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The map label for this gene is gyrA

Identifier: 189025241

GI number: 189025241

Start: 4391

End: 6832

Strand: Direct

Name: gyrA

Synonym: TPASS_0005

Alternate gene names: 189025241

Gene position: 4391-6832 (Clockwise)

Preceding gene: 189025240

Following gene: 189025242

Centisome position: 0.39

GC content: 47.5

Gene sequence:

>2442_bases
ATGGAAGAAATTAGCACCCCAGAGGGTGGCGTTCTTGTGCCCATTTCTATAGAGACAGAAGTCAAGCGTGCTTACATAGA
CTATTCTATGTCCGTCATAGTTTCTCGTGCGCTTCCGGATGTCCGCGACGGTTTAAAGCCTGTTCACAGACGTATTCTCT
ACGCGATGGAGGAAAAAGGGCTACGCTTTTCAGGACCTACACGGAAGTGTGCCAAGATAGTGGGGGACGTTTTGGGAAGC
TTTCATCCTCATGGGGATGCGTCCGTCTATGACGCGCTAGTGCGTCTTGGGCAAGATTTTTCCCTTCGTTATCCAGTCAT
TCATCCTCAAGGAAATTTCGGGACTATCGGGGGCGACCCTCCGGCAGCGTATCGGTACACCGAAGCGAAGATGGCGCGTA
TTGCAGAATCTATGGTAGAGGACATAAAAAAGGAAACGGTTTCCTTTGTTCCCAATTTTGACGATTCTGACGTAGAGCCC
ACGGTTCTTCCTGGAAGGTTTCCTTTTCTTCTTGCGAATGGGTCCAGTGGTATTGCAGTTGGTATGACTACAAACATGCC
ACCGCATAATTTGCGTGAGATAGCCGCAGCTATCTCTGCGTACATCGAGAACCCAAATCTTTCGATTCAGGAGTTATGCG
ATTGTATCAATGGTCCTGACTTTCCCACGGGAGGCATTATCTTTGGAAAGAACGGGATTAGGCAGTCTTACGAAACAGGT
CGAGGGAAAATTGTTGTCCGTGCTCGCTTTACCATCGAGACGGATTCAAAGGGTAGGGATACCATTATTTTTACAGAAGT
TCCGTATCAAGTTAATACTACCATGCTTGTTATGCGTATTGGGGAACTTGCACGTGCGAAAGTGATCGAAGGTATTGCGA
ATGTAAACGACGAGACTTCCGATCGTACAGGGCTACGCATAGTGGTAGAGCTCAAAAAGGGTACCCCCGCACAGGTAGTA
CTCAATCACCTGTTTGCAAAGACTCCCCTGCAGTCCTCTTTTAATGTGATTAATCTTGCTTTGGTAGAGGGAAGACCTCG
AATGCTCACGCTCAAGGACCTAGTGCGCTACTTTGTAGAACACCGGGTCGATGTAGTGACTCGGCGTGCGCATTTTGAAT
TACGTAAGGCTCAGGAGCGCATACACTTGGTGCGTGCGCTGATACGTGCCTTGGATGCCATTGATAAAATCATCACGCTT
ATCCGTCATTCGCAGAACACAGAGCTTGCAAAACAGCGTTTGCGTGAACAATTTGACTTTGACAACGTGCAGGCGCAGGC
GATCGTAGATATGCAGATGAAGCGCTTGACAGGTTTGGAAGTCGAGAGTTTGCGTACGGAATTGAAAGATTTGACGGAGC
TGATTTCTTCTCTGGAGGAGTTACTTACTTCTCCCCAAAAGGTCTTGGGAGTTGTTAAGAAAGAGACGCGTGATATCGCA
GATATGTTTGGGGATGATCGGCGTACAGATATTGTGAGCAATGAAATAGAATATCTGGATGTAGAAGATTTTATCCAGAA
AGAGGAAATGGTTATTCTTATTTCCCATCTTGGTTACATTAAGCGCGTTCCAGTGTCTGCGTATAGAAATCAGAATCGGG
GAGGAAAGGGCTCAAGTTCAGCGAATCTGGCGGCTCACGATTTTATTAGCCAGATATTTACTGCATCAACACATGACTAC
GTGATGTTTGTCACGAGCCGTGGGCGGGCCTATTGGCTAAAAGTATACGGGATTCCTGAATCTGGTCGGGCGAATCGTGG
TTCGCATATTAAGTCGCTTCTCATGGTAGCGACGGACGAGGAGATCACGGCCATCGTATCTTTGAGAGAGTTTAGTAATA
AAAGTTATGTTTTTATGGCTACTGCGCGAGGTGTAGTTAAAAAGGTAACTACTGATAATTTTGTGAATGCGAAGACGCGC
GGTATTATAGCGCTTAAGCTGAGCGGAGGTGACACGCTGGTGAGCGCAGTGTTGGTGCAGGACGAAGATGAAGTAATGCT
TATTACGCGTCAGGGAAAAGCATTGCGCATGTCGGGGAGGGAGGTGCGCGAAATGGGTCGCAATTCCAGTGGGGTGATTG
GGATAAAATTGACGTCCGAGGACCTAGTGGCGGGGGTTTTGCGAGTAAGCGAACAACGGAAAGTACTGATAATGACGGAG
AATGGATATGGTAAGCGGGTCAGTTTTTCAGAATTTTCTGTACATGGGCGAGGGACTGCAGGACAGAAGATTTACACACA
AACGGATAGAAAAGGTGCTATAATAGGTGCTCTTGCTGTTCTCGATACAGATGAGTGTATGTGTATTACTGGTCAGGGAA
AAACGATTCGCGTGGACGTGTGTGCAATCAGCGTGCTGGGGCGTGGTGCGCAGGGCGTGCGTGTGTTGGATATCGAGCCA
TCGGATTTAGTAGTAGGACTTAGTTGTGTAATGCAGGGGTAA

Upstream 100 bases:

>100_bases
GTTCAAAGTCCATAGGACTGCGGTTTTTCTTGCGTGCAGCCTATGCACGACTGTGTCTCTCCTTGAACGCAGTATGGCTT
TGCGTTAGAATGCCCGCCCT

Downstream 100 bases:

>100_bases
TGGGCTCTGGGGTATATTTCTCCGTGAGTGGCTGTGTATATGTTGTGAGTATTGTGGATAATGTGCGTGCAGAAGTTGAT
GTTTCACGTGAAACGTGTGG

Product: DNA topoisomerase (ATP-hydrolyzing) subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 813; Mature: 813

Protein sequence:

>813_residues
MEEISTPEGGVLVPISIETEVKRAYIDYSMSVIVSRALPDVRDGLKPVHRRILYAMEEKGLRFSGPTRKCAKIVGDVLGS
FHPHGDASVYDALVRLGQDFSLRYPVIHPQGNFGTIGGDPPAAYRYTEAKMARIAESMVEDIKKETVSFVPNFDDSDVEP
TVLPGRFPFLLANGSSGIAVGMTTNMPPHNLREIAAAISAYIENPNLSIQELCDCINGPDFPTGGIIFGKNGIRQSYETG
RGKIVVRARFTIETDSKGRDTIIFTEVPYQVNTTMLVMRIGELARAKVIEGIANVNDETSDRTGLRIVVELKKGTPAQVV
LNHLFAKTPLQSSFNVINLALVEGRPRMLTLKDLVRYFVEHRVDVVTRRAHFELRKAQERIHLVRALIRALDAIDKIITL
IRHSQNTELAKQRLREQFDFDNVQAQAIVDMQMKRLTGLEVESLRTELKDLTELISSLEELLTSPQKVLGVVKKETRDIA
DMFGDDRRTDIVSNEIEYLDVEDFIQKEEMVILISHLGYIKRVPVSAYRNQNRGGKGSSSANLAAHDFISQIFTASTHDY
VMFVTSRGRAYWLKVYGIPESGRANRGSHIKSLLMVATDEEITAIVSLREFSNKSYVFMATARGVVKKVTTDNFVNAKTR
GIIALKLSGGDTLVSAVLVQDEDEVMLITRQGKALRMSGREVREMGRNSSGVIGIKLTSEDLVAGVLRVSEQRKVLIMTE
NGYGKRVSFSEFSVHGRGTAGQKIYTQTDRKGAIIGALAVLDTDECMCITGQGKTIRVDVCAISVLGRGAQGVRVLDIEP
SDLVVGLSCVMQG

