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The map label for this gene is ksgA

Identifier: 189025570

GI number: 189025570

Start: 360834

End: 361691

Strand: Direct

Name: ksgA

Synonym: TPASS_0337

Alternate gene names: 189025570

Gene position: 360834-361691 (Clockwise)

Preceding gene: 189025569

Following gene: 189025571

Centisome position: 31.67

GC content: 48.02

Gene sequence:

>858_bases
ATGTGTTGCCCGAATTATAATTCGGCGCGTGCACTTGCACAGTTTTTGACGGAGCGCGGTTTGCGGATGCATAAAAAGTG
GGGGCAGAATTTTCTGCTCGATCCGGTGTTACGTACGCAGCTTGTTAAGATATTGGCGCCGGAGCGTGGGGAACGTGTAT
GGGAAATTGGTGCAGGCATTGGTGCGATGACCGCACTTTTGGTGCAAAACAGTGATTTTTTAACAGTGTTTGAAATTGAT
CGCGGCTTTGTGCAGACATTGCGCAAACTTTTTGATGCACACGTCCGTGTGATAGAAGGGGATGTGTTGCAACAGTGGCA
TGCTGCAGCAGCACAGGAACAACCTGCGTGTGTTCTAGGAAATTTACCCTACAATATTGCTGCCCGTTTTATTGGAAACA
CGATCGAATCAGGCTATATTTTTAAGCGTATGGTGGTGACCGTTCAAAAAGAAATCGGGTTGAGAATGACTGCGCTCCCT
GCACAAAAATGGTATTCATACTTTTCAGTACTCTGTCAGTGGCAGTATGAAGTGCGTGTGATTCGTAACGTTGCGCCTGT
CTGTTTTTGGCCGCGTCCTCATGTAGTTTCTCAAGCATTGGTACTCACCAAGCGTAATGCGGTGCCTTCTTGTGTGGATC
CTGCGCTTTTTCTGCACGTGACGAAAACTTTGTTTTCTGCGCGGCGTAAAACGGTAAGAAATAATTTACTCACGTGGCAA
AAAAGGATGCCAGGCGGTGCAGCTGTGTGTGTAGAAGAACTCTGCGCACGTGCAGGTATTGACGCGCGTGCGCGTGCAGA
GCAACTGAGCATCTATGATTTTATTACGCTTTCTGATACGCTGCGCGCGCTACTGTAG

Upstream 100 bases:

>100_bases
CAGAGTGCCTGCGGACATGTGTGTGCTGCATTATTTTCTTTTTTACTCGGTGGGGGACTACTAGAGGCGGCGCGTCGCGT
GCGTGTTCACAAGGATACAT

Downstream 100 bases:

>100_bases
TCCGGTGTGGGTGTTGAATGGCGCGTGTCTATATTCTTTTTTTCAGTGTGTTTTTTGTTTTTCCGCTCTTTTCTGAAGAC
GCCGCGCGCGATGTGGAACC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MCCPNYNSARALAQFLTERGLRMHKKWGQNFLLDPVLRTQLVKILAPERGERVWEIGAGIGAMTALLVQNSDFLTVFEID
RGFVQTLRKLFDAHVRVIEGDVLQQWHAAAAQEQPACVLGNLPYNIAARFIGNTIESGYIFKRMVVTVQKEIGLRMTALP
AQKWYSYFSVLCQWQYEVRVIRNVAPVCFWPRPHVVSQALVLTKRNAVPSCVDPALFLHVTKTLFSARRKTVRNNLLTWQ
KRMPGGAAVCVEELCARAGIDARARAEQLSIYDFITLSDTLRALL

Sequences:

>Translated_285_residues
MCCPNYNSARALAQFLTERGLRMHKKWGQNFLLDPVLRTQLVKILAPERGERVWEIGAGIGAMTALLVQNSDFLTVFEID
RGFVQTLRKLFDAHVRVIEGDVLQQWHAAAAQEQPACVLGNLPYNIAARFIGNTIESGYIFKRMVVTVQKEIGLRMTALP
AQKWYSYFSVLCQWQYEVRVIRNVAPVCFWPRPHVVSQALVLTKRNAVPSCVDPALFLHVTKTLFSARRKTVRNNLLTWQ
KRMPGGAAVCVEELCARAGIDARARAEQLSIYDFITLSDTLRALL
>Mature_285_residues
MCCPNYNSARALAQFLTERGLRMHKKWGQNFLLDPVLRTQLVKILAPERGERVWEIGAGIGAMTALLVQNSDFLTVFEID
RGFVQTLRKLFDAHVRVIEGDVLQQWHAAAAQEQPACVLGNLPYNIAARFIGNTIESGYIFKRMVVTVQKEIGLRMTALP
AQKWYSYFSVLCQWQYEVRVIRNVAPVCFWPRPHVVSQALVLTKRNAVPSCVDPALFLHVTKTLFSARRKTVRNNLLTWQ
KRMPGGAAVCVEELCARAGIDARARAEQLSIYDFITLSDTLRALL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=291, Percent_Identity=30.5841924398625, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI7657198, Length=284, Percent_Identity=25, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1786236, Length=269, Percent_Identity=30.4832713754647, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25141369, Length=297, Percent_Identity=24.9158249158249, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21358017, Length=199, Percent_Identity=27.1356783919598, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_TREPA (O83357)

Other databases:

- EMBL:   AE000520
- PIR:   G71337
- RefSeq:   NP_218777.1
- ProteinModelPortal:   O83357
- SMR:   O83357
- IntAct:   O83357
- GeneID:   2611818
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0337
- NMPDR:   fig|243276.1.peg.336
- TIGR:   TP_0337
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   TPAL243276:TP_0337-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 32276; Mature: 32276

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 30-30 BINDING 32-32 BINDING 57-57 BINDING 78-78 BINDING 101-101 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCCPNYNSARALAQFLTERGLRMHKKWGQNFLLDPVLRTQLVKILAPERGERVWEIGAGI
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCH
GAMTALLVQNSDFLTVFEIDRGFVQTLRKLFDAHVRVIEGDVLQQWHAAAAQEQPACVLG
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
NLPYNIAARFIGNTIESGYIFKRMVVTVQKEIGLRMTALPAQKWYSYFSVLCQWQYEVRV
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHH
IRNVAPVCFWPRPHVVSQALVLTKRNAVPSCVDPALFLHVTKTLFSARRKTVRNNLLTWQ
HHCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRMPGGAAVCVEELCARAGIDARARAEQLSIYDFITLSDTLRALL
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MCCPNYNSARALAQFLTERGLRMHKKWGQNFLLDPVLRTQLVKILAPERGERVWEIGAGI
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCH
GAMTALLVQNSDFLTVFEIDRGFVQTLRKLFDAHVRVIEGDVLQQWHAAAAQEQPACVLG
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
NLPYNIAARFIGNTIESGYIFKRMVVTVQKEIGLRMTALPAQKWYSYFSVLCQWQYEVRV
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHH
IRNVAPVCFWPRPHVVSQALVLTKRNAVPSCVDPALFLHVTKTLFSARRKTVRNNLLTWQ
HHCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRMPGGAAVCVEELCARAGIDARARAEQLSIYDFITLSDTLRALL
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876