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The map label for this gene is fliF

Identifier: 189025632

GI number: 189025632

Start: 424444

End: 426147

Strand: Direct

Name: fliF

Synonym: TPASS_0399

Alternate gene names: 189025632

Gene position: 424444-426147 (Clockwise)

Preceding gene: 189025631

Following gene: 189025633

Centisome position: 37.25

GC content: 50.12

Gene sequence:

>1704_bases
GTGGGCGAATGGTTGGGGCAGCTCGGAGTCAAACTCAAAACACAGTGGAAGAAGTGGACGCTCGTGCAGAAGTCTGTGCT
TGCCGGCGCGGCGCTCGTGTCTGTCATGGGGGTTGTTGTCTTGCTCACGTGGTCGGCGAAGCCGACGCTCGTGCCACTTA
TCGACACTCCTATCACTGATGAGACGGTGCGGGAAAAGATTATCCTGCGCCTTAACGAAGAGAATGTGCGTGCAACCGTC
TCAAGCGTTGGGTTGATTTCTGTCTCGGATGAGAAGACAGCGCGTCGTATGCGCAGCATCTTAATTCGCGAAGATTTGAT
CCCAAAAAATGTGGACCCATGGGCCATATTCGACGTCGAGCGATGGACGCGTACTGACTTTGAGCGCAGGGTGGACGTGC
GGCGTGCAATTAATAATACCGTTACCAATCATATCAAAGCGCTCGACGACATCGATGATGCCCATGTAGTAATAAACGTG
CCTGAGGATGCGCTTTTTCAGGCAGACCAGAAACCTATTACTGCGAGCGTTGTCATTTTCCCTAAACCGTCGAGCACGAT
CGCCTCAGAAAGAAAAAAAATAGAAGGCATTCAGAAACTATTAAAGCTTGCAGTTCCTGGACTGAAGGATGAAAACATCA
CGATTGTAGATAGTGATGCTACCGTCCTAAATGATTTTGAAGGGTTCAAGGACGCTGATCGGCTGAGTCTCATTGAAAAG
CAACAGAAAATGATTGCGAAGCTGGAATCCCAGTATGAGGCAAAAGTGCTGGCTCTCTTGCAAAAGACGTACGGTAAAGA
CCGGGTGCGCGACTTAAATATCAAAATTGAAATGGATCTTTCTGAAAAGACGTCGCAGACTACCAAGTATCTGCCTATAG
AAATCCGTCAGGACAATCCGGATACCCCGTGGGATGATTCTCAGGTTGTGCCCTCTGTCACTTCGATATCTGAAACGGCA
ACCACTACGTGGCAGGGTACGGGGCTTAACCCTGAAGGACCGCCGGGAGTTGAGGGTCAAACACCTCCTGCATACAAAGA
CATGAGCAACCAGGTGGGACTTTCTAACCAGTCGGTCGTTAAGAAGCAAGAGGCGATTAGCAAGAGTGAGATCAACGAAG
TAGTGAGCCCGGTGCTCGGCCGCAGGACGGTGTCGGTCAATATCGATGGAGAATGGCGCAAAAAGAGAGACGAGCACGGA
AGATTCATTGTGAAGGAAGGACACATTGAACGTGAGTATATCCCCATCTCTGCTGAGGAGCTGCGGGAGGCAACGAAGGC
AGTGCAGGATGCAATCGGCTTTGATGCGGGGCGTAAGGATTCGGTAAGTGTTTTAAATATCAAATTTGACCGGACGTCAG
AATTTGATAGAGAAGATGAGCATTACCTGCGCGTCCAGCAGAGGAACATGATCATCTTATACTCCCTTGCCAGTGTGGCA
ATCGTTTTATTTATCTTCATGGTATACAAGGTTATCAGCAAAGAGGTGGAGCGTCGCCGTCGTCTGCGGGAAGAGGAGCT
TTTAAGGCAGCAGCAACTGATGAGGGAGCGTGCCCTGTGGGAGGCTGAACAGGCGGGGATGAATGTTTCCATGTCGGTGG
AAGAGCGTAAGCGGCTTGAATTGCAAGAGAATGTGTTGAATATGGCGCGGGAGCATCCGGAAGATGTTGCGTTGCTTGTG
AGAACGTGGTTGATGGAGGAGTAG

Upstream 100 bases:

>100_bases
CTGTTCTGCAAAAAGAGTACCGACGGTATATCAGGTGAAAAGAGGGTGGGACGCGCTTAGTGCGCATTGGCTCGTTCTAT
AGTGAGGGGAGGGGACACGC

Downstream 100 bases:

>100_bases
TACTATGGCCGTTACATCCGTGAAGGATAAGCTCGCCACGGGAGAAAAAAAGCAACGGGATATCAAGTCTCTCAATGGTC
GGCAAAAGGCAGCGATATTT

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MGEWLGQLGVKLKTQWKKWTLVQKSVLAGAALVSVMGVVVLLTWSAKPTLVPLIDTPITDETVREKIILRLNEENVRATV
SSVGLISVSDEKTARRMRSILIREDLIPKNVDPWAIFDVERWTRTDFERRVDVRRAINNTVTNHIKALDDIDDAHVVINV
PEDALFQADQKPITASVVIFPKPSSTIASERKKIEGIQKLLKLAVPGLKDENITIVDSDATVLNDFEGFKDADRLSLIEK
QQKMIAKLESQYEAKVLALLQKTYGKDRVRDLNIKIEMDLSEKTSQTTKYLPIEIRQDNPDTPWDDSQVVPSVTSISETA
TTTWQGTGLNPEGPPGVEGQTPPAYKDMSNQVGLSNQSVVKKQEAISKSEINEVVSPVLGRRTVSVNIDGEWRKKRDEHG
RFIVKEGHIEREYIPISAEELREATKAVQDAIGFDAGRKDSVSVLNIKFDRTSEFDREDEHYLRVQQRNMIILYSLASVA
IVLFIFMVYKVISKEVERRRRLREEELLRQQQLMRERALWEAEQAGMNVSMSVEERKRLELQENVLNMAREHPEDVALLV
RTWLMEE

Sequences:

>Translated_567_residues
MGEWLGQLGVKLKTQWKKWTLVQKSVLAGAALVSVMGVVVLLTWSAKPTLVPLIDTPITDETVREKIILRLNEENVRATV
SSVGLISVSDEKTARRMRSILIREDLIPKNVDPWAIFDVERWTRTDFERRVDVRRAINNTVTNHIKALDDIDDAHVVINV
PEDALFQADQKPITASVVIFPKPSSTIASERKKIEGIQKLLKLAVPGLKDENITIVDSDATVLNDFEGFKDADRLSLIEK
QQKMIAKLESQYEAKVLALLQKTYGKDRVRDLNIKIEMDLSEKTSQTTKYLPIEIRQDNPDTPWDDSQVVPSVTSISETA
TTTWQGTGLNPEGPPGVEGQTPPAYKDMSNQVGLSNQSVVKKQEAISKSEINEVVSPVLGRRTVSVNIDGEWRKKRDEHG
RFIVKEGHIEREYIPISAEELREATKAVQDAIGFDAGRKDSVSVLNIKFDRTSEFDREDEHYLRVQQRNMIILYSLASVA
IVLFIFMVYKVISKEVERRRRLREEELLRQQQLMRERALWEAEQAGMNVSMSVEERKRLELQENVLNMAREHPEDVALLV
RTWLMEE
>Mature_566_residues
GEWLGQLGVKLKTQWKKWTLVQKSVLAGAALVSVMGVVVLLTWSAKPTLVPLIDTPITDETVREKIILRLNEENVRATVS
SVGLISVSDEKTARRMRSILIREDLIPKNVDPWAIFDVERWTRTDFERRVDVRRAINNTVTNHIKALDDIDDAHVVINVP
EDALFQADQKPITASVVIFPKPSSTIASERKKIEGIQKLLKLAVPGLKDENITIVDSDATVLNDFEGFKDADRLSLIEKQ
QKMIAKLESQYEAKVLALLQKTYGKDRVRDLNIKIEMDLSEKTSQTTKYLPIEIRQDNPDTPWDDSQVVPSVTSISETAT
TTWQGTGLNPEGPPGVEGQTPPAYKDMSNQVGLSNQSVVKKQEAISKSEINEVVSPVLGRRTVSVNIDGEWRKKRDEHGR
FIVKEGHIEREYIPISAEELREATKAVQDAIGFDAGRKDSVSVLNIKFDRTSEFDREDEHYLRVQQRNMIILYSLASVAI
VLFIFMVYKVISKEVERRRRLREEELLRQQQLMRERALWEAEQAGMNVSMSVEERKRLELQENVLNMAREHPEDVALLVR
TWLMEE

