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The map label for this gene is clpB

Identifier: 189025305

GI number: 189025305

Start: 75903

End: 78539

Strand: Reverse

Name: clpB

Synonym: TPASS_0071

Alternate gene names: 189025305

Gene position: 78539-75903 (Counterclockwise)

Preceding gene: 189025307

Following gene: 189025304

Centisome position: 6.89

GC content: 49.87

Gene sequence:

>2637_bases
ATGAACACAGACAGGTACACAGTCAAAGCAAGCGAAGCGCTCAATGACGCCATATCTCTGGCAGAAGCGGAGAACCACGG
TCAAGTTGAGGAGGAACATCTACTCCACGCCCTACTTTCCCAGAAAGACGGGATTATCTCTCCGCTCATTGAAAAAATTG
GGGCAAAACCGGACTTCCTGTACGATGAATTACTCCAATGCCTGCGCCGCAAACCACGTGTTACCGGTCCTGCCGCCCAA
ACGCGCTGTGCACCAACGCTGAGCAAAGCCTGTGCACGTGCAGAACGCCTCGCGCTCAAGAACCAAGATGAATATGTCTC
CTGCGAACATCTCCTGCTTGCCATAAGTGAGACAGATAGCAACACTGCACGTCTCCTTCACAGTCAGGGCATTACCAGTA
AAACTATCAGTGCCGCCCTCAAAGATATACGCGGCAGCAAGCGCGTTACGAGCCAGGATCCAGAATCAACATTCCAGTGC
TTGGAGAAATACTGCCGAGATCTTACTACCCTCGCCCGAGAAGAAAAAATAGATCCGGTTATTGGACGTGATGAAGAGAT
CCGGCGCGTTATGCAGGTACTCTCACGTCGTACAAAAAATAACCCAGTGCTTATTGGAGAACCCGGCGTAGGGAAAACCG
CTATTGTCGAGGGACTTGCACGCCGTATCGTTTCAGGAGACGTACCAGAAAGCCTCAAGGGAAAGCGTTTGCTTTCCCTT
GACCTCGGCGCATTGGTTGCCGGTGCAAAGTTCCGCGGGGAATTTGAAGAACGACTAAAAGCGGTAATTGAAGCGGTACA
GAAAAGCGACGGTGGCGTTATTTTATTCATTGATGAACTACACACGCTCGTAGGCGCCGGCGCAAGTGAGGGATCTATGG
ATGCGTCGAACCTTCTGAAACCTGCGCTTGCGCGCGGTGAATTGCGTTCAATCGGCGCAACCACGCTCAACGAATATCGC
AAATATATCGAAAAGGACGCAGCGCTCGAACGCCGCTTTCAGCAAGTGTACTGCGTACAGCCTACGGTGGAGGACACCAT
TGCTATCCTGCGCGGTTTGCAAGAAAAGTACGAAGTGCATCACGGGGTACGTATCAAAGATGAAGCGCTTGTTGCAGCAA
CCGTTTTGTCTGACCGTTACATCACCAACCGCTTTTTACCAGATAAGGCGATTGATCTGGTGGATGAAGCAGCAAGCCGC
CTGAAAATGGAAATTGAAAGTCAGCCTGTTGAGCTAGACCAGGTGGAGCGCAAGATATTACAGCTGAATATCGAAAAGGC
CTCTCTCCTTAAAGAAAGTGATCCGGCTTCAAAGGAACGTTTGGAAAAGTTAGAAAAAGAGCTCGCAGGCTTCCTAGAGC
GCCGTGCTGCAATGCAGGTCCAATGGCAAAATGAGAAAGGGAGGATAGAAGAGTCACGCCGCTACAAAGAGGAGCTTGAG
CGTCTCCGCATTGAGGAAACCATGTTTTCACGTGAAGGGGACCTGAACAAGGCTGCAGAACTTCGGTATGGCAAAATTCC
AGAACTTGAAAAAAAAATCATGCTTCTTACTGCAGAAGTAGAGAAAAAATCCGGTCTAGAAGGACAGCTCTTGCGCGAGG
AAGTGTGTGAAGAGGACATTGCGAAAATTATTTCTATGTGGACCGGAATTCCGGTATCCAAAATGATGGCAAGCGAGCAA
CAGAAATATCTGCAGCTTGAGTCAGTACTCATGCAACGTGTGGTAGGGCAGGACGAAGCAGTGCGGGTAATTTCCGACGC
GATTCGTCGTAATAAGGCAGGACTTTCTGATACGCGCCGTCCTCTTGGCAGTTTCTTATGTGTCGGTCCCACGGGGGTAG
GAAAGACAGAACTTGCACGTACGTTAGCTGATTTTCTTTTCAACGATGAGCGTGCACTGACGCGTATCGATATGAGTGAA
TACATGGAAAAACACGCGATCAGCCGACTCATTGGCGCGCCCCCGGGGTATGTGGGCTATGACGAGGGGGGACAATTGAC
AGAAGCGGTACGACGTAGACCCTACAGCGTACTTCTTTTTGATGAAGTAGAGAAAGCGCACCAGGATGTGTTTAATATAT
TCCTGCAAATACTCGACGATGGGCGCTTGACTGACGGCCAAGGAAGGGTGGTGGATTTCCGCAACACGATCATCATCATG
ACCAGCAATATCGGATCAGAGCATATTCTTTCTGCACGCGAGTCGCGCACACACACGTCGGACTTGCCTGTACCCGAGAC
ACAATCTACAGAAGAACAAACTCTACCAGAGCAGATACGGGGATTACTGCACACATACTTTCGCCCAGAATTCTTAAACC
GGATTGACGAAGTGTTAATTTTTAAGCGTCTCACACGGAAACATATTCGCCTCATCACAGACATCCAACTGCAGATGGTA
GTGGAGCGTTTGGAAAGTCGACATATAAAACTTCGTGTGCGTGACGCGGCGAAAGCCTATCTTGCGGAGCGCGGATACGA
CGACACTTTCGGAGCACGACCACTGAAGCGTGCAATCCAAACGGAATTGGAAAATGCCCTAGCGCGTGAGATTCTCAGTG
GCCGATTCAGAGGCGGCTCAACTATCGTGGTGGATATGTGTAAAGATGCTCTGTGTTTCACTGAACAAACATCCTGA