Sequences:

>Translated_813_residues
MEEISTPEGGVLVPISIETEVKRAYIDYSMSVIVSRALPDVRDGLKPVHRRILYAMEEKGLRFSGPTRKCAKIVGDVLGS
FHPHGDASVYDALVRLGQDFSLRYPVIHPQGNFGTIGGDPPAAYRYTEAKMARIAESMVEDIKKETVSFVPNFDDSDVEP
TVLPGRFPFLLANGSSGIAVGMTTNMPPHNLREIAAAISAYIENPNLSIQELCDCINGPDFPTGGIIFGKNGIRQSYETG
RGKIVVRARFTIETDSKGRDTIIFTEVPYQVNTTMLVMRIGELARAKVIEGIANVNDETSDRTGLRIVVELKKGTPAQVV
LNHLFAKTPLQSSFNVINLALVEGRPRMLTLKDLVRYFVEHRVDVVTRRAHFELRKAQERIHLVRALIRALDAIDKIITL
IRHSQNTELAKQRLREQFDFDNVQAQAIVDMQMKRLTGLEVESLRTELKDLTELISSLEELLTSPQKVLGVVKKETRDIA
DMFGDDRRTDIVSNEIEYLDVEDFIQKEEMVILISHLGYIKRVPVSAYRNQNRGGKGSSSANLAAHDFISQIFTASTHDY
VMFVTSRGRAYWLKVYGIPESGRANRGSHIKSLLMVATDEEITAIVSLREFSNKSYVFMATARGVVKKVTTDNFVNAKTR
GIIALKLSGGDTLVSAVLVQDEDEVMLITRQGKALRMSGREVREMGRNSSGVIGIKLTSEDLVAGVLRVSEQRKVLIMTE
NGYGKRVSFSEFSVHGRGTAGQKIYTQTDRKGAIIGALAVLDTDECMCITGQGKTIRVDVCAISVLGRGAQGVRVLDIEP
SDLVVGLSCVMQG
>Mature_813_residues
MEEISTPEGGVLVPISIETEVKRAYIDYSMSVIVSRALPDVRDGLKPVHRRILYAMEEKGLRFSGPTRKCAKIVGDVLGS
FHPHGDASVYDALVRLGQDFSLRYPVIHPQGNFGTIGGDPPAAYRYTEAKMARIAESMVEDIKKETVSFVPNFDDSDVEP
TVLPGRFPFLLANGSSGIAVGMTTNMPPHNLREIAAAISAYIENPNLSIQELCDCINGPDFPTGGIIFGKNGIRQSYETG
RGKIVVRARFTIETDSKGRDTIIFTEVPYQVNTTMLVMRIGELARAKVIEGIANVNDETSDRTGLRIVVELKKGTPAQVV
LNHLFAKTPLQSSFNVINLALVEGRPRMLTLKDLVRYFVEHRVDVVTRRAHFELRKAQERIHLVRALIRALDAIDKIITL
IRHSQNTELAKQRLREQFDFDNVQAQAIVDMQMKRLTGLEVESLRTELKDLTELISSLEELLTSPQKVLGVVKKETRDIA
DMFGDDRRTDIVSNEIEYLDVEDFIQKEEMVILISHLGYIKRVPVSAYRNQNRGGKGSSSANLAAHDFISQIFTASTHDY
VMFVTSRGRAYWLKVYGIPESGRANRGSHIKSLLMVATDEEITAIVSLREFSNKSYVFMATARGVVKKVTTDNFVNAKTR
GIIALKLSGGDTLVSAVLVQDEDEVMLITRQGKALRMSGREVREMGRNSSGVIGIKLTSEDLVAGVLRVSEQRKVLIMTE
NGYGKRVSFSEFSVHGRGTAGQKIYTQTDRKGAIIGALAVLDTDECMCITGQGKTIRVDVCAISVLGRGAQGVRVLDIEP
SDLVVGLSCVMQG

