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The map label for this gene is appA [H]

Identifier: 188587496

GI number: 188587496

Start: 3139241

End: 3140965

Strand: Direct

Name: appA [H]

Synonym: Nther_2906

Alternate gene names: 188587496

Gene position: 3139241-3140965 (Clockwise)

Preceding gene: 188587481

Following gene: 188587511

Centisome position: 99.17

GC content: 38.55

Gene sequence:

>1725_bases
TTGAGAAACTCAAAATTACTAGTTCTCTTTGTTGTCTTGTTGTTTGCGAGTTCCTTTGCTTTGGCGGGTTGCGCACCTCC
TGAAGAAGCTAAAGACGAAGAACCCCAAGTCGAAGATGAAGAAAAAGAAGACCCGGATGAAGTAGAAAATCCTGCTGTTG
AAAGACCAAATGAGATTATTATTGGTGGACCTGATCTTGATGGAATCTTTAATCCGCCATTACAAAGCACCGTTTATGAC
TCCTGGATTTTGGGTATGATTTTTGACACAGTTTTAACAGTTAATGAAGATGGTGAATTAACTACAGGCCAAAGGTCAGT
TGCTGAAGATTACGAGATATCAGATGATGGTCTAGAATACACTTTTTACCTGCGTGAAGGGTGGAAGTTTCATGATGGTG
TGGAAATTACAGCCGAGGATGTGGCCTTCACTTTAGAAGTTACTGCACATCCTGAATACGACGGCCCTCGTTCTACTTGG
TCCGATAATATTGTGGGAGTAGAAGAATATCGAGAAGGTGAAACCGATGAAATTGAAGGTATCGAAATCATTGATGATTA
CACCATTCAAATCACTTCACAAGAACCCAATGCTGGTGACATTTATGATTATTCTACCTGGGTAATGCCAAGTCATTACT
ATGAATTTGACGAGTATGAGGAACTGCATGAGTTAACTGATGACCCCATGGGTAGTGGTCCTTTTAAGCTTGAAGAGTAT
AGACCCGATGAACACGCAATTCTGGTAGCAAATGAAGATTACTATCTAGGAGAGCCTGAAGTGGATAGAATCATTTACGA
AGAAATAGAATCCGAACAGCAGTTACCATTGGTTGAAACTGGTGAAGCTGATATCGTTCAAGTTTCTTCAACTCCTGAAA
ATTTAGAAATGTTACAAGATATAGATTTTCAAGAAGAATTAACTTTCTTGGATAATTCTTATAATTATATTGGAATTCAG
CACGATAATAAGCATGTAGAAAATCAAAAAGTTAGGCAAGCGTTGGCCTATGGTTTAGATATAGATGCTTTTATTGAAGG
TTTACTAGGTGATGAGTTAGGTAGAGCTATTGCTGCTCCATTTTCACCTGTAAGTTGGGCTTATCCTGAAGGTGAGTTAA
ATTACTACGAATATGATCCAGAAAAGGCAAATGAATTATTAGATGAGGCTGGTTACGAATGGGATGAAAATGAAGAGTTT
AGAGTTGATGAAGATGGCGAAAAACTTTCTCTACAATGGGACACCATAGCGGATAATGAATGGTCAGAGCACCTGACTAC
TTTAGCTTTAGAGCAATGGCCGGAAATCGGGGTTGAACTCAATATTGATAACTATGAATTTAACTCAATGGTGGACCGTC
TAGATGATGCACGTGGAGAATTCGATCTTTGGAATATGGGCTGGAGTTTGGGTACAGACCCAGATCCAAGCAATATCTTT
AGTGTAGAATACACTGGTATTGGTGAATTTAACACAGGACATTATCACAATGAAGAAGCAGAAGAGTTAATGGAAGAAGG
TATCAGAACCTTTGATCAAGACGATAGACAAGAAATATATCATGAATTAGCACATGTATTTAATGAAGATCTACCCTATA
TTTTCGTATATAGTTCCAAAGAACTCTGGTCCCAAAATGATAGGGTAGAAAACTTTGAGCCTTCAGCGTGGCAAGCATTA
AGCTGGAATGTTCACGAATGGGAAGTATCAGAGTATCAAGAATAA

Upstream 100 bases:

>100_bases
TAAAAAAACAGGAGGTGATATTTGTTAACATAATTAAATAGTTATTTGCCCATTGAACTAAAGTTAGTGAACTAAAACAA
TTATTTTGGAGGGGGATTCT

Downstream 100 bases:

>100_bases
AATAATTTTAAACTAAAACAATTAAGTTAGATAACAGTGACTAACCGCCGTCCCTCTTACTGCCGCAAATTTACTAATCC
AATACGGCTTCGTTCCAGAA

Product: extracellular solute-binding protein family 5

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MRNSKLLVLFVVLLFASSFALAGCAPPEEAKDEEPQVEDEEKEDPDEVENPAVERPNEIIIGGPDLDGIFNPPLQSTVYD
SWILGMIFDTVLTVNEDGELTTGQRSVAEDYEISDDGLEYTFYLREGWKFHDGVEITAEDVAFTLEVTAHPEYDGPRSTW
SDNIVGVEEYREGETDEIEGIEIIDDYTIQITSQEPNAGDIYDYSTWVMPSHYYEFDEYEELHELTDDPMGSGPFKLEEY
RPDEHAILVANEDYYLGEPEVDRIIYEEIESEQQLPLVETGEADIVQVSSTPENLEMLQDIDFQEELTFLDNSYNYIGIQ
HDNKHVENQKVRQALAYGLDIDAFIEGLLGDELGRAIAAPFSPVSWAYPEGELNYYEYDPEKANELLDEAGYEWDENEEF
RVDEDGEKLSLQWDTIADNEWSEHLTTLALEQWPEIGVELNIDNYEFNSMVDRLDDARGEFDLWNMGWSLGTDPDPSNIF
SVEYTGIGEFNTGHYHNEEAEELMEEGIRTFDQDDRQEIYHELAHVFNEDLPYIFVYSSKELWSQNDRVENFEPSAWQAL
SWNVHEWEVSEYQE

Sequences:

>Translated_574_residues
MRNSKLLVLFVVLLFASSFALAGCAPPEEAKDEEPQVEDEEKEDPDEVENPAVERPNEIIIGGPDLDGIFNPPLQSTVYD
SWILGMIFDTVLTVNEDGELTTGQRSVAEDYEISDDGLEYTFYLREGWKFHDGVEITAEDVAFTLEVTAHPEYDGPRSTW
SDNIVGVEEYREGETDEIEGIEIIDDYTIQITSQEPNAGDIYDYSTWVMPSHYYEFDEYEELHELTDDPMGSGPFKLEEY
RPDEHAILVANEDYYLGEPEVDRIIYEEIESEQQLPLVETGEADIVQVSSTPENLEMLQDIDFQEELTFLDNSYNYIGIQ
HDNKHVENQKVRQALAYGLDIDAFIEGLLGDELGRAIAAPFSPVSWAYPEGELNYYEYDPEKANELLDEAGYEWDENEEF
RVDEDGEKLSLQWDTIADNEWSEHLTTLALEQWPEIGVELNIDNYEFNSMVDRLDDARGEFDLWNMGWSLGTDPDPSNIF
SVEYTGIGEFNTGHYHNEEAEELMEEGIRTFDQDDRQEIYHELAHVFNEDLPYIFVYSSKELWSQNDRVENFEPSAWQAL
SWNVHEWEVSEYQE
>Mature_574_residues
MRNSKLLVLFVVLLFASSFALAGCAPPEEAKDEEPQVEDEEKEDPDEVENPAVERPNEIIIGGPDLDGIFNPPLQSTVYD
SWILGMIFDTVLTVNEDGELTTGQRSVAEDYEISDDGLEYTFYLREGWKFHDGVEITAEDVAFTLEVTAHPEYDGPRSTW
SDNIVGVEEYREGETDEIEGIEIIDDYTIQITSQEPNAGDIYDYSTWVMPSHYYEFDEYEELHELTDDPMGSGPFKLEEY
RPDEHAILVANEDYYLGEPEVDRIIYEEIESEQQLPLVETGEADIVQVSSTPENLEMLQDIDFQEELTFLDNSYNYIGIQ
HDNKHVENQKVRQALAYGLDIDAFIEGLLGDELGRAIAAPFSPVSWAYPEGELNYYEYDPEKANELLDEAGYEWDENEEF
RVDEDGEKLSLQWDTIADNEWSEHLTTLALEQWPEIGVELNIDNYEFNSMVDRLDDARGEFDLWNMGWSLGTDPDPSNIF
SVEYTGIGEFNTGHYHNEEAEELMEEGIRTFDQDDRQEIYHELAHVFNEDLPYIFVYSSKELWSQNDRVENFEPSAWQAL
SWNVHEWEVSEYQE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=495, Percent_Identity=26.4646464646465, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1787052, Length=464, Percent_Identity=22.8448275862069, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1787495, Length=469, Percent_Identity=23.454157782516, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1787551, Length=460, Percent_Identity=23.4782608695652, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 66319; Mature: 66319

Theoretical pI: Translated: 3.59; Mature: 3.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNSKLLVLFVVLLFASSFALAGCAPPEEAKDEEPQVEDEEKEDPDEVENPAVERPNEII
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEE
IGGPDLDGIFNPPLQSTVYDSWILGMIFDTVLTVNEDGELTTGQRSVAEDYEISDDGLEY
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHCCCCCCCCCCEE
TFYLREGWKFHDGVEITAEDVAFTLEVTAHPEYDGPRSTWSDNIVGVEEYREGETDEIEG
EEEEECCCEECCCCEEEECCEEEEEEEEECCCCCCCCCCCCCCCEEHHHHCCCCCCCCCC
IEIIDDYTIQITSQEPNAGDIYDYSTWVMPSHYYEFDEYEELHELTDDPMGSGPFKLEEY
EEEEECEEEEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEHHCC
RPDEHAILVANEDYYLGEPEVDRIIYEEIESEQQLPLVETGEADIVQVSSTPENLEMLQD
CCCCCEEEEECCCEECCCCHHHHHHHHHHCCCCCCCEEECCCCCEEEECCCCHHHHHHHC
IDFQEELTFLDNSYNYIGIQHDNKHVENQKVRQALAYGLDIDAFIEGLLGDELGRAIAAP
CCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
FSPVSWAYPEGELNYYEYDPEKANELLDEAGYEWDENEEFRVDEDGEKLSLQWDTIADNE
CCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEECCCCCCC
WSEHLTTLALEQWPEIGVELNIDNYEFNSMVDRLDDARGEFDLWNMGWSLGTDPDPSNIF
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEE
SVEYTGIGEFNTGHYHNEEAEELMEEGIRTFDQDDRQEIYHELAHVFNEDLPYIFVYSSK
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCH
ELWSQNDRVENFEPSAWQALSWNVHEWEVSEYQE
HHHCCCCCCCCCCCCCCEEEECCCEEECHHHCCC
>Mature Secondary Structure
MRNSKLLVLFVVLLFASSFALAGCAPPEEAKDEEPQVEDEEKEDPDEVENPAVERPNEII
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCEEE
IGGPDLDGIFNPPLQSTVYDSWILGMIFDTVLTVNEDGELTTGQRSVAEDYEISDDGLEY
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHCCCCCCCCCCEE
TFYLREGWKFHDGVEITAEDVAFTLEVTAHPEYDGPRSTWSDNIVGVEEYREGETDEIEG
EEEEECCCEECCCCEEEECCEEEEEEEEECCCCCCCCCCCCCCCEEHHHHCCCCCCCCCC
IEIIDDYTIQITSQEPNAGDIYDYSTWVMPSHYYEFDEYEELHELTDDPMGSGPFKLEEY
EEEEECEEEEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEHHCC
RPDEHAILVANEDYYLGEPEVDRIIYEEIESEQQLPLVETGEADIVQVSSTPENLEMLQD
CCCCCEEEEECCCEECCCCHHHHHHHHHHCCCCCCCEEECCCCCEEEECCCCHHHHHHHC
IDFQEELTFLDNSYNYIGIQHDNKHVENQKVRQALAYGLDIDAFIEGLLGDELGRAIAAP
CCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
FSPVSWAYPEGELNYYEYDPEKANELLDEAGYEWDENEEFRVDEDGEKLSLQWDTIADNE
CCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEECCCCCCC
WSEHLTTLALEQWPEIGVELNIDNYEFNSMVDRLDDARGEFDLWNMGWSLGTDPDPSNIF
HHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEE
SVEYTGIGEFNTGHYHNEEAEELMEEGIRTFDQDDRQEIYHELAHVFNEDLPYIFVYSSK
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCH
ELWSQNDRVENFEPSAWQALSWNVHEWEVSEYQE
HHHCCCCCCCCCCCCCCEEEECCCEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]