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The map label for this gene is pbpF [H]

Identifier: 188586679

GI number: 188586679

Start: 2188011

End: 2190542

Strand: Direct

Name: pbpF [H]

Synonym: Nther_2069

Alternate gene names: 188586679

Gene position: 2188011-2190542 (Clockwise)

Preceding gene: 188586662

Following gene: 188586683

Centisome position: 69.12

GC content: 38.59

Gene sequence:

>2532_bases
GTGCTTATAATTTTATTTTTAGGTGGCGGCACGGCCTTTGGTATGGTTGTAGCAGCTCTTCAAACAGTACCAGAATTTGA
ACCAGGTCAACTAGAAGCTACAGTACCATCAAAAATAAAGGACCCCGACGGAAATGAAATTGTCCGTCTCGACAGAGAAC
ACCAGCGAGAAGAAGTATCTTTAGACCAAGTACCAGATCATGTAATTGAAGCTTTCATAGCCATCGAAGATGCCAGGTTT
GAGGAACACTTTGGTTTCGATGTTCGAGGCATCAGTCGAGCGGCAGTCAATAATTTTCAAGAAACTGGTAATCCATTTAA
AGGTAGCCAGGGTGGAAGTACTATAACACAGCAGTTGGTTAAAAATACTTTTTTATCACCAAAACGGTTACTTGAAAGGA
AAATACATGAGGCTTGGTTAGCTCTACACGTAGAGCGTACTTATACTAAAGAAGAGATAATGGAGTATTATTTAAACTAT
GCCACATACTTCCACCACAATATCTATGGGATACAAGCAGCTAGTAATTTTTATTTTGACAAAGATGTCAGCGATTTAGA
TGTTGAAGAAGGCGCGCTTCTAGCAGGAATAATTCGTCATCCAAGTCGACTATCTCCACATAACAATCCGGATCATTCTA
AACAAAGGCAGGAAACTGTACTTCATTCTATGAAAAGTCAAGACTTTATCTCAGAATCAGAATTCAACAAGGCTTTAAAT
AAAGAACTGGATGAAATATTAGCATCGAAACCTGAAAAACAATATCCCTACCCTCATTTTATTGATTATGTCATCAACGA
AGAAGCTAAACCTATCTTAGAGGATAAAATTGGTTCTGTAGAAAGTTTACCAGATAATGTACAAAATGCAGAAGATCTAC
TATATCATCACGGTTTAACAATACACACCACTATTGATAGAGAATTACAGAGTTATTCTGAAGATATAATGGATAATCCA
GAGAATTATCCAGAAACTTGGGAAGATGAAATGGGAGTATTGCAACCCCAAGGCGCTTTAGTAGTTTCAGATCCCAATAC
AGGTGAGGTCAGAGCTATGGTTGGCGGTCGAGATTATGGTACACACAATATGGTGAATAGGGTTACAAGTACAAGATCAC
CAGGTTCAGCAATGAAACCGATTAATGTATATGCACCTGCTATAGAAGAAGGAAAGTTAACTCCTGGTACTGTAATTGAT
GATGCTCCTTCCAGCTGGGAAGATGGAGGGGACTCTTACGAACCTGAAAACTTTACCAATACGTTTAGAGGATTGACAAC
AGTTCGAGAAGCTTTGGTAGATTCATTAAATATCCCTGCTGTTAGGGTCTACGATGACAGATTAGGCACAGAAATTGGCG
CCGAATACGCCGAAAAATTTGGTGTAACCACCTTTACTGAAGGAGATAGACATAACTTAGCAGCTTCTATTGGTGGATTA
ACAAAAGGGGTTAAACCCTTAGAGCTAACTGAAGCTTACGGAACTCTAGCTAATAACGGAATCAGAGTAGAAAACCATAC
AATTACAAAAATAGAAGATAGGTATGGCAATACCATCTATGAAAGAGAACCAGATTATGATGTAGTAATTTCTGAAGAAA
CATCCTGGCTGATTACAGATATTTTGCAAGATGTGGTTACAGAAGGAACTGCTGCTTCACTCAATTTAAACTTTCCGGCA
GCTGCTAAAACAGGAACATCTAATAACTTCAGAGATGCATGGTTAGTAGGTTACACACCTAATTTAGTTGCGAGTTTCTG
GCTTGGCTATGATTATGATGATATTCAAGTTCAAAACAGGACACATTTTACTACTAATATAATTGAAAATGTCATGACAC
ATGCCCTTAAGGATGAAAATCCTCCAGAATTTCAGAAACCAGATGGTATTGAAGGACCAATTGAAATAAGCGAGAAATCT
GGCATGCGAGCTTCTGATAAAACACCTTCAGAATATACGACTTACGAGTTTTTCCCTGAAGATCAAATTCCGACTGGTGA
ATGTGATATATTTGTAGAAAAAAAAGTTTGCACTGCCCATAAATACAGTGATAATGAAGAAGGGGATAACTCAGCAAGCA
ATCAAGAACCAAAATTAGCCAGTGATAATTGTCCACCTGAGGCAACGGAGACTCGGGTTTTCCTTGATAGAGATGACCCT
GGGTTCACTGATGATGGTAGAACAACATATGATGCAAGTCTTATGCCTCCAGAAGAAGAATGTACTCTACATGAAAAAGG
GGACTTTCCTGAAGATGAGCTTGAAGACGAAGACAATGGTCTCGATTTCTTCGATGACGACGATGATCATAAAGGAGAAG
ATTTAGAAGATATCCTTGAGGACGAAGACCAAGAAGATGAAGAAATTGATGATGAGGTTGAAGAAAGCCATGGAGATGAA
AGTAGCCAAGATCGAGATAATGGAACAGACCAAGATGAAGATGCATCTGAAGAAAAAGAAGAAGAGAACGAGGAAGAACT
AGAAGACACCAACAACAATGATAATGCCAGTGATGATGAAAACACTAGTTAA

