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The map label for this gene is mleN [H]

Identifier: 188587277

GI number: 188587277

Start: 2887603

End: 2888931

Strand: Reverse

Name: mleN [H]

Synonym: Nther_2679

Alternate gene names: 188587277

Gene position: 2888931-2887603 (Counterclockwise)

Preceding gene: 188587279

Following gene: 188587276

Centisome position: 91.26

GC content: 39.28

Gene sequence:

>1329_bases
ATGAAGAACAATTCACTGAATCAAGTAGAACCAAAAGGAAAAGCACTAATACCTTTATTTGTGTTTTTATTTCTCTATTT
AGGTGTTGGTCTGTGGCTGACTTACTTAGGCGTAGAAATGGCATTTTACGAATTTCCTGCGCCCATTGCTGCTCTAATAG
GAGTGGTAGTAGCTGTTATAATGGCAAAAGGAACTATCGAGGAAAGATTGCAAACTTTTATCAAGGGAGCCGGTGAAGAA
GGAATAATAGTAATGTGTATGATCTATCTACTAGCCGGTGCTTTTTCTGCTGTGGTTGAAGGTATGGGAGGTGTAGAATC
TACAGTCAATCTTGGGCTCACTCTGGTTCCACCCAGATTAATCTTACCTGGACTTTTTATCATTAGTGCTTTTATATCAC
TGGCAATGGGTACTTCCATGGGTACTATTGGAGCTGTGGCTCCTATTGCCATTGGTCTTTCAGAAGAAGTAGGATTTTCT
CTACCTCTAATTATGGGAATTGTAATTGGAGGCGCCATGTTTGGAGATAATTTGTCCATTATTTCTGATACTACTATTGC
TGCAACTCAAACTCAAGGAGTTAAAATGAGGGACAAGTTTAAAATGAACTTTATGATTGTGCTTCCTGCAGCCCTAATCA
CAATAATTATCTTATTATTTGTGGGAGATGGAGGAATCATTGAAGAAGATTATAACTTCCAATTAATTAAAGTGCTGCCT
TATATCTTTGTTCTTGTTTTTGCTTTATTAGGTACAAATGTCTTTATAGTTTTATTTACAGGCCTAGTTTTGGCAGGTCT
TATAGGAATCGCTTATGGTGATTTCACACCTTTAGAACTTACTCAAGTGTCCTATGAAGGCTTTACAGGAATGAGTGAAG
TATTCTTTTTATCCATGTTTGCCGGTGGTCTGGCGGCCATGATACGCAAAGAAGGTGGACTTGCCTATTTAGTTGAGCAA
ATTTCTCGGCGAATTAAAACCAAAAAAGGTGCAGAGCTGGGTATAGGAGCTTTGGCCAGTGCTGCTGATATATGCACGGC
GAATAATACTGTAGCCATTATCGTCAGCGGTCCTTTGGCCCAAAATATAGCTCAGGAAAATGGCGTAGATATGAGAAGGA
GTGCCAGTCTTTTAGATATATTCGCAGCCAGTTTCCAGGGGATGCTTCCCTATGGTGCCCAAATTCTATTGGCTGGTTCT
TTGGCTGGAATTTCACCACTAAGTATTATTCCAAATCTTCACTATAACTTCTTAATGCTGGCCTGTGTCCTGATAGCAAT
CTATTTAGGGATACCAAAACTAGCTACTGAAAAAACAACAGACAACTAA

Upstream 100 bases:

>100_bases
CAATTAAACATTATTATGCGTGTTTGAAATTGAAATATTACAGTTATCAAATTATAGTTTAATACGGCCAACTATTAAAA
ATCGAAAGGAGGAAGAAAAA

Downstream 100 bases:

>100_bases
CATTTAAGTGAAATTGGAATTTAAATTATCAACATTTAACTTTAAGACAACCTTTTAATTAAATTTTTACGAATAACACC
TTTTGATAAAAACCATGTTA

Product: Na+/H+ antiporter NhaC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKNNSLNQVEPKGKALIPLFVFLFLYLGVGLWLTYLGVEMAFYEFPAPIAALIGVVVAVIMAKGTIEERLQTFIKGAGEE
GIIVMCMIYLLAGAFSAVVEGMGGVESTVNLGLTLVPPRLILPGLFIISAFISLAMGTSMGTIGAVAPIAIGLSEEVGFS
LPLIMGIVIGGAMFGDNLSIISDTTIAATQTQGVKMRDKFKMNFMIVLPAALITIIILLFVGDGGIIEEDYNFQLIKVLP
YIFVLVFALLGTNVFIVLFTGLVLAGLIGIAYGDFTPLELTQVSYEGFTGMSEVFFLSMFAGGLAAMIRKEGGLAYLVEQ
ISRRIKTKKGAELGIGALASAADICTANNTVAIIVSGPLAQNIAQENGVDMRRSASLLDIFAASFQGMLPYGAQILLAGS
LAGISPLSIIPNLHYNFLMLACVLIAIYLGIPKLATEKTTDN

