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHH
DRFDFSLLESACDLGSTPTGAIIRVLLPNIKTGIATAVFFTFIPVFGSYTVPLLVGGKDS
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
YLIGNAIVDQVRIVGNWPLASAFAMLVTLAGGVGVLWILAESGACNTTPPPCGGRRSYA
EEEHHHHHHHHHEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSRLRVARRRTVGSLGFWCTLPLSAWLTVFFLCPLTVVAVYSFLQKGLYGGVVWKFSIR
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHH
AYAQVLTSGYGALLARTLLISTGVTCLCVLLALPAGYALSRSRRQTFFLFLIIVPFWTNS
HHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
LIRINAWITILGNQGFLNEALKSLGFITRSLPLLYHNGAVIVVLTYMFLPYAIFPIFAAI
EEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHH
DRFDFSLLESACDLGSTPTGAIIRVLLPNIKTGIATAVFFTFIPVFGSYTVPLLVGGKDS
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
YLIGNAIVDQVRIVGNWPLASAFAMLVTLAGGVGVLWILAESGACNTTPPPCGGRRSYA
EEEHHHHHHHHHEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]