Click here to switch to the map view.

The map label for this gene is gsiB [H]

Identifier: 188587381

GI number: 188587381

Start: 3013544

End: 3015127

Strand: Reverse

Name: gsiB [H]

Synonym: Nther_2790

Alternate gene names: 188587381

Gene position: 3015127-3013544 (Counterclockwise)

Preceding gene: 188587382

Following gene: 188587380

Centisome position: 95.25

GC content: 38.64

Gene sequence:

>1584_bases
ATGATTCAAAAACTAAAGAAACTCGCAATAGTAACTACCTGCTCGGCTTTAATATTGACAGCTTGTGGTGGTGAAGTTGA
TGAAGAAGCTGGTGGAAAAAACGATGAGCAAGGCAGTAAGGAAGAAGCACTCGAGGTTGAATATGAGGATAGATTGACAA
TTGGTATGGGTACCGACATGGTTACTTTTGATATCCATGACCATAACAACACCTCGACAGAAGCTGTTCATATCAATATT
TTTGATTATTTGTTTCGACAAGAAGATGGTGAAATACAGTCTGAATTAGTTTCAAAGCATGAGATTATAGATGATGAAAC
GTGGAAATTTAAATTAAAGGAAGGCGTAAAATTTCACAATGGAGACGAGTTGACTTCAAAAGATGTCCAATTCACCTTTC
ACAGGGTGATAGAAGACAGTTCTTTAACGGAACACTCGAATTACAATCAAATTTCAGAAGTGGAAGTTATTAATGATTAC
GAATTTTATATTCATACAGAAGATCCTGAACCGGCCCTACTTAACCGGATTTCTCGGATGGGTTCAGGAATTTTACCAAA
GGACTATATTGAAGAAGAGGGCTGGGATCACTTTTTAGACAGTCCTATTGGCTCAGGTCCCTATGAATTTGTTGAATGGG
AACGGGATAATCGTGTTGTACTAAATCCCTTTGAAGACTATTATGGTGACTATGATTCTCCTTGGGAAGAAGTGGTTTAC
CGAGTAATACCTGAAGATTCTACCAGGGTTTCCGAGTTGTTAACAGGGGGAGTAGATTTAGCGGTGAATGTTCCTCCTAC
TGACTGGGATAGGGTTGATGATAATCAGGGGACTCTAATATCGACAGGTGACTCCAATAGAGTTATGCTGTTAATCTTAA
ATCACGAAGAAGGACGACCTACTGAAGAGCAAAAGGTGAGGGAAGCTATTGACTATGCCATTGATAATGAAGCACTCACT
GAATCTATTCTAGACGGTCAGGGGACTCCAGTCAGAACAAGGGTTACTCCAGGTAACACCGGTTATAATGAAGAGTTATA
CGACGATTATCGTTATGATCCCGACTATTCTAGAGAACTGCTTGAAGAAGCTGGATACAGCGATGGTGTTGAACTAAAGT
TTCATGCGCCTCAAGGGCGATATTTGATGGATAGCGATGTTTCAGAAATGATTACAGGTATGTTAGCAGAAGTGGGAATA
AATGCTGATTTAAACCTGCTTGAATTCAGTCAATTTGCCGACAAGTATCTGGGAAATGAAAATGAGGATTTAATGTTCTT
AGGATTGTCTAATTCCATGTTTGATGCTGCCCATGCTCTTAGAAATTTCCATTCAGAACAAAATACTGAGAGGACCTATT
ATGAAAATGAGCGAGTAGACGAGCTGTTAGAAAAAGCCGAATCTAACATGGATTTTGAAGAACGAAAAGAACAATATCAA
GAAGTTCAAGAAATTGTTGCAGAAGAATTACCTTATGTTTACTTGTATCAACAAAAGGATAGTTACGGTATAAACAATCG
AATTGACTTCGAACCCCGTTTGGATGAAATGATTTACATTCCGGAAATTGGTAAAACAGACTAG

Upstream 100 bases:

>100_bases
AGCATTGCCTATAGGTTCTGCTATTATGGCAGGGCTCGCATTAAATTACTTAAACCAATAATAATTTAATTTTAAAGAAA
GAATTAGGAGGGGAATAATT

Downstream 100 bases:

>100_bases
AGGTGATGAATGGTGGCTAATTATATTCTAAGAAGAATACTACAACTAATCCCTGTGATTTTTATAATTTCTTTTGTTGT
ATTTTGCCTGGTATACGTTG

Product: extracellular solute-binding protein family 5

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MIQKLKKLAIVTTCSALILTACGGEVDEEAGGKNDEQGSKEEALEVEYEDRLTIGMGTDMVTFDIHDHNNTSTEAVHINI
FDYLFRQEDGEIQSELVSKHEIIDDETWKFKLKEGVKFHNGDELTSKDVQFTFHRVIEDSSLTEHSNYNQISEVEVINDY
EFYIHTEDPEPALLNRISRMGSGILPKDYIEEEGWDHFLDSPIGSGPYEFVEWERDNRVVLNPFEDYYGDYDSPWEEVVY
RVIPEDSTRVSELLTGGVDLAVNVPPTDWDRVDDNQGTLISTGDSNRVMLLILNHEEGRPTEEQKVREAIDYAIDNEALT
ESILDGQGTPVRTRVTPGNTGYNEELYDDYRYDPDYSRELLEEAGYSDGVELKFHAPQGRYLMDSDVSEMITGMLAEVGI
NADLNLLEFSQFADKYLGNENEDLMFLGLSNSMFDAAHALRNFHSEQNTERTYYENERVDELLEKAESNMDFEERKEQYQ
EVQEIVAEELPYVYLYQQKDSYGINNRIDFEPRLDEMIYIPEIGKTD

Sequences:

>Translated_527_residues
MIQKLKKLAIVTTCSALILTACGGEVDEEAGGKNDEQGSKEEALEVEYEDRLTIGMGTDMVTFDIHDHNNTSTEAVHINI
FDYLFRQEDGEIQSELVSKHEIIDDETWKFKLKEGVKFHNGDELTSKDVQFTFHRVIEDSSLTEHSNYNQISEVEVINDY
EFYIHTEDPEPALLNRISRMGSGILPKDYIEEEGWDHFLDSPIGSGPYEFVEWERDNRVVLNPFEDYYGDYDSPWEEVVY
RVIPEDSTRVSELLTGGVDLAVNVPPTDWDRVDDNQGTLISTGDSNRVMLLILNHEEGRPTEEQKVREAIDYAIDNEALT
ESILDGQGTPVRTRVTPGNTGYNEELYDDYRYDPDYSRELLEEAGYSDGVELKFHAPQGRYLMDSDVSEMITGMLAEVGI
NADLNLLEFSQFADKYLGNENEDLMFLGLSNSMFDAAHALRNFHSEQNTERTYYENERVDELLEKAESNMDFEERKEQYQ
EVQEIVAEELPYVYLYQQKDSYGINNRIDFEPRLDEMIYIPEIGKTD
>Mature_527_residues
MIQKLKKLAIVTTCSALILTACGGEVDEEAGGKNDEQGSKEEALEVEYEDRLTIGMGTDMVTFDIHDHNNTSTEAVHINI
FDYLFRQEDGEIQSELVSKHEIIDDETWKFKLKEGVKFHNGDELTSKDVQFTFHRVIEDSSLTEHSNYNQISEVEVINDY
EFYIHTEDPEPALLNRISRMGSGILPKDYIEEEGWDHFLDSPIGSGPYEFVEWERDNRVVLNPFEDYYGDYDSPWEEVVY
RVIPEDSTRVSELLTGGVDLAVNVPPTDWDRVDDNQGTLISTGDSNRVMLLILNHEEGRPTEEQKVREAIDYAIDNEALT
ESILDGQGTPVRTRVTPGNTGYNEELYDDYRYDPDYSRELLEEAGYSDGVELKFHAPQGRYLMDSDVSEMITGMLAEVGI
NADLNLLEFSQFADKYLGNENEDLMFLGLSNSMFDAAHALRNFHSEQNTERTYYENERVDELLEKAESNMDFEERKEQYQ
EVQEIVAEELPYVYLYQQKDSYGINNRIDFEPRLDEMIYIPEIGKTD

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=460, Percent_Identity=26.0869565217391, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1789966, Length=465, Percent_Identity=24.7311827956989, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787762, Length=503, Percent_Identity=23.6580516898608, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1787551, Length=438, Percent_Identity=23.972602739726, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1789887, Length=455, Percent_Identity=23.7362637362637, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI87081878, Length=456, Percent_Identity=20.8333333333333, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786256, Length=172, Percent_Identity=29.6511627906977, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789397, Length=465, Percent_Identity=20, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787495, Length=497, Percent_Identity=17.9074446680081, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60722; Mature: 60722

Theoretical pI: Translated: 3.97; Mature: 3.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKLKKLAIVTTCSALILTACGGEVDEEAGGKNDEQGSKEEALEVEYEDRLTIGMGTDM
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCHHHEEEEECCEEEEEECCCE
VTFDIHDHNNTSTEAVHINIFDYLFRQEDGEIQSELVSKHEIIDDETWKFKLKEGVKFHN
EEEEEECCCCCCCEEEEEEHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEECC
GDELTSKDVQFTFHRVIEDSSLTEHSNYNQISEVEVINDYEFYIHTEDPEPALLNRISRM
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECEEEEEECCCCCHHHHHHHHHH
GSGILPKDYIEEEGWDHFLDSPIGSGPYEFVEWERDNRVVLNPFEDYYGDYDSPWEEVVY
CCCCCCHHHHHHCCCHHHHCCCCCCCCCHHEEECCCCEEEECCHHHHCCCCCCCHHHHHH
RVIPEDSTRVSELLTGGVDLAVNVPPTDWDRVDDNQGTLISTGDSNRVMLLILNHEEGRP
HHCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCC
TEEQKVREAIDYAIDNEALTESILDGQGTPVRTRVTPGNTGYNEELYDDYRYDPDYSREL
CHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCHHHHH
LEEAGYSDGVELKFHAPQGRYLMDSDVSEMITGMLAEVGINADLNLLEFSQFADKYLGNE
HHHCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
NEDLMFLGLSNSMFDAAHALRNFHSEQNTERTYYENERVDELLEKAESNMDFEERKEQYQ
CCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EVQEIVAEELPYVYLYQQKDSYGINNRIDFEPRLDEMIYIPEIGKTD
HHHHHHHHHCCEEEEEECCCCCCCCCEECCCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MIQKLKKLAIVTTCSALILTACGGEVDEEAGGKNDEQGSKEEALEVEYEDRLTIGMGTDM
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCHHHEEEEECCEEEEEECCCE
VTFDIHDHNNTSTEAVHINIFDYLFRQEDGEIQSELVSKHEIIDDETWKFKLKEGVKFHN
EEEEEECCCCCCCEEEEEEHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCEECC
GDELTSKDVQFTFHRVIEDSSLTEHSNYNQISEVEVINDYEFYIHTEDPEPALLNRISRM
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECEEEEEECCCCCHHHHHHHHHH
GSGILPKDYIEEEGWDHFLDSPIGSGPYEFVEWERDNRVVLNPFEDYYGDYDSPWEEVVY
CCCCCCHHHHHHCCCHHHHCCCCCCCCCHHEEECCCCEEEECCHHHHCCCCCCCHHHHHH
RVIPEDSTRVSELLTGGVDLAVNVPPTDWDRVDDNQGTLISTGDSNRVMLLILNHEEGRP
HHCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCC
TEEQKVREAIDYAIDNEALTESILDGQGTPVRTRVTPGNTGYNEELYDDYRYDPDYSREL
CHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCHHHHH
LEEAGYSDGVELKFHAPQGRYLMDSDVSEMITGMLAEVGINADLNLLEFSQFADKYLGNE
HHHCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
NEDLMFLGLSNSMFDAAHALRNFHSEQNTERTYYENERVDELLEKAESNMDFEERKEQYQ
CCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EVQEIVAEELPYVYLYQQKDSYGINNRIDFEPRLDEMIYIPEIGKTD
HHHHHHHHHCCEEEEEECCCCCCCCCEECCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA