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The map label for this gene is ruvB

Identifier: 189025395

GI number: 189025395

Start: 184567

End: 185619

Strand: Reverse

Name: ruvB

Synonym: TPASS_0162

Alternate gene names: 189025395

Gene position: 185619-184567 (Counterclockwise)

Preceding gene: 189025405

Following gene: 189025394

Centisome position: 16.29

GC content: 58.59

Gene sequence:

>1053_bases
ATGGATCACACCGCGTCACTGAGTCCTGTGCGCCCTGAAGCACAACCTACAGACGATCGCGAGCGTGCGCTCAGACCGCG
CCTCCTGAAAGACTTTCTAGGTCAGGAGAAAACAAAACGCAACTTACGTCTTTTCATTCAGGCAGCGCGCGATCGCAACG
AAAGCTTAGATCACCTGTTCCTCATCGGCCCCCCGGGGCTCGGCAAAACGACGCTCGCGCATATCACTGCATGCGAGCTG
GGCGTTGAGTGCAAGGTTACAGGCGCACCGGCGCTTGATAAACCAAAAGATTTAGCGGGTATCCTCACTGCGCTGAGTGA
GCGAAGCGTCTTCTTCGTGGATGAAATCCACCGCCTCAAACCAGCCATAGAAGAGATGCTGTACATTGCCATGGAGGACT
ACGAACTGGATTGGGTTATCGGTCAGGGACCGTCCGCGCGCACGGTGCGCATCCCACTCCCCCCGTTTACCCTCATTGGT
GCAACCACTCGCGCGGGTATGGTTTCAAGCCCGCTGATTAGCCGCTTTGGAATCGTAGAGCGCTTCGAGTTCTATACCCC
TGAGGAGCTTGCTGCCATTGTGCAACGCTCAGCGCGGCTTCTAGATATCACGCTCGACGCACGCGCAGCGTTAGCCCTTG
CGCGGTGTTCGCGAGGAACACCCCGGGTGGCCAACCGGCTTTTGCGCCGTATACGCGATTTTGCCCAAGTTGCGGGGTCT
GCACACATCAGCGAGACGATAGTACGCGCAGGGCTTGCCCACCTAAAGATCGACGAATTAGGGCTAGAACTGCACGACAT
ACAGCTGCTGCGCGTCATGATTGAGCACTTCGGCGGAGGGCCAGTGGGCGCAGAAACGCTGGCGATCTCCCTCGGGGAAT
CACCGGAAACACTTGAGGATTACTACGAGCCCTACCTTATCCAAATTGGGCTCATGCAGCGCACCCCCCGCGGGCGCATG
GCCACCGCGCGTGCCTATGCGCACCTAGGTCTCCCTGTCCCCGAGGCACGCACGCTCACCCCGCACTCCCCAGAACAAGG
AACGCTTCTTTAG

Upstream 100 bases:

>100_bases
TTCAGGAAAAGAAAACGGCACTCTGGCGCGGACCTCTCGAGCTACATGATAAAGAAGCATTTGATCTTCCTCGCATAAGC
AGCTACAGTTGCGCCCTCTT

Downstream 100 bases:

>100_bases
CAAAGATGCGGACACCTTGTCAGAGCTGTCGGCAGCTCCGCTCAGACCGGGTAAGACACAAGAGTCTGAAAAAGGCATAT
ACTACGCGCGGGAGGGGTGC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MDHTASLSPVRPEAQPTDDRERALRPRLLKDFLGQEKTKRNLRLFIQAARDRNESLDHLFLIGPPGLGKTTLAHITACEL
GVECKVTGAPALDKPKDLAGILTALSERSVFFVDEIHRLKPAIEEMLYIAMEDYELDWVIGQGPSARTVRIPLPPFTLIG
ATTRAGMVSSPLISRFGIVERFEFYTPEELAAIVQRSARLLDITLDARAALALARCSRGTPRVANRLLRRIRDFAQVAGS
AHISETIVRAGLAHLKIDELGLELHDIQLLRVMIEHFGGGPVGAETLAISLGESPETLEDYYEPYLIQIGLMQRTPRGRM
ATARAYAHLGLPVPEARTLTPHSPEQGTLL

Sequences:

>Translated_350_residues
MDHTASLSPVRPEAQPTDDRERALRPRLLKDFLGQEKTKRNLRLFIQAARDRNESLDHLFLIGPPGLGKTTLAHITACEL
GVECKVTGAPALDKPKDLAGILTALSERSVFFVDEIHRLKPAIEEMLYIAMEDYELDWVIGQGPSARTVRIPLPPFTLIG
ATTRAGMVSSPLISRFGIVERFEFYTPEELAAIVQRSARLLDITLDARAALALARCSRGTPRVANRLLRRIRDFAQVAGS
AHISETIVRAGLAHLKIDELGLELHDIQLLRVMIEHFGGGPVGAETLAISLGESPETLEDYYEPYLIQIGLMQRTPRGRM
ATARAYAHLGLPVPEARTLTPHSPEQGTLL
>Mature_350_residues
MDHTASLSPVRPEAQPTDDRERALRPRLLKDFLGQEKTKRNLRLFIQAARDRNESLDHLFLIGPPGLGKTTLAHITACEL
GVECKVTGAPALDKPKDLAGILTALSERSVFFVDEIHRLKPAIEEMLYIAMEDYELDWVIGQGPSARTVRIPLPPFTLIG
ATTRAGMVSSPLISRFGIVERFEFYTPEELAAIVQRSARLLDITLDARAALALARCSRGTPRVANRLLRRIRDFAQVAGS
AHISETIVRAGLAHLKIDELGLELHDIQLLRVMIEHFGGGPVGAETLAISLGESPETLEDYYEPYLIQIGLMQRTPRGRM
ATARAYAHLGLPVPEARTLTPHSPEQGTLL

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=320, Percent_Identity=54.375, Blast_Score=356, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_TREPA (P96115)

Other databases:

- EMBL:   AE000520
- EMBL:   U55214
- PIR:   H71359
- RefSeq:   NP_218601.1
- ProteinModelPortal:   P96115
- SMR:   P96115
- IntAct:   P96115
- MINT:   MINT-6479174
- GeneID:   2611116
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0162
- NMPDR:   fig|243276.1.peg.160
- TIGR:   TP_0162
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   TPAL243276:TP_0162-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF00004 AAA; PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 38665; Mature: 38665

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00690 DEAH_ATP_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHTASLSPVRPEAQPTDDRERALRPRLLKDFLGQEKTKRNLRLFIQAARDRNESLDHLF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEE
LIGPPGLGKTTLAHITACELGVECKVTGAPALDKPKDLAGILTALSERSVFFVDEIHRLK
EECCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEHHHHHHHHH
PAIEEMLYIAMEDYELDWVIGQGPSARTVRIPLPPFTLIGATTRAGMVSSPLISRFGIVE
HHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCCEEEECCCHHCCCHHHHHHHHHHHHH
RFEFYTPEELAAIVQRSARLLDITLDARAALALARCSRGTPRVANRLLRRIRDFAQVAGS
HHHCCCHHHHHHHHHCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
AHISETIVRAGLAHLKIDELGLELHDIQLLRVMIEHFGGGPVGAETLAISLGESPETLED
HHHHHHHHHHHHHHEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHH
YYEPYLIQIGLMQRTPRGRMATARAYAHLGLPVPEARTLTPHSPEQGTLL
HCCCEEEEEEHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDHTASLSPVRPEAQPTDDRERALRPRLLKDFLGQEKTKRNLRLFIQAARDRNESLDHLF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEE
LIGPPGLGKTTLAHITACELGVECKVTGAPALDKPKDLAGILTALSERSVFFVDEIHRLK
EECCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEHHHHHHHHH
PAIEEMLYIAMEDYELDWVIGQGPSARTVRIPLPPFTLIGATTRAGMVSSPLISRFGIVE
HHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCCEEEECCCHHCCCHHHHHHHHHHHHH
RFEFYTPEELAAIVQRSARLLDITLDARAALALARCSRGTPRVANRLLRRIRDFAQVAGS
HHHCCCHHHHHHHHHCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
AHISETIVRAGLAHLKIDELGLELHDIQLLRVMIEHFGGGPVGAETLAISLGESPETLED
HHHHHHHHHHHHHHEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHH
YYEPYLIQIGLMQRTPRGRMATARAYAHLGLPVPEARTLTPHSPEQGTLL
HCCCEEEEEEHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876; 9332349