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The map label for this gene is glnA [H]

Identifier: 188586879

GI number: 188586879

Start: 2415355

End: 2416677

Strand: Direct

Name: glnA [H]

Synonym: Nther_2270

Alternate gene names: 188586879

Gene position: 2415355-2416677 (Clockwise)

Preceding gene: 188586875

Following gene: 188586880

Centisome position: 76.3

GC content: 40.67

Gene sequence:

>1323_bases
ATGAAACCCGAAGATATTATAGCGGCGGTAAAAGAACATAATGTAGAGTTTATTCGTCTTCAGTTCACAGATATCACAGG
TGTACTGAAAAACGTGGCCATAACTGTCAACCAGCTCGATAAAGCCCTGGACGGCGAATTGATGTTTGATGGTTCTTCTA
TTGAAGGTTTTGTCAGGATTGAAGAATCGGATATGTATTTAAAACCCGATCCTGATACTTTTATGATCTTCCCTTGGACA
AACAATGGAAGTAAAATTGCTCGAATTATCTGCGATGTTTACGATGCCAATCAAGAGCCCTTCATTGGTTGTCCCAGATG
TACTTTAAGACGTGCCATCCAGGAAGCGGACGAAATGGGATATTCCATGAAAGTAGGACCGGAAAATGAATTTTTCTTAT
TCCACACTGGTTCCGACGGTGAACCTACCCTAAAAACCCACGATAAAGCGGGATATTTCGATCTAGCTCCCGTTGATAAA
GGCGAAGAAGCCAGACGAGATATGGCTATAACTCTTCAGCAAATGGGTTTTGAAATAGAAGCTACTCACCACGAAGTTGC
CCCAGGCCAGCACGAAATCGATTTCAAATATGAAGATGCCCTAAAAACAGCCGATATGATCGCCACATTCCGCTATGTTG
TCAGAACTATCGCCAAGCAACACGACCTTCATGCCACTTTCATGCCAAAACCCATCCAAGGTATCAACGGTTCCGGAATG
CATGCAAATATTAGCTTATTTAAAAACGGGAAAAACGCTTTTTATGATCCCGAGGATCCCAATGGACTTAGCCAAGAATG
CTATTATTTTATCGGAGGTTTATTACAGCATGCCAAAGCTATGTCAGCCATAACCAATCCCACCGTCAATTCATTTAAAC
GTCTTGTTCCGGGATATGAAGCTCCTGTATATATTGCCTGGAGTGAGAAAAATAGAAGTCCCCTGATACGAATTCCAGCA
AAAAGAGAAACTTCTACCAGGGTAGAGCTTCGTAATCCCGATACTGCTTGTAATCCATATTTAGCTACAGCAGTTATGTT
AAAAGCAGGCTTACATGGTATTAAGCACAAAATTGAGCCTCCTGCATCAATTGACAAAAATATTTATAATATGACTCCAG
AAGAACGAAAAGAACTGGGAATTGATAGACTACCTGAGAATTTATTGGAGGCATTGACTTACTTGCAAGCAGATGGTGTT
ATTCAGGAAGGTTTAGGCGATCATATCTACAAAAGATTCATCGATGCTAAAAAATACGAATGGCAGAGTTATACCGAAGC
TGTTCATCCTTGGGAAAGAGAGCAATACTTGACTAAATTTTAA

Upstream 100 bases:

>100_bases
CATCGCTTTTCTGACATAATTGACACTCATACTATAAAGTGCTAAAATTTTAAGATATTGATAATCAGTCTCAGAGAAAA
TATAAAGGGAGTGACAATTT

Downstream 100 bases:

>100_bases
ATTCCAAATATCTGTTAAAAAGCTGGCTAATTAGCCAGCTTTTTTATTTGCAAATTTTTAAACTTAATTATGAATTAAAG
TTTAATGTTGATAACTTTAA

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKPEDIIAAVKEHNVEFIRLQFTDITGVLKNVAITVNQLDKALDGELMFDGSSIEGFVRIEESDMYLKPDPDTFMIFPWT
NNGSKIARIICDVYDANQEPFIGCPRCTLRRAIQEADEMGYSMKVGPENEFFLFHTGSDGEPTLKTHDKAGYFDLAPVDK
GEEARRDMAITLQQMGFEIEATHHEVAPGQHEIDFKYEDALKTADMIATFRYVVRTIAKQHDLHATFMPKPIQGINGSGM
HANISLFKNGKNAFYDPEDPNGLSQECYYFIGGLLQHAKAMSAITNPTVNSFKRLVPGYEAPVYIAWSEKNRSPLIRIPA
KRETSTRVELRNPDTACNPYLATAVMLKAGLHGIKHKIEPPASIDKNIYNMTPEERKELGIDRLPENLLEALTYLQADGV
IQEGLGDHIYKRFIDAKKYEWQSYTEAVHPWEREQYLTKF

Sequences:

>Translated_440_residues
MKPEDIIAAVKEHNVEFIRLQFTDITGVLKNVAITVNQLDKALDGELMFDGSSIEGFVRIEESDMYLKPDPDTFMIFPWT
NNGSKIARIICDVYDANQEPFIGCPRCTLRRAIQEADEMGYSMKVGPENEFFLFHTGSDGEPTLKTHDKAGYFDLAPVDK
GEEARRDMAITLQQMGFEIEATHHEVAPGQHEIDFKYEDALKTADMIATFRYVVRTIAKQHDLHATFMPKPIQGINGSGM
HANISLFKNGKNAFYDPEDPNGLSQECYYFIGGLLQHAKAMSAITNPTVNSFKRLVPGYEAPVYIAWSEKNRSPLIRIPA
KRETSTRVELRNPDTACNPYLATAVMLKAGLHGIKHKIEPPASIDKNIYNMTPEERKELGIDRLPENLLEALTYLQADGV
IQEGLGDHIYKRFIDAKKYEWQSYTEAVHPWEREQYLTKF
>Mature_440_residues
MKPEDIIAAVKEHNVEFIRLQFTDITGVLKNVAITVNQLDKALDGELMFDGSSIEGFVRIEESDMYLKPDPDTFMIFPWT
NNGSKIARIICDVYDANQEPFIGCPRCTLRRAIQEADEMGYSMKVGPENEFFLFHTGSDGEPTLKTHDKAGYFDLAPVDK
GEEARRDMAITLQQMGFEIEATHHEVAPGQHEIDFKYEDALKTADMIATFRYVVRTIAKQHDLHATFMPKPIQGINGSGM
HANISLFKNGKNAFYDPEDPNGLSQECYYFIGGLLQHAKAMSAITNPTVNSFKRLVPGYEAPVYIAWSEKNRSPLIRIPA
KRETSTRVELRNPDTACNPYLATAVMLKAGLHGIKHKIEPPASIDKNIYNMTPEERKELGIDRLPENLLEALTYLQADGV
IQEGLGDHIYKRFIDAKKYEWQSYTEAVHPWEREQYLTKF

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=455, Percent_Identity=25.0549450549451, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1790301, Length=477, Percent_Identity=39.832285115304, Blast_Score=301, Evalue=7e-83,
Organism=Escherichia coli, GI87081870, Length=376, Percent_Identity=28.1914893617021, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17540008, Length=319, Percent_Identity=26.6457680250784, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49949; Mature: 49949

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEDIIAAVKEHNVEFIRLQFTDITGVLKNVAITVNQLDKALDGELMFDGSSIEGFVRI
CCHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEE
EESDMYLKPDPDTFMIFPWTNNGSKIARIICDVYDANQEPFIGCPRCTLRRAIQEADEMG
ECCCEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
YSMKVGPENEFFLFHTGSDGEPTLKTHDKAGYFDLAPVDKGEEARRDMAITLQQMGFEIE
CEEEECCCCCEEEEECCCCCCCCEEECCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEE
ATHHEVAPGQHEIDFKYEDALKTADMIATFRYVVRTIAKQHDLHATFMPKPIQGINGSGM
ECCCCCCCCCCEECEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCC
HANISLFKNGKNAFYDPEDPNGLSQECYYFIGGLLQHAKAMSAITNPTVNSFKRLVPGYE
EEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
APVYIAWSEKNRSPLIRIPAKRETSTRVELRNPDTACNPYLATAVMLKAGLHGIKHKIEP
CCEEEEECCCCCCCEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PASIDKNIYNMTPEERKELGIDRLPENLLEALTYLQADGVIQEGLGDHIYKRFIDAKKYE
CCHHCCHHHCCCHHHHHHCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
WQSYTEAVHPWEREQYLTKF
HHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure
MKPEDIIAAVKEHNVEFIRLQFTDITGVLKNVAITVNQLDKALDGELMFDGSSIEGFVRI
CCHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEE
EESDMYLKPDPDTFMIFPWTNNGSKIARIICDVYDANQEPFIGCPRCTLRRAIQEADEMG
ECCCEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
YSMKVGPENEFFLFHTGSDGEPTLKTHDKAGYFDLAPVDKGEEARRDMAITLQQMGFEIE
CEEEECCCCCEEEEECCCCCCCCEEECCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEE
ATHHEVAPGQHEIDFKYEDALKTADMIATFRYVVRTIAKQHDLHATFMPKPIQGINGSGM
ECCCCCCCCCCEECEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCC
HANISLFKNGKNAFYDPEDPNGLSQECYYFIGGLLQHAKAMSAITNPTVNSFKRLVPGYE
EEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCC
APVYIAWSEKNRSPLIRIPAKRETSTRVELRNPDTACNPYLATAVMLKAGLHGIKHKIEP
CCEEEEECCCCCCCEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PASIDKNIYNMTPEERKELGIDRLPENLLEALTYLQADGVIQEGLGDHIYKRFIDAKKYE
CCHHCCHHHCCCHHHHHHCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
WQSYTEAVHPWEREQYLTKF
HHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2891680; 1981087 [H]