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The map label for this gene is cwlH [H]

Identifier: 188587146

GI number: 188587146

Start: 2738184

End: 2738891

Strand: Reverse

Name: cwlH [H]

Synonym: Nther_2544

Alternate gene names: 188587146

Gene position: 2738891-2738184 (Counterclockwise)

Preceding gene: 188587147

Following gene: 188587144

Centisome position: 86.52

GC content: 46.75

Gene sequence:

>708_bases
GTGCAGATCAATCAGAAACTTATAGACTACAACTACTCCAGTAGACAGAATCAAACCATTTCGTACCTGGTGATCCACGA
TACCGGAAACTCCCGGCCGGGCGCCGATGCCAAAGCCCATTACCACTACTTCAATGCCGGTAACAGAAGTGCCTCGGCCC
ATTATTTTGTGGACGATACCCAGATCTTGCAGCTGGTAGAAGATCATCACGCCTCCTGGCACTGCGGTGACGGCAGGGGG
CGGTACGGAATTACCAATAGTAATTCCATAGGGATAGAGATATGCATCAACTCCGATGGGAACTATGCCAAAGCAGTAGA
ACAGACCATAAAACTGACTGCATACCTGGCAGAAAAGTACACCATCCCCCATAACAAGATAGTAAGACACTACGATGTCA
GCAGAAAGATCTGTCCTGGGACTATGAGCCGGGACAACTGGGCCCTGTGGTATGAGCTTAAAGATGATGTATTAGCACTC
AGAAAGCAGAATTCACACAGTGAGGATGCATCTGGAAAGCTAAACTCCCAAAGTGAAGTTGAACCCGAAACCAATAATCT
TAAGAGATCTATCCAGATCCTAACTGAAGCGGGTATCATCAATTCACCTGAGTACTGGGACCAACATGCCCGAAAGGGTG
AGACAATTAAAGGGCAGTATGCGGCTTTCTTGATTGAGAGGGTGGCACAAAAGTTAAAAACAAAATAG

Upstream 100 bases:

>100_bases
TATTACAGAGCTAAGCCGGGTCATCGAACTGGAACGGGGAAAGCTCATGGGAAAACCATAGAGAATAGAGATAATAAGGT
GATAGTAGGGAGGGGAAGAG

Downstream 100 bases:

>100_bases
AAAGGTGTTAATACAAAAGAGCCGTATCACAATCCATATACGGCTCTTTTGTATAGATCAGCAAATCTACGTTATTTGGA
TTTAATTCAATTGTATTTTC

Product: N-acetylmuramoyl-L-alanine amidase family 2

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MQINQKLIDYNYSSRQNQTISYLVIHDTGNSRPGADAKAHYHYFNAGNRSASAHYFVDDTQILQLVEDHHASWHCGDGRG
RYGITNSNSIGIEICINSDGNYAKAVEQTIKLTAYLAEKYTIPHNKIVRHYDVSRKICPGTMSRDNWALWYELKDDVLAL
RKQNSHSEDASGKLNSQSEVEPETNNLKRSIQILTEAGIINSPEYWDQHARKGETIKGQYAAFLIERVAQKLKTK

Sequences:

>Translated_235_residues
MQINQKLIDYNYSSRQNQTISYLVIHDTGNSRPGADAKAHYHYFNAGNRSASAHYFVDDTQILQLVEDHHASWHCGDGRG
RYGITNSNSIGIEICINSDGNYAKAVEQTIKLTAYLAEKYTIPHNKIVRHYDVSRKICPGTMSRDNWALWYELKDDVLAL
RKQNSHSEDASGKLNSQSEVEPETNNLKRSIQILTEAGIINSPEYWDQHARKGETIKGQYAAFLIERVAQKLKTK
>Mature_235_residues
MQINQKLIDYNYSSRQNQTISYLVIHDTGNSRPGADAKAHYHYFNAGNRSASAHYFVDDTQILQLVEDHHASWHCGDGRG
RYGITNSNSIGIEICINSDGNYAKAVEQTIKLTAYLAEKYTIPHNKIVRHYDVSRKICPGTMSRDNWALWYELKDDVLAL
RKQNSHSEDASGKLNSQSEVEPETNNLKRSIQILTEAGIINSPEYWDQHARKGETIKGQYAAFLIERVAQKLKTK

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26745; Mature: 26745

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQINQKLIDYNYSSRQNQTISYLVIHDTGNSRPGADAKAHYHYFNAGNRSASAHYFVDDT
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEEECHH
QILQLVEDHHASWHCGDGRGRYGITNSNSIGIEICINSDGNYAKAVEQTIKLTAYLAEKY
HHHHHHHHCCCCEEECCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHC
TIPHNKIVRHYDVSRKICPGTMSRDNWALWYELKDDVLALRKQNSHSEDASGKLNSQSEV
CCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCCCCCCCCCCCCCCCC
EPETNNLKRSIQILTEAGIINSPEYWDQHARKGETIKGQYAAFLIERVAQKLKTK
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQINQKLIDYNYSSRQNQTISYLVIHDTGNSRPGADAKAHYHYFNAGNRSASAHYFVDDT
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEEECHH
QILQLVEDHHASWHCGDGRGRYGITNSNSIGIEICINSDGNYAKAVEQTIKLTAYLAEKY
HHHHHHHHCCCCEEECCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHC
TIPHNKIVRHYDVSRKICPGTMSRDNWALWYELKDDVLALRKQNSHSEDASGKLNSQSEV
CCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCCCCCCCCCCCCCCCC
EPETNNLKRSIQILTEAGIINSPEYWDQHARKGETIKGQYAAFLIERVAQKLKTK
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]