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The map label for this gene is smpB

Identifier: 189025417

GI number: 189025417

Start: 201706

End: 202170

Strand: Reverse

Name: smpB

Synonym: TPASS_0184

Alternate gene names: 189025417

Gene position: 202170-201706 (Counterclockwise)

Preceding gene: 189025459

Following gene: 189025416

Centisome position: 17.74

GC content: 55.27

Gene sequence:

>465_bases
ATGAGGGGCACAGGAACCCATCTCATTGCAAAGAACCGCAAGGCTTTTTTCAATTACCATGTGGAAGACCGTCTAGAGTG
TGGCATCGCGCTTGAAGGCACGGAGGTGAAGTCTGTGCGCGCGGGGCATCTTTCTTTTCCTGACGCATTTGCAGAGATGC
GCGGAGGCGAGCTGTGGCTAAAGAATGTGCATATTGCGGAGTACGTGCATGCTTGCTCCTTTGCCCCCAATCCGGATCGG
ATGCGGAAGTTACTGGCACATCGGGACCAGATTGCGCGCTTGAAGCGCAAGGTTGAAGAGAAAGGCTATACTCTGGTCCC
CCTGGAGTTTTACCTAAAAGCAGGGCGGGTGAAGGTAGCGCTGGGAATCTGCAAGGGCAAGAAGCTTTTTGACAAGCGCG
CGCAGATCAAAGCTCGCGACAACGCACGGGAGCTGTCCCGCAGCCTCTGTGAGCGACACCACTAG

Upstream 100 bases:

>100_bases
ACCGCGCAAACATAAAACGGCGTCCCTCCCAGACACGGCAGCCTAACACGGCGATAATCGAATTGACAAGGGAAGTGCCT
ATCCGGTAGCATCCGGCGCC

Downstream 100 bases:

>100_bases
TCTCATGAAAAAAGGTGTCCGTTCTTCTCGGCTGCTTATCCTGTTCGTGCTGTTCGCGCATGCTGTTCACGCAGCTCCTC
GTGTTGGCGTCTACAGGCTA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MRGTGTHLIAKNRKAFFNYHVEDRLECGIALEGTEVKSVRAGHLSFPDAFAEMRGGELWLKNVHIAEYVHACSFAPNPDR
MRKLLAHRDQIARLKRKVEEKGYTLVPLEFYLKAGRVKVALGICKGKKLFDKRAQIKARDNARELSRSLCERHH

Sequences:

>Translated_154_residues
MRGTGTHLIAKNRKAFFNYHVEDRLECGIALEGTEVKSVRAGHLSFPDAFAEMRGGELWLKNVHIAEYVHACSFAPNPDR
MRKLLAHRDQIARLKRKVEEKGYTLVPLEFYLKAGRVKVALGICKGKKLFDKRAQIKARDNARELSRSLCERHH
>Mature_154_residues
MRGTGTHLIAKNRKAFFNYHVEDRLECGIALEGTEVKSVRAGHLSFPDAFAEMRGGELWLKNVHIAEYVHACSFAPNPDR
MRKLLAHRDQIARLKRKVEEKGYTLVPLEFYLKAGRVKVALGICKGKKLFDKRAQIKARDNARELSRSLCERHH

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=150, Percent_Identity=37.3333333333333, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_TREPA (O83214)

Other databases:

- EMBL:   AE000520
- PIR:   B71354
- RefSeq:   NP_218623.2
- ProteinModelPortal:   O83214
- SMR:   O83214
- IntAct:   O83214
- GeneID:   2611245
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0184
- NMPDR:   fig|243276.1.peg.182
- TIGR:   TP_0184
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   TPAL243276:TP_0184-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17654; Mature: 17654

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGTGTHLIAKNRKAFFNYHVEDRLECGIALEGTEVKSVRAGHLSFPDAFAEMRGGELWL
CCCCCCEEEECCCHHEEEECCHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHCCCCEEEE
KNVHIAEYVHACSFAPNPDRMRKLLAHRDQIARLKRKVEEKGYTLVPLEFYLKAGRVKVA
ECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCEEEE
LGICKGKKLFDKRAQIKARDNARELSRSLCERHH
EEECCCHHHHHHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRGTGTHLIAKNRKAFFNYHVEDRLECGIALEGTEVKSVRAGHLSFPDAFAEMRGGELWL
CCCCCCEEEECCCHHEEEECCHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHCCCCEEEE
KNVHIAEYVHACSFAPNPDRMRKLLAHRDQIARLKRKVEEKGYTLVPLEFYLKAGRVKVA
ECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCEEEE
LGICKGKKLFDKRAQIKARDNARELSRSLCERHH
EEECCCHHHHHHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876