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family

Homologues:

Organism=Homo sapiens, GI19913406, Length=170, Percent_Identity=30.5882352941176, Blast_Score=73, Evalue=1e-12,
Organism=Homo sapiens, GI19913408, Length=362, Percent_Identity=23.7569060773481, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1788562, Length=836, Percent_Identity=45.2153110047847, Blast_Score=723, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=765, Percent_Identity=31.2418300653595, Blast_Score=366, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI212645657, Length=427, Percent_Identity=22.9508196721311, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535065, Length=173, Percent_Identity=29.4797687861272, Blast_Score=73, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI212645845, Length=216, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=198, Percent_Identity=29.7979797979798, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRA_TREPA (O83051)

Other databases:

- EMBL:   AE000520
- PIR:   D71378
- RefSeq:   NP_218446.1
- ProteinModelPortal:   O83051
- IntAct:   O83051
- MINT:   MINT-6479025
- GeneID:   2611298
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0005
- NMPDR:   fig|243276.1.peg.5
- TIGR:   TP_0005
- HOGENOM:   HBG635183
- OMA:   ELGNDWN
- ProtClustDB:   PRK05560
- BioCyc:   TPAL243276:TP_0005-MONOMER
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013760
- Gene3D:   G3DSA:3.30.1360.40
- Gene3D:   G3DSA:3.90.199.10
- SMART:   SM00434
- TIGRFAMs:   TIGR01063

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 89928; Mature: 89928

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: ACT_SITE 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEISTPEGGVLVPISIETEVKRAYIDYSMSVIVSRALPDVRDGLKPVHRRILYAMEEKG
CCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
LRFSGPTRKCAKIVGDVLGSFHPHGDASVYDALVRLGQDFSLRYPVIHPQGNFGTIGGDP
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCEECCCCCCCCCCCCC
PAAYRYTEAKMARIAESMVEDIKKETVSFVPNFDDSDVEPTVLPGRFPFLLANGSSGIAV
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCEEEECCCCCEEE
GMTTNMPPHNLREIAAAISAYIENPNLSIQELCDCINGPDFPTGGIIFGKNGIRQSYETG
EEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCEEECCCCCHHHHHCC
RGKIVVRARFTIETDSKGRDTIIFTEVPYQVNTTMLVMRIGELARAKVIEGIANVNDETS
CCEEEEEEEEEEECCCCCCCEEEEEECCCEECCEEEEHHHHHHHHHHHHHHHHCCCCCCC
DRTGLRIVVELKKGTPAQVVLNHLFAKTPLQSSFNVINLALVEGRPRMLTLKDLVRYFVE
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHH
HRVDVVTRRAHFELRKAQERIHLVRALIRALDAIDKIITLIRHSQNTELAKQRLREQFDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
DNVQAQAIVDMQMKRLTGLEVESLRTELKDLTELISSLEELLTSPQKVLGVVKKETRDIA
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
DMFGDDRRTDIVSNEIEYLDVEDFIQKEEMVILISHLGYIKRVPVSAYRNQNRGGKGSSS
HHCCCCCCCHHHCCCCCEECHHHHHCCCCEEEEEHHCCHHHCCCHHHHCCCCCCCCCCCC
ANLAAHDFISQIFTASTHDYVMFVTSRGRAYWLKVYGIPESGRANRGSHIKSLLMVATDE
CCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHEEECCC
EITAIVSLREFSNKSYVFMATARGVVKKVTTDNFVNAKTRGIIALKLSGGDTLVSAVLVQ
HHHHHEEHHHCCCCCEEEEEECCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEEEEE
DEDEVMLITRQGKALRMSGREVREMGRNSSGVIGIKLTSEDLVAGVLRVSEQRKVLIMTE
CCCCEEEEEECCCEEECCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCCCCEEEEEEC
NGYGKRVSFSEFSVHGRGTAGQKIYTQTDRKGAIIGALAVLDTDECMCITGQGKTIRVDV
CCCCCEEECHHEEECCCCCCCCEEEECCCCCCCEEEEEEEECCCCEEEEECCCCEEEEEE
CAISVLGRGAQGVRVLDIEPSDLVVGLSCVMQG
EEEEECCCCCCCCEEEEECCCHHEEEHHHEECC
>Mature Secondary Structure
MEEISTPEGGVLVPISIETEVKRAYIDYSMSVIVSRALPDVRDGLKPVHRRILYAMEEKG
CCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
LRFSGPTRKCAKIVGDVLGSFHPHGDASVYDALVRLGQDFSLRYPVIHPQGNFGTIGGDP
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCEECCCCCCCCCCCCC
PAAYRYTEAKMARIAESMVEDIKKETVSFVPNFDDSDVEPTVLPGRFPFLLANGSSGIAV
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCEEEECCCCCEEE
GMTTNMPPHNLREIAAAISAYIENPNLSIQELCDCINGPDFPTGGIIFGKNGIRQSYETG
EEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCEEECCCCCHHHHHCC
RGKIVVRARFTIETDSKGRDTIIFTEVPYQVNTTMLVMRIGELARAKVIEGIANVNDETS
CCEEEEEEEEEEECCCCCCCEEEEEECCCEECCEEEEHHHHHHHHHHHHHHHHCCCCCCC
DRTGLRIVVELKKGTPAQVVLNHLFAKTPLQSSFNVINLALVEGRPRMLTLKDLVRYFVE
CCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHH
HRVDVVTRRAHFELRKAQERIHLVRALIRALDAIDKIITLIRHSQNTELAKQRLREQFDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
DNVQAQAIVDMQMKRLTGLEVESLRTELKDLTELISSLEELLTSPQKVLGVVKKETRDIA
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
DMFGDDRRTDIVSNEIEYLDVEDFIQKEEMVILISHLGYIKRVPVSAYRNQNRGGKGSSS
HHCCCCCCCHHHCCCCCEECHHHHHCCCCEEEEEHHCCHHHCCCHHHHCCCCCCCCCCCC
ANLAAHDFISQIFTASTHDYVMFVTSRGRAYWLKVYGIPESGRANRGSHIKSLLMVATDE
CCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHEEECCC
EITAIVSLREFSNKSYVFMATARGVVKKVTTDNFVNAKTRGIIALKLSGGDTLVSAVLVQ
HHHHHEEHHHCCCCCEEEEEECCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEEEEE
DEDEVMLITRQGKALRMSGREVREMGRNSSGVIGIKLTSEDLVAGVLRVSEQRKVLIMTE
CCCCEEEEEECCCEEECCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCCCCEEEEEEC
NGYGKRVSFSEFSVHGRGTAGQKIYTQTDRKGAIIGALAVLDTDECMCITGQGKTIRVDV
CCCCCEEECHHEEECCCCCCCCEEEECCCCCCCEEEEEEEECCCCEEEEECCCCEEEEEE
CAISVLGRGAQGVRVLDIEPSDLVVGLSCVMQG
EEEEECCCCCCCCEEEEECCCHHEEEHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876