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The map label for this gene is yeiE [H]

Identifier: 188586981

GI number: 188586981

Start: 2533605

End: 2534507

Strand: Reverse

Name: yeiE [H]

Synonym: Nther_2373

Alternate gene names: 188586981

Gene position: 2534507-2533605 (Counterclockwise)

Preceding gene: 188586983

Following gene: 188586980

Centisome position: 80.07

GC content: 37.1

Gene sequence:

>903_bases
ATGGACTTCAGGCAGCTAGAAGTGTTTGTAACAATTGTGGAAGAGGGGAACTTTTCTAAGGCGGCAAAACGTCTGTACTT
GACACAGCCTACTGTAAGTGCTCATGTTGATTCTTTAGAAAGGGAATGTGGTATGAAGTTGTTTGAAAGACGTAATCGGC
AGGCTGTCTTAACAGAGGCGGGGCAAAATTTTTATCCCTATGCTGTAGATTTACTTGATATGAAGGAACGTGCCCATGAA
AGCTTCGCTAGGTTCAATCAGGAAATTGACGGTAAAATAACAATTAATGCCAGTCAAACTCCTGGGATTTATTTACTACC
TAATTTACTGGCTCCATATCAAAAAAAGTATCCCAGGTCTAGTTTTAGGATTACTATTTCGGATACCGACGGGGTTTTTG
AAAATATACTAAATTATGAAGCCGATCTGGGTTTTGTAGGTTCACCGAAAACATCGGATAAATTAGTTGCAAAACCTTTA
ATTAATGATCAACTTGTGTTAATTGCAGACTCTCCATTTAAAGAAAAACTTGACGAACAGCAAAAGAAACAAGGTGTATT
ATATATGAAAGACCTATTAGACCTTCCTTTTATAATTAGAACTGAGGGTTCTGCAACTAGAAAGGTATTTGAAAAAACCT
TAGCGGAACAGGAAAAGTCTATCAAAAATTTACAGATTGCCGGACAGGTGGATAACCTGGAAGGAGTTAAGAGCTTTGTG
CATCAGGGTGTTGGAGTCTCTTTTGTTTCCAAGTTCAGCTTAAATCGAGATACAAGCTTAAAGAGTTTTTACATACAAGA
TCTGATGCCTGAAAGGAAATTTTATCTGATTTATCATAAGGATAGAGTATTTTCCCCTACTTGTGAACGCTTTATAGCTC
ATGTACAAGACCAAAAAATTTAG

Upstream 100 bases:

>100_bases
ACCATAAATAATGAAATTATCATAAAATTGCTGTCTAAAATTAAAACTATAACCCCCTGTATATATGAGGAGGGGTTTCG
GAGAAATCGGAGGGTTGACT

Downstream 100 bases:

>100_bases
CTGAAACAGAGAGGTGTTACGGTGGGTACAACGGATTATAATCAAAATGAATTTAATAATCAAACAGGGGAACGAATATA
TTCAACAGAGTACGAAAAGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDFRQLEVFVTIVEEGNFSKAAKRLYLTQPTVSAHVDSLERECGMKLFERRNRQAVLTEAGQNFYPYAVDLLDMKERAHE
SFARFNQEIDGKITINASQTPGIYLLPNLLAPYQKKYPRSSFRITISDTDGVFENILNYEADLGFVGSPKTSDKLVAKPL
INDQLVLIADSPFKEKLDEQQKKQGVLYMKDLLDLPFIIRTEGSATRKVFEKTLAEQEKSIKNLQIAGQVDNLEGVKSFV
HQGVGVSFVSKFSLNRDTSLKSFYIQDLMPERKFYLIYHKDRVFSPTCERFIAHVQDQKI

Sequences:

>Translated_300_residues
MDFRQLEVFVTIVEEGNFSKAAKRLYLTQPTVSAHVDSLERECGMKLFERRNRQAVLTEAGQNFYPYAVDLLDMKERAHE
SFARFNQEIDGKITINASQTPGIYLLPNLLAPYQKKYPRSSFRITISDTDGVFENILNYEADLGFVGSPKTSDKLVAKPL
INDQLVLIADSPFKEKLDEQQKKQGVLYMKDLLDLPFIIRTEGSATRKVFEKTLAEQEKSIKNLQIAGQVDNLEGVKSFV
HQGVGVSFVSKFSLNRDTSLKSFYIQDLMPERKFYLIYHKDRVFSPTCERFIAHVQDQKI
>Mature_300_residues
MDFRQLEVFVTIVEEGNFSKAAKRLYLTQPTVSAHVDSLERECGMKLFERRNRQAVLTEAGQNFYPYAVDLLDMKERAHE
SFARFNQEIDGKITINASQTPGIYLLPNLLAPYQKKYPRSSFRITISDTDGVFENILNYEADLGFVGSPKTSDKLVAKPL
INDQLVLIADSPFKEKLDEQQKKQGVLYMKDLLDLPFIIRTEGSATRKVFEKTLAEQEKSIKNLQIAGQVDNLEGVKSFV
HQGVGVSFVSKFSLNRDTSLKSFYIQDLMPERKFYLIYHKDRVFSPTCERFIAHVQDQKI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=299, Percent_Identity=28.4280936454849, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788748, Length=298, Percent_Identity=26.510067114094, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI145693105, Length=294, Percent_Identity=25.5102040816327, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082132, Length=303, Percent_Identity=25.7425742574257, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790208, Length=255, Percent_Identity=27.4509803921569, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=23.6301369863014, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788887, Length=310, Percent_Identity=26.4516129032258, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI157672245, Length=301, Percent_Identity=22.2591362126246, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081904, Length=208, Percent_Identity=25, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787879, Length=299, Percent_Identity=22.0735785953177, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790399, Length=304, Percent_Identity=23.0263157894737, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786713, Length=296, Percent_Identity=26.0135135135135, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787806, Length=168, Percent_Identity=23.8095238095238, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788297, Length=254, Percent_Identity=23.2283464566929, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788296, Length=255, Percent_Identity=22.3529411764706, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787601, Length=306, Percent_Identity=22.8758169934641, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789639, Length=123, Percent_Identity=32.520325203252, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787530, Length=260, Percent_Identity=22.6923076923077, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786508, Length=149, Percent_Identity=30.8724832214765, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790011, Length=105, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789440, Length=255, Percent_Identity=23.921568627451, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=124, Percent_Identity=31.4516129032258, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34425; Mature: 34425

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFRQLEVFVTIVEEGNFSKAAKRLYLTQPTVSAHVDSLERECGMKLFERRNRQAVLTEA
CCCCEEEEEEEEECCCCHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHC
GQNFYPYAVDLLDMKERAHESFARFNQEIDGKITINASQTPGIYLLPNLLAPYQKKYPRS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEECCHHHCHHHHHCCCC
SFRITISDTDGVFENILNYEADLGFVGSPKTSDKLVAKPLINDQLVLIADSPFKEKLDEQ
CEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCHHHHHHHH
QKKQGVLYMKDLLDLPFIIRTEGSATRKVFEKTLAEQEKSIKNLQIAGQVDNLEGVKSFV
HHHCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHH
HQGVGVSFVSKFSLNRDTSLKSFYIQDLMPERKFYLIYHKDRVFSPTCERFIAHVQDQKI
HCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDFRQLEVFVTIVEEGNFSKAAKRLYLTQPTVSAHVDSLERECGMKLFERRNRQAVLTEA
CCCCEEEEEEEEECCCCHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHC
GQNFYPYAVDLLDMKERAHESFARFNQEIDGKITINASQTPGIYLLPNLLAPYQKKYPRS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEECCHHHCHHHHHCCCC
SFRITISDTDGVFENILNYEADLGFVGSPKTSDKLVAKPLINDQLVLIADSPFKEKLDEQ
CEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCEEEEECCCHHHHHHHH
QKKQGVLYMKDLLDLPFIIRTEGSATRKVFEKTLAEQEKSIKNLQIAGQVDNLEGVKSFV
HHHCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHH
HQGVGVSFVSKFSLNRDTSLKSFYIQDLMPERKFYLIYHKDRVFSPTCERFIAHVQDQKI
HCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]