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The map label for this gene is smpB [H]

Identifier: 188586604

GI number: 188586604

Start: 2104029

End: 2104493

Strand: Reverse

Name: smpB [H]

Synonym: Nther_1994

Alternate gene names: 188586604

Gene position: 2104493-2104029 (Counterclockwise)

Preceding gene: 188586605

Following gene: 188586603

Centisome position: 66.48

GC content: 38.71

Gene sequence:

>465_bases
ATGGCAGAAGATGTAATGGTCTTAGCACGCAATAGAAAGGCAGAACACGAGTTTTTCATTGAAGAACGCTATGAAGCTGG
TATTGAACTTAAAGGGACAGAAGTGAAATCCTGTAAAAATCGCAGTGTAAATTTGAAGGACGGTTTTGCTAAGATTGAAA
AGGGCGAGATTTTCCTGTATAATGTTCATATAAGTCACTACAATGAAGGGAATCAATTCAATCACGAGCCCACCAGAACG
AGAAAACTTCTCCTTCACAAACGAGAGATCAAACGATTACTCGGTCTCACTCAACAGCGAAGATACACTTTAATTCCCCT
GACTTTTTACGTGAAGGATGGAATAATAAAGGTTGAATTGGCTCTTGCCAAGGGTAAACAAAAACACGATAAACGAGAAA
GCATCAAGCGAAAGACCATGGAAAGAGAGATGGAAAAGGCTCTAAAAGAAAAGCAAAAATATTGA

Upstream 100 bases:

>100_bases
TAATTTGCTTGGGCTAAGAACTAGGAATTTTGACTAATTCGTTCTTAATTAATATAATATAATTTCGGAAACAATTCAGA
GTATGCCAGGAGGCGATAGT

Downstream 100 bases:

>100_bases
CATTAAAGTTGTAATTTGTTATATATTATTGATGCTGGGGATGAACCCGGCATGAAGGCATTTAAACAACTAAAACATGG
GGGCGAGCTTGGATTCGACG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MAEDVMVLARNRKAEHEFFIEERYEAGIELKGTEVKSCKNRSVNLKDGFAKIEKGEIFLYNVHISHYNEGNQFNHEPTRT
RKLLLHKREIKRLLGLTQQRRYTLIPLTFYVKDGIIKVELALAKGKQKHDKRESIKRKTMEREMEKALKEKQKY

Sequences:

>Translated_154_residues
MAEDVMVLARNRKAEHEFFIEERYEAGIELKGTEVKSCKNRSVNLKDGFAKIEKGEIFLYNVHISHYNEGNQFNHEPTRT
RKLLLHKREIKRLLGLTQQRRYTLIPLTFYVKDGIIKVELALAKGKQKHDKRESIKRKTMEREMEKALKEKQKY
>Mature_153_residues
AEDVMVLARNRKAEHEFFIEERYEAGIELKGTEVKSCKNRSVNLKDGFAKIEKGEIFLYNVHISHYNEGNQFNHEPTRTR
KLLLHKREIKRLLGLTQQRRYTLIPLTFYVKDGIIKVELALAKGKQKHDKRESIKRKTMEREMEKALKEKQKY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=142, Percent_Identity=40.8450704225352, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 18333; Mature: 18201

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDVMVLARNRKAEHEFFIEERYEAGIELKGTEVKSCKNRSVNLKDGFAKIEKGEIFLY
CCCCEEEEECCCCCCHHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCCHHEEECCEEEEE
NVHISHYNEGNQFNHEPTRTRKLLLHKREIKRLLGLTQQRRYTLIPLTFYVKDGIIKVEL
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCEEEEEE
ALAKGKQKHDKRESIKRKTMEREMEKALKEKQKY
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEDVMVLARNRKAEHEFFIEERYEAGIELKGTEVKSCKNRSVNLKDGFAKIEKGEIFLY
CCCEEEEECCCCCCHHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCCHHEEECCEEEEE
NVHISHYNEGNQFNHEPTRTRKLLLHKREIKRLLGLTQQRRYTLIPLTFYVKDGIIKVEL
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCEEEEEE
ALAKGKQKHDKRESIKRKTMEREMEKALKEKQKY
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA