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The map label for this gene is thiM

Identifier: 188587089

GI number: 188587089

Start: 2670736

End: 2671557

Strand: Reverse

Name: thiM

Synonym: Nther_2483

Alternate gene names: 188587089

Gene position: 2671557-2670736 (Counterclockwise)

Preceding gene: 188587090

Following gene: 188587088

Centisome position: 84.39

GC content: 40.51

Gene sequence:

>822_bases
ATGAGTACATTCAATTCTAATTTTAAGTTGGGAACTTATTTGACACAAGTTAGGCAAGAAAATCCTTTAGTCCATGCTAT
TACTAATTATGTCACAATGAATGATTGCGCTAATATTACATTGGCTGCCGGCGCTTCACCGGCTATGTGTGAGAGTAGGG
ACGAAGTATCTGATTTTGTGCCCCTGGCTAAAGCACTTTATATAAATATCGGTACAATAAATCAAGAACACAAGGATTCC
ATTTATCTGGCAGCAGAAAAAGCTTCAGAATTAGAAATACCAATTGTTCTAGACCCGGTGGGTGCTGTGGCCATAAAAAG
CAGGCTGGACTTGGTGAAAGATTTACTGACTAATTACAATGTGAGCTGCATCAAAGGTAATAATGCTGAAATTAAATGCC
TTGCTGGTCGCAAAGGTCATGGAAAAGGAATGGACTCCCTGGACTTGGGTGAGGACATTCAAATGGTCAATTCAGAGCTC
TCTGAGAAATACAACACAATGGTATTGGCAACGGGAAAAACAGATTTAATTACAAAAGGAAATATAACAGTTAAAGTTAG
CAATGGAACACCTTTATTAGGTCGGATTACGGGCTCGGGCTGTATGCTAGGAATCCTGATCAGTGCTTTTATCGGGGCCT
CAGATAATGACTGGGAAGCTGGAATAGCGGCAACTGTTTCCATGGGAGTTGTCGGAGAGATGGCTGAAGAGAGTATTTCT
AGTTCAACTGATCTCGGTAGTTTTCGAGTTAAAATCTTTGATCACATGGCGGCTTTAACAAGTAAAGAATTGCAAGAGAG
GGGGAATGTGTCTGAGTTATAA

Upstream 100 bases:

>100_bases
AAGCCATTAATAATAGTTTTACAATCGGTGAAGGTTCAAAAGTACTCTTGCATTCATCAATAGGAGATGAAGAAGTGCAA
GAAAAGGAGGAATTAAATGC

Downstream 100 bases:

>100_bases
GATATAAGTAGTGCGCAAGTAGTTAAAATCGCGTATGGCTGCATCTCGATGCGCTTCGTCGCACTTTCGTCGTATGATGA
AGCATCCTGATGGATGCAGA

Product: Hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSTFNSNFKLGTYLTQVRQENPLVHAITNYVTMNDCANITLAAGASPAMCESRDEVSDFVPLAKALYINIGTINQEHKDS
IYLAAEKASELEIPIVLDPVGAVAIKSRLDLVKDLLTNYNVSCIKGNNAEIKCLAGRKGHGKGMDSLDLGEDIQMVNSEL
SEKYNTMVLATGKTDLITKGNITVKVSNGTPLLGRITGSGCMLGILISAFIGASDNDWEAGIAATVSMGVVGEMAEESIS
SSTDLGSFRVKIFDHMAALTSKELQERGNVSEL

Sequences:

>Translated_273_residues
MSTFNSNFKLGTYLTQVRQENPLVHAITNYVTMNDCANITLAAGASPAMCESRDEVSDFVPLAKALYINIGTINQEHKDS
IYLAAEKASELEIPIVLDPVGAVAIKSRLDLVKDLLTNYNVSCIKGNNAEIKCLAGRKGHGKGMDSLDLGEDIQMVNSEL
SEKYNTMVLATGKTDLITKGNITVKVSNGTPLLGRITGSGCMLGILISAFIGASDNDWEAGIAATVSMGVVGEMAEESIS
SSTDLGSFRVKIFDHMAALTSKELQERGNVSEL
>Mature_272_residues
STFNSNFKLGTYLTQVRQENPLVHAITNYVTMNDCANITLAAGASPAMCESRDEVSDFVPLAKALYINIGTINQEHKDSI
YLAAEKASELEIPIVLDPVGAVAIKSRLDLVKDLLTNYNVSCIKGNNAEIKCLAGRKGHGKGMDSLDLGEDIQMVNSELS
EKYNTMVLATGKTDLITKGNITVKVSNGTPLLGRITGSGCMLGILISAFIGASDNDWEAGIAATVSMGVVGEMAEESISS
STDLGSFRVKIFDHMAALTSKELQERGNVSEL

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=248, Percent_Identity=33.4677419354839, Blast_Score=135, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325042, Length=298, Percent_Identity=32.2147651006711, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_NATTJ (B2A1A8)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001918634.1
- ProteinModelPortal:   B2A1A8
- SMR:   B2A1A8
- GeneID:   6316343
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_2483
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 29158; Mature: 29026

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: BINDING 49-49 BINDING 125-125 BINDING 171-171 BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFNSNFKLGTYLTQVRQENPLVHAITNYVTMNDCANITLAAGASPAMCESRDEVSDFV
CCCCCCCCCHHHHHHHHHHCCCEEEEHHHEEEECCCCCEEEECCCCCCHHCCHHHHHHHH
PLAKALYINIGTINQEHKDSIYLAAEKASELEIPIVLDPVGAVAIKSRLDLVKDLLTNYN
HHHHEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
VSCIKGNNAEIKCLAGRKGHGKGMDSLDLGEDIQMVNSELSEKYNTMVLATGKTDLITKG
EEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCEEECC
NITVKVSNGTPLLGRITGSGCMLGILISAFIGASDNDWEAGIAATVSMGVVGEMAEESIS
CEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
SSTDLGSFRVKIFDHMAALTSKELQERGNVSEL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STFNSNFKLGTYLTQVRQENPLVHAITNYVTMNDCANITLAAGASPAMCESRDEVSDFV
CCCCCCCCHHHHHHHHHHCCCEEEEHHHEEEECCCCCEEEECCCCCCHHCCHHHHHHHH
PLAKALYINIGTINQEHKDSIYLAAEKASELEIPIVLDPVGAVAIKSRLDLVKDLLTNYN
HHHHEEEEEECCCCCCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCC
VSCIKGNNAEIKCLAGRKGHGKGMDSLDLGEDIQMVNSELSEKYNTMVLATGKTDLITKG
EEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCEEECC
NITVKVSNGTPLLGRITGSGCMLGILISAFIGASDNDWEAGIAATVSMGVVGEMAEESIS
CEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
SSTDLGSFRVKIFDHMAALTSKELQERGNVSEL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA