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The map label for this gene is lpdA [H]

Identifier: 188587341

GI number: 188587341

Start: 2965954

End: 2967363

Strand: Reverse

Name: lpdA [H]

Synonym: Nther_2747

Alternate gene names: 188587341

Gene position: 2967363-2965954 (Counterclockwise)

Preceding gene: 188587342

Following gene: 188587340

Centisome position: 93.74

GC content: 40.0

Gene sequence:

>1410_bases
GTGGAACAAGTCGATTTATTAATAGTAGGTAGTGGCCCAGGTGGATATGTGGCAGCTTTAAGAGGTGCTCAACTGGGAGC
TCAGGTGATGATGGTAGAAAAGGATGAAATCGGTGGAACCTGCCTTAATCGGGGATGTATTCCTACTAAAACTCTTCATA
AAAGTGCAGAACTATTTACTGAGATGAAAAAAGCCGATGAATTCGGATTTCAAATAGATAATATTAATTTGGATTATCAG
CGAGTTAGTGATAGAAAAGAAGAAATTGTGGGAAATTTAAAAGATAGTGTTAAAAAGCTGTTGAAAAAAGCCGGAGTTCA
AACTGTATTTGGTAGGTGTGAATTAAAAGCCGATAAAGAAGCAGAGGTTGACTTGCATGATGGCTCAAAAATGGAAGTAA
AGGCCAACTCTGTAATTATAGCTACAGGTTCTGAGTCTGCCACTTCTGATCTTCCTGGATCCGATCACAAAGATGTGATT
GGTACTGATGAAATACTAGAAATGAGAGAATTAGTTGATGATCTGGTGGTTATCGGTGGTGGAGTCACCGGTGTGGAATT
GGCAGGTATAATGTCGGAATTTGGGGTTAATGTTTCACTGGTAAAAAGAACACCATACATTTCACCTGTTGATGACGATA
TTGCCAAGCGTTTATTTAGCATTTTGAAGCGGGCTGGAGTTAATGTGATGACCCAAAGCCAGGTTAAAGAAATTCGTGAA
CACGATGGTGAGAAGAGTTTAGAAGTTGTAGTAGAAAAGAAAGGTAAGGAACAAAGGGTACCGGCAGATAAAGTATTGAT
TTCTAGAGGACGAAAACCTCATTTAGATGGATTAGAAGCATTGGATTTGGAAATAGGCGAAGATGGGATACAAGTAAATG
AGAAATTAGAGACAAATATACCAGGTGTTTATGCAATAGGGGATGCAGCAAGTCAAGGTGCCATGTTGGCTCATGTAGCA
CATCATCAAGGCGTTTTGGCTGCTGAAAATGCCATGGGTGAAGAAAGAACCTATGATGATAAAGCAGTTCCTAATTGTGT
ATTTACAGTCACAGAAGTTGCTTCCGTGGGAGAAAATGAAGCTTCTCTTAAAGAACAAGAAATCCCTTATAAAGTAGGAC
GCTTTCCCTTTGGTGCCAATGGAAAAGCTTTGGCAGAAGGCAAAATAGATGGTCAGGTAAAAATATTATCTCACCAAGAA
ACGGATCAGGTTTTAGGAGTCCATATTATGGGTCCTCATGCCTCTGATTTAATACAAGAAGGAACTGTAGCAGTCAAAGA
AGGACTAAAAACCGCTGATCTGGGAGAGTTGATTCATCCTCACCCTACTTTGAGCGAAACTCTTTGGGAAGCCGCTCTGG
ACTCCTTAGATATGCCCCTTCATCAGTTACCCAAAAAGAAGAAAAAGTAA

Upstream 100 bases:

>100_bases
TACGACAAGATAACTTAATAAGTTGTTTTTAGAAGTAATTGTGCCCACTGTTTATGTGGGCACAATTTACGCTTATTATT
GAAACTGCGGAGGTGTTATT

Downstream 100 bases:

>100_bases
AATTATAGCAATAGCAATATCTAACATTACATTAGATGGTGATGTAAAAAACACATCAATTTAATAAATTGACTCATTTA
TGGAAAAAATAGGCGTATCA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MEQVDLLIVGSGPGGYVAALRGAQLGAQVMMVEKDEIGGTCLNRGCIPTKTLHKSAELFTEMKKADEFGFQIDNINLDYQ
RVSDRKEEIVGNLKDSVKKLLKKAGVQTVFGRCELKADKEAEVDLHDGSKMEVKANSVIIATGSESATSDLPGSDHKDVI
GTDEILEMRELVDDLVVIGGGVTGVELAGIMSEFGVNVSLVKRTPYISPVDDDIAKRLFSILKRAGVNVMTQSQVKEIRE
HDGEKSLEVVVEKKGKEQRVPADKVLISRGRKPHLDGLEALDLEIGEDGIQVNEKLETNIPGVYAIGDAASQGAMLAHVA
HHQGVLAAENAMGEERTYDDKAVPNCVFTVTEVASVGENEASLKEQEIPYKVGRFPFGANGKALAEGKIDGQVKILSHQE
TDQVLGVHIMGPHASDLIQEGTVAVKEGLKTADLGELIHPHPTLSETLWEAALDSLDMPLHQLPKKKKK

Sequences:

>Translated_469_residues
MEQVDLLIVGSGPGGYVAALRGAQLGAQVMMVEKDEIGGTCLNRGCIPTKTLHKSAELFTEMKKADEFGFQIDNINLDYQ
RVSDRKEEIVGNLKDSVKKLLKKAGVQTVFGRCELKADKEAEVDLHDGSKMEVKANSVIIATGSESATSDLPGSDHKDVI
GTDEILEMRELVDDLVVIGGGVTGVELAGIMSEFGVNVSLVKRTPYISPVDDDIAKRLFSILKRAGVNVMTQSQVKEIRE
HDGEKSLEVVVEKKGKEQRVPADKVLISRGRKPHLDGLEALDLEIGEDGIQVNEKLETNIPGVYAIGDAASQGAMLAHVA
HHQGVLAAENAMGEERTYDDKAVPNCVFTVTEVASVGENEASLKEQEIPYKVGRFPFGANGKALAEGKIDGQVKILSHQE
TDQVLGVHIMGPHASDLIQEGTVAVKEGLKTADLGELIHPHPTLSETLWEAALDSLDMPLHQLPKKKKK
>Mature_469_residues
MEQVDLLIVGSGPGGYVAALRGAQLGAQVMMVEKDEIGGTCLNRGCIPTKTLHKSAELFTEMKKADEFGFQIDNINLDYQ
RVSDRKEEIVGNLKDSVKKLLKKAGVQTVFGRCELKADKEAEVDLHDGSKMEVKANSVIIATGSESATSDLPGSDHKDVI
GTDEILEMRELVDDLVVIGGGVTGVELAGIMSEFGVNVSLVKRTPYISPVDDDIAKRLFSILKRAGVNVMTQSQVKEIRE
HDGEKSLEVVVEKKGKEQRVPADKVLISRGRKPHLDGLEALDLEIGEDGIQVNEKLETNIPGVYAIGDAASQGAMLAHVA
HHQGVLAAENAMGEERTYDDKAVPNCVFTVTEVASVGENEASLKEQEIPYKVGRFPFGANGKALAEGKIDGQVKILSHQE
TDQVLGVHIMGPHASDLIQEGTVAVKEGLKTADLGELIHPHPTLSETLWEAALDSLDMPLHQLPKKKKK

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=461, Percent_Identity=35.5748373101952, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI50301238, Length=459, Percent_Identity=28.9760348583878, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI148277065, Length=453, Percent_Identity=25.1655629139073, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519430, Length=453, Percent_Identity=25.1655629139073, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519428, Length=453, Percent_Identity=25.1655629139073, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519426, Length=453, Percent_Identity=25.1655629139073, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI148277071, Length=453, Percent_Identity=25.1655629139073, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI291045266, Length=456, Percent_Identity=25, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI22035672, Length=461, Percent_Identity=25.8134490238612, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI291045268, Length=425, Percent_Identity=24.2352941176471, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI65787454, Length=235, Percent_Identity=27.6595744680851, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI21389617, Length=235, Percent_Identity=27.6595744680851, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI226437568, Length=235, Percent_Identity=27.6595744680851, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=31.6455696202532, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=26.6233766233766, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI87081717, Length=458, Percent_Identity=26.6375545851528, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=26.0869565217391, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32565766, Length=461, Percent_Identity=35.3579175704989, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=28, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71982272, Length=437, Percent_Identity=24.9427917620137, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=440, Percent_Identity=23.6363636363636, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71983429, Length=440, Percent_Identity=23.6363636363636, Blast_Score=96, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=474, Percent_Identity=33.3333333333333, Blast_Score=242, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=28.3870967741935, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=26.4957264957265, Blast_Score=159, Evalue=7e-40,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=35.9477124183007, Blast_Score=275, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24640553, Length=478, Percent_Identity=24.6861924686192, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24640549, Length=478, Percent_Identity=24.6861924686192, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24640551, Length=478, Percent_Identity=24.6861924686192, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=25.2083333333333, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR002218
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999 [H]

Pfam domain/function: PF01134 GIDA; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50668; Mature: 50668

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQVDLLIVGSGPGGYVAALRGAQLGAQVMMVEKDEIGGTCLNRGCIPTKTLHKSAELFT
CCCEEEEEEECCCCCCEEEECCCCCCCEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHH
EMKKADEFGFQIDNINLDYQRVSDRKEEIVGNLKDSVKKLLKKAGVQTVFGRCELKADKE
HHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCC
AEVDLHDGSKMEVKANSVIIATGSESATSDLPGSDHKDVIGTDEILEMRELVDDLVVIGG
CEEEECCCCEEEEEECEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECC
GVTGVELAGIMSEFGVNVSLVKRTPYISPVDDDIAKRLFSILKRAGVNVMTQSQVKEIRE
CCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEECHHHHHHHHH
HDGEKSLEVVVEKKGKEQRVPADKVLISRGRKPHLDGLEALDLEIGEDGIQVNEKLETNI
CCCCCHHHHHEECCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCEECCHHHCCC
PGVYAIGDAASQGAMLAHVAHHQGVLAAENAMGEERTYDDKAVPNCVFTVTEVASVGENE
CCEEEECCCCCCCHHHHHHHHHCCCEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
ASLKEQEIPYKVGRFPFGANGKALAEGKIDGQVKILSHQETDQVLGVHIMGPHASDLIQE
HHHHHHCCCHHHCCCCCCCCCCEEECCCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHC
GTVAVKEGLKTADLGELIHPHPTLSETLWEAALDSLDMPLHQLPKKKKK
CCHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure
MEQVDLLIVGSGPGGYVAALRGAQLGAQVMMVEKDEIGGTCLNRGCIPTKTLHKSAELFT
CCCEEEEEEECCCCCCEEEECCCCCCCEEEEEECCCCCCHHHCCCCCCHHHHHHHHHHHH
EMKKADEFGFQIDNINLDYQRVSDRKEEIVGNLKDSVKKLLKKAGVQTVFGRCELKADKE
HHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCC
AEVDLHDGSKMEVKANSVIIATGSESATSDLPGSDHKDVIGTDEILEMRELVDDLVVIGG
CEEEECCCCEEEEEECEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEECC
GVTGVELAGIMSEFGVNVSLVKRTPYISPVDDDIAKRLFSILKRAGVNVMTQSQVKEIRE
CCCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEECHHHHHHHHH
HDGEKSLEVVVEKKGKEQRVPADKVLISRGRKPHLDGLEALDLEIGEDGIQVNEKLETNI
CCCCCHHHHHEECCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCEECCHHHCCC
PGVYAIGDAASQGAMLAHVAHHQGVLAAENAMGEERTYDDKAVPNCVFTVTEVASVGENE
CCEEEECCCCCCCHHHHHHHHHCCCEEEHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCH
ASLKEQEIPYKVGRFPFGANGKALAEGKIDGQVKILSHQETDQVLGVHIMGPHASDLIQE
HHHHHHCCCHHHCCCCCCCCCCEEECCCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHC
GTVAVKEGLKTADLGELIHPHPTLSETLWEAALDSLDMPLHQLPKKKKK
CCHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]