Click here to switch to the map view.

The map label for this gene is buk1 [H]

Identifier: 188586956

GI number: 188586956

Start: 2498324

End: 2499382

Strand: Reverse

Name: buk1 [H]

Synonym: Nther_2348

Alternate gene names: 188586956

Gene position: 2499382-2498324 (Counterclockwise)

Preceding gene: 188586957

Following gene: 188586955

Centisome position: 78.96

GC content: 41.83

Gene sequence:

>1059_bases
TTGAAAATACTAGTAATCAATCCGGGATCAACTTCTACGAAGATTGCCCTTTATGAAAAGGAGCAATCCAAACTGGAAAA
GACTGTTCGTCATGATAGTGAGGAGCTAGAAAAATTTTCAGAATTGGCAGATCAGTTCGAATACCGTTTAGGTCATGTGA
AAGAAGCCGTCCAAGAAGGAGGTTGGGAGCTTCATGAGCTAGATGCAGTAGTTGCCAGGGGAGGACTGTTAAAACCAGTC
CCTGGAGGCACTTTTGAAATTAATGAAAAAATGGTTGACGATCTTAAAAAAGCCCGTTACGGTGAACATGCTTCCAATTT
GGGTGCCATTATGGCTGACAGTTTAAGGGATGAACTCAATATACCTGCCTATATAGTAGATCCGGTAGCTGTGGATGAAT
TTGAAGAAGTAGCCCGCCTTTCGGGTTTGAAGGACCTGGAAAGGATAAGCCAATCCCATGCTCTTAATATGAAAGCTGTA
GCTCGTGAAGTTGCAGAAAACATGGGTAAATCTTATTTTGATGTCAATTTGATTGTCTGTCACTTGGGCAGTGGTATTTC
AGTAGCTCCTCATAAAAATGGAAAAATGATTGATGTAAACAATGCCAATAATGAAGGCCCCTTCGCTACAGAAAGAGCTG
GTACTCTTCCTGCCTATAATTTAGTAAGACTCTGTTACTCAGGGCGCTACAGTGAAGAGGAAATGGTTAAAAAAGTGATC
AAAGAAGGCGGAATTTACTCTTATATGGGGACAAAGGATGTCTCTAAAGTGGAAGAACAGGCAAAAAGTGGTGACGAGCG
AGCCAAGCTGGTAATAGATGCCATGATTTATCAGATAAGTAAGGAAATCGGGGCTATGGCAACTGTTCTGGAAGGTCAGG
TGGATGCTATAGTTATTACTGGTGGTATGGCAAACTCCAAGTACATTATTGGCAAAATTCAGAAAAGAGTGGACTTTATA
GCTCCTGTGAAAATTGTTCCCGGTGAACGGGAATTAGAGGCTTTGGCCCTGGGGGCTTATCGAGTCCTGTCAGGAAAAGA
ACAGCCAAATATTTACTAA

Upstream 100 bases:

>100_bases
AACTGGTTGAGGAGCGAATAGCAACAGTTAAAGGATTGCAATCTAATTACATGGGTTAATATAATTAGCGGTAAAATTTG
GCTTGAAGGAGGGAGTTAAT

Downstream 100 bases:

>100_bases
TAAAAGTTAAATAAAAGTATATCCTGGAATGTAAAGGGGGAACACTATGTACAAGGATTTTTCAGAATTGCTGGCTAGTG
CCAGAAAAATGAATCCTGTT

Product: butyrate kinase

Products: NA

Alternate protein names: BK 1; Branched-chain carboxylic acid kinase 1 [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKILVINPGSTSTKIALYEKEQSKLEKTVRHDSEELEKFSELADQFEYRLGHVKEAVQEGGWELHELDAVVARGGLLKPV
PGGTFEINEKMVDDLKKARYGEHASNLGAIMADSLRDELNIPAYIVDPVAVDEFEEVARLSGLKDLERISQSHALNMKAV
AREVAENMGKSYFDVNLIVCHLGSGISVAPHKNGKMIDVNNANNEGPFATERAGTLPAYNLVRLCYSGRYSEEEMVKKVI
KEGGIYSYMGTKDVSKVEEQAKSGDERAKLVIDAMIYQISKEIGAMATVLEGQVDAIVITGGMANSKYIIGKIQKRVDFI
APVKIVPGERELEALALGAYRVLSGKEQPNIY

Sequences:

>Translated_352_residues
MKILVINPGSTSTKIALYEKEQSKLEKTVRHDSEELEKFSELADQFEYRLGHVKEAVQEGGWELHELDAVVARGGLLKPV
PGGTFEINEKMVDDLKKARYGEHASNLGAIMADSLRDELNIPAYIVDPVAVDEFEEVARLSGLKDLERISQSHALNMKAV
AREVAENMGKSYFDVNLIVCHLGSGISVAPHKNGKMIDVNNANNEGPFATERAGTLPAYNLVRLCYSGRYSEEEMVKKVI
KEGGIYSYMGTKDVSKVEEQAKSGDERAKLVIDAMIYQISKEIGAMATVLEGQVDAIVITGGMANSKYIIGKIQKRVDFI
APVKIVPGERELEALALGAYRVLSGKEQPNIY
>Mature_352_residues
MKILVINPGSTSTKIALYEKEQSKLEKTVRHDSEELEKFSELADQFEYRLGHVKEAVQEGGWELHELDAVVARGGLLKPV
PGGTFEINEKMVDDLKKARYGEHASNLGAIMADSLRDELNIPAYIVDPVAVDEFEEVARLSGLKDLERISQSHALNMKAV
AREVAENMGKSYFDVNLIVCHLGSGISVAPHKNGKMIDVNNANNEGPFATERAGTLPAYNLVRLCYSGRYSEEEMVKKVI
KEGGIYSYMGTKDVSKVEEQAKSGDERAKLVIDAMIYQISKEIGAMATVLEGQVDAIVITGGMANSKYIIGKIQKRVDFI
APVKIVPGERELEALALGAYRVLSGKEQPNIY

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=162, Percent_Identity=29.0123456790123, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 38764; Mature: 38764

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS01075 ACETATE_KINASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVINPGSTSTKIALYEKEQSKLEKTVRHDSEELEKFSELADQFEYRLGHVKEAVQEG
CEEEEECCCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GWELHELDAVVARGGLLKPVPGGTFEINEKMVDDLKKARYGEHASNLGAIMADSLRDELN
CCCHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
IPAYIVDPVAVDEFEEVARLSGLKDLERISQSHALNMKAVAREVAENMGKSYFDVNLIVC
CCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEE
HLGSGISVAPHKNGKMIDVNNANNEGPFATERAGTLPAYNLVRLCYSGRYSEEEMVKKVI
ECCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
KEGGIYSYMGTKDVSKVEEQAKSGDERAKLVIDAMIYQISKEIGAMATVLEGQVDAIVIT
HHCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
GGMANSKYIIGKIQKRVDFIAPVKIVPGERELEALALGAYRVLSGKEQPNIY
CCCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKILVINPGSTSTKIALYEKEQSKLEKTVRHDSEELEKFSELADQFEYRLGHVKEAVQEG
CEEEEECCCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GWELHELDAVVARGGLLKPVPGGTFEINEKMVDDLKKARYGEHASNLGAIMADSLRDELN
CCCHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
IPAYIVDPVAVDEFEEVARLSGLKDLERISQSHALNMKAVAREVAENMGKSYFDVNLIVC
CCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEE
HLGSGISVAPHKNGKMIDVNNANNEGPFATERAGTLPAYNLVRLCYSGRYSEEEMVKKVI
ECCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
KEGGIYSYMGTKDVSKVEEQAKSGDERAKLVIDAMIYQISKEIGAMATVLEGQVDAIVIT
HHCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
GGMANSKYIIGKIQKRVDFIAPVKIVPGERELEALALGAYRVLSGKEQPNIY
CCCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]