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The map label for this gene is rsmE

Identifier: 189025266

GI number: 189025266

Start: 40277

End: 41077

Strand: Reverse

Name: rsmE

Synonym: TPASS_0032

Alternate gene names: 189025266

Gene position: 41077-40277 (Counterclockwise)

Preceding gene: 189025267

Following gene: 189025260

Centisome position: 3.6

GC content: 51.06

Gene sequence:

>801_bases
ATGAATATCGTTCTCTTTGAACAGGAAGAGGTAGTGCACGGTTGCGCTGTACTTTCTTTCAGGGATAGTCGATTTTGCCA
TATCAAGCGTGTGCTTAAATTGAGTGCGGGAGCCTGCTTCAAAGCAGGGATTATTAATGGGGTGAAAGGTTCTGCACGCA
TCTCCCTAGCCACAGAAAAGTATCTCGTAGCCGTTTTTGAAAAACTGGAATACGAAGATTGTGCCCTTTTCCCCCTTCAT
CTTGTCATAGGGTTCCCTCGTCCCATTCAGCTCAGGCGCATTTTACGCGACGTGTCCAGCCTCGGGATCTCCTCTATCCA
TCTTGTAGGGACGGAATTAGGGGAGCGATCTTACCTAGACTCAGGACTTGCTCACATGGAAAAAATGCACACGTACCTCA
TACGTGGCCTAGAACAGGCAGGAGGCACGAAACTTCCCCTCATTACTGTTTCGGAGTCGGTGCGCACCTTTTGCTCACAA
CACACCCACATACTCGGCGACAGCACACACCAAAAACTAATACTTGATACTAAGAACACCCTAACCGATCTAGGAAGCGC
CGCGCTGCGCGGGGATGTACTGTGGATTGCAATAGGGAGTGAGCGTGGATGGACCGAATCTGAACGTTTACTTTTCTCCG
CCATGGGATTTAGAGCAGTAGACATGGGAAGACGGACCTTGCGCACAGAGACCGCGGCCTGTGCCGCGTGCGCCGTTGTA
CTCGCCAACGCGCACGCGTGGAAAAGAAAAATCCCTCGGCCAGGCAAGAGATCTTCGCCCATAAGTCGAAAGAATCCCTA
G

Upstream 100 bases:

>100_bases
TCTATGTTGACACCTGGATGTTTGTGTTTTTTTTCGGCACCATGCTCGTTCTTTACTATCAAAGCTATCGATCATTTCTT
ACCCACACAAGAAATGACAA

Downstream 100 bases:

>100_bases
ATCCGGATCACACTCAAAAAGTAAACCAGAAAAGCCCGAGACAGGCCTGAAACAAGGAAACACAACCAAAAGATCCACAC
CGCACGCGTCAACCGAAAGC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MNIVLFEQEEVVHGCAVLSFRDSRFCHIKRVLKLSAGACFKAGIINGVKGSARISLATEKYLVAVFEKLEYEDCALFPLH
LVIGFPRPIQLRRILRDVSSLGISSIHLVGTELGERSYLDSGLAHMEKMHTYLIRGLEQAGGTKLPLITVSESVRTFCSQ
HTHILGDSTHQKLILDTKNTLTDLGSAALRGDVLWIAIGSERGWTESERLLFSAMGFRAVDMGRRTLRTETAACAACAVV
LANAHAWKRKIPRPGKRSSPISRKNP

Sequences:

>Translated_266_residues
MNIVLFEQEEVVHGCAVLSFRDSRFCHIKRVLKLSAGACFKAGIINGVKGSARISLATEKYLVAVFEKLEYEDCALFPLH
LVIGFPRPIQLRRILRDVSSLGISSIHLVGTELGERSYLDSGLAHMEKMHTYLIRGLEQAGGTKLPLITVSESVRTFCSQ
HTHILGDSTHQKLILDTKNTLTDLGSAALRGDVLWIAIGSERGWTESERLLFSAMGFRAVDMGRRTLRTETAACAACAVV
LANAHAWKRKIPRPGKRSSPISRKNP
>Mature_266_residues
MNIVLFEQEEVVHGCAVLSFRDSRFCHIKRVLKLSAGACFKAGIINGVKGSARISLATEKYLVAVFEKLEYEDCALFPLH
LVIGFPRPIQLRRILRDVSSLGISSIHLVGTELGERSYLDSGLAHMEKMHTYLIRGLEQAGGTKLPLITVSESVRTFCSQ
HTHILGDSTHQKLILDTKNTLTDLGSAALRGDVLWIAIGSERGWTESERLLFSAMGFRAVDMGRRTLRTETAACAACAVV
LANAHAWKRKIPRPGKRSSPISRKNP

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSME_TREPA (O83075)

Other databases:

- EMBL:   AE000520
- PIR:   B71375
- RefSeq:   NP_218472.1
- ProteinModelPortal:   O83075
- IntAct:   O83075
- GeneID:   2611253
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0032
- NMPDR:   fig|243276.1.peg.31
- TIGR:   TP_0032
- HOGENOM:   HBG250947
- OMA:   WQTPFLE
- ProtClustDB:   PRK11713
- BioCyc:   TPAL243276:TP_0032-MONOMER
- GO:   GO:0005737
- InterPro:   IPR006700
- TIGRFAMs:   TIGR00046

Pfam domain/function: PF04452 Methyltrans_RNA

EC number: =2.1.1.193

Molecular weight: Translated: 29380; Mature: 29380

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVLFEQEEVVHGCAVLSFRDSRFCHIKRVLKLSAGACFKAGIINGVKGSARISLATEK
CEEEEECHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEHHHH
YLVAVFEKLEYEDCALFPLHLVIGFPRPIQLRRILRDVSSLGISSIHLVGTELGERSYLD
HHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCHHHHH
SGLAHMEKMHTYLIRGLEQAGGTKLPLITVSESVRTFCSQHTHILGDSTHQKLILDTKNT
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCEECCCCCCCEEEEECHHH
LTDLGSAALRGDVLWIAIGSERGWTESERLLFSAMGFRAVDMGRRTLRTETAACAACAVV
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LANAHAWKRKIPRPGKRSSPISRKNP
HHCCHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIVLFEQEEVVHGCAVLSFRDSRFCHIKRVLKLSAGACFKAGIINGVKGSARISLATEK
CEEEEECHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEHHHH
YLVAVFEKLEYEDCALFPLHLVIGFPRPIQLRRILRDVSSLGISSIHLVGTELGERSYLD
HHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCHHHHH
SGLAHMEKMHTYLIRGLEQAGGTKLPLITVSESVRTFCSQHTHILGDSTHQKLILDTKNT
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHCCCEECCCCCCCEEEEECHHH
LTDLGSAALRGDVLWIAIGSERGWTESERLLFSAMGFRAVDMGRRTLRTETAACAACAVV
HHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LANAHAWKRKIPRPGKRSSPISRKNP
HHCCHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876