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The map label for this gene is asnB [H]

Identifier: 188586483

GI number: 188586483

Start: 1953932

End: 1955410

Strand: Direct

Name: asnB [H]

Synonym: Nther_1868

Alternate gene names: 188586483

Gene position: 1953932-1955410 (Clockwise)

Preceding gene: 188586482

Following gene: 188586487

Centisome position: 61.72

GC content: 33.81

Gene sequence:

>1479_bases
ATGAGTGGAATTGTTGCTATTAAAGGTACCCAAGCTTATAAAGATACTATGGTCAAAATGTTAGACAAACTTAAACACCG
AGGTCCTCAAAAACAAAATATATTTCCAAGCTATGCAAGCAATGTTTTGATCGGAGAATGTAATCTAGAATACGAATTGA
GTAAAATTGATGAAAGCCAAAATCCTATAGACAACAAAAATCACAATATTAATTTAGCACTAGATGGCGAACCCCTCATG
GAAGGTAAGCGCCTATCAAGAAATGATTTAATTAAATTATTTCAAAGGGAAGGTCCTGAATTTGTAAAGTTTTTGGGAGA
TGGATTTGCTTTGATTTATACTGATGGCAAAGAACTCTATGCTGCAAGAGACATTTTTGGTAGAAGACCATTATATTACG
GTCATGATTCTTCAAACAATATTTACTTTGCATCAGAAATGAAAGCTTTGACAGAAATATGTGAAGAAATAAAAATTTTC
CCTCCAGGTTATTATTATTCTCAAGATGATGGTTTTAACAAGTATACCAGTATACCTCATTTCGAATCAAATATTGATGA
AGAACCAGGTTCTAAGGACTATTATGAAAAGTCTGCTCGGTTAAAGTATTTGTTAATTAAAGCAGTAGCCGACAGTTGGG
AAACAGGCAAAGAGCCTGGCATTTTTTTGAGTGGAGGAGTTGATAGTTCAGTAGTAGCTGCAGCTTTAGCCAATTTAACT
GATAAACCTATTCACACCTTCGCTGTAGGGATTGAGGACAGTGAAGATATAAAAAATGCCGAAGAAGTTAGTCAATATCT
TGGTACAGAACATACGGTTCTTACTTATACAGAAGAAGATATGTTTGAAGTGTTACCAGAGGTGATTTACTATCTTGAAT
CCTTTGATGTTGAGCTTGTTAACAGTTCTATTGCCAATTATTTGGTCTCGAAACTCGCAAAATCTAATAATTTTGAAATC
ACACTATCAGGTGAAGGTGCTGATGAACTTTTTGGGGGATATCATCATTTAAAAGATTGTAAAAGTGATGAACAGTTAAA
TGAAGCTTTAGAAGGATTATTGAAAGGTTTACATAATGGTGGATTGCAAAGAGTTGATCGAATGACAAATGCTCATTCAT
TAAATTGTCGAATGCCTTTCTTACACTCTTATATCATAAATTACGCCCTAAAGATCCCCTGTGATTGGAAAATTTCAGGA
AATGGTATGGAAAAACTAATTTTACGTCAGGCCTTTGATGGAGACTTACCTGAGCAGGTTTTATGGCGCAAAAAAGCCCA
GTTTGGCATTGGATCAGGAAACGAAAAAGCTATGGAAAAAATCATAGACTCGGAAATCACTGACCAAGAATATTATGATA
ACAAAGAAAAACACGGAATTGATTTTAAATCAAAAGAAGAATATTTCTATTTTAAAATTTTTCATAAATTTTTTCCCGAA
AAATCTCAAACTAAAACTGTCAACCGCTGGTTAGTGTAA

Upstream 100 bases:

>100_bases
ACAACCCTTAAAAAGTATAATATAAAAAATATCATTGGAGAATTCTAAAAATAGCACTATAATAACTTAACTTCTTAGAA
TTTAATTAGGAGTGAACGAA

Downstream 100 bases:

>100_bases
ATATTCTAATGATTTTCTAATTTAGCATAAAAAAGGACAGTGAATCTATGTATCTTTGATTCACTGTCCTTTTTTTATTA
ATTTTTTTCAAAAAGTGTTA

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSGIVAIKGTQAYKDTMVKMLDKLKHRGPQKQNIFPSYASNVLIGECNLEYELSKIDESQNPIDNKNHNINLALDGEPLM
EGKRLSRNDLIKLFQREGPEFVKFLGDGFALIYTDGKELYAARDIFGRRPLYYGHDSSNNIYFASEMKALTEICEEIKIF
PPGYYYSQDDGFNKYTSIPHFESNIDEEPGSKDYYEKSARLKYLLIKAVADSWETGKEPGIFLSGGVDSSVVAAALANLT
DKPIHTFAVGIEDSEDIKNAEEVSQYLGTEHTVLTYTEEDMFEVLPEVIYYLESFDVELVNSSIANYLVSKLAKSNNFEI
TLSGEGADELFGGYHHLKDCKSDEQLNEALEGLLKGLHNGGLQRVDRMTNAHSLNCRMPFLHSYIINYALKIPCDWKISG
NGMEKLILRQAFDGDLPEQVLWRKKAQFGIGSGNEKAMEKIIDSEITDQEYYDNKEKHGIDFKSKEEYFYFKIFHKFFPE
KSQTKTVNRWLV

Sequences:

>Translated_492_residues
MSGIVAIKGTQAYKDTMVKMLDKLKHRGPQKQNIFPSYASNVLIGECNLEYELSKIDESQNPIDNKNHNINLALDGEPLM
EGKRLSRNDLIKLFQREGPEFVKFLGDGFALIYTDGKELYAARDIFGRRPLYYGHDSSNNIYFASEMKALTEICEEIKIF
PPGYYYSQDDGFNKYTSIPHFESNIDEEPGSKDYYEKSARLKYLLIKAVADSWETGKEPGIFLSGGVDSSVVAAALANLT
DKPIHTFAVGIEDSEDIKNAEEVSQYLGTEHTVLTYTEEDMFEVLPEVIYYLESFDVELVNSSIANYLVSKLAKSNNFEI
TLSGEGADELFGGYHHLKDCKSDEQLNEALEGLLKGLHNGGLQRVDRMTNAHSLNCRMPFLHSYIINYALKIPCDWKISG
NGMEKLILRQAFDGDLPEQVLWRKKAQFGIGSGNEKAMEKIIDSEITDQEYYDNKEKHGIDFKSKEEYFYFKIFHKFFPE
KSQTKTVNRWLV
>Mature_491_residues
SGIVAIKGTQAYKDTMVKMLDKLKHRGPQKQNIFPSYASNVLIGECNLEYELSKIDESQNPIDNKNHNINLALDGEPLME
GKRLSRNDLIKLFQREGPEFVKFLGDGFALIYTDGKELYAARDIFGRRPLYYGHDSSNNIYFASEMKALTEICEEIKIFP
PGYYYSQDDGFNKYTSIPHFESNIDEEPGSKDYYEKSARLKYLLIKAVADSWETGKEPGIFLSGGVDSSVVAAALANLTD
KPIHTFAVGIEDSEDIKNAEEVSQYLGTEHTVLTYTEEDMFEVLPEVIYYLESFDVELVNSSIANYLVSKLAKSNNFEIT
LSGEGADELFGGYHHLKDCKSDEQLNEALEGLLKGLHNGGLQRVDRMTNAHSLNCRMPFLHSYIINYALKIPCDWKISGN
GMEKLILRQAFDGDLPEQVLWRKKAQFGIGSGNEKAMEKIIDSEITDQEYYDNKEKHGIDFKSKEEYFYFKIFHKFFPEK
SQTKTVNRWLV

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=534, Percent_Identity=28.6516853932584, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI168229252, Length=534, Percent_Identity=28.6516853932584, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI168229250, Length=534, Percent_Identity=28.6516853932584, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI296010852, Length=430, Percent_Identity=30.4651162790698, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI296010850, Length=430, Percent_Identity=30.4651162790698, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI296010848, Length=430, Percent_Identity=30.4651162790698, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1786889, Length=497, Percent_Identity=31.58953722334, Blast_Score=229, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17560178, Length=383, Percent_Identity=34.2036553524804, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI71993933, Length=422, Percent_Identity=31.042654028436, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI25147557, Length=422, Percent_Identity=31.042654028436, Blast_Score=204, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI25147560, Length=174, Percent_Identity=31.6091954022989, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321563, Length=561, Percent_Identity=27.9857397504456, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6325403, Length=562, Percent_Identity=28.1138790035587, Blast_Score=210, Evalue=4e-55,
Organism=Drosophila melanogaster, GI45553209, Length=504, Percent_Identity=29.5634920634921, Blast_Score=216, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 56092; Mature: 55961

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIVAIKGTQAYKDTMVKMLDKLKHRGPQKQNIFPSYASNVLIGECNLEYELSKIDESQ
CCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEECCCEEEHHHCCCCC
NPIDNKNHNINLALDGEPLMEGKRLSRNDLIKLFQREGPEFVKFLGDGFALIYTDGKELY
CCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCEEEEEECCHHHH
AARDIFGRRPLYYGHDSSNNIYFASEMKALTEICEEIKIFPPGYYYSQDDGFNKYTSIPH
HHHHHHCCCCEEECCCCCCCEEEHHHHHHHHHHHHHCEECCCCCCCCCCCCCCHHCCCCC
FESNIDEEPGSKDYYEKSARLKYLLIKAVADSWETGKEPGIFLSGGVDSSVVAAALANLT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCC
DKPIHTFAVGIEDSEDIKNAEEVSQYLGTEHTVLTYTEEDMFEVLPEVIYYLESFDVELV
CCCCEEEEECCCCCHHHCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHCCHHHH
NSSIANYLVSKLAKSNNFEITLSGEGADELFGGYHHLKDCKSDEQLNEALEGLLKGLHNG
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCC
GLQRVDRMTNAHSLNCRMPFLHSYIINYALKIPCDWKISGNGMEKLILRQAFDGDLPEQV
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCEEECCCCHHHHHHHHHCCCCCCHHH
LWRKKAQFGIGSGNEKAMEKIIDSEITDQEYYDNKEKHGIDFKSKEEYFYFKIFHKFFPE
HHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHCCCHHCCCCCCCCCCEEHHHHHHHHCCC
KSQTKTVNRWLV
CCCCHHHHHCCC
>Mature Secondary Structure 
SGIVAIKGTQAYKDTMVKMLDKLKHRGPQKQNIFPSYASNVLIGECNLEYELSKIDESQ
CCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEECCCEEEHHHCCCCC
NPIDNKNHNINLALDGEPLMEGKRLSRNDLIKLFQREGPEFVKFLGDGFALIYTDGKELY
CCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCEEEEEECCHHHH
AARDIFGRRPLYYGHDSSNNIYFASEMKALTEICEEIKIFPPGYYYSQDDGFNKYTSIPH
HHHHHHCCCCEEECCCCCCCEEEHHHHHHHHHHHHHCEECCCCCCCCCCCCCCHHCCCCC
FESNIDEEPGSKDYYEKSARLKYLLIKAVADSWETGKEPGIFLSGGVDSSVVAAALANLT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCC
DKPIHTFAVGIEDSEDIKNAEEVSQYLGTEHTVLTYTEEDMFEVLPEVIYYLESFDVELV
CCCCEEEEECCCCCHHHCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHCCHHHH
NSSIANYLVSKLAKSNNFEITLSGEGADELFGGYHHLKDCKSDEQLNEALEGLLKGLHNG
HHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCC
GLQRVDRMTNAHSLNCRMPFLHSYIINYALKIPCDWKISGNGMEKLILRQAFDGDLPEQV
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHEECCCCEEECCCCHHHHHHHHHCCCCCCHHH
LWRKKAQFGIGSGNEKAMEKIIDSEITDQEYYDNKEKHGIDFKSKEEYFYFKIFHKFFPE
HHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHCCCHHCCCCCCCCCCEEHHHHHHHHCCC
KSQTKTVNRWLV
CCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]