Upstream 100 bases:

>100_bases
CCTGTTCTCCACCGCGACTACTCCCGTAAGTCCCCTGCAAGCGGTAGAAAAAACATACCGATGACGAGAAAAAAGCGTTC
AAAAGACCTATAATCACGCC

Downstream 100 bases:

>100_bases
GTGCATATTCAAATATGGCAATGTCATGCCACATGTAAGAGGAGACCATCATGTTACCGTTTTACTTTTTATCTGTGGCG
ACCAACGCTGCGATAGGCTT

Product: ATP-dependent Clp protease, subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 878; Mature: 878

Protein sequence:

>878_residues
MNTDRYTVKASEALNDAISLAEAENHGQVEEEHLLHALLSQKDGIISPLIEKIGAKPDFLYDELLQCLRRKPRVTGPAAQ
TRCAPTLSKACARAERLALKNQDEYVSCEHLLLAISETDSNTARLLHSQGITSKTISAALKDIRGSKRVTSQDPESTFQC
LEKYCRDLTTLAREEKIDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEGLARRIVSGDVPESLKGKRLLSL
DLGALVAGAKFRGEFEERLKAVIEAVQKSDGGVILFIDELHTLVGAGASEGSMDASNLLKPALARGELRSIGATTLNEYR
KYIEKDAALERRFQQVYCVQPTVEDTIAILRGLQEKYEVHHGVRIKDEALVAATVLSDRYITNRFLPDKAIDLVDEAASR
LKMEIESQPVELDQVERKILQLNIEKASLLKESDPASKERLEKLEKELAGFLERRAAMQVQWQNEKGRIEESRRYKEELE
RLRIEETMFSREGDLNKAAELRYGKIPELEKKIMLLTAEVEKKSGLEGQLLREEVCEEDIAKIISMWTGIPVSKMMASEQ
QKYLQLESVLMQRVVGQDEAVRVISDAIRRNKAGLSDTRRPLGSFLCVGPTGVGKTELARTLADFLFNDERALTRIDMSE
YMEKHAISRLIGAPPGYVGYDEGGQLTEAVRRRPYSVLLFDEVEKAHQDVFNIFLQILDDGRLTDGQGRVVDFRNTIIIM
TSNIGSEHILSARESRTHTSDLPVPETQSTEEQTLPEQIRGLLHTYFRPEFLNRIDEVLIFKRLTRKHIRLITDIQLQMV
VERLESRHIKLRVRDAAKAYLAERGYDDTFGARPLKRAIQTELENALAREILSGRFRGGSTIVVDMCKDALCFTEQTS

Sequences:

>Translated_878_residues
MNTDRYTVKASEALNDAISLAEAENHGQVEEEHLLHALLSQKDGIISPLIEKIGAKPDFLYDELLQCLRRKPRVTGPAAQ
TRCAPTLSKACARAERLALKNQDEYVSCEHLLLAISETDSNTARLLHSQGITSKTISAALKDIRGSKRVTSQDPESTFQC
LEKYCRDLTTLAREEKIDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEGLARRIVSGDVPESLKGKRLLSL
DLGALVAGAKFRGEFEERLKAVIEAVQKSDGGVILFIDELHTLVGAGASEGSMDASNLLKPALARGELRSIGATTLNEYR
KYIEKDAALERRFQQVYCVQPTVEDTIAILRGLQEKYEVHHGVRIKDEALVAATVLSDRYITNRFLPDKAIDLVDEAASR
LKMEIESQPVELDQVERKILQLNIEKASLLKESDPASKERLEKLEKELAGFLERRAAMQVQWQNEKGRIEESRRYKEELE
RLRIEETMFSREGDLNKAAELRYGKIPELEKKIMLLTAEVEKKSGLEGQLLREEVCEEDIAKIISMWTGIPVSKMMASEQ
QKYLQLESVLMQRVVGQDEAVRVISDAIRRNKAGLSDTRRPLGSFLCVGPTGVGKTELARTLADFLFNDERALTRIDMSE
YMEKHAISRLIGAPPGYVGYDEGGQLTEAVRRRPYSVLLFDEVEKAHQDVFNIFLQILDDGRLTDGQGRVVDFRNTIIIM
TSNIGSEHILSARESRTHTSDLPVPETQSTEEQTLPEQIRGLLHTYFRPEFLNRIDEVLIFKRLTRKHIRLITDIQLQMV
VERLESRHIKLRVRDAAKAYLAERGYDDTFGARPLKRAIQTELENALAREILSGRFRGGSTIVVDMCKDALCFTEQTS
>Mature_878_residues
MNTDRYTVKASEALNDAISLAEAENHGQVEEEHLLHALLSQKDGIISPLIEKIGAKPDFLYDELLQCLRRKPRVTGPAAQ
TRCAPTLSKACARAERLALKNQDEYVSCEHLLLAISETDSNTARLLHSQGITSKTISAALKDIRGSKRVTSQDPESTFQC
LEKYCRDLTTLAREEKIDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEGLARRIVSGDVPESLKGKRLLSL
DLGALVAGAKFRGEFEERLKAVIEAVQKSDGGVILFIDELHTLVGAGASEGSMDASNLLKPALARGELRSIGATTLNEYR
KYIEKDAALERRFQQVYCVQPTVEDTIAILRGLQEKYEVHHGVRIKDEALVAATVLSDRYITNRFLPDKAIDLVDEAASR
LKMEIESQPVELDQVERKILQLNIEKASLLKESDPASKERLEKLEKELAGFLERRAAMQVQWQNEKGRIEESRRYKEELE
RLRIEETMFSREGDLNKAAELRYGKIPELEKKIMLLTAEVEKKSGLEGQLLREEVCEEDIAKIISMWTGIPVSKMMASEQ
QKYLQLESVLMQRVVGQDEAVRVISDAIRRNKAGLSDTRRPLGSFLCVGPTGVGKTELARTLADFLFNDERALTRIDMSE
YMEKHAISRLIGAPPGYVGYDEGGQLTEAVRRRPYSVLLFDEVEKAHQDVFNIFLQILDDGRLTDGQGRVVDFRNTIIIM
TSNIGSEHILSARESRTHTSDLPVPETQSTEEQTLPEQIRGLLHTYFRPEFLNRIDEVLIFKRLTRKHIRLITDIQLQMV
VERLESRHIKLRVRDAAKAYLAERGYDDTFGARPLKRAIQTELENALAREILSGRFRGGSTIVVDMCKDALCFTEQTS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family

Homologues:

Organism=Homo sapiens, GI13540606, Length=314, Percent_Identity=36.624203821656, Blast_Score=206, Evalue=5e-53,
Organism=Escherichia coli, GI1788943, Length=875, Percent_Identity=53.8285714285714, Blast_Score=927, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=406, Percent_Identity=41.3793103448276, Blast_Score=274, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6320464, Length=714, Percent_Identity=53.0812324929972, Blast_Score=746, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=878, Percent_Identity=41.6856492027335, Blast_Score=656, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CLPB_TREPA (O83110)

Other databases:

- EMBL:   AE000520
- PIR:   G71371
- RefSeq:   NP_218511.1
- ProteinModelPortal:   O83110
- IntAct:   O83110
- GeneID:   2611222
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0071
- NMPDR:   fig|243276.1.peg.70
- TIGR:   TP_0071
- HOGENOM:   HBG413133
- OMA:   WHLLMSI
- ProtClustDB:   CLSK218513
- BioCyc:   TPAL243276:TP_0071-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- Gene3D:   G3DSA:1.10.1780.10
- PRINTS:   PR00300
- SMART:   SM00382
- TIGRFAMs:   TIGR03346

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 98983; Mature: 98983

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00870 CLPAB_1; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTDRYTVKASEALNDAISLAEAENHGQVEEEHLLHALLSQKDGIISPLIEKIGAKPDFL
CCCCCEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHH
YDELLQCLRRKPRVTGPAAQTRCAPTLSKACARAERLALKNQDEYVSCEHLLLAISETDS
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEECCCC
NTARLLHSQGITSKTISAALKDIRGSKRVTSQDPESTFQCLEKYCRDLTTLAREEKIDPV
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
IGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEGLARRIVSGDVPESLKGKRLLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHCCCEEEEE
DLGALVAGAKFRGEFEERLKAVIEAVQKSDGGVILFIDELHTLVGAGASEGSMDASNLLK
HHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHH
PALARGELRSIGATTLNEYRKYIEKDAALERRFQQVYCVQPTVEDTIAILRGLQEKYEVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HGVRIKDEALVAATVLSDRYITNRFLPDKAIDLVDEAASRLKMEIESQPVELDQVERKIL
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
QLNIEKASLLKESDPASKERLEKLEKELAGFLERRAAMQVQWQNEKGRIEESRRYKEELE
HHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
RLRIEETMFSREGDLNKAAELRYGKIPELEKKIMLLTAEVEKKSGLEGQLLREEVCEEDI
HHHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHEEHHHHHCCCCHHHHHHHHHHHHH
AKIISMWTGIPVSKMMASEQQKYLQLESVLMQRVVGQDEAVRVISDAIRRNKAGLSDTRR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHH
PLGSFLCVGPTGVGKTELARTLADFLFNDERALTRIDMSEYMEKHAISRLIGAPPGYVGY
HHHCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DEGGQLTEAVRRRPYSVLLFDEVEKAHQDVFNIFLQILDDGRLTDGQGRVVDFRNTIIIM
CCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCEEEEE
TSNIGSEHILSARESRTHTSDLPVPETQSTEEQTLPEQIRGLLHTYFRPEFLNRIDEVLI
ECCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
FKRLTRKHIRLITDIQLQMVVERLESRHIKLRVRDAAKAYLAERGYDDTFGARPLKRAIQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHH
TELENALAREILSGRFRGGSTIVVDMCKDALCFTEQTS
HHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNTDRYTVKASEALNDAISLAEAENHGQVEEEHLLHALLSQKDGIISPLIEKIGAKPDFL
CCCCCEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHH
YDELLQCLRRKPRVTGPAAQTRCAPTLSKACARAERLALKNQDEYVSCEHLLLAISETDS
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEECCCC
NTARLLHSQGITSKTISAALKDIRGSKRVTSQDPESTFQCLEKYCRDLTTLAREEKIDPV
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
IGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVEGLARRIVSGDVPESLKGKRLLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHCCCEEEEE
DLGALVAGAKFRGEFEERLKAVIEAVQKSDGGVILFIDELHTLVGAGASEGSMDASNLLK
HHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHH
PALARGELRSIGATTLNEYRKYIEKDAALERRFQQVYCVQPTVEDTIAILRGLQEKYEVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HGVRIKDEALVAATVLSDRYITNRFLPDKAIDLVDEAASRLKMEIESQPVELDQVERKIL
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
QLNIEKASLLKESDPASKERLEKLEKELAGFLERRAAMQVQWQNEKGRIEESRRYKEELE
HHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHH
RLRIEETMFSREGDLNKAAELRYGKIPELEKKIMLLTAEVEKKSGLEGQLLREEVCEEDI
HHHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHEEHHHHHCCCCHHHHHHHHHHHHH
AKIISMWTGIPVSKMMASEQQKYLQLESVLMQRVVGQDEAVRVISDAIRRNKAGLSDTRR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHH
PLGSFLCVGPTGVGKTELARTLADFLFNDERALTRIDMSEYMEKHAISRLIGAPPGYVGY
HHHCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DEGGQLTEAVRRRPYSVLLFDEVEKAHQDVFNIFLQILDDGRLTDGQGRVVDFRNTIIIM
CCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCEEEEE
TSNIGSEHILSARESRTHTSDLPVPETQSTEEQTLPEQIRGLLHTYFRPEFLNRIDEVLI
ECCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
FKRLTRKHIRLITDIQLQMVVERLESRHIKLRVRDAAKAYLAERGYDDTFGARPLKRAIQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHH
TELENALAREILSGRFRGGSTIVVDMCKDALCFTEQTS
HHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876