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The map label for this gene is nifS1

Identifier: 189025840

GI number: 189025840

Start: 668394

End: 669608

Strand: Direct

Name: nifS1

Synonym: TPASS_0614

Alternate gene names: 189025840

Gene position: 668394-669608 (Clockwise)

Preceding gene: 189025839

Following gene: 189025841

Centisome position: 58.66

GC content: 54.4

Gene sequence:

>1215_bases
ATGAGCGGCCCCAATTATAAAGCAGACTTTCCCCTGCTGTTGCGCAGTCCCCGCGTCCACTACCTAGACAGCGCGGCCAC
AACCCAACGCCCTGCGCCGGTGCTAGAGCGCGTTATGCACTACCACACCCATCTGAATGGGAACGCAGGCAGAGGCTCCC
ATGAACTTGCAGTTGAATCAGCGCTCCTTATAGAAAACACCCGGAAGAAAACAGCGCAGTTTATCAACGCAGCGCCAACG
CACGATATCGTTTTTACAAAGAGTTGCACCGAATCGCTCAACATCATCGCTCACTGTTACGCGCTGCCACGCCTGCGCGC
AGGAGACGAGATCGTTCTTGCTATCTCCAATCATCACGCAAATATCGTACCGTGGCAGCACGTGTGCCGCTGCACAGGTG
CAACGATACAGTGGCTGTATCCAGACGCCGAAGGAAATTTGGATATACAAGAAGCGCAGAAAAAGATACGAGCGTGCACT
AAGATTGTGTCCTTCTCTGCCGTTGTCAATGCCACCGGCGCGGTAAATCCTGCACAGGAATTGACCGCACTTGCACACCA
AGTCGGTGCAGTGGTGGTCATTGACGGAGCACAGGCTATGGTGCACGGCGTGCCAAATGTTGCAGATTTAGGCTGCGACT
TCTTTGTTTTCTCCGGCCATAAGATGTTCTCTCTTTTTGGCGTCGGCGTATTGTGCGCACCGCACACGCTCCTGGAATCC
ATGCCTCCTTTTTTGTATGGGGGAGGCATGGTGGATTTTGTGACTGAACAGGAAAGTGTCTTTAAGGGTGCGCCGCATAA
GTACGAGGGAGGTAGCGCGAATACTGCAGCTGTCGTGTCACTGTGTGCAGCGATTGAGTATTGCGAGTCCCTAGAGAGCA
GCGCAGTCCGCGCGTCCGTACATGCGCTGGATGCTGCACTCCTTGCGCGGCTGGAGGAGCTTCCCTTCCTTGAAACGTAC
CATGCGCGCGCACGCGAGCGCCTAGGCATCATTGCATTCAACGTGAAGAACGTGCACTCGCACGATACTGCGCATATCTT
GGGCGAAGAAGGCGTGATGGTTCGCAGCGGCGATCACTGTAGTAAGCCTTTCATGACGCACTTGAGCATTCAGTCCTGTT
GCCGTGCAAGTTTCTGCATATACAATACCATGGAGGATGTAGAGGCGCTGACGCGTGCGTTGCACGCCGTGGGCAGGATT
TTTCAATGCAGCTAG

Upstream 100 bases:

>100_bases
TTAAACCCGTATTCGCCCTCATCGATGACGCTCACATCCGCGAGACACTCGTACGTAATTTTGACGAGAGCCTCGATCGC
GCACATAGGAAGCGCAAGGC

Downstream 100 bases:

>100_bases
GCCAGTGCGCCTTCCTGGCATTCGCTGCCTTTCTCTAAGGAGATCTCCCTATGAACGCAGAAGCGATATACCGACAGGTG
CTGCTCGAGTACGCACGCAA

Product: nitrogen fixation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSGPNYKADFPLLLRSPRVHYLDSAATTQRPAPVLERVMHYHTHLNGNAGRGSHELAVESALLIENTRKKTAQFINAAPT
HDIVFTKSCTESLNIIAHCYALPRLRAGDEIVLAISNHHANIVPWQHVCRCTGATIQWLYPDAEGNLDIQEAQKKIRACT
KIVSFSAVVNATGAVNPAQELTALAHQVGAVVVIDGAQAMVHGVPNVADLGCDFFVFSGHKMFSLFGVGVLCAPHTLLES
MPPFLYGGGMVDFVTEQESVFKGAPHKYEGGSANTAAVVSLCAAIEYCESLESSAVRASVHALDAALLARLEELPFLETY
HARARERLGIIAFNVKNVHSHDTAHILGEEGVMVRSGDHCSKPFMTHLSIQSCCRASFCIYNTMEDVEALTRALHAVGRI
FQCS

Sequences:

>Translated_404_residues
MSGPNYKADFPLLLRSPRVHYLDSAATTQRPAPVLERVMHYHTHLNGNAGRGSHELAVESALLIENTRKKTAQFINAAPT
HDIVFTKSCTESLNIIAHCYALPRLRAGDEIVLAISNHHANIVPWQHVCRCTGATIQWLYPDAEGNLDIQEAQKKIRACT
KIVSFSAVVNATGAVNPAQELTALAHQVGAVVVIDGAQAMVHGVPNVADLGCDFFVFSGHKMFSLFGVGVLCAPHTLLES
MPPFLYGGGMVDFVTEQESVFKGAPHKYEGGSANTAAVVSLCAAIEYCESLESSAVRASVHALDAALLARLEELPFLETY
HARARERLGIIAFNVKNVHSHDTAHILGEEGVMVRSGDHCSKPFMTHLSIQSCCRASFCIYNTMEDVEALTRALHAVGRI
FQCS
>Mature_403_residues
SGPNYKADFPLLLRSPRVHYLDSAATTQRPAPVLERVMHYHTHLNGNAGRGSHELAVESALLIENTRKKTAQFINAAPTH
DIVFTKSCTESLNIIAHCYALPRLRAGDEIVLAISNHHANIVPWQHVCRCTGATIQWLYPDAEGNLDIQEAQKKIRACTK
IVSFSAVVNATGAVNPAQELTALAHQVGAVVVIDGAQAMVHGVPNVADLGCDFFVFSGHKMFSLFGVGVLCAPHTLLESM
PPFLYGGGMVDFVTEQESVFKGAPHKYEGGSANTAAVVSLCAAIEYCESLESSAVRASVHALDAALLARLEELPFLETYH
ARARERLGIIAFNVKNVHSHDTAHILGEEGVMVRSGDHCSKPFMTHLSIQSCCRASFCIYNTMEDVEALTRALHAVGRIF
QCS

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=280, Percent_Identity=27.1428571428571, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1787970, Length=399, Percent_Identity=36.5914786967419, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1789175, Length=390, Percent_Identity=35.3846153846154, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI48994898, Length=231, Percent_Identity=26.8398268398268, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=237, Percent_Identity=26.5822784810127, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI193211090, Length=377, Percent_Identity=23.6074270557029, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6319831, Length=233, Percent_Identity=28.755364806867, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI20129463, Length=237, Percent_Identity=29.535864978903, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CSD_TREPA (O83623)

Other databases:

- EMBL:   AE000520
- PIR:   C71303
- RefSeq:   NP_219052.1
- ProteinModelPortal:   O83623
- GeneID:   2611241
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0614
- NMPDR:   fig|243276.1.peg.611
- TIGR:   TP_0614
- HOGENOM:   HBG635316
- OMA:   YDSTWKE
- ProtClustDB:   CLSK218789
- BioCyc:   TPAL243276:TP_0614-MONOMER
- BRENDA:   2.8.1.7
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010970
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR01979

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 43852; Mature: 43720

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 360-360

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPNYKADFPLLLRSPRVHYLDSAATTQRPAPVLERVMHYHTHLNGNAGRGSHELAVES
CCCCCCCCCCCCEECCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ALLIENTRKKTAQFINAAPTHDIVFTKSCTESLNIIAHCYALPRLRAGDEIVLAISNHHA
HHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
NIVPWQHVCRCTGATIQWLYPDAEGNLDIQEAQKKIRACTKIVSFSAVVNATGAVNPAQE
CCCCHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LTALAHQVGAVVVIDGAQAMVHGVPNVADLGCDFFVFSGHKMFSLFGVGVLCAPHTLLES
HHHHHHHHCEEEEEECCHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHHHHHCCHHHHHH
MPPFLYGGGMVDFVTEQESVFKGAPHKYEGGSANTAAVVSLCAAIEYCESLESSAVRASV
CCCCEECCCEEEEECCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HALDAALLARLEELPFLETYHARARERLGIIAFNVKNVHSHDTAHILGEEGVMVRSGDHC
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCEEECCCCEEEECCCCC
SKPFMTHLSIQSCCRASFCIYNTMEDVEALTRALHAVGRIFQCS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGPNYKADFPLLLRSPRVHYLDSAATTQRPAPVLERVMHYHTHLNGNAGRGSHELAVES
CCCCCCCCCCCEECCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ALLIENTRKKTAQFINAAPTHDIVFTKSCTESLNIIAHCYALPRLRAGDEIVLAISNHHA
HHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
NIVPWQHVCRCTGATIQWLYPDAEGNLDIQEAQKKIRACTKIVSFSAVVNATGAVNPAQE
CCCCHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LTALAHQVGAVVVIDGAQAMVHGVPNVADLGCDFFVFSGHKMFSLFGVGVLCAPHTLLES
HHHHHHHHCEEEEEECCHHHHHCCCCHHHCCCCEEEECCCHHHHHHHHHHHHCCHHHHHH
MPPFLYGGGMVDFVTEQESVFKGAPHKYEGGSANTAAVVSLCAAIEYCESLESSAVRASV
CCCCEECCCEEEEECCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HALDAALLARLEELPFLETYHARARERLGIIAFNVKNVHSHDTAHILGEEGVMVRSGDHC
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCEEECCCCEEEECCCCC
SKPFMTHLSIQSCCRASFCIYNTMEDVEALTRALHAVGRIFQCS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876