Click here to switch to the map view.

The map label for this gene is gcpE

Identifier: 189025678

GI number: 189025678

Start: 473747

End: 474961

Strand: Direct

Name: gcpE

Synonym: TPASS_0446

Alternate gene names: 189025678

Gene position: 473747-474961 (Clockwise)

Preceding gene: 189025677

Following gene: 189025679

Centisome position: 41.58

GC content: 52.02

Gene sequence:

>1215_bases
GTGGATTCATCTGCCGGTGTGTCTCCCTGCAATAGTCCGTATGGGAGTGGACTTTTGGATGTTCCATTAAAATTAAGACC
GCGTGCGTCCCACTACCGTGCACGATCATTGGTAATTGGAGGAAAGGAACATGTGCGTGCGTTGCCTCTTGGGGGTGACG
CACCGATTCCTATCCAAACAATGTGGAAAGAACCGCTCATTGGTGCAGATCTCCAATCCATTGTGGACCGTCTTTTGGAA
CTTGAACAATTAGGGTGTGACGTTGTGCGTTTTGCCGTTCCTGACAGGGAATCGGCTGAGCTTTTTGTGGCGCTTTGCGC
GCGCACGCGTATGCCGCTTGTTGCAGACATTCACTTTGATTACCGGCTTGCGCTGCGGTGTATGGACGGACCGGTAGCGA
AAGTGCGTATTAACCCGGGGAATATCGGTGTGCGTGAGCGCGTGCGTGCGGTGGTAGAAAAGGCTCGAGCAACCGGCACT
GCTTTGCGTATTGGGGTGAATACCGGTTCTCTGCCGGGTGTGGTGAAGCGCGCAGTTGCGGCGCGTTACGCGGATGGGAT
GCAGTCAGTGAATGCGCGTGCAGAAGCGTTGGTGCAAACTGCCTTTGCAGAAGCTGCGTATCTAGATCAGTTACACTTTG
ATCGGGTTGTCCTTTCTCTGAAGGCTTCTACGGTTGCAGAGACGGTACGTGCCAACGAACTTTTTGCGCAGCAGTCTGAT
ATTCCCTTGCACCTTGGAGTAACAGAAGCAGGCCCGCTTGTTTCCGGTATTGTCAAAAGTACACTTGCATTTTCCCAATT
ACTGTCACGCAATATTGGTGCCACGGTGCGGGTGAGTCTTTCAGATAGCATGGAGCATGAGGTGCTGGCTGCGCGAGAAA
TTCTTGCTGAATGCGGTAAACGGGCTGGTGGGGTTCGTTTAGTGTCATGTCCGCGCTGTGGCAGGATTGGTTTTGACGTA
CACGCATTTGTGCGGAGGTGGCAAAAGGAACTGTTCAGTTTGAAAAAGGATATCACGGTTGCGGTTATGGGCTGTGTAGT
GAATGGTCCTGGAGAAGGAAAGCATGCGGATCTCGGTATCAGCGGTGCGGAGGATTCGGTGATTTTTTTTAAGCGGGGAA
AGATAGTGCGTCGCATTCAGGTACGTGATCTTTGCGCAGACGAGCGCACGCGCATAATAGACGCAGCGTTTAAAGAGGAA
TTGTCAAGTTTATGA

Upstream 100 bases:

>100_bases
AAGCACTCTGTGGAGTGGAAGTTGCGCAAAGTGTGCGTGCGCAAGTGGTGGCCCGGTGAATCAGAGGGACGAGCGTGCTG
CGCGGCAGCCAGAGGAAAAG

Downstream 100 bases:

>100_bases
ATAACCTGATCAAAGCATATGCGGCGGGTGTCATGAGTGCTGCGTTTCTTTTTGGGTCAGAGGGGCGGGTGCGCAGTGAA
TCCGATCGGGTGCGTGGGGA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MDSSAGVSPCNSPYGSGLLDVPLKLRPRASHYRARSLVIGGKEHVRALPLGGDAPIPIQTMWKEPLIGADLQSIVDRLLE
LEQLGCDVVRFAVPDRESAELFVALCARTRMPLVADIHFDYRLALRCMDGPVAKVRINPGNIGVRERVRAVVEKARATGT
ALRIGVNTGSLPGVVKRAVAARYADGMQSVNARAEALVQTAFAEAAYLDQLHFDRVVLSLKASTVAETVRANELFAQQSD
IPLHLGVTEAGPLVSGIVKSTLAFSQLLSRNIGATVRVSLSDSMEHEVLAAREILAECGKRAGGVRLVSCPRCGRIGFDV
HAFVRRWQKELFSLKKDITVAVMGCVVNGPGEGKHADLGISGAEDSVIFFKRGKIVRRIQVRDLCADERTRIIDAAFKEE
LSSL

Sequences:

>Translated_404_residues
MDSSAGVSPCNSPYGSGLLDVPLKLRPRASHYRARSLVIGGKEHVRALPLGGDAPIPIQTMWKEPLIGADLQSIVDRLLE
LEQLGCDVVRFAVPDRESAELFVALCARTRMPLVADIHFDYRLALRCMDGPVAKVRINPGNIGVRERVRAVVEKARATGT
ALRIGVNTGSLPGVVKRAVAARYADGMQSVNARAEALVQTAFAEAAYLDQLHFDRVVLSLKASTVAETVRANELFAQQSD
IPLHLGVTEAGPLVSGIVKSTLAFSQLLSRNIGATVRVSLSDSMEHEVLAAREILAECGKRAGGVRLVSCPRCGRIGFDV
HAFVRRWQKELFSLKKDITVAVMGCVVNGPGEGKHADLGISGAEDSVIFFKRGKIVRRIQVRDLCADERTRIIDAAFKEE
LSSL
>Mature_404_residues
MDSSAGVSPCNSPYGSGLLDVPLKLRPRASHYRARSLVIGGKEHVRALPLGGDAPIPIQTMWKEPLIGADLQSIVDRLLE
LEQLGCDVVRFAVPDRESAELFVALCARTRMPLVADIHFDYRLALRCMDGPVAKVRINPGNIGVRERVRAVVEKARATGT
ALRIGVNTGSLPGVVKRAVAARYADGMQSVNARAEALVQTAFAEAAYLDQLHFDRVVLSLKASTVAETVRANELFAQQSD
IPLHLGVTEAGPLVSGIVKSTLAFSQLLSRNIGATVRVSLSDSMEHEVLAAREILAECGKRAGGVRLVSCPRCGRIGFDV
HAFVRRWQKELFSLKKDITVAVMGCVVNGPGEGKHADLGISGAEDSVIFFKRGKIVRRIQVRDLCADERTRIIDAAFKEE
LSSL

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=349, Percent_Identity=38.10888252149, Blast_Score=230, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_TREPA (O83460)

Other databases:

- EMBL:   AE000520
- PIR:   F71324
- RefSeq:   NP_218886.1
- ProteinModelPortal:   O83460
- IntAct:   O83460
- GeneID:   2611004
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0446
- NMPDR:   fig|243276.1.peg.445
- TIGR:   TP_0446
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   CLSK218706
- BioCyc:   TPAL243276:TP_0446-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 43734; Mature: 43734

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSSAGVSPCNSPYGSGLLDVPLKLRPRASHYRARSLVIGGKEHVRALPLGGDAPIPIQT
CCCCCCCCCCCCCCCCCEEECCEEECCCCHHHHHHEEEECCHHHEEEECCCCCCCCCHHH
MWKEPLIGADLQSIVDRLLELEQLGCDVVRFAVPDRESAELFVALCARTRMPLVADIHFD
HHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCC
YRLALRCMDGPVAKVRINPGNIGVRERVRAVVEKARATGTALRIGVNTGSLPGVVKRAVA
EEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
ARYADGMQSVNARAEALVQTAFAEAAYLDQLHFDRVVLSLKASTVAETVRANELFAQQSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPLHLGVTEAGPLVSGIVKSTLAFSQLLSRNIGATVRVSLSDSMEHEVLAAREILAECGK
CCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
RAGGVRLVSCPRCGRIGFDVHAFVRRWQKELFSLKKDITVAVMGCVVNGPGEGKHADLGI
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCC
SGAEDSVIFFKRGKIVRRIQVRDLCADERTRIIDAAFKEELSSL
CCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSSAGVSPCNSPYGSGLLDVPLKLRPRASHYRARSLVIGGKEHVRALPLGGDAPIPIQT
CCCCCCCCCCCCCCCCCEEECCEEECCCCHHHHHHEEEECCHHHEEEECCCCCCCCCHHH
MWKEPLIGADLQSIVDRLLELEQLGCDVVRFAVPDRESAELFVALCARTRMPLVADIHFD
HHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCC
YRLALRCMDGPVAKVRINPGNIGVRERVRAVVEKARATGTALRIGVNTGSLPGVVKRAVA
EEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
ARYADGMQSVNARAEALVQTAFAEAAYLDQLHFDRVVLSLKASTVAETVRANELFAQQSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IPLHLGVTEAGPLVSGIVKSTLAFSQLLSRNIGATVRVSLSDSMEHEVLAAREILAECGK
CCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
RAGGVRLVSCPRCGRIGFDVHAFVRRWQKELFSLKKDITVAVMGCVVNGPGEGKHADLGI
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCC
SGAEDSVIFFKRGKIVRRIQVRDLCADERTRIIDAAFKEELSSL
CCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876