Specific function: The M ring may be actively involved in energy transduction

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family

Homologues:

Organism=Escherichia coli, GI1788248, Length=568, Percent_Identity=22.5352112676056, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): FLIF_TREPA (O83414)

Other databases:

- EMBL:   AE000520
- PIR:   F71328
- RefSeq:   NP_218839.1
- ProteinModelPortal:   O83414
- IntAct:   O83414
- MINT:   MINT-6479275
- GeneID:   2610804
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0399
- NMPDR:   fig|243276.1.peg.398
- TIGR:   TP_0399
- HOGENOM:   HBG352882
- OMA:   FAFVIMR
- ProtClustDB:   PRK06007
- BioCyc:   TPAL243276:TP_0399-MONOMER
- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182
- PRINTS:   PR01009
- TIGRFAMs:   TIGR00206

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C

EC number: NA

Molecular weight: Translated: 64640; Mature: 64509

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1e1b3cec)-; HASH(0x202d24b8)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEWLGQLGVKLKTQWKKWTLVQKSVLAGAALVSVMGVVVLLTWSAKPTLVPLIDTPITD
CCCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCH
ETVREKIILRLNEENVRATVSSVGLISVSDEKTARRMRSILIREDLIPKNVDPWAIFDVE
HHHHHHHEEEECCCHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEECH
RWTRTDFERRVDVRRAINNTVTNHIKALDDIDDAHVVINVPEDALFQADQKPITASVVIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCCEEEEEEE
PKPSSTIASERKKIEGIQKLLKLAVPGLKDENITIVDSDATVLNDFEGFKDADRLSLIEK
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCCCCHHHHHHHHH
QQKMIAKLESQYEAKVLALLQKTYGKDRVRDLNIKIEMDLSEKTSQTTKYLPIEIRQDNP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCEEEEEEEECCCC
DTPWDDSQVVPSVTSISETATTTWQGTGLNPEGPPGVEGQTPPAYKDMSNQVGLSNQSVV
CCCCCCCCCCCCHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHH
KKQEAISKSEINEVVSPVLGRRTVSVNIDGEWRKKRDEHGRFIVKEGHIEREYIPISAEE
HHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHCHHCCCCEEEECCCCCCEEECCCHHH
LREATKAVQDAIGFDAGRKDSVSVLNIKFDRTSEFDREDEHYLRVQQRNMIILYSLASVA
HHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHEECCCCEEEEEEHHHHH
IVLFIFMVYKVISKEVERRRRLREEELLRQQQLMRERALWEAEQAGMNVSMSVEERKRLE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
LQENVLNMAREHPEDVALLVRTWLMEE
HHHHHHHHHHHCHHHHHHHHHHHHCCH
>Mature Secondary Structure 
GEWLGQLGVKLKTQWKKWTLVQKSVLAGAALVSVMGVVVLLTWSAKPTLVPLIDTPITD
CCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEECCCCCH
ETVREKIILRLNEENVRATVSSVGLISVSDEKTARRMRSILIREDLIPKNVDPWAIFDVE
HHHHHHHEEEECCCHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEECH
RWTRTDFERRVDVRRAINNTVTNHIKALDDIDDAHVVINVPEDALFQADQKPITASVVIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCCCCEEEEEEE
PKPSSTIASERKKIEGIQKLLKLAVPGLKDENITIVDSDATVLNDFEGFKDADRLSLIEK
ECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCCCCHHHHHHHHH
QQKMIAKLESQYEAKVLALLQKTYGKDRVRDLNIKIEMDLSEKTSQTTKYLPIEIRQDNP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCEEEEEEEECCCC
DTPWDDSQVVPSVTSISETATTTWQGTGLNPEGPPGVEGQTPPAYKDMSNQVGLSNQSVV
CCCCCCCCCCCCHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHH
KKQEAISKSEINEVVSPVLGRRTVSVNIDGEWRKKRDEHGRFIVKEGHIEREYIPISAEE
HHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHCHHCCCCEEEECCCCCCEEECCCHHH
LREATKAVQDAIGFDAGRKDSVSVLNIKFDRTSEFDREDEHYLRVQQRNMIILYSLASVA
HHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHEECCCCEEEEEEHHHHH
IVLFIFMVYKVISKEVERRRRLREEELLRQQQLMRERALWEAEQAGMNVSMSVEERKRLE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHH
LQENVLNMAREHPEDVALLVRTWLMEE
HHHHHHHHHHHCHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9665876