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The map label for this gene is folC [H]

Identifier: 189025573

GI number: 189025573

Start: 363190

End: 364662

Strand: Reverse

Name: folC [H]

Synonym: TPASS_0340

Alternate gene names: 189025573

Gene position: 364662-363190 (Counterclockwise)

Preceding gene: 189025577

Following gene: 189025568

Centisome position: 32.0

GC content: 46.91

Gene sequence:

>1473_bases
ATGTCAGACCTTAGCAATGTACATCAGTTCTCTTTCCCGGCAAGGATTGCTGCCTCTTCCGTACCGCATATTATAGTGCG
ACCGAGCGCACACGGAGTTTCACATATACCGGTGCATTCTTTTCAAGATTTTTACGCCTGGGTTGAGCGCATTCGCACGC
TTGAGAAAATGCCCCCAAGCGGCACACCACCTCTGCACAGGATGACTGCACTTGCACGCGCGCTTTGTAATCCACACAAC
GCATATCAGAGCATCCATGTTGCGGGCACGAAGGGAAAAGGTTCCAGCGCACGTATGCTTGCAGCGATACTCAATGAAAT
TGGATTCAACACCGGGCTATACGCTTCTCCTCACGTTATGGATCCGAGAGAGAGAATAACACGCGCGGGTGTGTTTTTTT
CACCTGCCGAGTATGCAAGCGCGTGCACACACGTATACCACACGGTGAAAAAAACAGAGAATCTGCGCGACTACGGCCAG
GCGACGTGGTTTGAGCTTATAACGCTACTGGCGTTCATGCTATTTGCACAACAACGCATGGAATGGTCCGTTTTTGAAGT
AGGACTTGGAGGAAGACTAGATGCAACAAACATCATTTGTCCTAGTATCTGTCTCCTTCTCCCCATAGAACAAGAACACA
CGCGCATATTAGGAACACGTATAAAAAGTATTGCAAAAGAAAAGGGCGGCATTATCAAACCCTATACGCCTATTTTTTGT
TTTGATCAGCCAGAAGATGCATTGCACGTTTTTAAACATATCGCGCGAGAAAAACATGCTCCCTTCTTTTACTTACCAGA
TATGGTCACACATATTGAAAGTTCAATAAAACACTTCACACACACTGCAACTCTCAGTTTTAACGCCGCGCATCCTGTTG
GCAGATTATTCGCTCGAAATATACACTGCACACTCGCGTTATGCGATATTGTTCAGGCAAAAAATGCAGCACTGGCTGCG
TGCGCGGCAAAATATTTATTTCCTACCGTGAGCGAAACGTTAATCGAGCGGGGACTGTCCCGTGCATACGTGCCAGCACG
CTTTGAAATTATGCAGGAAGATCCACTCATAGTGATTGACGGTGCGCACACTACGCAGAGTATACGGTGCGCATGTCGTA
CGTTCTCTTCTTTACTACCTTCTGCACAATACATTTTACTCTTTGCGTGCGCGGCGGACAAAGCAGTGTCCCAGTTCCCC
CCTCTTTTTTCACATGCCGCCAGCGAAATTTTTTTAACCATACCGGGCACATCTAAACATGCAGATATACAAAAAACATA
CCACGCGTTTCGTACGACATTTCACCAAACAGTCCCAATTTTTTGGAGCGAAGACTTTGCGTCAATTATTTCCCGTGCAC
TGTATGCCAGTTTTGCGCAACGTAAAGCGCTCCTTGCCATCGGTTCTTTCTATTTAGCTGCTGAAGTAAAACGCATATAC
GCACGCACCTGCCACACCCTACCTAACCGATAA

Upstream 100 bases:

>100_bases
GCGCAGACATGCCAGTGCCCTTGATACCAATCATGTGAATCTTGATACCTGACACATCTTTTGGTAGAGTCCCCCGCTTC
ATGGGCGGATTTTAGCTCAA

Downstream 100 bases:

>100_bases
TCGGTGTATCAGTCGAACAAAACGAAGCGGTATGGGCGCTACCAGCGTTATGCGTTTTTTCGTTCTCGGAAGAACAAACC
GTACTGCGTACGCATGAAGC

Product: folylpolyglutamate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSDLSNVHQFSFPARIAASSVPHIIVRPSAHGVSHIPVHSFQDFYAWVERIRTLEKMPPSGTPPLHRMTALARALCNPHN
AYQSIHVAGTKGKGSSARMLAAILNEIGFNTGLYASPHVMDPRERITRAGVFFSPAEYASACTHVYHTVKKTENLRDYGQ
ATWFELITLLAFMLFAQQRMEWSVFEVGLGGRLDATNIICPSICLLLPIEQEHTRILGTRIKSIAKEKGGIIKPYTPIFC
FDQPEDALHVFKHIAREKHAPFFYLPDMVTHIESSIKHFTHTATLSFNAAHPVGRLFARNIHCTLALCDIVQAKNAALAA
CAAKYLFPTVSETLIERGLSRAYVPARFEIMQEDPLIVIDGAHTTQSIRCACRTFSSLLPSAQYILLFACAADKAVSQFP
PLFSHAASEIFLTIPGTSKHADIQKTYHAFRTTFHQTVPIFWSEDFASIISRALYASFAQRKALLAIGSFYLAAEVKRIY
ARTCHTLPNR

Sequences:

>Translated_490_residues
MSDLSNVHQFSFPARIAASSVPHIIVRPSAHGVSHIPVHSFQDFYAWVERIRTLEKMPPSGTPPLHRMTALARALCNPHN
AYQSIHVAGTKGKGSSARMLAAILNEIGFNTGLYASPHVMDPRERITRAGVFFSPAEYASACTHVYHTVKKTENLRDYGQ
ATWFELITLLAFMLFAQQRMEWSVFEVGLGGRLDATNIICPSICLLLPIEQEHTRILGTRIKSIAKEKGGIIKPYTPIFC
FDQPEDALHVFKHIAREKHAPFFYLPDMVTHIESSIKHFTHTATLSFNAAHPVGRLFARNIHCTLALCDIVQAKNAALAA
CAAKYLFPTVSETLIERGLSRAYVPARFEIMQEDPLIVIDGAHTTQSIRCACRTFSSLLPSAQYILLFACAADKAVSQFP
PLFSHAASEIFLTIPGTSKHADIQKTYHAFRTTFHQTVPIFWSEDFASIISRALYASFAQRKALLAIGSFYLAAEVKRIY
ARTCHTLPNR
>Mature_489_residues
SDLSNVHQFSFPARIAASSVPHIIVRPSAHGVSHIPVHSFQDFYAWVERIRTLEKMPPSGTPPLHRMTALARALCNPHNA
YQSIHVAGTKGKGSSARMLAAILNEIGFNTGLYASPHVMDPRERITRAGVFFSPAEYASACTHVYHTVKKTENLRDYGQA
TWFELITLLAFMLFAQQRMEWSVFEVGLGGRLDATNIICPSICLLLPIEQEHTRILGTRIKSIAKEKGGIIKPYTPIFCF
DQPEDALHVFKHIAREKHAPFFYLPDMVTHIESSIKHFTHTATLSFNAAHPVGRLFARNIHCTLALCDIVQAKNAALAAC
AAKYLFPTVSETLIERGLSRAYVPARFEIMQEDPLIVIDGAHTTQSIRCACRTFSSLLPSAQYILLFACAADKAVSQFPP
LFSHAASEIFLTIPGTSKHADIQKTYHAFRTTFHQTVPIFWSEDFASIISRALYASFAQRKALLAIGSFYLAAEVKRIYA
RTCHTLPNR

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=375, Percent_Identity=30.1333333333333, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI66932984, Length=375, Percent_Identity=30.1333333333333, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1788654, Length=311, Percent_Identity=30.5466237942122, Blast_Score=116, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17553150, Length=364, Percent_Identity=29.6703296703297, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17553148, Length=364, Percent_Identity=29.6703296703297, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71984923, Length=364, Percent_Identity=29.6703296703297, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324815, Length=406, Percent_Identity=28.0788177339901, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323760, Length=431, Percent_Identity=25.0580046403712, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24641571, Length=329, Percent_Identity=28.2674772036474, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24581568, Length=298, Percent_Identity=26.1744966442953, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 54653; Mature: 54522

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLSNVHQFSFPARIAASSVPHIIVRPSAHGVSHIPVHSFQDFYAWVERIRTLEKMPPS
CCCHHHHHHHCCCHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GTPPLHRMTALARALCNPHNAYQSIHVAGTKGKGSSARMLAAILNEIGFNTGLYASPHVM
CCCHHHHHHHHHHHHCCCHHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
DPRERITRAGVFFSPAEYASACTHVYHTVKKTENLRDYGQATWFELITLLAFMLFAQQRM
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EWSVFEVGLGGRLDATNIICPSICLLLPIEQEHTRILGTRIKSIAKEKGGIIKPYTPIFC
CCEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCEEE
FDQPEDALHVFKHIAREKHAPFFYLPDMVTHIESSIKHFTHTATLSFNAAHPVGRLFARN
ECCCHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHCC
IHCTLALCDIVQAKNAALAACAAKYLFPTVSETLIERGLSRAYVPARFEIMQEDPLIVID
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHEEECCCCEEEEE
GAHTTQSIRCACRTFSSLLPSAQYILLFACAADKAVSQFPPLFSHAASEIFLTIPGTSKH
CCCCHHHHHHHHHHHHHHCCCHHHHHHEEHHHHHHHHHCCHHHHHCCCEEEEECCCCCCC
ADIQKTYHAFRTTFHQTVPIFWSEDFASIISRALYASFAQRKALLAIGSFYLAAEVKRIY
HHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARTCHTLPNR
HHHHHCCCCC
>Mature Secondary Structure 
SDLSNVHQFSFPARIAASSVPHIIVRPSAHGVSHIPVHSFQDFYAWVERIRTLEKMPPS
CCHHHHHHHCCCHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GTPPLHRMTALARALCNPHNAYQSIHVAGTKGKGSSARMLAAILNEIGFNTGLYASPHVM
CCCHHHHHHHHHHHHCCCHHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
DPRERITRAGVFFSPAEYASACTHVYHTVKKTENLRDYGQATWFELITLLAFMLFAQQRM
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EWSVFEVGLGGRLDATNIICPSICLLLPIEQEHTRILGTRIKSIAKEKGGIIKPYTPIFC
CCEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCEEE
FDQPEDALHVFKHIAREKHAPFFYLPDMVTHIESSIKHFTHTATLSFNAAHPVGRLFARN
ECCCHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHCC
IHCTLALCDIVQAKNAALAACAAKYLFPTVSETLIERGLSRAYVPARFEIMQEDPLIVID
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHEEECCCCEEEEE
GAHTTQSIRCACRTFSSLLPSAQYILLFACAADKAVSQFPPLFSHAASEIFLTIPGTSKH
CCCCHHHHHHHHHHHHHHCCCHHHHHHEEHHHHHHHHHCCHHHHHCCCEEEEECCCCCCC
ADIQKTYHAFRTTFHQTVPIFWSEDFASIISRALYASFAQRKALLAIGSFYLAAEVKRIY
HHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARTCHTLPNR
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]