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The map label for this gene is mrcA [H]

Identifier: 188586643

GI number: 188586643

Start: 2145344

End: 2146204

Strand: Reverse

Name: mrcA [H]

Synonym: Nther_2033

Alternate gene names: 188586643

Gene position: 2146204-2145344 (Counterclockwise)

Preceding gene: 188586644

Following gene: 188586642

Centisome position: 67.8

GC content: 37.4

Gene sequence:

>861_bases
ATGAAAAAAATATATAAAGGATTAATTGCCTTGGCGGCAGTATTTCTGTTAACTATTTTTGGGGGGGCAAGATTGCTTGA
AGTTTTAATTACTTATCCAGGCTCCCCCTATTATGTGGACTTTAAAGACCCCGACCCCCTATATGAAACGGAAGCAGAAA
TTCATACTCTAGAAAATGGAAATTTTGTGATTGGATTAAATGAACGAAATATTGAATATCATTCTTTAGAAGAGCTACCG
CCTGAGTTAATAGATGCTTTTATTGCCATTGAAGATAATCGTTTCTACAGGCATCGTGGTTATGATATTAAAGGAATTGC
CCGTGCTTTTTGGGTTAACCTAACTCACAGGGAAGTACGTCAAGGAGGCAGCACTATCACTCAACAATTAGCAAGAAATT
TATTTTTGGGTCATGATCAGACCCTTACTAGAAAAATATTGGAGTTTTCCTTGGCCGTTCAAATTGAGGATAGATTTACT
AAAGATGAAATTATGGAGATGTATCTCAATCAAGTTTATTTTGGAAATGGTAATTGGGGGGTTGAGCAAGCTGCTCAAGA
TTATTTTGACAAACCTGCCACTGAACTTGATTTGACGGAATCTTCAATGATAGCTGGCTTGGTCCAAGCTCCCAGCTACT
ATGCCCCCGTTACTAGCTGGGAACCGGCTGCTAATCGTCAAAAGATAGTGTTAAAAAGAATGGTTGAACTAGAGTTAATC
ACTGAAAGGGAAGCGCGAGAGGCATTATTCAATGAAGAGATTATCATAAATTTTCCTCGGGATATATTAAATTTGGCTTT
GGAAAAGTTGAATGTCGATAAATGTAGTGAGCATCAAAGACCTAACTATTATAGTGTCTAA

Upstream 100 bases:

>100_bases
GTACTTTCTACATTAAAAAAAGAGGGCATTATAAAAACTGATAAAGGTTATATTGCTGTTACTGATCCTGTGAAACTTAA
AGATAAGGTGATTGAGTGAC

Downstream 100 bases:

>100_bases
AATTTAAAGCGCTTAAATTTTTAAAGTTTTTGTAAATTTAAGGTAAATTTAGGTCCTGGGACTGCTTCAGATTGGGACTT
GAATATATCATGACAAACAA

Product: glycosyl transferase family 51

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKKIYKGLIALAAVFLLTIFGGARLLEVLITYPGSPYYVDFKDPDPLYETEAEIHTLENGNFVIGLNERNIEYHSLEELP
PELIDAFIAIEDNRFYRHRGYDIKGIARAFWVNLTHREVRQGGSTITQQLARNLFLGHDQTLTRKILEFSLAVQIEDRFT
KDEIMEMYLNQVYFGNGNWGVEQAAQDYFDKPATELDLTESSMIAGLVQAPSYYAPVTSWEPAANRQKIVLKRMVELELI
TEREAREALFNEEIIINFPRDILNLALEKLNVDKCSEHQRPNYYSV

Sequences:

>Translated_286_residues
MKKIYKGLIALAAVFLLTIFGGARLLEVLITYPGSPYYVDFKDPDPLYETEAEIHTLENGNFVIGLNERNIEYHSLEELP
PELIDAFIAIEDNRFYRHRGYDIKGIARAFWVNLTHREVRQGGSTITQQLARNLFLGHDQTLTRKILEFSLAVQIEDRFT
KDEIMEMYLNQVYFGNGNWGVEQAAQDYFDKPATELDLTESSMIAGLVQAPSYYAPVTSWEPAANRQKIVLKRMVELELI
TEREAREALFNEEIIINFPRDILNLALEKLNVDKCSEHQRPNYYSV
>Mature_286_residues
MKKIYKGLIALAAVFLLTIFGGARLLEVLITYPGSPYYVDFKDPDPLYETEAEIHTLENGNFVIGLNERNIEYHSLEELP
PELIDAFIAIEDNRFYRHRGYDIKGIARAFWVNLTHREVRQGGSTITQQLARNLFLGHDQTLTRKILEFSLAVQIEDRFT
KDEIMEMYLNQVYFGNGNWGVEQAAQDYFDKPATELDLTESSMIAGLVQAPSYYAPVTSWEPAANRQKIVLKRMVELELI
TEREAREALFNEEIIINFPRDILNLALEKLNVDKCSEHQRPNYYSV

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=170, Percent_Identity=50, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1789601, Length=216, Percent_Identity=32.8703703703704, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1786343, Length=142, Percent_Identity=38.7323943661972, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788867, Length=192, Percent_Identity=35.4166666666667, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 33033; Mature: 33033

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIYKGLIALAAVFLLTIFGGARLLEVLITYPGSPYYVDFKDPDPLYETEAEIHTLENG
CHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEECCC
NFVIGLNERNIEYHSLEELPPELIDAFIAIEDNRFYRHRGYDIKGIARAFWVNLTHREVR
CEEEEECCCCCCHHHHHHCCHHHHHHHHEEECCCEEECCCCCHHHHHHHHHHCCHHHHHH
QGGSTITQQLARNLFLGHDQTLTRKILEFSLAVQIEDRFTKDEIMEMYLNQVYFGNGNWG
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEHHCCCHHHHHHHHHHHEEECCCCCC
VEQAAQDYFDKPATELDLTESSMIAGLVQAPSYYAPVTSWEPAANRQKIVLKRMVELELI
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TEREAREALFNEEIIINFPRDILNLALEKLNVDKCSEHQRPNYYSV
HHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MKKIYKGLIALAAVFLLTIFGGARLLEVLITYPGSPYYVDFKDPDPLYETEAEIHTLENG
CHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCEEEECCC
NFVIGLNERNIEYHSLEELPPELIDAFIAIEDNRFYRHRGYDIKGIARAFWVNLTHREVR
CEEEEECCCCCCHHHHHHCCHHHHHHHHEEECCCEEECCCCCHHHHHHHHHHCCHHHHHH
QGGSTITQQLARNLFLGHDQTLTRKILEFSLAVQIEDRFTKDEIMEMYLNQVYFGNGNWG
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEHHCCCHHHHHHHHHHHEEECCCCCC
VEQAAQDYFDKPATELDLTESSMIAGLVQAPSYYAPVTSWEPAANRQKIVLKRMVELELI
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TEREAREALFNEEIIINFPRDILNLALEKLNVDKCSEHQRPNYYSV
HHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA