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The map label for this gene is engD [H]

Identifier: 188587506

GI number: 188587506

Start: 3149797

End: 3150903

Strand: Reverse

Name: engD [H]

Synonym: Nther_2917

Alternate gene names: 188587506

Gene position: 3150903-3149797 (Counterclockwise)

Preceding gene: 188587507

Following gene: 188587505

Centisome position: 99.54

GC content: 34.96

Gene sequence:

>1107_bases
ATGTCTATTTTAAAATGCGGGATAATTGGTTTACCAAATGTGGGAAAATCAACTTTATTTAACGCATTAACAAATGCCGG
GGCTGCTGTTGCCAATTATGCTTTCTGTACAGTGGATCCCAACAAAGGAATAGTAGCAATTCCTGACGAACGATTAGAAC
AAGTTAAGTATTGTGCAGGTTCCCCCGAAAAGACTGAAGGTTCCATAGAATTTGTCGATATAGCTGGCTTGGTCAAAGGG
GCCAATCAAGGAGAAGGTTTGGGAAATCAATTTTTATCCCATATTCGTGAAATGGATGCATTAATTCACGTATTAAGGGG
CTTTGAAGACGAAAATGTAGGACATATCTATGGACAACCTGATATTCATGAAGATTTAGAAGTTATTAATTTAGAGCTTA
TTTTGAAGGACTTAGAAACAATTGATCGCAGACTTGAAAAAACTGAAAAAATGTTAAAAACTAACAAAAAAATATATCAT
GAAGAATTTGAAGTACTCAATTATATTAAAAAACAATTAGAACAAGGTCATAATATTAAAGATCTTAAATTGGCTGACAA
AGAGAGGAAGTTCATTGACTATCTTAATTTGCTAACTCAAAAACCCGTATTACATGTGATAAACGTTAGTGAAACAGCTT
TTTCTAATTTTGAAAAAAGACAAGCTCTTGAAAAGTATCAGCAAAAGATAGAGCAAACACAAGGAGGAGAATGTTTGGCA
ATATCGGCTAAGTTGGAGTCAGAATTGTTAGAGTTGGATCCTACTGAAAAAGATGAATTTTTAGAGGAGTTCAATTTAAC
TGATTCAGGATTAAACCGACTAATAAAAAAAGCATATCAGTTACTTGACTTAATAACTTTTTTCACAGCTAACGAAAATG
AAGCCCGGGCCTGGGCTATTAAACGAGGTACCAGAGCAGTCAAAGGGGCTGGCCAAATACATTCAGATATGGAAAAAGGT
TTTATAAAGGCTGAAGTTATCAATTGGGAGGAACTGGTAAATTGCGGAAACTTCCCCCGTGCTAAAGAAGAAGGGAAATT
GCGTTTAGAAGGTAAGGACTACATAATACAAGAGGGCGATGTGATAACTTTTAGATTCAATGTTTAA

Upstream 100 bases:

>100_bases
AAGATGAAATTGATCAGGGACTGTCTGAACTAAAGGAAAAGAATTAAATCTAAAAGGTCTAACCATTGACGGTTTAAGGT
TTAAAGGGGGTTAAACAGAA

Downstream 100 bases:

>100_bases
ACTTGAAAATCCATTTGTAATATGGTATCATCAATTGTTGTATTCCCTGCTCTATTTTTATTAATAGGGCTTTAATCCAG
AGGGAGGAGGTGAAGTTATG

Product: GTP-binding protein YchF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSILKCGIIGLPNVGKSTLFNALTNAGAAVANYAFCTVDPNKGIVAIPDERLEQVKYCAGSPEKTEGSIEFVDIAGLVKG
ANQGEGLGNQFLSHIREMDALIHVLRGFEDENVGHIYGQPDIHEDLEVINLELILKDLETIDRRLEKTEKMLKTNKKIYH
EEFEVLNYIKKQLEQGHNIKDLKLADKERKFIDYLNLLTQKPVLHVINVSETAFSNFEKRQALEKYQQKIEQTQGGECLA
ISAKLESELLELDPTEKDEFLEEFNLTDSGLNRLIKKAYQLLDLITFFTANENEARAWAIKRGTRAVKGAGQIHSDMEKG
FIKAEVINWEELVNCGNFPRAKEEGKLRLEGKDYIIQEGDVITFRFNV

Sequences:

