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The map label for this gene is glyA

Identifier: 188587448

GI number: 188587448

Start: 3089146

End: 3090384

Strand: Reverse

Name: glyA

Synonym: Nther_2858

Alternate gene names: 188587448

Gene position: 3090384-3089146 (Counterclockwise)

Preceding gene: 188587449

Following gene: 188587447

Centisome position: 97.63

GC content: 39.79

Gene sequence:

>1239_bases
ATGGAAAACGTTAAAAAGACAGATCCAACTATTTTTTCTTGGATTGAAGAAGAATGGAAAAGACAGGAGGAAGGTATTGA
ATTAATAGCTTCTGAAAACTTTGCCTCTCGGGCAGTGATGGAAGCTCAAGGTAGTGTCCTAACGAATAAATATGCAGAAG
GATATCCCGGAAGGAGATATTATGGCGGATGTCAATTTGTTGATAAAGTAGAAGAACTGGCTATCAGTAGAGTTAAAGAG
CTATTTAATGCCGATCATGCCAATGTCCAGCCCCATTCAGGAGCCTCAGCAAATATGGGGGTTTATCTGGCAGCATTGAA
GCCCGGTGATACTGTCTTGGGTATGAGCTTAGATCATGGTGGACATTTAACCCACGGAAGTCCGGTCAATATATCTGGTA
AATATTTCAATTTTCATCATTATGGTATTTTAGAGGATACAGGTAAAATTGATTTTGACAAAGTCCGGGAGTTGGCAAAA
GAACATAAACCTAAAATGATAGTAGCTGGCGCTAGTGCTTATCCACGCATTATTGATTTTGCTACTTTTCGCGAAATCGC
TGATGAGGTAGGGGCTTATCTAATGGTAGATATGGCTCACATTGCAGGGCTTGTGGCAGCGGGGCTACACCCAAACCCAG
TCCCTTATGCTGACTTTGTGACCACAACTACTCACAAAACTTTAAGGGGTCCACGTGGTGGAGTGGTTCTATGTAAAGAA
GAGTACAAAAAAGAAATTGACAAAGCCATGTTTCCAGGTCTACAGGGAGGACCTCTAATGCATGTTATTGCTTCCAAGGC
CGTCAGCTTTCAGGAAGCTTTAAGTTCTGAATTTAAAAATTATCAAAAACAAGTAATAAAAAATGCTAGCGTTTTGGCTG
ATGAACTTAATAATTTAGGATACGATTTGGTAGCAGGAGGCTCTGATAATCATCTAATGCTAGTTGATCTTCAGAAAAAA
GGTGTAACTGGTAAAAAAGCTGAACGAGTTCTGGATGATGTTCATATCACTGTCAATAAAAATGCCGTACCTAATGATCC
AGAAGGTCCTTTTGTAACAAGTGGATTGAGACTGGGGACACCTGCTGTTACTACCAGAGGTTTTGCAGAAGATGAAATCA
AAGAAGTTGCTCAGTTGCTCGATAAAGTAATTACCGGATTAGAAGATCAGGAAAATCTAGAAAAATGTAAAAAACAGGTA
ACCGACTTATGTCATAGATTCCCCTTGTATCGTCAATAA

Upstream 100 bases:

>100_bases
ACACCTTCTTAAACAGTCCCTTTGGAGGCGGTCGCCATGAAAGGCGAGTAAATAAAATTACAAAGTTAGAACAAAAATAC
TCTTAAAAGGAGGATCATTA

Downstream 100 bases:

>100_bases
TCTAGTTTTATCCCCTTTAAATTTGTGTTATTATAGATGTGATTTAAGATTGGATAAAATTAATTGGACTAAATAGGGGG
TTATAGTATGAGCAAATTAC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MENVKKTDPTIFSWIEEEWKRQEEGIELIASENFASRAVMEAQGSVLTNKYAEGYPGRRYYGGCQFVDKVEELAISRVKE
LFNADHANVQPHSGASANMGVYLAALKPGDTVLGMSLDHGGHLTHGSPVNISGKYFNFHHYGILEDTGKIDFDKVRELAK
EHKPKMIVAGASAYPRIIDFATFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPYADFVTTTTHKTLRGPRGGVVLCKE
EYKKEIDKAMFPGLQGGPLMHVIASKAVSFQEALSSEFKNYQKQVIKNASVLADELNNLGYDLVAGGSDNHLMLVDLQKK
GVTGKKAERVLDDVHITVNKNAVPNDPEGPFVTSGLRLGTPAVTTRGFAEDEIKEVAQLLDKVITGLEDQENLEKCKKQV
TDLCHRFPLYRQ

Sequences:

>Translated_412_residues
MENVKKTDPTIFSWIEEEWKRQEEGIELIASENFASRAVMEAQGSVLTNKYAEGYPGRRYYGGCQFVDKVEELAISRVKE
LFNADHANVQPHSGASANMGVYLAALKPGDTVLGMSLDHGGHLTHGSPVNISGKYFNFHHYGILEDTGKIDFDKVRELAK
EHKPKMIVAGASAYPRIIDFATFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPYADFVTTTTHKTLRGPRGGVVLCKE
EYKKEIDKAMFPGLQGGPLMHVIASKAVSFQEALSSEFKNYQKQVIKNASVLADELNNLGYDLVAGGSDNHLMLVDLQKK
GVTGKKAERVLDDVHITVNKNAVPNDPEGPFVTSGLRLGTPAVTTRGFAEDEIKEVAQLLDKVITGLEDQENLEKCKKQV
TDLCHRFPLYRQ
>Mature_412_residues
MENVKKTDPTIFSWIEEEWKRQEEGIELIASENFASRAVMEAQGSVLTNKYAEGYPGRRYYGGCQFVDKVEELAISRVKE
LFNADHANVQPHSGASANMGVYLAALKPGDTVLGMSLDHGGHLTHGSPVNISGKYFNFHHYGILEDTGKIDFDKVRELAK
EHKPKMIVAGASAYPRIIDFATFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPYADFVTTTTHKTLRGPRGGVVLCKE
EYKKEIDKAMFPGLQGGPLMHVIASKAVSFQEALSSEFKNYQKQVIKNASVLADELNNLGYDLVAGGSDNHLMLVDLQKK
GVTGKKAERVLDDVHITVNKNAVPNDPEGPFVTSGLRLGTPAVTTRGFAEDEIKEVAQLLDKVITGLEDQENLEKCKKQV
TDLCHRFPLYRQ

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=421, Percent_Identity=46.0807600950119, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI261862352, Length=421, Percent_Identity=46.0807600950119, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI261862350, Length=421, Percent_Identity=46.0807600950119, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI261862348, Length=421, Percent_Identity=46.0807600950119, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI261862346, Length=421, Percent_Identity=45.1306413301663, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI22547186, Length=405, Percent_Identity=47.4074074074074, Blast_Score=350, Evalue=9e-97,
Organism=Homo sapiens, GI22547189, Length=390, Percent_Identity=45.6410256410256, Blast_Score=323, Evalue=2e-88,
Organism=Escherichia coli, GI1788902, Length=411, Percent_Identity=60.8272506082725, Blast_Score=498, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI25144732, Length=444, Percent_Identity=45.4954954954955, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25144729, Length=444, Percent_Identity=45.4954954954955, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319739, Length=419, Percent_Identity=46.0620525059666, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323087, Length=422, Percent_Identity=43.3649289099526, Blast_Score=354, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24640005, Length=448, Percent_Identity=45.3125, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI221329721, Length=448, Percent_Identity=45.3125, Blast_Score=396, Evalue=1e-110,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_NATTJ (B2A3H6)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001918993.1
- ProteinModelPortal:   B2A3H6
- SMR:   B2A3H6
- GeneID:   6316948
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2858
- HOGENOM:   HBG301263
- OMA:   PRITSGI
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45400; Mature: 45400

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENVKKTDPTIFSWIEEEWKRQEEGIELIASENFASRAVMEAQGSVLTNKYAEGYPGRRY
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCCCC
YGGCQFVDKVEELAISRVKELFNADHANVQPHSGASANMGVYLAALKPGDTVLGMSLDHG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCC
GHLTHGSPVNISGKYFNFHHYGILEDTGKIDFDKVRELAKEHKPKMIVAGASAYPRIIDF
CCCCCCCCCCCCCEEEEEEECEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHH
ATFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPYADFVTTTTHKTLRGPRGGVVLCKE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHCCCCCCEEEEHH
EYKKEIDKAMFPGLQGGPLMHVIASKAVSFQEALSSEFKNYQKQVIKNASVLADELNNLG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YDLVAGGSDNHLMLVDLQKKGVTGKKAERVLDDVHITVNKNAVPNDPEGPFVTSGLRLGT
CEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEEECCCCCCCCCCCEEECCCCCCC
PAVTTRGFAEDEIKEVAQLLDKVITGLEDQENLEKCKKQVTDLCHRFPLYRQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MENVKKTDPTIFSWIEEEWKRQEEGIELIASENFASRAVMEAQGSVLTNKYAEGYPGRRY
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCHHHHHHHCCCCCCCC
YGGCQFVDKVEELAISRVKELFNADHANVQPHSGASANMGVYLAALKPGDTVLGMSLDHG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCC
GHLTHGSPVNISGKYFNFHHYGILEDTGKIDFDKVRELAKEHKPKMIVAGASAYPRIIDF
CCCCCCCCCCCCCEEEEEEECEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHH
ATFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPYADFVTTTTHKTLRGPRGGVVLCKE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHCCCCCCEEEEHH
EYKKEIDKAMFPGLQGGPLMHVIASKAVSFQEALSSEFKNYQKQVIKNASVLADELNNLG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YDLVAGGSDNHLMLVDLQKKGVTGKKAERVLDDVHITVNKNAVPNDPEGPFVTSGLRLGT
CEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEEECCCCCCCCCCCEEECCCCCCC
PAVTTRGFAEDEIKEVAQLLDKVITGLEDQENLEKCKKQVTDLCHRFPLYRQ
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA