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The map label for this gene is fhlA [H]

Identifier: 189025316

GI number: 189025316

Start: 90626

End: 92428

Strand: Reverse

Name: fhlA [H]

Synonym: TPASS_0082

Alternate gene names: 189025316

Gene position: 92428-90626 (Counterclockwise)

Preceding gene: 189025318

Following gene: 189025307

Centisome position: 8.11

GC content: 52.72

Gene sequence:

>1803_bases
TTGCACCGCCTGCGAGAAATTCTCCCTGCGCATTTGCCCATACGCAAGGAAACATCAGAACACTTATCCACACGCGTCTC
ATGCGCGCGATTATACCCCATAWAAAACGCGAGGAAAAGGATTCGAAAAACAAATACCATACCCTGCACACGACACGCCC
ACAGGCTCCATGCGGAGAATTGCACGGATATGCCTTTTTCTTCTATACTCGGGCCCCATGAACTGTTCGGGCTGTCCTGC
ACGCGCGCTCTCCCCCCTGCTGCGGAAAAGTCGCTGGAAATGATTCTGGACGCCCTCTATGAGATTGCCCGTTACGAGCT
AGCCGTGGTCCTCAGCTTTGAATCTTCTCAAATACTGCGAGTTCGCAAGGCGCGTGGTCCCCTGTACACTCCCCGCCTCC
AACACCACACCATAGACCTCACCAAAAGGCAGGATCTTGCGCGCATCCTATCACAGAAGAGTCCATACCTTTTCGATCCG
ACGCTTGCCCACACTGATACCTACACAGAGCTTATTCCCATGCCCCAAAATCATTCCTGTCTCATCGCGCCGCTTTACAT
CGATGATACTCCCATTGGCATGCTCACGCTCGACCATCGCCTGTGCGAGCAGTTTACCCCAGACGTTGTACGCTTCATTA
CTACTCTTTCTAAACTTATCTCGCTCGCAGTAGCCCAGACCGACGCGTCCGAGACACTGAGTCAAAAGAGTCACGCTCTT
TTGACCGAACGAAATGCGCTCTTAGCTCCCCAATCACCTGCATTTAAAAATATGATCGGAACCTCTCCTGCTTGGACACG
CACACTCGATGCTATAAAGCTCGTAGCAGCCTCTGACCTCCCTGTCCTTGTTTGTGGAGAAACCGGCACTGGAAAGGAGC
TTGTTGCGCGAACCGTTCACCAACTTTCCACCCGCAGCGACAAGCCCTTCGTCCCCGTCAATTGCTCAGCACTCGTCCAT
AGCCTTGCAGAAAGCGAATTATTCGGACATGAAAAAGGTGCCTTCTCAGGTGCGGTCGGCACCCATCGGGGAAGATTTGA
ACTGGCTCACGGTGGCACCCTTTTTCTCGATGAGGTCGGAGATCTCCCCCTGGAACTGCAACCGAAGCTCCTCCGCGTAC
TCCAAGACGGAGTATTTGAGCGCGTTGGAGGGGAGCGTTCGGTAAGCGTCTCGGTTCGCATTATTGCAGCTACGAATATC
AACCTTTCAGAAGCAGTCACCCAAGGGAAATTCAGGGAAGACCTCCTCTACCGGCTCGATGTTTTTCCCCTCAGTCTCCC
CCCTTTGCGCGAGCGTGCAGAAGACGTTGCGCTTCTAGCGGAGCATTTTATCCAAAAAATTAAGACCCGCCCGGGGTTTG
AGCGCACTCAGCTTTCTCCCAGCGCGTTTAAGAAAATCTTCTCGCTCACGTTTCCTGGCAATGTGCGTGAGTTACGCAAC
CTACTCGAGCGTGCTGCACTTCTTGCACGCGGAGATACCATCGGCGCAGAACACCTTGTATGTAGGACCCCGGAATCCGG
CACGCAGACTACTGTCTATCCTCGGGAGCACACCGAAGTGTCAGAAAGCGTTCAACCAGCTATACCTCTGTGCATACGCG
ATAGCTCTTGCATGGTGCGATGCACACAGGGGAAAAAGGAAGAAACTACGCTGGTGGTCAACTTTCATCAGGCGCAACGC
GCAGCAATTCAAGCGGCGCTTGATGCAAGCAACGGTAAGATATACGGAGCAAATGGCGCAGCAGAAATTCTTGGTCTTAA
GCCAAGTACATTGCAAAGCAAAATGAAAAAACTCGGTCTTTAG

Upstream 100 bases:

>100_bases
GTAAGCAAGAACCAGTTCTCTCCCGCAGCAATAATCTTGCTAACCGAAACGCCAGAGAGCAGTACGTCTTGAGCGTAAGG
AGTAATACGGTACAATCCCT

Downstream 100 bases:

>100_bases
CGTCAGCTGTTTGCGCGTGCCTGGGTAAAACTCGCCACCTGTAGCTTCATCTTAAGCGGCGCAAACAGTCTTCCAGCAGA
TGAAAGAAGCAGTGCGCGAT

Product: formate hydrogenlyase transcriptional activator FhlA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MHRLREILPAHLPIRKETSEHLSTRVSCARLYPIXNARKRIRKTNTIPCTRHAHRLHAENCTDMPFSSILGPHELFGLSC
TRALPPAAEKSLEMILDALYEIARYELAVVLSFESSQILRVRKARGPLYTPRLQHHTIDLTKRQDLARILSQKSPYLFDP
TLAHTDTYTELIPMPQNHSCLIAPLYIDDTPIGMLTLDHRLCEQFTPDVVRFITTLSKLISLAVAQTDASETLSQKSHAL
LTERNALLAPQSPAFKNMIGTSPAWTRTLDAIKLVAASDLPVLVCGETGTGKELVARTVHQLSTRSDKPFVPVNCSALVH
SLAESELFGHEKGAFSGAVGTHRGRFELAHGGTLFLDEVGDLPLELQPKLLRVLQDGVFERVGGERSVSVSVRIIAATNI
NLSEAVTQGKFREDLLYRLDVFPLSLPPLRERAEDVALLAEHFIQKIKTRPGFERTQLSPSAFKKIFSLTFPGNVRELRN
LLERAALLARGDTIGAEHLVCRTPESGTQTTVYPREHTEVSESVQPAIPLCIRDSSCMVRCTQGKKEETTLVVNFHQAQR
AAIQAALDASNGKIYGANGAAEILGLKPSTLQSKMKKLGL

Sequences:

>Translated_600_residues
MHRLREILPAHLPIRKETSEHLSTRVSCARLYPIXNARKRIRKTNTIPCTRHAHRLHAENCTDMPFSSILGPHELFGLSC
TRALPPAAEKSLEMILDALYEIARYELAVVLSFESSQILRVRKARGPLYTPRLQHHTIDLTKRQDLARILSQKSPYLFDP
TLAHTDTYTELIPMPQNHSCLIAPLYIDDTPIGMLTLDHRLCEQFTPDVVRFITTLSKLISLAVAQTDASETLSQKSHAL
LTERNALLAPQSPAFKNMIGTSPAWTRTLDAIKLVAASDLPVLVCGETGTGKELVARTVHQLSTRSDKPFVPVNCSALVH
SLAESELFGHEKGAFSGAVGTHRGRFELAHGGTLFLDEVGDLPLELQPKLLRVLQDGVFERVGGERSVSVSVRIIAATNI
NLSEAVTQGKFREDLLYRLDVFPLSLPPLRERAEDVALLAEHFIQKIKTRPGFERTQLSPSAFKKIFSLTFPGNVRELRN
LLERAALLARGDTIGAEHLVCRTPESGTQTTVYPREHTEVSESVQPAIPLCIRDSSCMVRCTQGKKEETTLVVNFHQAQR
AAIQAALDASNGKIYGANGAAEILGLKPSTLQSKMKKLGL
>Mature_600_residues
MHRLREILPAHLPIRKETSEHLSTRVSCARLYPIXNARKRIRKTNTIPCTRHAHRLHAENCTDMPFSSILGPHELFGLSC
TRALPPAAEKSLEMILDALYEIARYELAVVLSFESSQILRVRKARGPLYTPRLQHHTIDLTKRQDLARILSQKSPYLFDP
TLAHTDTYTELIPMPQNHSCLIAPLYIDDTPIGMLTLDHRLCEQFTPDVVRFITTLSKLISLAVAQTDASETLSQKSHAL
LTERNALLAPQSPAFKNMIGTSPAWTRTLDAIKLVAASDLPVLVCGETGTGKELVARTVHQLSTRSDKPFVPVNCSALVH
SLAESELFGHEKGAFSGAVGTHRGRFELAHGGTLFLDEVGDLPLELQPKLLRVLQDGVFERVGGERSVSVSVRIIAATNI
NLSEAVTQGKFREDLLYRLDVFPLSLPPLRERAEDVALLAEHFIQKIKTRPGFERTQLSPSAFKKIFSLTFPGNVRELRN
LLERAALLARGDTIGAEHLVCRTPESGTQTTVYPREHTEVSESVQPAIPLCIRDSSCMVRCTQGKKEETTLVVNFHQAQR
AAIQAALDASNGKIYGANGAAEILGLKPSTLQSKMKKLGL

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=476, Percent_Identity=41.3865546218487, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1789087, Length=473, Percent_Identity=39.323467230444, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI87082152, Length=339, Percent_Identity=46.9026548672566, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1788550, Length=264, Percent_Identity=47.7272727272727, Blast_Score=248, Evalue=1e-66,
Organism=Escherichia coli, GI1790299, Length=349, Percent_Identity=41.2607449856734, Blast_Score=238, Evalue=1e-63,
Organism=Escherichia coli, GI1790437, Length=271, Percent_Identity=46.8634686346863, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1788905, Length=231, Percent_Identity=47.1861471861472, Blast_Score=223, Evalue=4e-59,
Organism=Escherichia coli, GI87081872, Length=352, Percent_Identity=38.0681818181818, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1789233, Length=354, Percent_Identity=37.5706214689266, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1786524, Length=251, Percent_Identity=46.2151394422311, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1787583, Length=232, Percent_Identity=41.3793103448276, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87081858, Length=362, Percent_Identity=29.0055248618785, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1789828, Length=249, Percent_Identity=35.7429718875502, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 66364; Mature: 66364

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRLREILPAHLPIRKETSEHLSTRVSCARLYPIXNARKRIRKTNTIPCTRHAHRLHAEN
CCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHCCC
CTDMPFSSILGPHELFGLSCTRALPPAAEKSLEMILDALYEIARYELAVVLSFESSQILR
CCCCCHHHHCCHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHH
VRKARGPLYTPRLQHHTIDLTKRQDLARILSQKSPYLFDPTLAHTDTYTELIPMPQNHSC
HHHHCCCCCCCCCCCCEECHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHEECCCCCCCE
LIAPLYIDDTPIGMLTLDHRLCEQFTPDVVRFITTLSKLISLAVAQTDASETLSQKSHAL
EEEEEEECCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LTERNALLAPQSPAFKNMIGTSPAWTRTLDAIKLVAASDLPVLVCGETGTGKELVARTVH
HHCCCCEECCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHH
QLSTRSDKPFVPVNCSALVHSLAESELFGHEKGAFSGAVGTHRGRFELAHGGTLFLDEVG
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEEECCC
DLPLELQPKLLRVLQDGVFERVGGERSVSVSVRIIAATNINLSEAVTQGKFREDLLYRLD
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCHHHHHHHCHHHHHHHHHHC
VFPLSLPPLRERAEDVALLAEHFIQKIKTRPGFERTQLSPSAFKKIFSLTFPGNVRELRN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHEECCCCCHHHHHH
LLERAALLARGDTIGAEHLVCRTPESGTQTTVYPREHTEVSESVQPAIPLCIRDSSCMVR
HHHHHHHHHCCCCCCCCCEEEECCCCCCEEEECCCHHHHHHHHCCCCCCEEEECCCCEEE
CTQGKKEETTLVVNFHQAQRAAIQAALDASNGKIYGANGAAEILGLKPSTLQSKMKKLGL
ECCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHRLREILPAHLPIRKETSEHLSTRVSCARLYPIXNARKRIRKTNTIPCTRHAHRLHAEN
CCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHCCC
CTDMPFSSILGPHELFGLSCTRALPPAAEKSLEMILDALYEIARYELAVVLSFESSQILR
CCCCCHHHHCCHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHH
VRKARGPLYTPRLQHHTIDLTKRQDLARILSQKSPYLFDPTLAHTDTYTELIPMPQNHSC
HHHHCCCCCCCCCCCCEECHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHEECCCCCCCE
LIAPLYIDDTPIGMLTLDHRLCEQFTPDVVRFITTLSKLISLAVAQTDASETLSQKSHAL
EEEEEEECCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LTERNALLAPQSPAFKNMIGTSPAWTRTLDAIKLVAASDLPVLVCGETGTGKELVARTVH
HHCCCCEECCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHH
QLSTRSDKPFVPVNCSALVHSLAESELFGHEKGAFSGAVGTHRGRFELAHGGTLFLDEVG
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEEECCC
DLPLELQPKLLRVLQDGVFERVGGERSVSVSVRIIAATNINLSEAVTQGKFREDLLYRLD
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCHHHHHHHCHHHHHHHHHHC
VFPLSLPPLRERAEDVALLAEHFIQKIKTRPGFERTQLSPSAFKKIFSLTFPGNVRELRN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHEECCCCCHHHHHH
LLERAALLARGDTIGAEHLVCRTPESGTQTTVYPREHTEVSESVQPAIPLCIRDSSCMVR
HHHHHHHHHCCCCCCCCCEEEECCCCCCEEEECCCHHHHHHHHCCCCCCEEEECCCCEEE
CTQGKKEETTLVVNFHQAQRAAIQAALDASNGKIYGANGAAEILGLKPSTLQSKMKKLGL
ECCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]