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The map label for this gene is rnhA

Identifier: 189025586

GI number: 189025586

Start: 378378

End: 378887

Strand: Reverse

Name: rnhA

Synonym: TPASS_0353

Alternate gene names: 189025586

Gene position: 378887-378378 (Counterclockwise)

Preceding gene: 189025588

Following gene: 189025582

Centisome position: 33.25

GC content: 60.24

Gene sequence:

>510_bases
ATGAACGCGCACGCTGCACTTACCCTGTACACTGACGGCGCCTGTCTGGGAAACCCGGGTCCGGGCGGGTGGGCGTTTGC
TCTGGTGCCCTCAGACGTACCCTTTCTAGAAACCGGACAAASSGCACCAGAAGCTGCCGCATTCACTAGAAGCGGCAGCG
CCTACCCGAGCACAAACAATCGCATGGAACTGTGCGCGGTCATCAACGCACTGCAAGAGGCACACGGACGCGCTGCAGAG
GCGGTGGTAGTGGTCACCGACTCCCAATACGTACGCAAAGGCATCACCCAGTGGATCCATACCTGGAAACACAACGGCTG
GAAAACGGCTGCCAAACAACCGGTCAAGAACAAGGATTTGTGGGAAGCGCTCAGCGCACTCGCCGACGCCCTATCCGTCG
AGTGGCGCTGGGTAAAAGGACATGCAGGAGACCCGTACAACGAACTGTGCGACCGGCTAGCCACCGACGCTGCGCGCCGC
GCTGCACAAAGCACTGCAGACTGTCCCTAG

Upstream 100 bases:

>100_bases
GCCTCATTCTCCCTCCTTGCGAAAATATCTTCGCTCCATCACCAACTGTCGGACCAATACATTGTCCCCACGGCGCAAAA
CTGTTACCGTAGGCGCGACT

Downstream 100 bases:

>100_bases
TACGGGTCCAGGGACATACTGTTTAGTAAATCGCGCAGGGCTACAATCTCTGCACGCGTAAGCGTTACCCCCTTTCCCAT
CTTGCTCTTGTCTGGGGACC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MNAHAALTLYTDGACLGNPGPGGWAFALVPSDVPFLETGQXAPEAAAFTRSGSAYPSTNNRMELCAVINALQEAHGRAAE
AVVVVTDSQYVRKGITQWIHTWKHNGWKTAAKQPVKNKDLWEALSALADALSVEWRWVKGHAGDPYNELCDRLATDAARR
AAQSTADCP

Sequences:

>Translated_169_residues
MNAHAALTLYTDGACLGNPGPGGWAFALVPSDVPFLETGQXAPEAAAFTRSGSAYPSTNNRMELCAVINALQEAHGRAAE
AVVVVTDSQYVRKGITQWIHTWKHNGWKTAAKQPVKNKDLWEALSALADALSVEWRWVKGHAGDPYNELCDRLATDAARR
AAQSTADCP
>Mature_169_residues
MNAHAALTLYTDGACLGNPGPGGWAFALVPSDVPFLETGQXAPEAAAFTRSGSAYPSTNNRMELCAVINALQEAHGRAAE
AVVVVTDSQYVRKGITQWIHTWKHNGWKTAAKQPVKNKDLWEALSALADALSVEWRWVKGHAGDPYNELCDRLATDAARR
AAQSTADCP

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=165, Percent_Identity=31.5151515151515, Blast_Score=87, Evalue=9e-18,
Organism=Escherichia coli, GI1786408, Length=152, Percent_Identity=52.6315789473684, Blast_Score=145, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI115532542, Length=157, Percent_Identity=34.3949044585987, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17505482, Length=145, Percent_Identity=35.8620689655172, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI17137476, Length=156, Percent_Identity=33.974358974359, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45552509, Length=156, Percent_Identity=33.974358974359, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_TREPA (O83372)

Other databases:

- EMBL:   AE000520
- PIR:   G71333
- RefSeq:   NP_218793.1
- ProteinModelPortal:   O83372
- GeneID:   2611470
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0353
- NMPDR:   fig|243276.1.peg.352
- TIGR:   TP_0353
- HOGENOM:   HBG742437
- OMA:   WATILRY
- ProtClustDB:   CLSK2300196
- BioCyc:   TPAL243276:TP_0353-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 18131; Mature: 18131

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAHAALTLYTDGACLGNPGPGGWAFALVPSDVPFLETGQXAPEAAAFTRSGSAYPSTNN
CCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
RMELCAVINALQEAHGRAAEAVVVVTDSQYVRKGITQWIHTWKHNGWKTAAKQPVKNKDL
HHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHH
WEALSALADALSVEWRWVKGHAGDPYNELCDRLATDAARRAAQSTADCP
HHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNAHAALTLYTDGACLGNPGPGGWAFALVPSDVPFLETGQXAPEAAAFTRSGSAYPSTNN
CCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
RMELCAVINALQEAHGRAAEAVVVVTDSQYVRKGITQWIHTWKHNGWKTAAKQPVKNKDL
HHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHH
WEALSALADALSVEWRWVKGHAGDPYNELCDRLATDAARRAAQSTADCP
HHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876