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The map label for this gene is proS

Identifier: 189025393

GI number: 189025393

Start: 182602

End: 184455

Strand: Reverse

Name: proS

Synonym: TPASS_0160

Alternate gene names: 189025393

Gene position: 184455-182602 (Counterclockwise)

Preceding gene: 189025394

Following gene: 189025392

Centisome position: 16.19

GC content: 55.99

Gene sequence:

>1854_bases
ATGTCTGCGTTTTTTGCACCAACCCTGCGGTCTGCACCTGCTGATGCAACCATCGCAAGCCACCAGCTGCTCATGCGCGC
AGGGTACGTCAGAAAAATCGCCAACGGCCTGTTTGCGTACCTTCCCCTGGGCCTGCGCGTTCGACACAAAATTGAAGCGA
TTATTCGGGAAGAACTCGAGGCTATCGGGTGTTTGGAGTGCACCGCGCCTGTCGTGACTCCTGCAGAGTTGTGGAAGGAA
TCTGGCCGCTGGTACCGCATGGGCGCAGAGCTTTTGCGCGCCAAAAATCGGCTCGATCACGAGCTCCTTTTCAGTCCGAC
TGCAGAAGAATCCTTCACCGCTTTGGTGCGCGGCGACTGTACTTCCTACAAACATTTTCCCCTCAGTCTCTACCAAATCA
ACGCAAAATATCGCGATGAAATCCGTCCGCGTTACGGACTGATGCGCGCGCGCGAGTTCACCATGGCCGACGCCTATTCT
TTCCACACAGACTGCGCATGCCTTGCGCGCACGTACGAAAAGTTTGCGCACGCGTATCGCGCCATTTTCCGTCGCATCGG
CCTATCAGTCATTGCAGTACATGCACACCTCGGTGCGATGGGGGGGCAGGAATCCGAGGAATTCATGGTAGAGTCCGCGG
TGGGCGACAACACGCTCCTGTTGTGTCCCCACTGCACCTACGCTGCAAATTGCGAAAAGGCCGTCGGACAGCGCCCCCTC
CCAGACACGCATGACACTCATCTAAAAGACGAACACGAAGGGTCAGATCTCAAGACGCCTGCAGCAATGCGCGAGGTGCA
CACCCCGCACGTGAAAACTATTGAGGAACTTGAACACTTCTTGCACGTACCTGCACATCGCTGCATCAAGACGCTTATTT
ACCGCATTGACACGGTGCCCCAGGCGGCTGGGCATTTTGTGGCAGTGTGCATCCGCGGCGACCTAGAACTCAACGAGTCA
AAGCTCGAAGCGCTCCTGCGCGTGCCATCTGTAGTACTGGCAACTGAACAAGAGGTGTATGCACTCAGCGGCACCCCCGT
AGGATTCATTGGTCCGGTAGGACTTGCACAGCGTGCTGCAGCTGCGTATGCCGCTCGCACCCCTGCGTTCTTCCCCTCCG
CTGCTGAGCCTGCATCCGTCACTTCTGACATTCCATTTTTTTCCCTCGTTGCAGATCAGTCCGTGATGGCTATGCACAAC
GCTATCACCGGTGCGTTGAAAGTTGACACGCATCTTGTGCAGGTAGAACCGGGTCGAGACTTTGTTCCTGACGCAGTTGC
AGATCTCATGCTCGTGCGCGCCGGCGACCGGTGCATACACTGTGGAGCGCCCCTATACGAAAAAAAGGGTAACGAACTAG
GTCACCTCTTTAAATTAGGGGACAAATACACGCGCAGCATGCACCTTACCTTTACTGATGAGCAGGGTGTACGACAGTTC
CCCCTGATGGGCTGCTATGGCATTGGCCTTGATCGCACGCTTGCCTCTGTGGTGGAAAACCACCATGACACGCGGGGTAT
CAGCTGGCCGCTTGCGATCAGCCCCTATGCAGTTGTGCTCATACCCATCCCTCACACGCAGGCCCCCTATGCAGCAGCAG
AGGCACTGTACGTGCAGCTGCGGACACGGGGAGTTGAGGTACTGTTTGATGATCGTGCAGAGCGACCCGGAGTAAAGTTC
GCAGACGCTGATTTAATCGGTATTCCCCTTCGTGTGGTACTGAGTGCGAAAACGCTACCGCGCGTTGAATGCACAACACG
GTGTGGTGCGCACACGTATTTTTTTACGCAAGAAGAGGCGTCCGAGCACATTGCACGCCTGCTCGAACAACTCGCTTCCC
CGGAAAGTTCGTAA

Upstream 100 bases:

>100_bases
ACACCTTGTCAGAGCTGTCGGCAGCTCCGCTCAGACCGGGTAAGACACAAGAGTCTGAAAAAGGCATATACTACGCGCGG
GAGGGGTGCGTTTCGTGAAG

Downstream 100 bases:

