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The map label for this gene is birA [H]

Identifier: 189025590

GI number: 189025590

Start: 380750

End: 381490

Strand: Direct

Name: birA [H]

Synonym: TPASS_0357

Alternate gene names: 189025590

Gene position: 380750-381490 (Clockwise)

Preceding gene: 189025589

Following gene: 189025593

Centisome position: 33.42

GC content: 58.84

Gene sequence:

>741_bases
GTGTGCGTCGCGGTGTGTTTGGTTCCTGTTAGGAACCCCTTCGGGGCTTCTGTCTATTTTGCTCCCAAGACTGCTAGTAC
TATGGATGAGGCTGCGTCTCGCGCCCAGGGTTGCGAGGAGGGTGCCGTCTTTTGCGCTGGTGAGCAGGTTGCTGGACGTG
GTCGTGGTGACCAGAGAAAGTGGCAATCGGAGCCGGGAAAGAATCTTTTGTGTACTGTCGTTTTGCGGCGTGTTGCTTTC
CCTGCGTTTTCGCTCTGTGTTGGATATGCGGTCGCTCTTGCATACATGGCCTTCCTCGGAGGTGTGTGCGCACCGCGCAT
TAAGTGGCCCAACGACGTCCTGGTGTGCGATCGTAAGATCGCCGGGGTGCTCTGCCAGGTGCGCGCGGGAGCGCTTCTCG
TGGGTATTGGGTGTAACCTCCTGCAGGTGAAGTTTCCGCCCGAGTTGTCGCATGCCTGCTCTCTCGCGCAGATTGTCGGA
GGCGAGCGGTGCCCTGATCCGTTTGCTTTCCTTCCTGTGCTGTTGGATCGGCTGTATGCCTGTGTCATGGCGCCGCCGAG
TATCGGCGTGCTTGAGTCGTGTCTGTGGAAGCAGGGTGAGTACGTCTGTTTCCGCGAATGTGCGGGAACACGTCCTCCGA
TTCTGGGTCGTGTTGTCGGATTGGCCGCGGACGGCGCGCTGTGCATAGATAGCGCTGGGTCGATGCGATCGTATTACTCT
GGGGAGATCGCCTTTTGGTGA

Upstream 100 bases:

>100_bases
GGAGGATGGGGAGGACTTTCCATCGTGGCGCATGTTTTTGGCGTAAGGTGCTTTCGCGTGCGTTATCTCATTTCTCGTCG
TCTTTTGGTTCTCCCCGTTT

Downstream 100 bases:

>100_bases
GGTGTCATTTCTTTTTACTGAAAAAGCGAAAGTTAATTTCAGAGAACAGTGGTAGCTCGCGTTCGCGCCGCATTAGCCAC
TGTGCACTTACCTCTCCGGA

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MCVAVCLVPVRNPFGASVYFAPKTASTMDEAASRAQGCEEGAVFCAGEQVAGRGRGDQRKWQSEPGKNLLCTVVLRRVAF
PAFSLCVGYAVALAYMAFLGGVCAPRIKWPNDVLVCDRKIAGVLCQVRAGALLVGIGCNLLQVKFPPELSHACSLAQIVG
GERCPDPFAFLPVLLDRLYACVMAPPSIGVLESCLWKQGEYVCFRECAGTRPPILGRVVGLAADGALCIDSAGSMRSYYS
GEIAFW

Sequences:

>Translated_246_residues
MCVAVCLVPVRNPFGASVYFAPKTASTMDEAASRAQGCEEGAVFCAGEQVAGRGRGDQRKWQSEPGKNLLCTVVLRRVAF
PAFSLCVGYAVALAYMAFLGGVCAPRIKWPNDVLVCDRKIAGVLCQVRAGALLVGIGCNLLQVKFPPELSHACSLAQIVG
GERCPDPFAFLPVLLDRLYACVMAPPSIGVLESCLWKQGEYVCFRECAGTRPPILGRVVGLAADGALCIDSAGSMRSYYS
GEIAFW
>Mature_246_residues
MCVAVCLVPVRNPFGASVYFAPKTASTMDEAASRAQGCEEGAVFCAGEQVAGRGRGDQRKWQSEPGKNLLCTVVLRRVAF
PAFSLCVGYAVALAYMAFLGGVCAPRIKWPNDVLVCDRKIAGVLCQVRAGALLVGIGCNLLQVKFPPELSHACSLAQIVG
GERCPDPFAFLPVLLDRLYACVMAPPSIGVLESCLWKQGEYVCFRECAGTRPPILGRVVGLAADGALCIDSAGSMRSYYS
GEIAFW

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 26208; Mature: 26208

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
6.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVAVCLVPVRNPFGASVYFAPKTASTMDEAASRAQGCEEGAVFCAGEQVAGRGRGDQRK
CEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEECCHHCCCCCCCCHH
WQSEPGKNLLCTVVLRRVAFPAFSLCVGYAVALAYMAFLGGVCAPRIKWPNDVLVCDRKI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
AGVLCQVRAGALLVGIGCNLLQVKFPPELSHACSLAQIVGGERCPDPFAFLPVLLDRLYA
HHHHHHHHCCEEEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
CVMAPPSIGVLESCLWKQGEYVCFRECAGTRPPILGRVVGLAADGALCIDSAGSMRSYYS
HHCCCCCHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHHHCCCCCEEECCCCCHHHHHC
GEIAFW
CCEECC
>Mature Secondary Structure
MCVAVCLVPVRNPFGASVYFAPKTASTMDEAASRAQGCEEGAVFCAGEQVAGRGRGDQRK
CEEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEECCHHCCCCCCCCHH
WQSEPGKNLLCTVVLRRVAFPAFSLCVGYAVALAYMAFLGGVCAPRIKWPNDVLVCDRKI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
AGVLCQVRAGALLVGIGCNLLQVKFPPELSHACSLAQIVGGERCPDPFAFLPVLLDRLYA
HHHHHHHHCCEEEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
CVMAPPSIGVLESCLWKQGEYVCFRECAGTRPPILGRVVGLAADGALCIDSAGSMRSYYS
HHCCCCCHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHHHCCCCCEEECCCCCHHHHHC
GEIAFW
CCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]