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The map label for this gene is tig

Identifier: 189025736

GI number: 189025736

Start: 542563

End: 543891

Strand: Direct

Name: tig

Synonym: TPASS_0506

Alternate gene names: 189025736

Gene position: 542563-543891 (Clockwise)

Preceding gene: 189025734

Following gene: 189025737

Centisome position: 47.62

GC content: 54.25

Gene sequence:

>1329_bases
GTGGAACTTCAAAAAAAATTCACCGCGCTTGCACAATCTCAGGTTGAGCTAGAGGTGGTCGTCGCGCGCGAGGATGCGCA
GCGGCATTATCAGCGTTTTGTTGAGGAATATCTTGAGCGTGCGCGGCTTCCTGGTTTCCGCAAGGGGAAAGTTCCTCTTG
CAGTGCTTGAGCGGAAGTATGGAAGTGCTATTCGGCAAGATGCAGCGGCGGCCCTCATGGAAAAAGCTCTGGAGGAGGGG
TTTGCCCAGGCGTCGCAGGACAGTCAGCCTCTTCCTATCTCCCGTCCTTCGCTTAAGAAAAAGCCAGTGTTCGATCCTGA
CGAGGATTTCTCTTTTGCTGTCATATACGACGTATTTCCTTCCGTTGAACTACGCAACACATCGGGCTTTTCGCTGTCCG
TGCCCACTGTGTCGGTCACAGAAGAGGACGTCTCTCGAGAGTTAACGCGTATTCAGGAGCGCAATGCGCTGGTTACTGAT
AAGGGTGCAGATTCGTGTGCAGAGGTAGGGGACATTGCCACCGTCGATTATCACGAGGTTGACGATTCAGGTGCCGTTCG
TCCGGGTACTGAGCGCGCCGGCGTTGTCTTTACGCTTGGGGTGGAGGAAGGTCCCTTTGCGCTTGGGCAAGATATATTGG
GTATGAAATTAGGACAGCGGTGTCTCTTTGCTAAAAGGGCCGGTATGCTGAAGGACGAAGCCGCTCAGGTGAGGGTAACG
CTTAAGGCGCTCAAGCAGCGTCAGTTGCCGAGTCTTGATGATGAGCTTGCGCAAGATGTGAGCGATGCTTTTCGCACACT
TGACGATCTGACACGGAGTGTGCGGCAAAACCTCGCGGAAGCGTTGGAGGCAGCGCTGCACGAGTATAAGAGGCGGCAGC
TGTTGCGTATATTGGTGCGGGAGAATCCTTTTTCTCTGCCGGAATCTTTGGTTGTGGGGGAGATGGAGTCTCGTTGGGCG
TTGGTGATGCGTCAGTTTGGGGTGAGCCTGTCGGGCACCCCGCAGAATAAGCTGCAGTTTTTTCAGCAGTGGCGTCCGGA
GGTGGAAGAGCACCTGAAGCAGCGTGTGATTGTTGAGCTCCTTCTTAAGCAGGAGCAGGTGTCTGTTTCTGCTGAGGAAA
TTGAGACGGAGTACGTACGCATTGCGTCCAAAACAGGTTCCAAAGAGGAGCGGGTACGCGAGTATTATGCAGGGGAGGAA
AAACGGCGTGCGCTGTGTGAAGGAATACGAGAACGGAAGCTGTGCCAGAAATTGCTCGGACGATGTGTGACTGAGTGTGG
GCCGGAGCAGTCTTTGACTGACTTTCTTCAGGAGCAGTCCCGTGCGTGA

Upstream 100 bases:

>100_bases
TTCGATCCCCGTTTCCCGCTTTTTCAGCGCTTTGCTGCATCCTGCTTGGACCGTGCGAGGCGTTGCGGACGTAGGTATTT
CTTCCTAAGGATCTGGGCTT

Downstream 100 bases:

>100_bases
ACGTATGCATAATCTGGTTCCCTACGTGATTGAACAGTCGGGGGGTGGAGAGCGGAGCTATGACATTTTTTCCCGCTTGT
TGAAGGACCGTATTATTTTC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MELQKKFTALAQSQVELEVVVAREDAQRHYQRFVEEYLERARLPGFRKGKVPLAVLERKYGSAIRQDAAAALMEKALEEG
FAQASQDSQPLPISRPSLKKKPVFDPDEDFSFAVIYDVFPSVELRNTSGFSLSVPTVSVTEEDVSRELTRIQERNALVTD
KGADSCAEVGDIATVDYHEVDDSGAVRPGTERAGVVFTLGVEEGPFALGQDILGMKLGQRCLFAKRAGMLKDEAAQVRVT
LKALKQRQLPSLDDELAQDVSDAFRTLDDLTRSVRQNLAEALEAALHEYKRRQLLRILVRENPFSLPESLVVGEMESRWA
LVMRQFGVSLSGTPQNKLQFFQQWRPEVEEHLKQRVIVELLLKQEQVSVSAEEIETEYVRIASKTGSKEERVREYYAGEE
KRRALCEGIRERKLCQKLLGRCVTECGPEQSLTDFLQEQSRA

Sequences:

>Translated_442_residues
MELQKKFTALAQSQVELEVVVAREDAQRHYQRFVEEYLERARLPGFRKGKVPLAVLERKYGSAIRQDAAAALMEKALEEG
FAQASQDSQPLPISRPSLKKKPVFDPDEDFSFAVIYDVFPSVELRNTSGFSLSVPTVSVTEEDVSRELTRIQERNALVTD
KGADSCAEVGDIATVDYHEVDDSGAVRPGTERAGVVFTLGVEEGPFALGQDILGMKLGQRCLFAKRAGMLKDEAAQVRVT
LKALKQRQLPSLDDELAQDVSDAFRTLDDLTRSVRQNLAEALEAALHEYKRRQLLRILVRENPFSLPESLVVGEMESRWA
LVMRQFGVSLSGTPQNKLQFFQQWRPEVEEHLKQRVIVELLLKQEQVSVSAEEIETEYVRIASKTGSKEERVREYYAGEE
KRRALCEGIRERKLCQKLLGRCVTECGPEQSLTDFLQEQSRA
>Mature_442_residues
MELQKKFTALAQSQVELEVVVAREDAQRHYQRFVEEYLERARLPGFRKGKVPLAVLERKYGSAIRQDAAAALMEKALEEG
FAQASQDSQPLPISRPSLKKKPVFDPDEDFSFAVIYDVFPSVELRNTSGFSLSVPTVSVTEEDVSRELTRIQERNALVTD
KGADSCAEVGDIATVDYHEVDDSGAVRPGTERAGVVFTLGVEEGPFALGQDILGMKLGQRCLFAKRAGMLKDEAAQVRVT
LKALKQRQLPSLDDELAQDVSDAFRTLDDLTRSVRQNLAEALEAALHEYKRRQLLRILVRENPFSLPESLVVGEMESRWA
LVMRQFGVSLSGTPQNKLQFFQQWRPEVEEHLKQRVIVELLLKQEQVSVSAEEIETEYVRIASKTGSKEERVREYYAGEE
KRRALCEGIRERKLCQKLLGRCVTECGPEQSLTDFLQEQSRA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=331, Percent_Identity=27.7945619335347, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_TREPA (O83519)

Other databases:

- EMBL:   AE000520
- PIR:   A71314
- RefSeq:   NP_218947.1
- ProteinModelPortal:   O83519
- IntAct:   O83519
- GeneID:   2611712
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0506
- NMPDR:   fig|243276.1.peg.506
- TIGR:   TP_0506
- HOGENOM:   HBG732042
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   TPAL243276:TP_0506-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49939; Mature: 49939

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELQKKFTALAQSQVELEVVVAREDAQRHYQRFVEEYLERARLPGFRKGKVPLAVLERKY
CCHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
GSAIRQDAAAALMEKALEEGFAQASQDSQPLPISRPSLKKKPVFDPDEDFSFAVIYDVFP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHEECCC
SVELRNTSGFSLSVPTVSVTEEDVSRELTRIQERNALVTDKGADSCAEVGDIATVDYHEV
CCEEECCCCCEEECCEEECCHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCCCCCC
DDSGAVRPGTERAGVVFTLGVEEGPFALGQDILGMKLGQRCLFAKRAGMLKDEAAQVRVT
CCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LKALKQRQLPSLDDELAQDVSDAFRTLDDLTRSVRQNLAEALEAALHEYKRRQLLRILVR
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENPFSLPESLVVGEMESRWALVMRQFGVSLSGTPQNKLQFFQQWRPEVEEHLKQRVIVEL
CCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHH
LLKQEQVSVSAEEIETEYVRIASKTGSKEERVREYYAGEEKRRALCEGIRERKLCQKLLG
HHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RCVTECGPEQSLTDFLQEQSRA
HHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MELQKKFTALAQSQVELEVVVAREDAQRHYQRFVEEYLERARLPGFRKGKVPLAVLERKY
CCHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
GSAIRQDAAAALMEKALEEGFAQASQDSQPLPISRPSLKKKPVFDPDEDFSFAVIYDVFP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHEECCC
SVELRNTSGFSLSVPTVSVTEEDVSRELTRIQERNALVTDKGADSCAEVGDIATVDYHEV
CCEEECCCCCEEECCEEECCHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCCCCCC
DDSGAVRPGTERAGVVFTLGVEEGPFALGQDILGMKLGQRCLFAKRAGMLKDEAAQVRVT
CCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LKALKQRQLPSLDDELAQDVSDAFRTLDDLTRSVRQNLAEALEAALHEYKRRQLLRILVR
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENPFSLPESLVVGEMESRWALVMRQFGVSLSGTPQNKLQFFQQWRPEVEEHLKQRVIVEL
CCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHH
LLKQEQVSVSAEEIETEYVRIASKTGSKEERVREYYAGEEKRRALCEGIRERKLCQKLLG
HHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
RCVTECGPEQSLTDFLQEQSRA
HHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876