Upstream 100 bases:

>100_bases
ATCAGTAACTAACCAGCATAACAATAAAAATAAAGGTAAACAAAAAAAACGTAAACTAAAATTCAAACCGTCTGCGCTAT
TAAAGCTAATCTTTGTATCT

Downstream 100 bases:

>100_bases
GATAACTTAGTTAAAAAAGAAAATAAAAAATTAACCTAATAAGAAGTGAGCTCTCCCTCAATTTGGAGAGCTCACTTTTT
ATATTATCATGTTTACGTTA

Product: penicillin-binding protein, 1A family

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 843; Mature: 843

Protein sequence:

>843_residues
MLIILFLGGGTAFGMVVAALQTVPEFEPGQLEATVPSKIKDPDGNEIVRLDREHQREEVSLDQVPDHVIEAFIAIEDARF
EEHFGFDVRGISRAAVNNFQETGNPFKGSQGGSTITQQLVKNTFLSPKRLLERKIHEAWLALHVERTYTKEEIMEYYLNY
ATYFHHNIYGIQAASNFYFDKDVSDLDVEEGALLAGIIRHPSRLSPHNNPDHSKQRQETVLHSMKSQDFISESEFNKALN
KELDEILASKPEKQYPYPHFIDYVINEEAKPILEDKIGSVESLPDNVQNAEDLLYHHGLTIHTTIDRELQSYSEDIMDNP
ENYPETWEDEMGVLQPQGALVVSDPNTGEVRAMVGGRDYGTHNMVNRVTSTRSPGSAMKPINVYAPAIEEGKLTPGTVID
DAPSSWEDGGDSYEPENFTNTFRGLTTVREALVDSLNIPAVRVYDDRLGTEIGAEYAEKFGVTTFTEGDRHNLAASIGGL
TKGVKPLELTEAYGTLANNGIRVENHTITKIEDRYGNTIYEREPDYDVVISEETSWLITDILQDVVTEGTAASLNLNFPA
AAKTGTSNNFRDAWLVGYTPNLVASFWLGYDYDDIQVQNRTHFTTNIIENVMTHALKDENPPEFQKPDGIEGPIEISEKS
GMRASDKTPSEYTTYEFFPEDQIPTGECDIFVEKKVCTAHKYSDNEEGDNSASNQEPKLASDNCPPEATETRVFLDRDDP
GFTDDGRTTYDASLMPPEEECTLHEKGDFPEDELEDEDNGLDFFDDDDDHKGEDLEDILEDEDQEDEEIDDEVEESHGDE
SSQDRDNGTDQDEDASEEKEEENEEELEDTNNNDNASDDENTS

Sequences:

>Translated_843_residues
MLIILFLGGGTAFGMVVAALQTVPEFEPGQLEATVPSKIKDPDGNEIVRLDREHQREEVSLDQVPDHVIEAFIAIEDARF
EEHFGFDVRGISRAAVNNFQETGNPFKGSQGGSTITQQLVKNTFLSPKRLLERKIHEAWLALHVERTYTKEEIMEYYLNY
ATYFHHNIYGIQAASNFYFDKDVSDLDVEEGALLAGIIRHPSRLSPHNNPDHSKQRQETVLHSMKSQDFISESEFNKALN
KELDEILASKPEKQYPYPHFIDYVINEEAKPILEDKIGSVESLPDNVQNAEDLLYHHGLTIHTTIDRELQSYSEDIMDNP
ENYPETWEDEMGVLQPQGALVVSDPNTGEVRAMVGGRDYGTHNMVNRVTSTRSPGSAMKPINVYAPAIEEGKLTPGTVID
DAPSSWEDGGDSYEPENFTNTFRGLTTVREALVDSLNIPAVRVYDDRLGTEIGAEYAEKFGVTTFTEGDRHNLAASIGGL
TKGVKPLELTEAYGTLANNGIRVENHTITKIEDRYGNTIYEREPDYDVVISEETSWLITDILQDVVTEGTAASLNLNFPA
AAKTGTSNNFRDAWLVGYTPNLVASFWLGYDYDDIQVQNRTHFTTNIIENVMTHALKDENPPEFQKPDGIEGPIEISEKS
GMRASDKTPSEYTTYEFFPEDQIPTGECDIFVEKKVCTAHKYSDNEEGDNSASNQEPKLASDNCPPEATETRVFLDRDDP
GFTDDGRTTYDASLMPPEEECTLHEKGDFPEDELEDEDNGLDFFDDDDDHKGEDLEDILEDEDQEDEEIDDEVEESHGDE
SSQDRDNGTDQDEDASEEKEEENEEELEDTNNNDNASDDENTS
>Mature_843_residues
MLIILFLGGGTAFGMVVAALQTVPEFEPGQLEATVPSKIKDPDGNEIVRLDREHQREEVSLDQVPDHVIEAFIAIEDARF
EEHFGFDVRGISRAAVNNFQETGNPFKGSQGGSTITQQLVKNTFLSPKRLLERKIHEAWLALHVERTYTKEEIMEYYLNY
ATYFHHNIYGIQAASNFYFDKDVSDLDVEEGALLAGIIRHPSRLSPHNNPDHSKQRQETVLHSMKSQDFISESEFNKALN
KELDEILASKPEKQYPYPHFIDYVINEEAKPILEDKIGSVESLPDNVQNAEDLLYHHGLTIHTTIDRELQSYSEDIMDNP
ENYPETWEDEMGVLQPQGALVVSDPNTGEVRAMVGGRDYGTHNMVNRVTSTRSPGSAMKPINVYAPAIEEGKLTPGTVID
DAPSSWEDGGDSYEPENFTNTFRGLTTVREALVDSLNIPAVRVYDDRLGTEIGAEYAEKFGVTTFTEGDRHNLAASIGGL
TKGVKPLELTEAYGTLANNGIRVENHTITKIEDRYGNTIYEREPDYDVVISEETSWLITDILQDVVTEGTAASLNLNFPA
AAKTGTSNNFRDAWLVGYTPNLVASFWLGYDYDDIQVQNRTHFTTNIIENVMTHALKDENPPEFQKPDGIEGPIEISEKS
GMRASDKTPSEYTTYEFFPEDQIPTGECDIFVEKKVCTAHKYSDNEEGDNSASNQEPKLASDNCPPEATETRVFLDRDDP
GFTDDGRTTYDASLMPPEEECTLHEKGDFPEDELEDEDNGLDFFDDDDDHKGEDLEDILEDEDQEDEEIDDEVEESHGDE
SSQDRDNGTDQDEDASEEKEEENEEELEDTNNNDNASDDENTS

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=585, Percent_Identity=28.7179487179487, Blast_Score=185, Evalue=1e-47,
Organism=Escherichia coli, GI1788867, Length=589, Percent_Identity=29.3718166383701, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI87082258, Length=321, Percent_Identity=33.3333333333333, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1789601, Length=166, Percent_Identity=38.5542168674699, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 94759; Mature: 94759