>100_bases
GAACGGGAATGCCGGAGCGGGATCCAGCGCATGCAGTGCTGAGACCTGCGCATAATAGCACAGTGTACGGCACCCGTGGT
TTAGAAAAAAATGACGAAGG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MSAFFAPTLRSAPADATIASHQLLMRAGYVRKIANGLFAYLPLGLRVRHKIEAIIREELEAIGCLECTAPVVTPAELWKE
SGRWYRMGAELLRAKNRLDHELLFSPTAEESFTALVRGDCTSYKHFPLSLYQINAKYRDEIRPRYGLMRAREFTMADAYS
FHTDCACLARTYEKFAHAYRAIFRRIGLSVIAVHAHLGAMGGQESEEFMVESAVGDNTLLLCPHCTYAANCEKAVGQRPL
PDTHDTHLKDEHEGSDLKTPAAMREVHTPHVKTIEELEHFLHVPAHRCIKTLIYRIDTVPQAAGHFVAVCIRGDLELNES
KLEALLRVPSVVLATEQEVYALSGTPVGFIGPVGLAQRAAAAYAARTPAFFPSAAEPASVTSDIPFFSLVADQSVMAMHN
AITGALKVDTHLVQVEPGRDFVPDAVADLMLVRAGDRCIHCGAPLYEKKGNELGHLFKLGDKYTRSMHLTFTDEQGVRQF
PLMGCYGIGLDRTLASVVENHHDTRGISWPLAISPYAVVLIPIPHTQAPYAAAEALYVQLRTRGVEVLFDDRAERPGVKF
ADADLIGIPLRVVLSAKTLPRVECTTRCGAHTYFFTQEEASEHIARLLEQLASPESS

Sequences:

>Translated_617_residues
MSAFFAPTLRSAPADATIASHQLLMRAGYVRKIANGLFAYLPLGLRVRHKIEAIIREELEAIGCLECTAPVVTPAELWKE
SGRWYRMGAELLRAKNRLDHELLFSPTAEESFTALVRGDCTSYKHFPLSLYQINAKYRDEIRPRYGLMRAREFTMADAYS
FHTDCACLARTYEKFAHAYRAIFRRIGLSVIAVHAHLGAMGGQESEEFMVESAVGDNTLLLCPHCTYAANCEKAVGQRPL
PDTHDTHLKDEHEGSDLKTPAAMREVHTPHVKTIEELEHFLHVPAHRCIKTLIYRIDTVPQAAGHFVAVCIRGDLELNES
KLEALLRVPSVVLATEQEVYALSGTPVGFIGPVGLAQRAAAAYAARTPAFFPSAAEPASVTSDIPFFSLVADQSVMAMHN
AITGALKVDTHLVQVEPGRDFVPDAVADLMLVRAGDRCIHCGAPLYEKKGNELGHLFKLGDKYTRSMHLTFTDEQGVRQF
PLMGCYGIGLDRTLASVVENHHDTRGISWPLAISPYAVVLIPIPHTQAPYAAAEALYVQLRTRGVEVLFDDRAERPGVKF
ADADLIGIPLRVVLSAKTLPRVECTTRCGAHTYFFTQEEASEHIARLLEQLASPESS
>Mature_616_residues
SAFFAPTLRSAPADATIASHQLLMRAGYVRKIANGLFAYLPLGLRVRHKIEAIIREELEAIGCLECTAPVVTPAELWKES
GRWYRMGAELLRAKNRLDHELLFSPTAEESFTALVRGDCTSYKHFPLSLYQINAKYRDEIRPRYGLMRAREFTMADAYSF
HTDCACLARTYEKFAHAYRAIFRRIGLSVIAVHAHLGAMGGQESEEFMVESAVGDNTLLLCPHCTYAANCEKAVGQRPLP
DTHDTHLKDEHEGSDLKTPAAMREVHTPHVKTIEELEHFLHVPAHRCIKTLIYRIDTVPQAAGHFVAVCIRGDLELNESK
LEALLRVPSVVLATEQEVYALSGTPVGFIGPVGLAQRAAAAYAARTPAFFPSAAEPASVTSDIPFFSLVADQSVMAMHNA
ITGALKVDTHLVQVEPGRDFVPDAVADLMLVRAGDRCIHCGAPLYEKKGNELGHLFKLGDKYTRSMHLTFTDEQGVRQFP
LMGCYGIGLDRTLASVVENHHDTRGISWPLAISPYAVVLIPIPHTQAPYAAAEALYVQLRTRGVEVLFDDRAERPGVKFA
DADLIGIPLRVVLSAKTLPRVECTTRCGAHTYFFTQEEASEHIARLLEQLASPESS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=38.0530973451327, Blast_Score=180, Evalue=3e-45,
Organism=Escherichia coli, GI1786392, Length=586, Percent_Identity=40.4436860068259, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI115532348, Length=226, Percent_Identity=36.7256637168142, Blast_Score=152, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320931, Length=223, Percent_Identity=34.5291479820628, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24656200, Length=257, Percent_Identity=33.4630350194552, Blast_Score=155, Evalue=5e-38,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_TREPA (O83195)

Other databases:

- EMBL:   AE000520
- PIR:   F71359
- RefSeq:   NP_218599.1
- ProteinModelPortal:   O83195
- SMR:   O83195
- IntAct:   O83195
- MINT:   MINT-6479150
- GeneID:   2611109
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0160
- NMPDR:   fig|243276.1.peg.158
- TIGR:   TP_0160
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   TPAL243276:TP_0160-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 68194; Mature: 68063

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFFAPTLRSAPADATIASHQLLMRAGYVRKIANGLFAYLPLGLRVRHKIEAIIREELE
CCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AIGCLECTAPVVTPAELWKESGRWYRMGAELLRAKNRLDHELLFSPTAEESFTALVRGDC
HHCHHHCCCCCCCHHHHHHHCCCEEHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHCCCC
TSYKHFPLSLYQINAKYRDEIRPRYGLMRAREFTMADAYSFHTDCACLARTYEKFAHAYR
CCCCCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AIFRRIGLSVIAVHAHLGAMGGQESEEFMVESAVGDNTLLLCPHCTYAANCEKAVGQRPL
HHHHHHCHHHEEHHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCHHHHHCCCCC
PDTHDTHLKDEHEGSDLKTPAAMREVHTPHVKTIEELEHFLHVPAHRCIKTLIYRIDTVP
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
QAAGHFVAVCIRGDLELNESKLEALLRVPSVVLATEQEVYALSGTPVGFIGPVGLAQRAA
CCCCCEEEEEEECCCCCCHHHHHHHHHCCHHEEECCCCEEEECCCCCCEECCHHHHHHHH
AAYAARTPAFFPSAAEPASVTSDIPFFSLVADQSVMAMHNAITGALKVDTHLVQVEPGRD
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHCEEEECEEEEEECCCCC
FVPDAVADLMLVRAGDRCIHCGAPLYEKKGNELGHLFKLGDKYTRSMHLTFTDEQGVRQF
CCHHHHHHHHHHHCCCCEEECCCCHHHHCCCCHHHHHHHCCHHCEEEEEEECCCCCCCCC
PLMGCYGIGLDRTLASVVENHHDTRGISWPLAISPYAVVLIPIPHTQAPYAAAEALYVQL
CCCEEECCCHHHHHHHHHHCCCCCCCCCCCEEECCEEEEEEECCCCCCCHHHHHHHHHHH
RTRGVEVLFDDRAERPGVKFADADLIGIPLRVVLSAKTLPRVECTTRCGAHTYFFTQEEA
HHCCEEEEECCCCCCCCCEEECCCEECCCHHEEEECCCCCCCCHHHCCCCCEEEEEHHHH
SEHIARLLEQLASPESS
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SAFFAPTLRSAPADATIASHQLLMRAGYVRKIANGLFAYLPLGLRVRHKIEAIIREELE
CCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AIGCLECTAPVVTPAELWKESGRWYRMGAELLRAKNRLDHELLFSPTAEESFTALVRGDC
HHCHHHCCCCCCCHHHHHHHCCCEEHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHCCCC
TSYKHFPLSLYQINAKYRDEIRPRYGLMRAREFTMADAYSFHTDCACLARTYEKFAHAYR
CCCCCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AIFRRIGLSVIAVHAHLGAMGGQESEEFMVESAVGDNTLLLCPHCTYAANCEKAVGQRPL
HHHHHHCHHHEEHHHHHHCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCHHHHHCCCCC
PDTHDTHLKDEHEGSDLKTPAAMREVHTPHVKTIEELEHFLHVPAHRCIKTLIYRIDTVP
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
QAAGHFVAVCIRGDLELNESKLEALLRVPSVVLATEQEVYALSGTPVGFIGPVGLAQRAA
CCCCCEEEEEEECCCCCCHHHHHHHHHCCHHEEECCCCEEEECCCCCCEECCHHHHHHHH
AAYAARTPAFFPSAAEPASVTSDIPFFSLVADQSVMAMHNAITGALKVDTHLVQVEPGRD
HHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHCEEEECEEEEEECCCCC
FVPDAVADLMLVRAGDRCIHCGAPLYEKKGNELGHLFKLGDKYTRSMHLTFTDEQGVRQF
CCHHHHHHHHHHHCCCCEEECCCCHHHHCCCCHHHHHHHCCHHCEEEEEEECCCCCCCCC
PLMGCYGIGLDRTLASVVENHHDTRGISWPLAISPYAVVLIPIPHTQAPYAAAEALYVQL
CCCEEECCCHHHHHHHHHHCCCCCCCCCCCEEECCEEEEEEECCCCCCCHHHHHHHHHHH
RTRGVEVLFDDRAERPGVKFADADLIGIPLRVVLSAKTLPRVECTTRCGAHTYFFTQEEA
HHCCEEEEECCCCCCCCCEEECCCEECCCHHEEEECCCCCCCCHHHCCCCCEEEEEHHHH
SEHIARLLEQLASPESS
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876