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The map label for this gene is trmB

Identifier: 189025696

GI number: 189025696

Start: 493877

End: 494629

Strand: Reverse

Name: trmB

Synonym: TPASS_0464

Alternate gene names: 189025696

Gene position: 494629-493877 (Counterclockwise)

Preceding gene: 189025697

Following gene: 189025688

Centisome position: 43.41

GC content: 61.62

Gene sequence:

>753_bases
ATGACAAACGACAGTGCACGCATGCGGAAAGTTCTCACCTTTACGCGCAGATCAAACCGCATGACCGCATGCCAAAAACG
CGACTACCAGCACCTTGCGTCCCGCTGGATCATCCCGTATCAGAACACGGTGTTCGACTACGCTGCCGTATTCTGTTCAC
CGGCGGCACCTTCTGCGCCTGCCGGTGCTTTTCCCGCTCCGCAGGGGAAAACGGACGCAGTCGCTCCGGCGTGCGCGCCT
GCACCGCTGGTAGTAGAAATTGGCTTTGGTATGGGGAGCGCAACTGCGGCAATCGCGGCACGCAACCCGCACCTTTCCTA
CCTCGGCATCGAGGTGTACCGCGCAGGAATCGGACGACTGCTGAGAAAAATTGAAGCCGAACGTTTACACAACCTGCGCA
TCATCGAGCACGATGCACTCGACGTGCTGCGCACCATGATCGCACCGCAGACACTTGCAGGCCTGCACATTTTCTTTCCA
GACCCGTGGCCAAAAACGCGGCACCACAAGCGGCGCCTGCTGTATCGCCCGCGCACAGACCTACTCGCGCGCGCCCTTGC
ACCGGGTGGCTACCTATACGCGGTCACCGACTGGGCCGAGTACGCACGGCGCGCGCAGGAAGAGCTTGCGCGCACGCCCT
CGCTCACGTGGGCACCGCAGGGTGCGCGCCCCTGGCGCCCCGCCACGGAATTTGAACGCAAGGCGCAGACGCAGGGGCGC
GCCATTCACGAACTGTTTTTTATCAAAGCGTAA

Upstream 100 bases:

>100_bases
ACCTGCAGTGCGTGGTTGAGGACTTTCAGCGCTCCTGGCGCTTTATTTCACAACCTCACCGTTTATATCCTGAAGACGCC
CTGTCTGAGCAAGCGCCACC

Downstream 100 bases:

>100_bases
AGATGACCGGCGCGCGGCCTCCCGCCCCGCGCCTCCCTGCAGCACGCATCCGCCCGGGCTACTCCCGATTCCACTCCTTT
TTCTGGGCGTCAAATCTCCA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTNDSARMRKVLTFTRRSNRMTACQKRDYQHLASRWIIPYQNTVFDYAAVFCSPAAPSAPAGAFPAPQGKTDAVAPACAP
APLVVEIGFGMGSATAAIAARNPHLSYLGIEVYRAGIGRLLRKIEAERLHNLRIIEHDALDVLRTMIAPQTLAGLHIFFP
DPWPKTRHHKRRLLYRPRTDLLARALAPGGYLYAVTDWAEYARRAQEELARTPSLTWAPQGARPWRPATEFERKAQTQGR
AIHELFFIKA

Sequences:

>Translated_250_residues
MTNDSARMRKVLTFTRRSNRMTACQKRDYQHLASRWIIPYQNTVFDYAAVFCSPAAPSAPAGAFPAPQGKTDAVAPACAP
APLVVEIGFGMGSATAAIAARNPHLSYLGIEVYRAGIGRLLRKIEAERLHNLRIIEHDALDVLRTMIAPQTLAGLHIFFP
DPWPKTRHHKRRLLYRPRTDLLARALAPGGYLYAVTDWAEYARRAQEELARTPSLTWAPQGARPWRPATEFERKAQTQGR
AIHELFFIKA
>Mature_249_residues
TNDSARMRKVLTFTRRSNRMTACQKRDYQHLASRWIIPYQNTVFDYAAVFCSPAAPSAPAGAFPAPQGKTDAVAPACAPA
PLVVEIGFGMGSATAAIAARNPHLSYLGIEVYRAGIGRLLRKIEAERLHNLRIIEHDALDVLRTMIAPQTLAGLHIFFPD
PWPKTRHHKRRLLYRPRTDLLARALAPGGYLYAVTDWAEYARRAQEELARTPSLTWAPQGARPWRPATEFERKAQTQGRA
IHELFFIKA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=247, Percent_Identity=31.1740890688259, Blast_Score=134, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_TREPA (O83477)

Other databases:

- EMBL:   AE000520
- PIR:   B71321
- RefSeq:   NP_218905.1
- ProteinModelPortal:   O83477
- SMR:   O83477
- IntAct:   O83477
- MINT:   MINT-6479630
- GeneID:   2611075
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0464
- NMPDR:   fig|243276.1.peg.464
- TIGR:   TP_0464
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- ProtClustDB:   CLSK2300221
- BioCyc:   TPAL243276:TP_0464-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 28069; Mature: 27937

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: NA

Important sites: BINDING 86-86 BINDING 111-111 BINDING 138-138 BINDING 161-161 BINDING 165-165 BINDING 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDSARMRKVLTFTRRSNRMTACQKRDYQHLASRWIIPYQNTVFDYAAVFCSPAAPSAP
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
AGAFPAPQGKTDAVAPACAPAPLVVEIGFGMGSATAAIAARNPHLSYLGIEVYRAGIGRL
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHH
LRKIEAERLHNLRIIEHDALDVLRTMIAPQTLAGLHIFFPDPWPKTRHHKRRLLYRPRTD
HHHHHHHHHHCCEEEEHHHHHHHHHHHCCHHHCCEEEECCCCCCCCHHHHHHHHCCCHHH
LLARALAPGGYLYAVTDWAEYARRAQEELARTPSLTWAPQGARPWRPATEFERKAQTQGR
HHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHCCH
AIHELFFIKA
HHHHEEEECC
>Mature Secondary Structure 
TNDSARMRKVLTFTRRSNRMTACQKRDYQHLASRWIIPYQNTVFDYAAVFCSPAAPSAP
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
AGAFPAPQGKTDAVAPACAPAPLVVEIGFGMGSATAAIAARNPHLSYLGIEVYRAGIGRL
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHH
LRKIEAERLHNLRIIEHDALDVLRTMIAPQTLAGLHIFFPDPWPKTRHHKRRLLYRPRTD
HHHHHHHHHHCCEEEEHHHHHHHHHHHCCHHHCCEEEECCCCCCCCHHHHHHHHCCCHHH
LLARALAPGGYLYAVTDWAEYARRAQEELARTPSLTWAPQGARPWRPATEFERKAQTQGR
HHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHCCH
AIHELFFIKA
HHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876