Theoretical pI: Translated: 3.99; Mature: 3.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIILFLGGGTAFGMVVAALQTVPEFEPGQLEATVPSKIKDPDGNEIVRLDREHQREEVS
CEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEECCHHHHHHHCC
LDQVPDHVIEAFIAIEDARFEEHFGFDVRGISRAAVNNFQETGNPFKGSQGGSTITQQLV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
KNTFLSPKRLLERKIHEAWLALHVERTYTKEEIMEYYLNYATYFHHNIYGIQAASNFYFD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEC
KDVSDLDVEEGALLAGIIRHPSRLSPHNNPDHSKQRQETVLHSMKSQDFISESEFNKALN
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELDEILASKPEKQYPYPHFIDYVINEEAKPILEDKIGSVESLPDNVQNAEDLLYHHGLT
HHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCHHHCCCHHHHHHHHHHHCCCE
IHTTIDRELQSYSEDIMDNPENYPETWEDEMGVLQPQGALVVSDPNTGEVRAMVGGRDYG
EEEHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCEEEEECCCCCC
THNMVNRVTSTRSPGSAMKPINVYAPAIEEGKLTPGTVIDDAPSSWEDGGDSYEPENFTN
HHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHH
TFRGLTTVREALVDSLNIPAVRVYDDRLGTEIGAEYAEKFGVTTFTEGDRHNLAASIGGL
HHHHHHHHHHHHHHHCCCCEEEEECHHHCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHH
TKGVKPLELTEAYGTLANNGIRVENHTITKIEDRYGNTIYEREPDYDVVISEETSWLITD
HCCCCCHHHHHHHHHHHCCCEEECCCEEEEEHHHHCCCCCCCCCCCEEEEECCCHHHHHH
ILQDVVTEGTAASLNLNFPAAAKTGTSNNFRDAWLVGYTPNLVASFWLGYDYDDIQVQNR
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCEEECCC
THFTTNIIENVMTHALKDENPPEFQKPDGIEGPIEISEKSGMRASDKTPSEYTTYEFFPE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEECCC
DQIPTGECDIFVEKKVCTAHKYSDNEEGDNSASNQEPKLASDNCPPEATETRVFLDRDDP
CCCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
GFTDDGRTTYDASLMPPEEECTLHEKGDFPEDELEDEDNGLDFFDDDDDHKGEDLEDILE
CCCCCCCEEECCCCCCCHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHC
DEDQEDEEIDDEVEESHGDESSQDRDNGTDQDEDASEEKEEENEEELEDTNNNDNASDDE
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
NTS
CCC
>Mature Secondary Structure
MLIILFLGGGTAFGMVVAALQTVPEFEPGQLEATVPSKIKDPDGNEIVRLDREHQREEVS
CEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEECCHHHHHHHCC
LDQVPDHVIEAFIAIEDARFEEHFGFDVRGISRAAVNNFQETGNPFKGSQGGSTITQQLV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
KNTFLSPKRLLERKIHEAWLALHVERTYTKEEIMEYYLNYATYFHHNIYGIQAASNFYFD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEC
KDVSDLDVEEGALLAGIIRHPSRLSPHNNPDHSKQRQETVLHSMKSQDFISESEFNKALN
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KELDEILASKPEKQYPYPHFIDYVINEEAKPILEDKIGSVESLPDNVQNAEDLLYHHGLT
HHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCHHHCCCHHHHHHHHHHHCCCE
IHTTIDRELQSYSEDIMDNPENYPETWEDEMGVLQPQGALVVSDPNTGEVRAMVGGRDYG
EEEHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCEEEEECCCCCC
THNMVNRVTSTRSPGSAMKPINVYAPAIEEGKLTPGTVIDDAPSSWEDGGDSYEPENFTN
HHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHH
TFRGLTTVREALVDSLNIPAVRVYDDRLGTEIGAEYAEKFGVTTFTEGDRHNLAASIGGL
HHHHHHHHHHHHHHHCCCCEEEEECHHHCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHH
TKGVKPLELTEAYGTLANNGIRVENHTITKIEDRYGNTIYEREPDYDVVISEETSWLITD
HCCCCCHHHHHHHHHHHCCCEEECCCEEEEEHHHHCCCCCCCCCCCEEEEECCCHHHHHH
ILQDVVTEGTAASLNLNFPAAAKTGTSNNFRDAWLVGYTPNLVASFWLGYDYDDIQVQNR
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCEEECCC
THFTTNIIENVMTHALKDENPPEFQKPDGIEGPIEISEKSGMRASDKTPSEYTTYEFFPE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEECCC
DQIPTGECDIFVEKKVCTAHKYSDNEEGDNSASNQEPKLASDNCPPEATETRVFLDRDDP
CCCCCCCCEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
GFTDDGRTTYDASLMPPEEECTLHEKGDFPEDELEDEDNGLDFFDDDDDHKGEDLEDILE
CCCCCCCEEECCCCCCCHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHC
DEDQEDEEIDDEVEESHGDESSQDRDNGTDQDEDASEEKEEENEEELEDTNNNDNASDDE
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
NTS
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]