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The map label for this gene is rpfG [H]

Identifier: 188587015

GI number: 188587015

Start: 2574195

End: 2576471

Strand: Direct

Name: rpfG [H]

Synonym: Nther_2407

Alternate gene names: 188587015

Gene position: 2574195-2576471 (Clockwise)

Preceding gene: 188587000

Following gene: 188587017

Centisome position: 81.32

GC content: 38.87

Gene sequence:

>2277_bases
TTGACCTCTGGAAAGAGCCTACAAAGTCTATTAAATATCTTTAAAAATATACCGAATTCCATCCAAGAGCTGCAGGATTT
TGCATTAGAAGAAGCACTTAAACTCACTGACAGCAAAATTGGCCATTTTTATAAATATAACGAGAACCTACAGCAACTGC
GCCTGTTGTCTTTTTCCCAAAAAGTATTTCAAGATTGCAGCTTAAACAGGCACAAAAAAATACGTCCCCTGGACGAACCG
GGTATATGTACTGAAGCTATCCGCAAAAGGAAGCCCTTAGTCATTAATGATTATCAAAAATCTCACCCGTTAAAAAAAGG
GATATCAGACTACCATATTAATTTGAACAGATATATGGGAATTCCTATTTTCAAAAACAATAACATAGTTGCCGTAGTCG
GTATGGCCAATAAGGAAGAGGATTACACGGAGAATGACCTTTTGAGGTTACAAATTTTTATGGATTATTTATGGGAAGTT
CGAGAACATAAAGAATTAGAAAAAGAATTATATCAAATAAAACAAGTTCTATCACCCAAGCATAAACCCCAAATCAATAC
TGTTAAAAACAAAAACTATACAGTCCAAAGAAGTACTGCACAAAGCATTACTTCCCAGAACAATGCTAGATACAAATTAA
GTGATATGCAATATGGAGATTTGACAAAATCCAATACCCGCCGGGAAATCTTAGAAGCTACTGGAAAGGATCTGTTACAT
GAAATCTCAATGGATTTTGTAAAATTGCTAGAGACCACGGCACTGATCTTTGAGAAAAATGGGGATTATGCCCTGGGATT
ATTTGCCTCCGATTGGTGTCGCTTTCTCAACAAAGCTTCGCGAAACCTGTGTTCCACAAATGATAATTGGGAAGCTATGC
AGAGCGGTCACTGGCTTTGTCACGAATCATGTTGGAGGCGAGGAGCTAAACATGCCATAGATACAGGCCAACCCGTTGAT
AAACTTTGTTATGGAGGTATACGTATACATGCGGTACCTATCCAGGCCCGGGGAACTGTTGTAGGTGCTATTAGCATGAG
CTACGGGGATCCACCCCAGGATTTGAACCACCTCCAGGACATAGCTGACTCTTACCAGGTAGATTTGGCCAGTTTATGGG
AAAAAACAAAATCTTATGATTCGCGCCCTTCATTTATAATCGACATAGCCCAAGAACGTTTATATACTGCTGCCAGGTTA
ATCGGATATATCATGGAAAGAAAGAAAGCTGAAAATCAACTCCATTATTTAAGCTATCATGATAAACTAACTGGATTATA
TAATAGAGATTACATCGACAGAGAATTAAATAATCTTTCCGGGGAGTCAAATTTACCACTTAGCATAATAATGACTGATG
TCAACGGCTTAAAACTTGTTAATGAAAGTTATGGAAATGAAACCGGCAATCAAATATTAACCAATATAGCAAATTTGATT
GAAGAAATCATACCAGAAGAAGGTATTGCTGCCAGGTGGGGTGGTGATGAGTTTATCATCATTCTACCAAAAACTAGCAA
AAAACAAGCAGAAAACATTTCTGCTAATCTTAAGAGAAAATGTGATAATTTACAGGTAACCACATTTCCTGTAAAAATGG
CTCTGGGAGTTGCCACAGCCACAAAGACTGCCACAAGTACAAAAACTAAAAATTCTCTTAAAGAAACTATAAAATCCGCA
GAAGATCGCATGTACAAAAATAAGCTAGGAGAAAGCGAAAGCACCAAGAGTGATATTATCGGAGCCCTACTGGCTACCCT
AAAAGAAAAAAGCGATGAAACAGAAGCTCATGCCCGCCGGCTTAGAGAGTTGGGATTAAAATTCGGAACAGCCCTGGGAT
TATCCAGGGCTGAAAAGGATAGATTATCTTTACTGGCAACCATGCACGATATCGGCAAAGTTGTAATCTCAGAAGATATT
CTGAAAAAATCCGGCCCTTTAACTGAAGATGAGTGGGAAAAAGTCAAAGAACATCCTGAAATTGGGCAGCGAATTGCTTA
TAACTCTGATAACCTAAACCACATAGCAGAGGAGATAGCCTCCCACCACGAACGATGGGATGGTAAGGGATATCCCAGGG
GTTTACAGGGCAAGGAAATACCACTACTAGCCAGAGTAATATCTATCATTGATACTTTTGATGTCATAACCCAGGGCAGA
TCATACAAAAGAGCAGTTTCCAAGCAACAAGCCATAGCTGAAATTAAAAGGTGTGCCGGAACACAGTTTGATCCTGAGCT
GGTTGAAATTTTCACTAACTTGGTTGAGGAATGTTAA

Upstream 100 bases:

>100_bases
ATTCTTATCATTATTATTACCAGATTAATAATATAAGAGCTTTCTAAACATCTAAAAATATTTTTATTAATTAATTAATT
ATATGTGGAGGGTGAGTGTA

Downstream 100 bases:

>100_bases
TGGATAGAAGGAGTATGCACACCCTGGGACTGATCCTGAATGATTTCCACAAATAGCTTCGCCAGTTGGGGATCAAATTG
CCTTCCTCCGCAGCGCAAGA

Product: diguanylate cyclase and metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MTSGKSLQSLLNIFKNIPNSIQELQDFALEEALKLTDSKIGHFYKYNENLQQLRLLSFSQKVFQDCSLNRHKKIRPLDEP
GICTEAIRKRKPLVINDYQKSHPLKKGISDYHINLNRYMGIPIFKNNNIVAVVGMANKEEDYTENDLLRLQIFMDYLWEV
REHKELEKELYQIKQVLSPKHKPQINTVKNKNYTVQRSTAQSITSQNNARYKLSDMQYGDLTKSNTRREILEATGKDLLH
EISMDFVKLLETTALIFEKNGDYALGLFASDWCRFLNKASRNLCSTNDNWEAMQSGHWLCHESCWRRGAKHAIDTGQPVD
KLCYGGIRIHAVPIQARGTVVGAISMSYGDPPQDLNHLQDIADSYQVDLASLWEKTKSYDSRPSFIIDIAQERLYTAARL
IGYIMERKKAENQLHYLSYHDKLTGLYNRDYIDRELNNLSGESNLPLSIIMTDVNGLKLVNESYGNETGNQILTNIANLI
EEIIPEEGIAARWGGDEFIIILPKTSKKQAENISANLKRKCDNLQVTTFPVKMALGVATATKTATSTKTKNSLKETIKSA
EDRMYKNKLGESESTKSDIIGALLATLKEKSDETEAHARRLRELGLKFGTALGLSRAEKDRLSLLATMHDIGKVVISEDI
LKKSGPLTEDEWEKVKEHPEIGQRIAYNSDNLNHIAEEIASHHERWDGKGYPRGLQGKEIPLLARVISIIDTFDVITQGR
SYKRAVSKQQAIAEIKRCAGTQFDPELVEIFTNLVEEC

Sequences:

>Translated_758_residues
MTSGKSLQSLLNIFKNIPNSIQELQDFALEEALKLTDSKIGHFYKYNENLQQLRLLSFSQKVFQDCSLNRHKKIRPLDEP
GICTEAIRKRKPLVINDYQKSHPLKKGISDYHINLNRYMGIPIFKNNNIVAVVGMANKEEDYTENDLLRLQIFMDYLWEV
REHKELEKELYQIKQVLSPKHKPQINTVKNKNYTVQRSTAQSITSQNNARYKLSDMQYGDLTKSNTRREILEATGKDLLH
EISMDFVKLLETTALIFEKNGDYALGLFASDWCRFLNKASRNLCSTNDNWEAMQSGHWLCHESCWRRGAKHAIDTGQPVD
KLCYGGIRIHAVPIQARGTVVGAISMSYGDPPQDLNHLQDIADSYQVDLASLWEKTKSYDSRPSFIIDIAQERLYTAARL
IGYIMERKKAENQLHYLSYHDKLTGLYNRDYIDRELNNLSGESNLPLSIIMTDVNGLKLVNESYGNETGNQILTNIANLI
EEIIPEEGIAARWGGDEFIIILPKTSKKQAENISANLKRKCDNLQVTTFPVKMALGVATATKTATSTKTKNSLKETIKSA
EDRMYKNKLGESESTKSDIIGALLATLKEKSDETEAHARRLRELGLKFGTALGLSRAEKDRLSLLATMHDIGKVVISEDI
LKKSGPLTEDEWEKVKEHPEIGQRIAYNSDNLNHIAEEIASHHERWDGKGYPRGLQGKEIPLLARVISIIDTFDVITQGR
SYKRAVSKQQAIAEIKRCAGTQFDPELVEIFTNLVEEC
>Mature_757_residues
TSGKSLQSLLNIFKNIPNSIQELQDFALEEALKLTDSKIGHFYKYNENLQQLRLLSFSQKVFQDCSLNRHKKIRPLDEPG
ICTEAIRKRKPLVINDYQKSHPLKKGISDYHINLNRYMGIPIFKNNNIVAVVGMANKEEDYTENDLLRLQIFMDYLWEVR
EHKELEKELYQIKQVLSPKHKPQINTVKNKNYTVQRSTAQSITSQNNARYKLSDMQYGDLTKSNTRREILEATGKDLLHE
ISMDFVKLLETTALIFEKNGDYALGLFASDWCRFLNKASRNLCSTNDNWEAMQSGHWLCHESCWRRGAKHAIDTGQPVDK
LCYGGIRIHAVPIQARGTVVGAISMSYGDPPQDLNHLQDIADSYQVDLASLWEKTKSYDSRPSFIIDIAQERLYTAARLI
GYIMERKKAENQLHYLSYHDKLTGLYNRDYIDRELNNLSGESNLPLSIIMTDVNGLKLVNESYGNETGNQILTNIANLIE
EIIPEEGIAARWGGDEFIIILPKTSKKQAENISANLKRKCDNLQVTTFPVKMALGVATATKTATSTKTKNSLKETIKSAE
DRMYKNKLGESESTKSDIIGALLATLKEKSDETEAHARRLRELGLKFGTALGLSRAEKDRLSLLATMHDIGKVVISEDIL
KKSGPLTEDEWEKVKEHPEIGQRIAYNSDNLNHIAEEIASHHERWDGKGYPRGLQGKEIPLLARVISIIDTFDVITQGRS
YKRAVSKQQAIAEIKRCAGTQFDPELVEIFTNLVEEC

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Integral membrane protein (Potential) [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=29.0909090909091, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=25.4335260115607, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI1787541, Length=174, Percent_Identity=27.0114942528736, Blast_Score=68, Evalue=3e-12,
Organism=Escherichia coli, GI1787262, Length=170, Percent_Identity=25.8823529411765, Blast_Score=64, Evalue=5e-11,
Organism=Escherichia coli, GI1787802, Length=153, Percent_Identity=24.8366013071895, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 86202; Mature: 86071

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSGKSLQSLLNIFKNIPNSIQELQDFALEEALKLTDSKIGHFYKYNENLQQLRLLSFSQ
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KVFQDCSLNRHKKIRPLDEPGICTEAIRKRKPLVINDYQKSHPLKKGISDYHINLNRYMG
HHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEECCHHCCCHHCCCCCEEEEEEHEEC
IPIFKNNNIVAVVGMANKEEDYTENDLLRLQIFMDYLWEVREHKELEKELYQIKQVLSPK
CEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HKPQINTVKNKNYTVQRSTAQSITSQNNARYKLSDMQYGDLTKSNTRREILEATGKDLLH
CCCCCCEECCCCEEEEHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
EISMDFVKLLETTALIFEKNGDYALGLFASDWCRFLNKASRNLCSTNDNWEAMQSGHWLC
HHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHH
HESCWRRGAKHAIDTGQPVDKLCYGGIRIHAVPIQARGTVVGAISMSYGDPPQDLNHLQD
HHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEEEEECCEEEEEEEECCCCCHHHHHHHHH
IADSYQVDLASLWEKTKSYDSRPSFIIDIAQERLYTAARLIGYIMERKKAENQLHYLSYH
HHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
DKLTGLYNRDYIDRELNNLSGESNLPLSIIMTDVNGLKLVNESYGNETGNQILTNIANLI
HHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCCCCEEHHHHCCCCHHHHHHHHHHHHH
EEIIPEEGIAARWGGDEFIIILPKTSKKQAENISANLKRKCDNLQVTTFPVKMALGVATA
HHHCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
TKTATSTKTKNSLKETIKSAEDRMYKNKLGESESTKSDIIGALLATLKEKSDETEAHARR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
LRELGLKFGTALGLSRAEKDRLSLLATMHDIGKVVISEDILKKSGPLTEDEWEKVKEHPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHH
IGQRIAYNSDNLNHIAEEIASHHERWDGKGYPRGLQGKEIPLLARVISIIDTFDVITQGR
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SYKRAVSKQQAIAEIKRCAGTQFDPELVEIFTNLVEEC
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSGKSLQSLLNIFKNIPNSIQELQDFALEEALKLTDSKIGHFYKYNENLQQLRLLSFSQ
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
KVFQDCSLNRHKKIRPLDEPGICTEAIRKRKPLVINDYQKSHPLKKGISDYHINLNRYMG
HHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEECCHHCCCHHCCCCCEEEEEEHEEC
IPIFKNNNIVAVVGMANKEEDYTENDLLRLQIFMDYLWEVREHKELEKELYQIKQVLSPK
CEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HKPQINTVKNKNYTVQRSTAQSITSQNNARYKLSDMQYGDLTKSNTRREILEATGKDLLH
CCCCCCEECCCCEEEEHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
EISMDFVKLLETTALIFEKNGDYALGLFASDWCRFLNKASRNLCSTNDNWEAMQSGHWLC
HHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHH
HESCWRRGAKHAIDTGQPVDKLCYGGIRIHAVPIQARGTVVGAISMSYGDPPQDLNHLQD
HHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEEEEECCEEEEEEEECCCCCHHHHHHHHH
IADSYQVDLASLWEKTKSYDSRPSFIIDIAQERLYTAARLIGYIMERKKAENQLHYLSYH
HHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
DKLTGLYNRDYIDRELNNLSGESNLPLSIIMTDVNGLKLVNESYGNETGNQILTNIANLI
HHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEECCCCCEEHHHHCCCCHHHHHHHHHHHHH
EEIIPEEGIAARWGGDEFIIILPKTSKKQAENISANLKRKCDNLQVTTFPVKMALGVATA
HHHCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
TKTATSTKTKNSLKETIKSAEDRMYKNKLGESESTKSDIIGALLATLKEKSDETEAHARR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
LRELGLKFGTALGLSRAEKDRLSLLATMHDIGKVVISEDILKKSGPLTEDEWEKVKEHPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHH
IGQRIAYNSDNLNHIAEEIASHHERWDGKGYPRGLQGKEIPLLARVISIIDTFDVITQGR
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SYKRAVSKQQAIAEIKRCAGTQFDPELVEIFTNLVEEC
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]