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The map label for this gene is miaA

Identifier: 189025862

GI number: 189025862

Start: 697149

End: 698099

Strand: Reverse

Name: miaA

Synonym: TPASS_0637

Alternate gene names: 189025862

Gene position: 698099-697149 (Counterclockwise)

Preceding gene: 189025863

Following gene: 189025861

Centisome position: 61.27

GC content: 52.68

Gene sequence:

>951_bases
ATGCGGTTGGAAACGCAGGCACTTGTACCGTACCCTGTTCGCTTTGACCGCAGCCACCATGATGCGCTGGTGGTCCTGGG
CGCTACCGCAACAGGTAAGACAGCGTTAGCAGTTGCGCTTGCCCAAAAATATCAGGGGGAAATTATTTCCGCCGATTCGC
GGCAGGTGTACCGTGGTCTGGATGTGGGAACGGGAAAGGACTTAGCTCTGTACGGGTCGGTCCCCTATCACCTGATAGAC
GTGTGTGATCCGTATGAGGAATACAATGTTTTCCGTTTCCAACAGGCAGTATATGGCATAGTGCCGAGTATACTCCGGGC
GCACAAGGTGCCAATTATTGTCGGTGGTACGGGTTTGTATCTTGATGCAGTGCTGCGTCAGTACGCGTTGGTACCTGTTG
AAAGAAATCAGGCGCTGCGCGCATCGCTCCGCGGAGCTTCTCTGTCGCATATGCGCGCGGTGTACTTTTCGTTAAAAGAC
TCCCATGCTGTTCACAACAAGACAGATTTAGAAGATCCTGCGCGTTTGATGCGCGCTATTGAGATTGCTGTATTCCATGC
AACGCACCCTGAGCTGCTCCAGCAGGCACGGGAAACGCGCCCGATGATGCGCGCGAAAGTGTATGGCATACAGTATCCAC
GCTCTATGTTGCGTGCTCGGATTCGAGCACGCCTCGAGCAGAGAATACGTGGGGGACTGATAGAGGAAGTGGCAGCGCTC
CACAAAGGCGGGGTTTCCTGGCAGCGTCTGGAATACTTTGGGTTGGAATATCGCTTCACTGCGCAGTATCTACAAGGGAT
CATTGCTACCCGTGATGAATATGTCGACCTACTTTTTAGAGCTATTAGCAGATTTGCAAAACGCCAGGAGACGTGGTTCC
GACGTATGCAAAGACTCGGGGTAAAAATTCACTGGCTCGTGCATACGGAAAACGGTTTTGTTCTCCGGTGA

Upstream 100 bases:

>100_bases
GGTGAATGGCTGTGGCGGGTTCCTTTGAGCGGAGTAGATTGCCGTTACGAGCACCATGAACGCGCCGTCGGAGTGGCGAA
GACAAGACTGCCCGGTCCCA

Downstream 100 bases:

>100_bases
AAAAACGATGATCGCTCATCGCACCGCTCCATAGGGTATGTGTGTGGCGAGTCGCTCGTGGTACGCACAGGCGTTAGTTC
TTTCTCTTTCAGCATTTGGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MRLETQALVPYPVRFDRSHHDALVVLGATATGKTALAVALAQKYQGEIISADSRQVYRGLDVGTGKDLALYGSVPYHLID
VCDPYEEYNVFRFQQAVYGIVPSILRAHKVPIIVGGTGLYLDAVLRQYALVPVERNQALRASLRGASLSHMRAVYFSLKD
SHAVHNKTDLEDPARLMRAIEIAVFHATHPELLQQARETRPMMRAKVYGIQYPRSMLRARIRARLEQRIRGGLIEEVAAL
HKGGVSWQRLEYFGLEYRFTAQYLQGIIATRDEYVDLLFRAISRFAKRQETWFRRMQRLGVKIHWLVHTENGFVLR

Sequences:

>Translated_316_residues
MRLETQALVPYPVRFDRSHHDALVVLGATATGKTALAVALAQKYQGEIISADSRQVYRGLDVGTGKDLALYGSVPYHLID
VCDPYEEYNVFRFQQAVYGIVPSILRAHKVPIIVGGTGLYLDAVLRQYALVPVERNQALRASLRGASLSHMRAVYFSLKD
SHAVHNKTDLEDPARLMRAIEIAVFHATHPELLQQARETRPMMRAKVYGIQYPRSMLRARIRARLEQRIRGGLIEEVAAL
HKGGVSWQRLEYFGLEYRFTAQYLQGIIATRDEYVDLLFRAISRFAKRQETWFRRMQRLGVKIHWLVHTENGFVLR
>Mature_316_residues
MRLETQALVPYPVRFDRSHHDALVVLGATATGKTALAVALAQKYQGEIISADSRQVYRGLDVGTGKDLALYGSVPYHLID
VCDPYEEYNVFRFQQAVYGIVPSILRAHKVPIIVGGTGLYLDAVLRQYALVPVERNQALRASLRGASLSHMRAVYFSLKD
SHAVHNKTDLEDPARLMRAIEIAVFHATHPELLQQARETRPMMRAKVYGIQYPRSMLRARIRARLEQRIRGGLIEEVAAL
HKGGVSWQRLEYFGLEYRFTAQYLQGIIATRDEYVDLLFRAISRFAKRQETWFRRMQRLGVKIHWLVHTENGFVLR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=313, Percent_Identity=24.6006389776358, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1790613, Length=293, Percent_Identity=30.3754266211604, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25144712, Length=127, Percent_Identity=38.5826771653543, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6324848, Length=298, Percent_Identity=25.503355704698, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24649830, Length=240, Percent_Identity=30.8333333333333, Blast_Score=95, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_TREPA (O83644)

Other databases:

- EMBL:   AE000520
- PIR:   B71301
- RefSeq:   NP_219074.1
- ProteinModelPortal:   O83644
- SMR:   O83644
- IntAct:   O83644
- GeneID:   2611718
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0637
- NMPDR:   fig|243276.1.peg.633
- TIGR:   TP_0637
- HOGENOM:   HBG559459
- OMA:   IYYGLEY
- ProtClustDB:   PRK00091
- BioCyc:   TPAL243276:TP_0637-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36174; Mature: 36174

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLETQALVPYPVRFDRSHHDALVVLGATATGKTALAVALAQKYQGEIISADSRQVYRGL
CCCCCCCCCCCCCEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEECCCCHHHHHCC
DVGTGKDLALYGSVPYHLIDVCDPYEEYNVFRFQQAVYGIVPSILRAHKVPIIVGGTGLY
CCCCCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHH
LDAVLRQYALVPVERNQALRASLRGASLSHMRAVYFSLKDSHAVHNKTDLEDPARLMRAI
HHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
EIAVFHATHPELLQQARETRPMMRAKVYGIQYPRSMLRARIRARLEQRIRGGLIEEVAAL
HHHHEECCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HKGGVSWQRLEYFGLEYRFTAQYLQGIIATRDEYVDLLFRAISRFAKRQETWFRRMQRLG
HHCCCCHHHHHHHCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VKIHWLVHTENGFVLR
CEEEEEEECCCCCEEC
>Mature Secondary Structure
MRLETQALVPYPVRFDRSHHDALVVLGATATGKTALAVALAQKYQGEIISADSRQVYRGL
CCCCCCCCCCCCCEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEECCCCHHHHHCC
DVGTGKDLALYGSVPYHLIDVCDPYEEYNVFRFQQAVYGIVPSILRAHKVPIIVGGTGLY
CCCCCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHH
LDAVLRQYALVPVERNQALRASLRGASLSHMRAVYFSLKDSHAVHNKTDLEDPARLMRAI
HHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
EIAVFHATHPELLQQARETRPMMRAKVYGIQYPRSMLRARIRARLEQRIRGGLIEEVAAL
HHHHEECCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HKGGVSWQRLEYFGLEYRFTAQYLQGIIATRDEYVDLLFRAISRFAKRQETWFRRMQRLG
HHCCCCHHHHHHHCCCCEEHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VKIHWLVHTENGFVLR
CEEEEEEECCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876