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The map label for this gene is mdeA [H]

Identifier: 188586854

GI number: 188586854

Start: 2381959

End: 2383131

Strand: Reverse

Name: mdeA [H]

Synonym: Nther_2245

Alternate gene names: 188586854

Gene position: 2383131-2381959 (Counterclockwise)

Preceding gene: 188586855

Following gene: 188586853

Centisome position: 75.28

GC content: 39.05

Gene sequence:

>1173_bases
ATGTCGAGTCAAAATTTTGAGACAAGGATTTTACATGCCGATGAAGGTCACAAGAATGATGCAGGTGCACTTAGGGTACC
AATTTATCAAAGCTCAACCTTTGTATTTGACAGTGTTGAGCAGGGTGCGTCCAGGTTTACAGGTGAAGAAGATGGCTACA
TTTACACCAGATTAGGTAATCCCACTCAATCAAGCTTAGAAAAACTTATGGCAAATTTAGAGGGAGGAGAAGACGCTCTC
GCCGTGAGTTCAGGCATGGCAGCTATCTCTTCTGCTATCATGAGTTTACTCAGCTGTGGGGATCATATGATCGTTTCAAA
TCCTCTGTATGGATGTACTCATTCTTTTTGTGTGGATATATTGCCTGAATGGGGTATTGAAGTGACTTTTGTCGATATGA
CAGATCCAGACAATATTTACAAAGCGATAAAAAGTAATACCAAAGTTATTTACACCGAGACACCGGCTAATCCTACCTTA
TATCTAATAGATTTAGAACGAGTTGCGAAAATTGCCAAGGAGAAAAATCTTACCACAATAACTGACAATACGTTCATGAG
CCCATATCTACAAAGACCACTTGAGTTGGGCATAGATGTGGTTGTCCATAGTGCTACTAAATATCTCTGTGGGCATGGTG
ATACTGTAGCTGGAATGATAGTAGGACCAAAAAAGATAATTGACAACATTAATGGGACTACAATTAAAAACTTCGGTGGA
ATTATAGGCCCCTTTGATGCTTTCTTGATTTTGAGAGGAGTTAAGACACTAACACTTCGGATGGACAAGATTTGCGAAAA
TGCCAAAAAAATAGCTGAATTTCTCGATAATCACCCTGCCATAGAAAAAGTGTATTATCCAGGACTGGAAAGTCACCCTC
AACACGAACTAGCTAAGCGACAGATGAGAAACTTTGGTGGAATCGTGACCTTCGAACTTGCTGATGGCTATGAAGCCGGG
AAAAAACTGCTTAACAATGTTGAAGTTTGTACTCTAGCGACTAGTTTAGGTGATGTAGATACATTGATTCAGCATCCAGC
TTCCATGACTCATTATGTAGTTCCTAAAGAAGAACGTGAGAAAAATGGTATTACTGATGGATTAGTAAGACTTTCTGTAG
GAATAGAAAATGTCAACGATATTATTGAGGATTTAAAACAAGGATTGAACTAA

Upstream 100 bases:

>100_bases
CAGCAATTATTATTAGAAAAACCCGACAAGGCAGTTTGATGGTCAATGGTAAGTATTAATTTAACATAGATAACAAAAAT
TTATGGAGGTGTTTTCTAAG

Downstream 100 bases:

>100_bases
GATATCTACAGGAGTGATGAAAATGAAATCTGAACAGATAAATCATCTAGGAGTCATTGCGGGAACACCGGTTGATACGG
AAATGGGGGAGATGCTGTTA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSSQNFETRILHADEGHKNDAGALRVPIYQSSTFVFDSVEQGASRFTGEEDGYIYTRLGNPTQSSLEKLMANLEGGEDAL
AVSSGMAAISSAIMSLLSCGDHMIVSNPLYGCTHSFCVDILPEWGIEVTFVDMTDPDNIYKAIKSNTKVIYTETPANPTL
YLIDLERVAKIAKEKNLTTITDNTFMSPYLQRPLELGIDVVVHSATKYLCGHGDTVAGMIVGPKKIIDNINGTTIKNFGG
IIGPFDAFLILRGVKTLTLRMDKICENAKKIAEFLDNHPAIEKVYYPGLESHPQHELAKRQMRNFGGIVTFELADGYEAG
KKLLNNVEVCTLATSLGDVDTLIQHPASMTHYVVPKEEREKNGITDGLVRLSVGIENVNDIIEDLKQGLN

Sequences:

>Translated_390_residues
MSSQNFETRILHADEGHKNDAGALRVPIYQSSTFVFDSVEQGASRFTGEEDGYIYTRLGNPTQSSLEKLMANLEGGEDAL
AVSSGMAAISSAIMSLLSCGDHMIVSNPLYGCTHSFCVDILPEWGIEVTFVDMTDPDNIYKAIKSNTKVIYTETPANPTL
YLIDLERVAKIAKEKNLTTITDNTFMSPYLQRPLELGIDVVVHSATKYLCGHGDTVAGMIVGPKKIIDNINGTTIKNFGG
IIGPFDAFLILRGVKTLTLRMDKICENAKKIAEFLDNHPAIEKVYYPGLESHPQHELAKRQMRNFGGIVTFELADGYEAG
KKLLNNVEVCTLATSLGDVDTLIQHPASMTHYVVPKEEREKNGITDGLVRLSVGIENVNDIIEDLKQGLN
>Mature_389_residues
SSQNFETRILHADEGHKNDAGALRVPIYQSSTFVFDSVEQGASRFTGEEDGYIYTRLGNPTQSSLEKLMANLEGGEDALA
VSSGMAAISSAIMSLLSCGDHMIVSNPLYGCTHSFCVDILPEWGIEVTFVDMTDPDNIYKAIKSNTKVIYTETPANPTLY
LIDLERVAKIAKEKNLTTITDNTFMSPYLQRPLELGIDVVVHSATKYLCGHGDTVAGMIVGPKKIIDNINGTTIKNFGGI
IGPFDAFLILRGVKTLTLRMDKICENAKKIAEFLDNHPAIEKVYYPGLESHPQHELAKRQMRNFGGIVTFELADGYEAGK
KLLNNVEVCTLATSLGDVDTLIQHPASMTHYVVPKEEREKNGITDGLVRLSVGIENVNDIIEDLKQGLN

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=373, Percent_Identity=41.0187667560322, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI299473759, Length=373, Percent_Identity=37.5335120643432, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI25453487, Length=372, Percent_Identity=35.4838709677419, Blast_Score=211, Evalue=6e-55,
Organism=Escherichia coli, GI1790375, Length=371, Percent_Identity=36.1185983827493, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1789383, Length=399, Percent_Identity=29.3233082706767, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71980708, Length=395, Percent_Identity=41.0126582278481, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17538055, Length=376, Percent_Identity=41.7553191489362, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17560056, Length=389, Percent_Identity=41.9023136246787, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI193202265, Length=114, Percent_Identity=37.719298245614, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319307, Length=395, Percent_Identity=38.7341772151899, Blast_Score=266, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=33.4862385321101, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321254, Length=399, Percent_Identity=31.5789473684211, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=319, Percent_Identity=30.4075235109718, Blast_Score=162, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6321904, Length=398, Percent_Identity=27.3869346733668, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19922554, Length=389, Percent_Identity=39.3316195372751, Blast_Score=280, Evalue=9e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 42806; Mature: 42675

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQNFETRILHADEGHKNDAGALRVPIYQSSTFVFDSVEQGASRFTGEEDGYIYTRLGN
CCCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEEEHHHHHHHHHCCCCCCCEEEEECCC
PTQSSLEKLMANLEGGEDALAVSSGMAAISSAIMSLLSCGDHMIVSNPLYGCTHSFCVDI
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHH
LPEWGIEVTFVDMTDPDNIYKAIKSNTKVIYTETPANPTLYLIDLERVAKIAKEKNLTTI
HHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCEEEEEEHHHHHHHHHHCCCEEE
TDNTFMSPYLQRPLELGIDVVVHSATKYLCGHGDTVAGMIVGPKKIIDNINGTTIKNFGG
ECCCHHCHHHHCHHHCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHHCCCCCCEEHHCCC
IIGPFDAFLILRGVKTLTLRMDKICENAKKIAEFLDNHPAIEKVYYPGLESHPQHELAKR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCCCCCHHHHHHH
QMRNFGGIVTFELADGYEAGKKLLNNVEVCTLATSLGDVDTLIQHPASMTHYVVPKEERE
HHHHCCCEEEEEECCCHHHHHHHHCCCCEEEEEHHHCHHHHHHHCCCCCEEEECCCHHHH
KNGITDGLVRLSVGIENVNDIIEDLKQGLN
HCCCHHHHEEEEECHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSQNFETRILHADEGHKNDAGALRVPIYQSSTFVFDSVEQGASRFTGEEDGYIYTRLGN
CCCCCEEEEEECCCCCCCCCCEEEEEEECCCEEEEHHHHHHHHHCCCCCCCEEEEECCC
PTQSSLEKLMANLEGGEDALAVSSGMAAISSAIMSLLSCGDHMIVSNPLYGCTHSFCVDI
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHH
LPEWGIEVTFVDMTDPDNIYKAIKSNTKVIYTETPANPTLYLIDLERVAKIAKEKNLTTI
HHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCEEEEEEHHHHHHHHHHCCCEEE
TDNTFMSPYLQRPLELGIDVVVHSATKYLCGHGDTVAGMIVGPKKIIDNINGTTIKNFGG
ECCCHHCHHHHCHHHCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHHCCCCCCEEHHCCC
IIGPFDAFLILRGVKTLTLRMDKICENAKKIAEFLDNHPAIEKVYYPGLESHPQHELAKR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEECCCCCCCCHHHHHHH
QMRNFGGIVTFELADGYEAGKKLLNNVEVCTLATSLGDVDTLIQHPASMTHYVVPKEERE
HHHHCCCEEEEEECCCHHHHHHHHCCCCEEEEEHHHCHHHHHHHCCCCCEEEECCCHHHH
KNGITDGLVRLSVGIENVNDIIEDLKQGLN
HCCCHHHHEEEEECHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]