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The map label for this gene is eda

Identifier: 189025796

GI number: 189025796

Start: 617866

End: 618498

Strand: Reverse

Name: eda

Synonym: TPASS_0568

Alternate gene names: 189025796

Gene position: 618498-617866 (Counterclockwise)

Preceding gene: 189025797

Following gene: 189025795

Centisome position: 54.28

GC content: 54.98

Gene sequence:

>633_bases
GTGATTACGATTTTCGAAGCGCTTGAGCGTGTGCGCGTCATTCCGGTGGTGACGCTTGAGCGCGTGGAAGACGCAGTGCC
GCTTGCACGCGCCTTGATAACAGGTGGTATCAGGTGTATGGAAGTAACATTTCGAACGTTGGTTGCTGCGGAGGCGATTG
CGGCAATCCGTCAGGAATGTGCTGATGTGTTACTTGGTGCAGGAACCGTACTGACGGTAGAGCAGGCGCAGCAGGCGCAG
GCAGCAGGTGCGCAGTTTGTGGTCAGTCCCGGTTTTAATCCGCGGGTTGTTGCGCACTGTTTGGGGCATGGCGTTCCGAT
CATACCGGGGATAGCATCTGCAACAGAAATTGAGCGTGCGCTTGAGTTTGGTATTTCGGTAGTAAAGTTTTTCCCCGCTG
AGCTTTTGGGAGGTACGGCAATGATGAGTGCGCTCGCACGTCCCTACACGGCGGTGCGTTTTGTGCCTACGGGGGGAATT
CATCTTAATAATCTTGCTGAGTATGTGGCGCATCCTCGGGTGCTCGCCTGTGGGGGCAGTTGGATGGTACCGGCGCAGTC
AATAGCGGCAGGAGATTTCTCGCAGGTTACTGCACTTTCTCAGCAGACGTTACAGATTGTCGGGGTAATGTAG

Upstream 100 bases:

>100_bases
TATACGCAGATCTTGATTTTTGATCATAAAGGACATTAGCTTAGGCGGGTGCACTGCATGTGGTGTCTGAAAAGATGTCC
GCGGGCAAGAGGAGGGAATT

Downstream 100 bases:

>100_bases
GGGGTGGTGGCTCAAACGTTGTCTTTCTCAGAGAGGGCACGTATACTGCCGGCGGCACTCAGGTGCGGGAGGTAACTGGT
GCGCAGAAACTCGAGGACGA

Product: 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxyphosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MITIFEALERVRVIPVVTLERVEDAVPLARALITGGIRCMEVTFRTLVAAEAIAAIRQECADVLLGAGTVLTVEQAQQAQ
AAGAQFVVSPGFNPRVVAHCLGHGVPIIPGIASATEIERALEFGISVVKFFPAELLGGTAMMSALARPYTAVRFVPTGGI
HLNNLAEYVAHPRVLACGGSWMVPAQSIAAGDFSQVTALSQQTLQIVGVM

Sequences:

>Translated_210_residues
MITIFEALERVRVIPVVTLERVEDAVPLARALITGGIRCMEVTFRTLVAAEAIAAIRQECADVLLGAGTVLTVEQAQQAQ
AAGAQFVVSPGFNPRVVAHCLGHGVPIIPGIASATEIERALEFGISVVKFFPAELLGGTAMMSALARPYTAVRFVPTGGI
HLNNLAEYVAHPRVLACGGSWMVPAQSIAAGDFSQVTALSQQTLQIVGVM
>Mature_210_residues
MITIFEALERVRVIPVVTLERVEDAVPLARALITGGIRCMEVTFRTLVAAEAIAAIRQECADVLLGAGTVLTVEQAQQAQ
AAGAQFVVSPGFNPRVVAHCLGHGVPIIPGIASATEIERALEFGISVVKFFPAELLGGTAMMSALARPYTAVRFVPTGGI
HLNNLAEYVAHPRVLACGGSWMVPAQSIAAGDFSQVTALSQQTLQIVGVM

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family

Homologues:

Organism=Escherichia coli, GI1788156, Length=184, Percent_Identity=42.9347826086957, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI48994954, Length=148, Percent_Identity=30.4054054054054, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ALKH_TREPA (O83578)

Other databases:

- EMBL:   AE000520
- PIR:   F71308
- RefSeq:   NP_219007.1
- ProteinModelPortal:   O83578
- SMR:   O83578
- GeneID:   2611476
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0568
- NMPDR:   fig|243276.1.peg.566
- TIGR:   TP_0568
- HOGENOM:   HBG670118
- OMA:   MKFFPAE
- ProtClustDB:   CLSK2300246
- BioCyc:   TPAL243276:TP_0568-MONOMER
- BRENDA:   4.1.2.14
- BRENDA:   4.1.3.16
- GO:   GO:0005737
- InterPro:   IPR000887
- InterPro:   IPR013785
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR01182

Pfam domain/function: PF01081 Aldolase

EC number: =4.1.3.16; =4.1.2.14

Molecular weight: Translated: 22074; Mature: 22074

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: ACT_SITE 41-41 ACT_SITE 45-45 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIFEALERVRVIPVVTLERVEDAVPLARALITGGIRCMEVTFRTLVAAEAIAAIRQEC
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ADVLLGAGTVLTVEQAQQAQAAGAQFVVSPGFNPRVVAHCLGHGVPIIPGIASATEIERA
HHHHHCCCCEEEHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
LEFGISVVKFFPAELLGGTAMMSALARPYTAVRFVPTGGIHLNNLAEYVAHPRVLACGGS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHCCCCEEEECCC
WMVPAQSIAAGDFSQVTALSQQTLQIVGVM
CCCCHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITIFEALERVRVIPVVTLERVEDAVPLARALITGGIRCMEVTFRTLVAAEAIAAIRQEC
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ADVLLGAGTVLTVEQAQQAQAAGAQFVVSPGFNPRVVAHCLGHGVPIIPGIASATEIERA
HHHHHCCCCEEEHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHH
LEFGISVVKFFPAELLGGTAMMSALARPYTAVRFVPTGGIHLNNLAEYVAHPRVLACGGS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHCCCCEEEECCC
WMVPAQSIAAGDFSQVTALSQQTLQIVGVM
CCCCHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876