>Translated_368_residues
MSILKCGIIGLPNVGKSTLFNALTNAGAAVANYAFCTVDPNKGIVAIPDERLEQVKYCAGSPEKTEGSIEFVDIAGLVKG
ANQGEGLGNQFLSHIREMDALIHVLRGFEDENVGHIYGQPDIHEDLEVINLELILKDLETIDRRLEKTEKMLKTNKKIYH
EEFEVLNYIKKQLEQGHNIKDLKLADKERKFIDYLNLLTQKPVLHVINVSETAFSNFEKRQALEKYQQKIEQTQGGECLA
ISAKLESELLELDPTEKDEFLEEFNLTDSGLNRLIKKAYQLLDLITFFTANENEARAWAIKRGTRAVKGAGQIHSDMEKG
FIKAEVINWEELVNCGNFPRAKEEGKLRLEGKDYIIQEGDVITFRFNV
>Mature_367_residues
SILKCGIIGLPNVGKSTLFNALTNAGAAVANYAFCTVDPNKGIVAIPDERLEQVKYCAGSPEKTEGSIEFVDIAGLVKGA
NQGEGLGNQFLSHIREMDALIHVLRGFEDENVGHIYGQPDIHEDLEVINLELILKDLETIDRRLEKTEKMLKTNKKIYHE
EFEVLNYIKKQLEQGHNIKDLKLADKERKFIDYLNLLTQKPVLHVINVSETAFSNFEKRQALEKYQQKIEQTQGGECLAI
SAKLESELLELDPTEKDEFLEEFNLTDSGLNRLIKKAYQLLDLITFFTANENEARAWAIKRGTRAVKGAGQIHSDMEKGF
IKAEVINWEELVNCGNFPRAKEEGKLRLEGKDYIIQEGDVITFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=369, Percent_Identity=40.3794037940379, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI58761502, Length=184, Percent_Identity=38.5869565217391, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI24308117, Length=106, Percent_Identity=36.7924528301887, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI111955139, Length=156, Percent_Identity=28.8461538461538, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787454, Length=365, Percent_Identity=46.5753424657534, Blast_Score=340, Evalue=7e-95,
Organism=Escherichia coli, GI1789574, Length=150, Percent_Identity=30, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17509631, Length=366, Percent_Identity=41.8032786885246, Blast_Score=281, Evalue=5e-76,
Organism=Saccharomyces cerevisiae, GI6319499, Length=371, Percent_Identity=38.544474393531, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6321773, Length=379, Percent_Identity=36.9393139841689, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321649, Length=108, Percent_Identity=43.5185185185185, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24640873, Length=372, Percent_Identity=39.7849462365591, Blast_Score=254, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24640877, Length=372, Percent_Identity=39.7849462365591, Blast_Score=254, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24640875, Length=372, Percent_Identity=39.7849462365591, Blast_Score=254, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24640879, Length=331, Percent_Identity=38.0664652567976, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24585318, Length=177, Percent_Identity=31.0734463276836, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI20129375, Length=183, Percent_Identity=31.6939890710383, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 41659; Mature: 41528

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILKCGIIGLPNVGKSTLFNALTNAGAAVANYAFCTVDPNKGIVAIPDERLEQVKYCAG
CCCEEECCCCCCCCCHHHHHHHHHHCCHHHHCCEEEEECCCCCEEECCHHHHHHHHHHCC
SPEKTEGSIEFVDIAGLVKGANQGEGLGNQFLSHIREMDALIHVLRGFEDENVGHIYGQP
CCCCCCCCEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
DIHEDLEVINLELILKDLETIDRRLEKTEKMLKTNKKIYHEEFEVLNYIKKQLEQGHNIK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DLKLADKERKFIDYLNLLTQKPVLHVINVSETAFSNFEKRQALEKYQQKIEQTQGGECLA
CCHHCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ISAKLESELLELDPTEKDEFLEEFNLTDSGLNRLIKKAYQLLDLITFFTANENEARAWAI
EEHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
KRGTRAVKGAGQIHSDMEKGFIKAEVINWEELVNCGNFPRAKEEGKLRLEGKDYIIQEGD
HHCCHHHHCCHHHHHHHHHCCCEEEEECHHHHHCCCCCCCCCCCCCEEECCCCEEEECCC
VITFRFNV
EEEEEECC
>Mature Secondary Structure 
SILKCGIIGLPNVGKSTLFNALTNAGAAVANYAFCTVDPNKGIVAIPDERLEQVKYCAG
CCEEECCCCCCCCCHHHHHHHHHHCCHHHHCCEEEEECCCCCEEECCHHHHHHHHHHCC
SPEKTEGSIEFVDIAGLVKGANQGEGLGNQFLSHIREMDALIHVLRGFEDENVGHIYGQP
CCCCCCCCEEEEEHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
DIHEDLEVINLELILKDLETIDRRLEKTEKMLKTNKKIYHEEFEVLNYIKKQLEQGHNIK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DLKLADKERKFIDYLNLLTQKPVLHVINVSETAFSNFEKRQALEKYQQKIEQTQGGECLA
CCHHCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ISAKLESELLELDPTEKDEFLEEFNLTDSGLNRLIKKAYQLLDLITFFTANENEARAWAI
EEHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
KRGTRAVKGAGQIHSDMEKGFIKAEVINWEELVNCGNFPRAKEEGKLRLEGKDYIIQEGD
HHCCHHHHCCHHHHHHHHHCCCEEEEECHHHHHCCCCCCCCCCCCCEEECCCCEEEECCC
VITFRFNV
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]