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The map label for this gene is dnaG

Identifier: 189025723

GI number: 189025723

Start: 527509

End: 529326

Strand: Direct

Name: dnaG

Synonym: TPASS_0492

Alternate gene names: 189025723

Gene position: 527509-529326 (Clockwise)

Preceding gene: 189025722

Following gene: 189025724

Centisome position: 46.29

GC content: 51.1

Gene sequence:

>1818_bases
GTGGCTCGGATTTCTGCGCACGTTATTGATGCGATTGCTGATCGTGTGGATTTGGTTTCGCTGGTGGGAAATTACACGCA
TCTGGAGCGGCGTGGGGATGACTGGTGGGGTCGCTGTCCATTTCATCATGAGCGTACGCCTTCGTTTCATGTGGTGCCGG
ATAAAAAGATGTACTATTGCTTTGGGTGTGGGGTTGGTGGATCCACTATTAAGTTTTTTATGGAAATCGAGAAAATTGAT
TTCCACGAAGCGGCAGTGCGTCTTGCAAAGCGTGCAGGAATCGAGATGTCCTTTGAGGACGGGGTGCACGCTCCTTCTGC
TCATGCTTCCTTTACAATGCAGCTGTGTGAAGTGTATCAGCGCATTGCAGAGACGTTCCATCACGTACTTATGCACACCG
CGCAAGGAGCGCGTGCGCGCGCGTACCTAGCCTCGCGCAAGGTAACGGATGATTCAATACGCACCTTTAAGCTCGGGTAC
GCTCCGCCGGATCCGGTATGGTTGTTTCAATTTTTAAGGCACAAGGGATACTCCCCCGAGTTTCTGGCCCGTTCTGGGTT
GTTTGCAAAAAAAAGCGAGCGTATCGCCGTTTTTTCAGATCGGATCATGTATCCGATTGCCGACCGCTACGGTCAGGTTA
TCGCATTCGGAGCGCGCGCCTTGGGGACTGCACCTGCAAAGTATTTGAACACGGCAGATATGCCACAGTATAAAAAGGGT
GAGCACTTGTTTGCGTTTCACTGTGCTCTTTCTCAGATGAGAAAGACGCGCGCGGCGATTATATGTGAAGGATACATGGA
TGTTATCGCGTTTCATCAGGCGCAGTTGACGTATGCTGTTGCGCCTTTAGGCGCATTGCTGACGAAAAGCCAGGCACGTT
TGATGCGTTCGTTTGTCGATCGAATATATATGTGTTTTGATGCCGACGGAGCAGGCAGAGCGGCAACGTACAAGGCGATT
TTGTTGTGTCGTTCCTTGGGTTTTGAGGTACGGATAGTAGAATTGAATGGAGGTACTGATCCTGCAGAATGTGCGTGTAT
AGAAGGAGAGGACGCTTTGAGAAAAAGCGTAGAACGGAGCACTACTGACGCGCAGTATTTGATACGGTGTGCACGCCATG
AGCACAGTCACCTTGGTGCAGATGACACATCGCGTGCGGTGTCCTTTTTATTCCCTTATCTGAGTGTCTTGGACTCTGCC
ATTCAGCGTGAGCAAGTCATGCAGGATATTGCGATGGCGTTTGGCATTCGCATACAGGCGGTGCACGCAGATTACCTGCG
TTATGTGTCCCGTACCACGCAGAAAGGGACAACAGGGAATTGTGTTCTGTCTGTACAGGGAACAGCGATACAGGTGAAGG
AGCCTGCTACGGGAGTACGCACTGCGCAGCTGCGTTTGGTACTAGCGGTGGTAGCAAATCCTGAGTTATTTGAGCTCCTG
CGGGAGAGTGTGTGTGCAGATGACTTTGAAGATCCTATGGCAAAAGAGTTATTCATAATCCTAGAGGAGTGTTATCGTGC
AGACACGCGTGCAAGTCCGCATGTTCTTTCGTGTTGTACAACCGACGAGTTAAGGAAACTCGTGAGCGAGGCAATTGTCT
GTGGTGAGTTCTCTTGCAATGCGCCGCAGATTGTGCGTGACGGTGTTGCGCTCGTGCGTCGTAATAGACTGCTGAAGGAG
CGAGAATCGCTCGTAGGGCGGCTGCGCCGATTTGGGGATGCATCTTCGGGTGAGGAGTGCGGGTCTATGCAGGAGCTTAT
GATGGAAAAGCAGCGGGTTGATGAGGAGTTAGAAAGGTTGAAAGGGGTGAGGAAATGA

Upstream 100 bases:

>100_bases
TCACCGATCCGCAGCGGGCACGCACACGTTCACCAAGACGTTGGACGAGCATGATCAAGCTGGGCTCATGCTGCTAAAGA
AAAATACGGGAATGTAGGCA

Downstream 100 bases:

>100_bases
TGGAGCTGTCACGTACTCCTGCGGTGATGCGCCTGTTAGAATATGCGAGGGAGAAGAAGGCTATAACGCATGATGAGGTC
GAGAACATACTCGCGCACTA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MARISAHVIDAIADRVDLVSLVGNYTHLERRGDDWWGRCPFHHERTPSFHVVPDKKMYYCFGCGVGGSTIKFFMEIEKID
FHEAAVRLAKRAGIEMSFEDGVHAPSAHASFTMQLCEVYQRIAETFHHVLMHTAQGARARAYLASRKVTDDSIRTFKLGY
APPDPVWLFQFLRHKGYSPEFLARSGLFAKKSERIAVFSDRIMYPIADRYGQVIAFGARALGTAPAKYLNTADMPQYKKG
EHLFAFHCALSQMRKTRAAIICEGYMDVIAFHQAQLTYAVAPLGALLTKSQARLMRSFVDRIYMCFDADGAGRAATYKAI
LLCRSLGFEVRIVELNGGTDPAECACIEGEDALRKSVERSTTDAQYLIRCARHEHSHLGADDTSRAVSFLFPYLSVLDSA
IQREQVMQDIAMAFGIRIQAVHADYLRYVSRTTQKGTTGNCVLSVQGTAIQVKEPATGVRTAQLRLVLAVVANPELFELL
RESVCADDFEDPMAKELFIILEECYRADTRASPHVLSCCTTDELRKLVSEAIVCGEFSCNAPQIVRDGVALVRRNRLLKE
RESLVGRLRRFGDASSGEECGSMQELMMEKQRVDEELERLKGVRK

Sequences:

>Translated_605_residues
MARISAHVIDAIADRVDLVSLVGNYTHLERRGDDWWGRCPFHHERTPSFHVVPDKKMYYCFGCGVGGSTIKFFMEIEKID
FHEAAVRLAKRAGIEMSFEDGVHAPSAHASFTMQLCEVYQRIAETFHHVLMHTAQGARARAYLASRKVTDDSIRTFKLGY
APPDPVWLFQFLRHKGYSPEFLARSGLFAKKSERIAVFSDRIMYPIADRYGQVIAFGARALGTAPAKYLNTADMPQYKKG
EHLFAFHCALSQMRKTRAAIICEGYMDVIAFHQAQLTYAVAPLGALLTKSQARLMRSFVDRIYMCFDADGAGRAATYKAI
LLCRSLGFEVRIVELNGGTDPAECACIEGEDALRKSVERSTTDAQYLIRCARHEHSHLGADDTSRAVSFLFPYLSVLDSA
IQREQVMQDIAMAFGIRIQAVHADYLRYVSRTTQKGTTGNCVLSVQGTAIQVKEPATGVRTAQLRLVLAVVANPELFELL
RESVCADDFEDPMAKELFIILEECYRADTRASPHVLSCCTTDELRKLVSEAIVCGEFSCNAPQIVRDGVALVRRNRLLKE
RESLVGRLRRFGDASSGEECGSMQELMMEKQRVDEELERLKGVRK
>Mature_604_residues
ARISAHVIDAIADRVDLVSLVGNYTHLERRGDDWWGRCPFHHERTPSFHVVPDKKMYYCFGCGVGGSTIKFFMEIEKIDF
HEAAVRLAKRAGIEMSFEDGVHAPSAHASFTMQLCEVYQRIAETFHHVLMHTAQGARARAYLASRKVTDDSIRTFKLGYA
PPDPVWLFQFLRHKGYSPEFLARSGLFAKKSERIAVFSDRIMYPIADRYGQVIAFGARALGTAPAKYLNTADMPQYKKGE
HLFAFHCALSQMRKTRAAIICEGYMDVIAFHQAQLTYAVAPLGALLTKSQARLMRSFVDRIYMCFDADGAGRAATYKAIL
LCRSLGFEVRIVELNGGTDPAECACIEGEDALRKSVERSTTDAQYLIRCARHEHSHLGADDTSRAVSFLFPYLSVLDSAI
QREQVMQDIAMAFGIRIQAVHADYLRYVSRTTQKGTTGNCVLSVQGTAIQVKEPATGVRTAQLRLVLAVVANPELFELLR
ESVCADDFEDPMAKELFIILEECYRADTRASPHVLSCCTTDELRKLVSEAIVCGEFSCNAPQIVRDGVALVRRNRLLKER
ESLVGRLRRFGDASSGEECGSMQELMMEKQRVDEELERLKGVRK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Escherichia coli, GI1789447, Length=488, Percent_Identity=28.4836065573771, Blast_Score=209, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIM_TREPA (O83505)

Other databases:

- EMBL:   AE000520
- PIR:   D71318
- RefSeq:   NP_218933.1
- ProteinModelPortal:   O83505
- SMR:   O83505
- IntAct:   O83505
- GeneID:   2611380
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0492
- NMPDR:   fig|243276.1.peg.492
- TIGR:   TP_0492
- HOGENOM:   HBG732036
- OMA:   ICLPFNI
- ProtClustDB:   CLSK218730
- BioCyc:   TPAL243276:TP_0492-MONOMER
- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694
- Gene3D:   G3DSA:3.90.980.10
- Gene3D:   G3DSA:3.90.580.10
- SMART:   SM00493
- SMART:   SM00400
- TIGRFAMs:   TIGR01391

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2

EC number: 2.7.7.-

Molecular weight: Translated: 67894; Mature: 67763

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARISAHVIDAIADRVDLVSLVGNYTHLERRGDDWWGRCPFHHERTPSFHVVPDKKMYYC
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCEEEE
FGCGVGGSTIKFFMEIEKIDFHEAAVRLAKRAGIEMSFEDGVHAPSAHASFTMQLCEVYQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHH
RIAETFHHVLMHTAQGARARAYLASRKVTDDSIRTFKLGYAPPDPVWLFQFLRHKGYSPE
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCHH
FLARSGLFAKKSERIAVFSDRIMYPIADRYGQVIAFGARALGTAPAKYLNTADMPQYKKG
HHHHCCCCCCCCCCEEEECCCHHHHHHHHCCCEEEECHHHHCCCCHHHCCCCCCCCCCCC
EHLFAFHCALSQMRKTRAAIICEGYMDVIAFHQAQLTYAVAPLGALLTKSQARLMRSFVD
CEEEHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIYMCFDADGAGRAATYKAILLCRSLGFEVRIVELNGGTDPAECACIEGEDALRKSVERS
HHEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHEEEECCHHHHHHHHHHC
TTDAQYLIRCARHEHSHLGADDTSRAVSFLFPYLSVLDSAIQREQVMQDIAMAFGIRIQA
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
VHADYLRYVSRTTQKGTTGNCVLSVQGTAIQVKEPATGVRTAQLRLVLAVVANPELFELL
HHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHH
RESVCADDFEDPMAKELFIILEECYRADTRASPHVLSCCTTDELRKLVSEAIVCGEFSCN
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCC
APQIVRDGVALVRRNRLLKERESLVGRLRRFGDASSGEECGSMQELMMEKQRVDEELERL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KGVRK
HCCCC
>Mature Secondary Structure 
ARISAHVIDAIADRVDLVSLVGNYTHLERRGDDWWGRCPFHHERTPSFHVVPDKKMYYC
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCEEEE
FGCGVGGSTIKFFMEIEKIDFHEAAVRLAKRAGIEMSFEDGVHAPSAHASFTMQLCEVYQ
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHH
RIAETFHHVLMHTAQGARARAYLASRKVTDDSIRTFKLGYAPPDPVWLFQFLRHKGYSPE
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCHH
FLARSGLFAKKSERIAVFSDRIMYPIADRYGQVIAFGARALGTAPAKYLNTADMPQYKKG
HHHHCCCCCCCCCCEEEECCCHHHHHHHHCCCEEEECHHHHCCCCHHHCCCCCCCCCCCC
EHLFAFHCALSQMRKTRAAIICEGYMDVIAFHQAQLTYAVAPLGALLTKSQARLMRSFVD
CEEEHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIYMCFDADGAGRAATYKAILLCRSLGFEVRIVELNGGTDPAECACIEGEDALRKSVERS
HHEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHEEEECCHHHHHHHHHHC
TTDAQYLIRCARHEHSHLGADDTSRAVSFLFPYLSVLDSAIQREQVMQDIAMAFGIRIQA
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
VHADYLRYVSRTTQKGTTGNCVLSVQGTAIQVKEPATGVRTAQLRLVLAVVANPELFELL
HHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHH
RESVCADDFEDPMAKELFIILEECYRADTRASPHVLSCCTTDELRKLVSEAIVCGEFSCN
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCC
APQIVRDGVALVRRNRLLKERESLVGRLRRFGDASSGEECGSMQELMMEKQRVDEELERL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KGVRK
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876