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The map label for this gene is dagA [C]

Identifier: 189025647

GI number: 189025647

Start: 442048

End: 443403

Strand: Direct

Name: dagA [C]

Synonym: TPASS_0414

Alternate gene names: 189025647

Gene position: 442048-443403 (Clockwise)

Preceding gene: 189025644

Following gene: 189025648

Centisome position: 38.79

GC content: 56.19

Gene sequence:

>1356_bases
ATGGGAGTAGTGGAATGGGTAGGTGAGTGGATGCACGCGGTGGTGTGGAGCTTTCCGATGGTGGTGCTGTTGCTGGGGAC
GGGGTGCTACGTGACGGTGTGTATGAAGTTTTTTCCTGTGGTGCGGCTGTGGTATGTATTAAGACAAACTATTGGGGGTC
GCGGAGGTAAGAAGGGCGGCAGTGGTGAGGTGAGTGCGTTCCGTGCGGTGTCCACTGCGCTTGCAGCGACGTTGGGGTCT
GGAAATATTGTAGGGGTTGCGACGGCGATTGCGATTGGGGGGCCTGGGGCGATATTTTGGATATGGGTGACGGGGATATT
CGGGATGGGAACGAAGTTCGTGGAAGTGGTGTTGGCGGTGTACTATCGGCGTCAGACTGGTGATGGGCGTTTTGTGGGGG
GGCCGATGTATTATCTGAAAGACGGAGTGGGAGTTCCAGGGGCTGGGGTACTTGCGAGTTTGTTCTGCATATTCAGTGTT
ATCGCGTCCTTTGGGATAGGAAATATGACGCAGTCGAACTCGGTGGCTCTAGTGTTCGAAGATGTGTTTTGTGTGGACGT
GCGGGTGACCGGGGCAGTGCTGATGGTCTTGGTAGGCTTAGTGAGCGTGGGTGGGTTAAAAAGTATCAGTTGGGTGACTG
GGGTAATGGTGCCTGGGATGGCGATTTTGTATGTATGTGTGGGCGTAGTGCTGGTGTGTTGCAATACGCGACAGCTGGTG
CCAGTGTGCTGGGATATCGTGTCCGGGGCGTTTGCCGGGACTGCAGCAGTTGGGGGGTTTGCAGGGAGTGTGGTGCGTCA
AGCGATAGCGGTAGGTATTAGCCGGGGGGTAGCGGTGAACGAGGCAGGGCTTGGGACTGCTCCTATTGCGCATGCGGCGG
CTATTACAGACCATCCAGTGCGACAGGGCTTGTGGGGTATCTTTGAAGTATTTGTGGGGACAATGGTGGTATCTTCGGTG
ACGGCATTTGCGATACTGCTAACTGGTGTCTGGCAAGGGGGTGCGTCTGGAGCGGCGTTGACGGTGCAGTCGTTTGAGCG
TGGGCTTCCGTTTGGGATTGGACAGTACGTGGTGGGTGTGAGCGTGCCGTTGTTTGCGTTCACGACGATGCTAGGTTGGT
TGTACTATGGGGAGCGGTGTGCGGAATTTTTGTGTGGACCGCGCGTATGTCCGCTGTACCGGGCCGTGTGGTTACCGTTT
GTGTACGTGGGTGCCGTGGGGAGTTTGAGTAGCGTGTGGAATATCTCGGATGCGTTCAATGGACTGATGGCGTTGCCGAA
CCTGGTGGGGCTGTTATTTTTGGCTCGTCAGGGGATGCGTCTACGCGAGGAGTTTTTTGCGCAGCAGGAAGGGTAG

Upstream 100 bases:

>100_bases
GCGGAGGATAGGGCGCGGGTGAAGGAAGTGCAAGGGAGGTGGTGGTCTTGACAGGATGAAATCAGGTGTGGTCGGGTGTA
GGGGCGGGGGAGAGGTAGGA

Downstream 100 bases:

>100_bases
GGGGGCGCGGCGTTGGATGTCGCACGAGAACGGCGTCGCTGTGCATGGCAATGTAGGGGTAGAAGGTGCTCGGAAGTTCT
TGGAAAGTAGGTGCGTTTTG

Product: D-alanine glycine permease

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MGVVEWVGEWMHAVVWSFPMVVLLLGTGCYVTVCMKFFPVVRLWYVLRQTIGGRGGKKGGSGEVSAFRAVSTALAATLGS
GNIVGVATAIAIGGPGAIFWIWVTGIFGMGTKFVEVVLAVYYRRQTGDGRFVGGPMYYLKDGVGVPGAGVLASLFCIFSV
IASFGIGNMTQSNSVALVFEDVFCVDVRVTGAVLMVLVGLVSVGGLKSISWVTGVMVPGMAILYVCVGVVLVCCNTRQLV
PVCWDIVSGAFAGTAAVGGFAGSVVRQAIAVGISRGVAVNEAGLGTAPIAHAAAITDHPVRQGLWGIFEVFVGTMVVSSV
TAFAILLTGVWQGGASGAALTVQSFERGLPFGIGQYVVGVSVPLFAFTTMLGWLYYGERCAEFLCGPRVCPLYRAVWLPF
VYVGAVGSLSSVWNISDAFNGLMALPNLVGLLFLARQGMRLREEFFAQQEG

Sequences:

>Translated_451_residues
MGVVEWVGEWMHAVVWSFPMVVLLLGTGCYVTVCMKFFPVVRLWYVLRQTIGGRGGKKGGSGEVSAFRAVSTALAATLGS
GNIVGVATAIAIGGPGAIFWIWVTGIFGMGTKFVEVVLAVYYRRQTGDGRFVGGPMYYLKDGVGVPGAGVLASLFCIFSV
IASFGIGNMTQSNSVALVFEDVFCVDVRVTGAVLMVLVGLVSVGGLKSISWVTGVMVPGMAILYVCVGVVLVCCNTRQLV
PVCWDIVSGAFAGTAAVGGFAGSVVRQAIAVGISRGVAVNEAGLGTAPIAHAAAITDHPVRQGLWGIFEVFVGTMVVSSV
TAFAILLTGVWQGGASGAALTVQSFERGLPFGIGQYVVGVSVPLFAFTTMLGWLYYGERCAEFLCGPRVCPLYRAVWLPF
VYVGAVGSLSSVWNISDAFNGLMALPNLVGLLFLARQGMRLREEFFAQQEG
>Mature_450_residues
GVVEWVGEWMHAVVWSFPMVVLLLGTGCYVTVCMKFFPVVRLWYVLRQTIGGRGGKKGGSGEVSAFRAVSTALAATLGSG
NIVGVATAIAIGGPGAIFWIWVTGIFGMGTKFVEVVLAVYYRRQTGDGRFVGGPMYYLKDGVGVPGAGVLASLFCIFSVI
ASFGIGNMTQSNSVALVFEDVFCVDVRVTGAVLMVLVGLVSVGGLKSISWVTGVMVPGMAILYVCVGVVLVCCNTRQLVP
VCWDIVSGAFAGTAAVGGFAGSVVRQAIAVGISRGVAVNEAGLGTAPIAHAAAITDHPVRQGLWGIFEVFVGTMVVSSVT
AFAILLTGVWQGGASGAALTVQSFERGLPFGIGQYVVGVSVPLFAFTTMLGWLYYGERCAEFLCGPRVCPLYRAVWLPFV
YVGAVGSLSSVWNISDAFNGLMALPNLVGLLFLARQGMRLREEFFAQQEG

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=450, Percent_Identity=32.6666666666667, Blast_Score=187, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 47349; Mature: 47218

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVVEWVGEWMHAVVWSFPMVVLLLGTGCYVTVCMKFFPVVRLWYVLRQTIGGRGGKKGG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SGEVSAFRAVSTALAATLGSGNIVGVATAIAIGGPGAIFWIWVTGIFGMGTKFVEVVLAV
CCCHHHHHHHHHHHHHHCCCCCEEEHHHHHEECCCCHHHHHHHHHHHHCCHHHHHHHHHH
YYRRQTGDGRFVGGPMYYLKDGVGVPGAGVLASLFCIFSVIASFGIGNMTQSNSVALVFE
HHHHCCCCCCEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
DVFCVDVRVTGAVLMVLVGLVSVGGLKSISWVTGVMVPGMAILYVCVGVVLVCCNTRQLV
CEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
PVCWDIVSGAFAGTAAVGGFAGSVVRQAIAVGISRGVAVNEAGLGTAPIAHAAAITDHPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHCCCHH
RQGLWGIFEVFVGTMVVSSVTAFAILLTGVWQGGASGAALTVQSFERGLPFGIGQYVVGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHCCCCCCHHHHHHHH
SVPLFAFTTMLGWLYYGERCAEFLCGPRVCPLYRAVWLPFVYVGAVGSLSSVWNISDAFN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
GLMALPNLVGLLFLARQGMRLREEFFAQQEG
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure 
GVVEWVGEWMHAVVWSFPMVVLLLGTGCYVTVCMKFFPVVRLWYVLRQTIGGRGGKKGG
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SGEVSAFRAVSTALAATLGSGNIVGVATAIAIGGPGAIFWIWVTGIFGMGTKFVEVVLAV
CCCHHHHHHHHHHHHHHCCCCCEEEHHHHHEECCCCHHHHHHHHHHHHCCHHHHHHHHHH
YYRRQTGDGRFVGGPMYYLKDGVGVPGAGVLASLFCIFSVIASFGIGNMTQSNSVALVFE
HHHHCCCCCCEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
DVFCVDVRVTGAVLMVLVGLVSVGGLKSISWVTGVMVPGMAILYVCVGVVLVCCNTRQLV
CEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
PVCWDIVSGAFAGTAAVGGFAGSVVRQAIAVGISRGVAVNEAGLGTAPIAHAAAITDHPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHCCCHH
RQGLWGIFEVFVGTMVVSSVTAFAILLTGVWQGGASGAALTVQSFERGLPFGIGQYVVGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHCCCCCCHHHHHHHH
SVPLFAFTTMLGWLYYGERCAEFLCGPRVCPLYRAVWLPFVYVGAVGSLSSVWNISDAFN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
GLMALPNLVGLLFLARQGMRLREEFFAQQEG
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]