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The map label for this gene is prdA [H]

Identifier: 188587309

GI number: 188587309

Start: 2929018

End: 2930676

Strand: Direct

Name: prdA [H]

Synonym: Nther_2712

Alternate gene names: 188587309

Gene position: 2929018-2930676 (Clockwise)

Preceding gene: 188587308

Following gene: 188587310

Centisome position: 92.53

GC content: 40.02

Gene sequence:

>1659_bases
ATGAATAGACATTTAGAGATACAAGGTTTTCAAATTTCCGAACTAAATTTGAATTCCCATGGTAGGACTGAAATTCAAGG
TAATAAATTATTAGTAAATAGTAATATTACTCAAGAAGTAGCAGCCAAATATCCCGAGCTAAAAGATATTAAAATGCGCG
TATTCACTAGCAAAGATGAAGATATTGAAGTGAATACCATGATGGATGTTATTCCTGTAAAGACTAAAATGGAAGATAAA
ATGGGTACAGGTACCACTTTAGAGTTGAATGGCATCACAGTATTACTGACAGGGAGAGAAGCCAGCGGCAAACAGGTAGC
GGAATTCGGTAGCACTGCTGGAAAAGTTTCAGAAAAGATCGCCTTTAATATGCCTGGTTGTCCTGATGAAGAAGATTTAA
TATTAAACCTGGATATGATAATCGAAGACGGTATAGCCATGACTAGGGACGGTCCTACAGCCTGTCACAGGGCAGCTGAT
GAAATAATACAAGAAATCAGAAATGCAATTAAAAGGGACATAAATAATACTCCCCCTCATACTACTACAACAGTCACTGA
AGGAGATACTCCGTCCCATCAAGATAAGCCGGAAGTTGTTCTAGTCAAAGAAATGATGGGTCAAGGGGGAATGCATGATA
ATTTATTGCTTCCTCTAGAACCTTGTGGAGTAACTGGTGGTAAGAGCGTAGTAGATTTGGGCAATGTCCCGGTGCTAATG
TCACCCAATGAAGTTAAAGACGGTGGCATCCATGCCATGACCTGTGTAGGACCATCAACTAAAGAAACCACTAGACATTA
TTCTAGGGATCCGTTACTCCATAAATTATATGAAGACACAGATCTTTATTTCTCTGGAGTATTGGCCGTAGGCTCACCTC
AATCAAATCATGAAAAAGAGTATGTAGCTGAACGTGTGGGCATGGCTATGGAAAAACTTCAACCTGATGGTGTAATTGTT
ATGACTGAAGGTTTCGGGAATAACCATATTGATTTTGCCAAACACATTGAAGAAGTGGGTAAAAGAGGTTTCCCGGTAGT
TGGGGTTACTTATGCAGCTAAACAAGGAGCTTTAATTATCGGAAATGAATTTATGGATGCCATGGTGGAATTAAACAAAA
GCGATAGTATGTTTGAAACGGAAGTATTAGCTGAAAACACATTGACCGATTGGGATGCTGACAGAGCAGTTACCATGCTA
AAAAACAAATTAACCAATAATACTGAATTAATAAATTCGGAAGTTCCTGTCCCCCAACAGCCTCCCGCTGTTTGGACAGA
AGCTCCTAAGGATCTATCTAATACGAAAGTAGCCCTGGTTTCAGCTGCAGGTATTCATTTAAAAGATCAAGAGCCCTTTA
ATAAGGCTGGAGATAACACTTATCGAAAAATCCCTTGGGATGTGAGTTCTGAAAATTTAATGGTAACTCACGGAGGATAT
GACCACAAGGACGTCAGACAAGATATAAATTGCATGTTCCCCATCGACAGATTAAACGAACTAGCGGATGAGGGAATGAT
TAAAGGAGGCAGTGCCAGCCATATAGGTTTTATGGGAGGTGGAGGCGATTTTGATGCCTTCAATGATAGTGTAGGTCCAG
AAATAGCACAGCAATTGAAAGAAGCTGAAGCAGGAGCAGCCATTTTCACTGCTGGATGA

Upstream 100 bases:

>100_bases
AAAATTTTTCCTGTAAAATTCTCATAATGGTTCAAGCAACAGTTAAAACTAGAAATGTTAAAACAGCTTCGCAACAGATC
ATCAAACAGGAGGATTTTAT

Downstream 100 bases:

>100_bases
GGAACTTGTCATCGCTCTGCCGTGATTGTGCAGAGAAGGGTTGAAGAAGCCGGTATACCAACAATAATGATTGCCGCTCT
GCATCCTGTAGCTAAACAGC

Product: Glycine/sarcosine/betaine reductase complex protein B alpha and beta subunits

Products: NA

Alternate protein names: D-proline reductase 45 kDa subunit; D-proline reductase 23 kDa subunit [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MNRHLEIQGFQISELNLNSHGRTEIQGNKLLVNSNITQEVAAKYPELKDIKMRVFTSKDEDIEVNTMMDVIPVKTKMEDK
MGTGTTLELNGITVLLTGREASGKQVAEFGSTAGKVSEKIAFNMPGCPDEEDLILNLDMIIEDGIAMTRDGPTACHRAAD
EIIQEIRNAIKRDINNTPPHTTTTVTEGDTPSHQDKPEVVLVKEMMGQGGMHDNLLLPLEPCGVTGGKSVVDLGNVPVLM
SPNEVKDGGIHAMTCVGPSTKETTRHYSRDPLLHKLYEDTDLYFSGVLAVGSPQSNHEKEYVAERVGMAMEKLQPDGVIV
MTEGFGNNHIDFAKHIEEVGKRGFPVVGVTYAAKQGALIIGNEFMDAMVELNKSDSMFETEVLAENTLTDWDADRAVTML
KNKLTNNTELINSEVPVPQQPPAVWTEAPKDLSNTKVALVSAAGIHLKDQEPFNKAGDNTYRKIPWDVSSENLMVTHGGY
DHKDVRQDINCMFPIDRLNELADEGMIKGGSASHIGFMGGGGDFDAFNDSVGPEIAQQLKEAEAGAAIFTAG

Sequences:

>Translated_552_residues
MNRHLEIQGFQISELNLNSHGRTEIQGNKLLVNSNITQEVAAKYPELKDIKMRVFTSKDEDIEVNTMMDVIPVKTKMEDK
MGTGTTLELNGITVLLTGREASGKQVAEFGSTAGKVSEKIAFNMPGCPDEEDLILNLDMIIEDGIAMTRDGPTACHRAAD
EIIQEIRNAIKRDINNTPPHTTTTVTEGDTPSHQDKPEVVLVKEMMGQGGMHDNLLLPLEPCGVTGGKSVVDLGNVPVLM
SPNEVKDGGIHAMTCVGPSTKETTRHYSRDPLLHKLYEDTDLYFSGVLAVGSPQSNHEKEYVAERVGMAMEKLQPDGVIV
MTEGFGNNHIDFAKHIEEVGKRGFPVVGVTYAAKQGALIIGNEFMDAMVELNKSDSMFETEVLAENTLTDWDADRAVTML
KNKLTNNTELINSEVPVPQQPPAVWTEAPKDLSNTKVALVSAAGIHLKDQEPFNKAGDNTYRKIPWDVSSENLMVTHGGY
DHKDVRQDINCMFPIDRLNELADEGMIKGGSASHIGFMGGGGDFDAFNDSVGPEIAQQLKEAEAGAAIFTAG
>Mature_552_residues
MNRHLEIQGFQISELNLNSHGRTEIQGNKLLVNSNITQEVAAKYPELKDIKMRVFTSKDEDIEVNTMMDVIPVKTKMEDK
MGTGTTLELNGITVLLTGREASGKQVAEFGSTAGKVSEKIAFNMPGCPDEEDLILNLDMIIEDGIAMTRDGPTACHRAAD
EIIQEIRNAIKRDINNTPPHTTTTVTEGDTPSHQDKPEVVLVKEMMGQGGMHDNLLLPLEPCGVTGGKSVVDLGNVPVLM
SPNEVKDGGIHAMTCVGPSTKETTRHYSRDPLLHKLYEDTDLYFSGVLAVGSPQSNHEKEYVAERVGMAMEKLQPDGVIV
MTEGFGNNHIDFAKHIEEVGKRGFPVVGVTYAAKQGALIIGNEFMDAMVELNKSDSMFETEVLAENTLTDWDADRAVTML
KNKLTNNTELINSEVPVPQQPPAVWTEAPKDLSNTKVALVSAAGIHLKDQEPFNKAGDNTYRKIPWDVSSENLMVTHGGY
DHKDVRQDINCMFPIDRLNELADEGMIKGGSASHIGFMGGGGDFDAFNDSVGPEIAQQLKEAEAGAAIFTAG

Specific function: D-proline reductase catalyzes the reductive cleavage of a C-N bond in D-proline resulting in the formation of 5- aminovalerate. The alpha subunit has been shown to bind D-proline, presumably via the pyruvoyl group [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015417 [H]

Pfam domain/function: PF09338 Gly_reductase [H]

EC number: =1.21.4.1 [H]

Molecular weight: Translated: 60176; Mature: 60176

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRHLEIQGFQISELNLNSHGRTEIQGNKLLVNSNITQEVAAKYPELKDIKMRVFTSKDE
CCCCEEEECEEEEEECCCCCCCEEECCCEEEEECCCHHHHHHCCCCCCCEEEEEEECCCC
DIEVNTMMDVIPVKTKMEDKMGTGTTLELNGITVLLTGREASGKQVAEFGSTAGKVSEKI
CEEEEEHEEEEECCHHHHHHCCCCCEEEECCEEEEEECCCCCCHHHHHHCCHHHHHHHHE
AFNMPGCPDEEDLILNLDMIIEDGIAMTRDGPTACHRAADEIIQEIRNAIKRDINNTPPH
EECCCCCCCCCCEEEEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TTTTVTEGDTPSHQDKPEVVLVKEMMGQGGMHDNLLLPLEPCGVTGGKSVVDLGNVPVLM
CEEEEECCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEEECCCCCCCCCCEEECCCCCEEE
SPNEVKDGGIHAMTCVGPSTKETTRHYSRDPLLHKLYEDTDLYFSGVLAVGSPQSNHEKE
CCCCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCEEEEEEEECCCCCCCHHH
YVAERVGMAMEKLQPDGVIVMTEGFGNNHIDFAKHIEEVGKRGFPVVGVTYAAKQGALII
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEE
GNEFMDAMVELNKSDSMFETEVLAENTLTDWDADRAVTMLKNKLTNNTELINSEVPVPQQ
CHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHEECCCCCCCCC
PPAVWTEAPKDLSNTKVALVSAAGIHLKDQEPFNKAGDNTYRKIPWDVSSENLMVTHGGY
CCCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCEECCCCCCCCCEEEEECCC
DHKDVRQDINCMFPIDRLNELADEGMIKGGSASHIGFMGGGGDFDAFNDSVGPEIAQQLK
CHHHHHHCCCEEECHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHH
EAEAGAAIFTAG
HHHCCCEEEECC
>Mature Secondary Structure
MNRHLEIQGFQISELNLNSHGRTEIQGNKLLVNSNITQEVAAKYPELKDIKMRVFTSKDE
CCCCEEEECEEEEEECCCCCCCEEECCCEEEEECCCHHHHHHCCCCCCCEEEEEEECCCC
DIEVNTMMDVIPVKTKMEDKMGTGTTLELNGITVLLTGREASGKQVAEFGSTAGKVSEKI
CEEEEEHEEEEECCHHHHHHCCCCCEEEECCEEEEEECCCCCCHHHHHHCCHHHHHHHHE
AFNMPGCPDEEDLILNLDMIIEDGIAMTRDGPTACHRAADEIIQEIRNAIKRDINNTPPH
EECCCCCCCCCCEEEEEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TTTTVTEGDTPSHQDKPEVVLVKEMMGQGGMHDNLLLPLEPCGVTGGKSVVDLGNVPVLM
CEEEEECCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEEECCCCCCCCCCEEECCCCCEEE
SPNEVKDGGIHAMTCVGPSTKETTRHYSRDPLLHKLYEDTDLYFSGVLAVGSPQSNHEKE
CCCCCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCEEEEEEEECCCCCCCHHH
YVAERVGMAMEKLQPDGVIVMTEGFGNNHIDFAKHIEEVGKRGFPVVGVTYAAKQGALII
HHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEE
GNEFMDAMVELNKSDSMFETEVLAENTLTDWDADRAVTMLKNKLTNNTELINSEVPVPQQ
CHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHEECCCCCCCCC
PPAVWTEAPKDLSNTKVALVSAAGIHLKDQEPFNKAGDNTYRKIPWDVSSENLMVTHGGY
CCCCCCCCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCEECCCCCCCCCEEEEECCC
DHKDVRQDINCMFPIDRLNELADEGMIKGGSASHIGFMGGGGDFDAFNDSVGPEIAQQLK
CHHHHHHCCCEEECHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHH
EAEAGAAIFTAG
HHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10085076 [H]