Sequences:

>Translated_442_residues
MKNNSLNQVEPKGKALIPLFVFLFLYLGVGLWLTYLGVEMAFYEFPAPIAALIGVVVAVIMAKGTIEERLQTFIKGAGEE
GIIVMCMIYLLAGAFSAVVEGMGGVESTVNLGLTLVPPRLILPGLFIISAFISLAMGTSMGTIGAVAPIAIGLSEEVGFS
LPLIMGIVIGGAMFGDNLSIISDTTIAATQTQGVKMRDKFKMNFMIVLPAALITIIILLFVGDGGIIEEDYNFQLIKVLP
YIFVLVFALLGTNVFIVLFTGLVLAGLIGIAYGDFTPLELTQVSYEGFTGMSEVFFLSMFAGGLAAMIRKEGGLAYLVEQ
ISRRIKTKKGAELGIGALASAADICTANNTVAIIVSGPLAQNIAQENGVDMRRSASLLDIFAASFQGMLPYGAQILLAGS
LAGISPLSIIPNLHYNFLMLACVLIAIYLGIPKLATEKTTDN
>Mature_442_residues
MKNNSLNQVEPKGKALIPLFVFLFLYLGVGLWLTYLGVEMAFYEFPAPIAALIGVVVAVIMAKGTIEERLQTFIKGAGEE
GIIVMCMIYLLAGAFSAVVEGMGGVESTVNLGLTLVPPRLILPGLFIISAFISLAMGTSMGTIGAVAPIAIGLSEEVGFS
LPLIMGIVIGGAMFGDNLSIISDTTIAATQTQGVKMRDKFKMNFMIVLPAALITIIILLFVGDGGIIEEDYNFQLIKVLP
YIFVLVFALLGTNVFIVLFTGLVLAGLIGIAYGDFTPLELTQVSYEGFTGMSEVFFLSMFAGGLAAMIRKEGGLAYLVEQ
ISRRIKTKKGAELGIGALASAADICTANNTVAIIVSGPLAQNIAQENGVDMRRSASLLDIFAASFQGMLPYGAQILLAGS
LAGISPLSIIPNLHYNFLMLACVLIAIYLGIPKLATEKTTDN

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 46795; Mature: 46795

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNSLNQVEPKGKALIPLFVFLFLYLGVGLWLTYLGVEMAFYEFPAPIAALIGVVVAVI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MAKGTIEERLQTFIKGAGEEGIIVMCMIYLLAGAFSAVVEGMGGVESTVNLGLTLVPPRL
HHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEECCHHH
ILPGLFIISAFISLAMGTSMGTIGAVAPIAIGLSEEVGFSLPLIMGIVIGGAMFGDNLSI
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCCCCCEE
ISDTTIAATQTQGVKMRDKFKMNFMIVLPAALITIIILLFVGDGGIIEEDYNFQLIKVLP
EECCEEEEECCCCCEEHHHHCCCEEEEHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHH
YIFVLVFALLGTNVFIVLFTGLVLAGLIGIAYGDFTPLELTQVSYEGFTGMSEVFFLSMF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHH
AGGLAAMIRKEGGLAYLVEQISRRIKTKKGAELGIGALASAADICTANNTVAIIVSGPLA
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCEEEEEEECHHH
QNIAQENGVDMRRSASLLDIFAASFQGMLPYGAQILLAGSLAGISPLSIIPNLHYNFLML
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHCCCCHHHHHHH
ACVLIAIYLGIPKLATEKTTDN
HHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKNNSLNQVEPKGKALIPLFVFLFLYLGVGLWLTYLGVEMAFYEFPAPIAALIGVVVAVI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MAKGTIEERLQTFIKGAGEEGIIVMCMIYLLAGAFSAVVEGMGGVESTVNLGLTLVPPRL
HHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEECCHHH
ILPGLFIISAFISLAMGTSMGTIGAVAPIAIGLSEEVGFSLPLIMGIVIGGAMFGDNLSI
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCCCCCEE
ISDTTIAATQTQGVKMRDKFKMNFMIVLPAALITIIILLFVGDGGIIEEDYNFQLIKVLP
EECCEEEEECCCCCEEHHHHCCCEEEEHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHH
YIFVLVFALLGTNVFIVLFTGLVLAGLIGIAYGDFTPLELTQVSYEGFTGMSEVFFLSMF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHH
AGGLAAMIRKEGGLAYLVEQISRRIKTKKGAELGIGALASAADICTANNTVAIIVSGPLA
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEECCCEEEEEEECHHH
QNIAQENGVDMRRSASLLDIFAASFQGMLPYGAQILLAGSLAGISPLSIIPNLHYNFLML
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHCCCCHHHHHHH
ACVLIAIYLGIPKLATEKTTDN
HHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]