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The map label for this gene is asnH [H]

Identifier: 188587136

GI number: 188587136

Start: 2727675

End: 2729498

Strand: Reverse

Name: asnH [H]

Synonym: Nther_2533

Alternate gene names: 188587136

Gene position: 2729498-2727675 (Counterclockwise)

Preceding gene: 188587137

Following gene: 188587135

Centisome position: 86.22

GC content: 26.59

Gene sequence:

>1824_bases
ATGAGTAAGTTAATAGGCTTATACTATATGGACAAGTATGAAAATTTAAAGTCTAAATTACGACATTTAAAAAAAGATAT
AAATGTTCCCAATGCTAGTTTTATGGAATGCTGGTCTCATGAAAATTTAGGGGTTGTTCGAGTACATCATGGGGTGATTA
ATAGTGAAGAGCAGCCAATTTTTAATGAGGATGGTACTAAATGTATTTTTATGATTGGTGAAGTTTTTGGTTATCACAAT
GAGAAAAAAAATTTAATCCAAAAAGGTCATAAATTTAAGTTCGATAATGATGCTGAATATTGTCTACATCTTTTTGAGGA
ATACGGAGAAGGTAGTTTTATTAAATTAAATGGTTCATTTAATTTAATAATATATGATTTTTTCGAAAAAAAGCTGATAA
TAGTTAACGATAGATTTGCATATAAACCGATGTTTTTTTGTAAAAAAGATAATTTAGTAGCTTTTAGTAGCAGAATTAAG
CCGTTATTAAATTTTAGTGACAAAAAATATAATATGTCTGCTATATATGACTTTTTTGGTTTTCAGAAAATTTTTGGTGA
TAAAACTTATTATAAAGATATATATTCATTGGGAAACGCAGAGTATTTAATTATTGACCATAAAGGATACATTACGAAAA
GAAAATACTGGGAAATATCTCATAACATAGTACATCGTTCAGAAGATGAGTATGTGAAAATATTGTCTGGTAAATTAGTA
AAAGCAATGGAACGCAGAACTAGTGACAAAAACAAAAAAGGTATCCTATTAAGTGGCGGATTGGATTCTAGAGCAATTTT
AGCAGGAGATCATAAAAATAAAATAAAATATGGCTTCACGATAGGAGATTTTTATAACAAAGAAGTGGAAATATCGAATA
AGGTAGCAGACTCACAAGATATGGAGTTTTATTTTTTGCAAAGAAACAAAGAGTATTATATTAATATATTAGAAAAAGCA
GTTGAAATAGGTGAAGGAATGTATAGGTTTGATCATGCACATTTTTTGAATTATACTAATAAATTGAAATCTAACGTTGA
TGTTGTTTTTACAGGGGGACTTATAGAACAATTATGGCAGGGCTCTAAGTTTTTTTACTATAACTTACAAAATAAAATTA
AAACACCATATTTATTAGATACAAATAGCGATATTCTAGAGTTTATTATTTACAAATATGGTGTTAAATTGAATAATTAT
GAAGTCTTTAAAGGGTTTAATAAACTAGAATTTCAAGACAATCTTGAAAAAAGCTTAGCTGACATTTTTTCAGAAAATAT
AAATGTTAATTTGGCAACTGATTTTATATTTTATAATAATCGTAATCCAGCTTATCTAAATCAATTATGTTTGGACGAAT
ATATTGATCATAGACTATTAGATGATACTGAACTGATTGATTTTTTATTGACAGTACCTGTTGAATTTAGGGCTAATGGA
CATATTTTAAGAAAGTCATTGAAGTATCTATCGAAAGAACTAACCAGGATTCCAACTTCTAACTATGGTATGCCAGTTTA
TTGTAATCCTTATTTTGAATTCTTAGTATCGATTTTTTATAAATCTTACCACAAACTAAAATTAAAAAGATATAATAAGT
ACTATACTAATCATTCGTGGCCTAATAGAGGACAACTGTTAAGAGATAATAAGAAATTCCAAATGAGCGTTTATAATACC
ATTACGAAATTAGATGATGAACTATTTGATAAGAAAACTTTAAAAAATTTATTTCATGATCATATAAATTATAAAAAAAA
TAACTATGAAATTTTAGCTTTGTTATTGACTTTTGGAAAGTGGCAAGAAATGACTAATAATTGA

Upstream 100 bases:

>100_bases
TGGAGCTATCTATACATTGCTGGGATATATTTTTAAATGCAAAGAAATAGAATACTTTTTCCGAATTGTAAAAGATAAAC
TTTTTTCTGGAGGATAAATA

Downstream 100 bases:

>100_bases
GGGTGTAAATAATGTTTATTTTGGGCAGTTGGATAATTACCATATATTTATCTTTATCTACTATTTATTGGTTGCCTGGA
ATATCTCCTGAAATATTAGA

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 607; Mature: 606

Protein sequence:

>607_residues
MSKLIGLYYMDKYENLKSKLRHLKKDINVPNASFMECWSHENLGVVRVHHGVINSEEQPIFNEDGTKCIFMIGEVFGYHN
EKKNLIQKGHKFKFDNDAEYCLHLFEEYGEGSFIKLNGSFNLIIYDFFEKKLIIVNDRFAYKPMFFCKKDNLVAFSSRIK
PLLNFSDKKYNMSAIYDFFGFQKIFGDKTYYKDIYSLGNAEYLIIDHKGYITKRKYWEISHNIVHRSEDEYVKILSGKLV
KAMERRTSDKNKKGILLSGGLDSRAILAGDHKNKIKYGFTIGDFYNKEVEISNKVADSQDMEFYFLQRNKEYYINILEKA
VEIGEGMYRFDHAHFLNYTNKLKSNVDVVFTGGLIEQLWQGSKFFYYNLQNKIKTPYLLDTNSDILEFIIYKYGVKLNNY
EVFKGFNKLEFQDNLEKSLADIFSENINVNLATDFIFYNNRNPAYLNQLCLDEYIDHRLLDDTELIDFLLTVPVEFRANG
HILRKSLKYLSKELTRIPTSNYGMPVYCNPYFEFLVSIFYKSYHKLKLKRYNKYYTNHSWPNRGQLLRDNKKFQMSVYNT
ITKLDDELFDKKTLKNLFHDHINYKKNNYEILALLLTFGKWQEMTNN

Sequences:

>Translated_607_residues
MSKLIGLYYMDKYENLKSKLRHLKKDINVPNASFMECWSHENLGVVRVHHGVINSEEQPIFNEDGTKCIFMIGEVFGYHN
EKKNLIQKGHKFKFDNDAEYCLHLFEEYGEGSFIKLNGSFNLIIYDFFEKKLIIVNDRFAYKPMFFCKKDNLVAFSSRIK
PLLNFSDKKYNMSAIYDFFGFQKIFGDKTYYKDIYSLGNAEYLIIDHKGYITKRKYWEISHNIVHRSEDEYVKILSGKLV
KAMERRTSDKNKKGILLSGGLDSRAILAGDHKNKIKYGFTIGDFYNKEVEISNKVADSQDMEFYFLQRNKEYYINILEKA
VEIGEGMYRFDHAHFLNYTNKLKSNVDVVFTGGLIEQLWQGSKFFYYNLQNKIKTPYLLDTNSDILEFIIYKYGVKLNNY
EVFKGFNKLEFQDNLEKSLADIFSENINVNLATDFIFYNNRNPAYLNQLCLDEYIDHRLLDDTELIDFLLTVPVEFRANG
HILRKSLKYLSKELTRIPTSNYGMPVYCNPYFEFLVSIFYKSYHKLKLKRYNKYYTNHSWPNRGQLLRDNKKFQMSVYNT
ITKLDDELFDKKTLKNLFHDHINYKKNNYEILALLLTFGKWQEMTNN
>Mature_606_residues
SKLIGLYYMDKYENLKSKLRHLKKDINVPNASFMECWSHENLGVVRVHHGVINSEEQPIFNEDGTKCIFMIGEVFGYHNE
KKNLIQKGHKFKFDNDAEYCLHLFEEYGEGSFIKLNGSFNLIIYDFFEKKLIIVNDRFAYKPMFFCKKDNLVAFSSRIKP
LLNFSDKKYNMSAIYDFFGFQKIFGDKTYYKDIYSLGNAEYLIIDHKGYITKRKYWEISHNIVHRSEDEYVKILSGKLVK
AMERRTSDKNKKGILLSGGLDSRAILAGDHKNKIKYGFTIGDFYNKEVEISNKVADSQDMEFYFLQRNKEYYINILEKAV
EIGEGMYRFDHAHFLNYTNKLKSNVDVVFTGGLIEQLWQGSKFFYYNLQNKIKTPYLLDTNSDILEFIIYKYGVKLNNYE
VFKGFNKLEFQDNLEKSLADIFSENINVNLATDFIFYNNRNPAYLNQLCLDEYIDHRLLDDTELIDFLLTVPVEFRANGH
ILRKSLKYLSKELTRIPTSNYGMPVYCNPYFEFLVSIFYKSYHKLKLKRYNKYYTNHSWPNRGQLLRDNKKFQMSVYNTI
TKLDDELFDKKTLKNLFHDHINYKKNNYEILALLLTFGKWQEMTNN

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72114; Mature: 71983

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLIGLYYMDKYENLKSKLRHLKKDINVPNASFMECWSHENLGVVRVHHGVINSEEQPI
CCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCC
FNEDGTKCIFMIGEVFGYHNEKKNLIQKGHKFKFDNDAEYCLHLFEEYGEGSFIKLNGSF
CCCCCCEEEEEEHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCE
NLIIYDFFEKKLIIVNDRFAYKPMFFCKKDNLVAFSSRIKPLLNFSDKKYNMSAIYDFFG
EEEEEEECCCEEEEEECCCCCCCEEEEECCCEEEEHHHCHHHHCCCCCCCCHHHHHHHHH
FQKIFGDKTYYKDIYSLGNAEYLIIDHKGYITKRKYWEISHNIVHRSEDEYVKILSGKLV
HHHHHCCHHHHHHHHHCCCCEEEEEECCCCEECCCEEEHHHHHHCCCCCHHHHHHHHHHH
KAMERRTSDKNKKGILLSGGLDSRAILAGDHKNKIKYGFTIGDFYNKEVEISNKVADSQD
HHHHHHCCCCCCCCEEEECCCCCCEEEECCCCCCEEEEEEECCCCCCEEEECCCCCCCCC
MEFYFLQRNKEYYINILEKAVEIGEGMYRFDHAHFLNYTNKLKSNVDVVFTGGLIEQLWQ
CEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHC
GSKFFYYNLQNKIKTPYLLDTNSDILEFIIYKYGVKLNNYEVFKGFNKLEFQDNLEKSLA
CCEEEEEEECCCCCCCEEECCCHHHHHHHHHHHCCEECCCHHHCCCCCCCHHHHHHHHHH
DIFSENINVNLATDFIFYNNRNPAYLNQLCLDEYIDHRLLDDTELIDFLLTVPVEFRANG
HHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEECCCC
HILRKSLKYLSKELTRIPTSNYGMPVYCNPYFEFLVSIFYKSYHKLKLKRYNKYYTNHSW
HHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PNRGQLLRDNKKFQMSVYNTITKLDDELFDKKTLKNLFHDHINYKKNNYEILALLLTFGK
CCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHH
WQEMTNN
HHHHCCC
>Mature Secondary Structure 
SKLIGLYYMDKYENLKSKLRHLKKDINVPNASFMECWSHENLGVVRVHHGVINSEEQPI
CCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECCCCCCCCCCC
FNEDGTKCIFMIGEVFGYHNEKKNLIQKGHKFKFDNDAEYCLHLFEEYGEGSFIKLNGSF
CCCCCCEEEEEEHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCE
NLIIYDFFEKKLIIVNDRFAYKPMFFCKKDNLVAFSSRIKPLLNFSDKKYNMSAIYDFFG
EEEEEEECCCEEEEEECCCCCCCEEEEECCCEEEEHHHCHHHHCCCCCCCCHHHHHHHHH
FQKIFGDKTYYKDIYSLGNAEYLIIDHKGYITKRKYWEISHNIVHRSEDEYVKILSGKLV
HHHHHCCHHHHHHHHHCCCCEEEEEECCCCEECCCEEEHHHHHHCCCCCHHHHHHHHHHH
KAMERRTSDKNKKGILLSGGLDSRAILAGDHKNKIKYGFTIGDFYNKEVEISNKVADSQD
HHHHHHCCCCCCCCEEEECCCCCCEEEECCCCCCEEEEEEECCCCCCEEEECCCCCCCCC
MEFYFLQRNKEYYINILEKAVEIGEGMYRFDHAHFLNYTNKLKSNVDVVFTGGLIEQLWQ
CEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHC
GSKFFYYNLQNKIKTPYLLDTNSDILEFIIYKYGVKLNNYEVFKGFNKLEFQDNLEKSLA
CCEEEEEEECCCCCCCEEECCCHHHHHHHHHHHCCEECCCHHHCCCCCCCHHHHHHHHHH
DIFSENINVNLATDFIFYNNRNPAYLNQLCLDEYIDHRLLDDTELIDFLLTVPVEFRANG
HHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEECCCC
HILRKSLKYLSKELTRIPTSNYGMPVYCNPYFEFLVSIFYKSYHKLKLKRYNKYYTNHSW
HHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PNRGQLLRDNKKFQMSVYNTITKLDDELFDKKTLKNLFHDHINYKKNNYEILALLLTFGK
CCCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHH
WQEMTNN
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584049; 9384377; 10498721 [H]