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The map label for this gene is tgt

Identifier: 188586406

GI number: 188586406

Start: 1855913

End: 1857025

Strand: Reverse

Name: tgt

Synonym: Nther_1791

Alternate gene names: 188586406

Gene position: 1857025-1855913 (Counterclockwise)

Preceding gene: 188586407

Following gene: 188586405

Centisome position: 58.66

GC content: 36.12

Gene sequence:

>1113_bases
TTGGCTATAAGTTATCAATTAGAACAAACATCATCGGAATCCAGGGCTAGACTGGGAAAGCTCAAAACTCCTCGTGGTGA
AATTCAAACTCCAGTTTTTATGCCTGTGGGAACCCAAGCTACTGTAAAAACCATGACACCAGAAGAGTTAAAGAACTTAG
ACGCTGAAATAATATTAGGTAATACGTATCATTTGCATTTGCGACCAGGAAATGACATTGTTAGGGAAGCAGACGGACTT
CACAAATTCATGAATTGGGATAGACCCATACTTACTGATAGTGGGGGGTTTCAGGTATTTAGTCTGGGTAAACTAAGACA
AATATCAGAACAGGGTGTTGAATTTCGTTCACATATAGACGGTTCAAAACTGTTTATGACACCTGAAAAATCTATTGAAA
TTCAGGAGGATTTGGGTTCTGACATAATGATGGTCTTTGATGAATGTCCACCCTATCCAGCCGAATACGATTATGTTAAA
GAATCAATGGACCGTACTATCCGGTGGAGCAAAAGATGCTTACAACATCAAAAACACCCTGAAAAACAAGCCTTGTTTGG
TATAGTTCAGGGTGGTATGTATCCCGAATTAAGAAAAGAAAGTGCTTTAAAAACAACAGAATTAGATTTCCCAGGTTATG
CAGTAGGTGGATTAAGTGTAGGTGAACCTAAAGAAATGATGCTTGAAGTACTTAATACAACAATTCCTTATCTACCGGAA
GAAAAGCCCCGATATTTGATGGGGGTTGGTACACCTGATTATATAATAGAAGCAGTTAGAATGGGTATAGATATGTTTGA
CTGTGTTTATCCTACAAGAGTAGCACGAAATGGTACTGCTATGACTAGATTTGGGAATTTAACTGTTAGAAATGCTGTAT
TTCAAAGAGATTTTCAACCAATAGAAGAAGATTGTGATTGTTATGTATGTCAAAATTATTCAAGAGCTTATCTAAGGCAC
TTAATAAAAGCAAATGAAATCCTTGGATTTAGATTACTGACTTGGCATAATTTGTTTTTTCTGATAAAGTTAATAAAGGA
ACTACGTCAGGCAATAGCAGATGATAATTTTCTAGCCTGGCGGGATTCTTTTTATAAAAATTATCAAAATTAA

Upstream 100 bases:

>100_bases
ATCAAGAATTCATATCTAAAGTTAGAATAAAAAGATTGAACAATTAAAATGTAATGATTTTAGATTTTTTTAATGAAATG
ACTACAGGCAGGTGAAATTA

Downstream 100 bases:

>100_bases
TATTTAATTAAGGAGGAATACTCTCATGCCAGAAGTAGTGGGTTCGTTTTTACCGCTGATTATTTTAATTGCAATTTTTT
ACTTCTTGCTTATCAGACCA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MAISYQLEQTSSESRARLGKLKTPRGEIQTPVFMPVGTQATVKTMTPEELKNLDAEIILGNTYHLHLRPGNDIVREADGL
HKFMNWDRPILTDSGGFQVFSLGKLRQISEQGVEFRSHIDGSKLFMTPEKSIEIQEDLGSDIMMVFDECPPYPAEYDYVK
ESMDRTIRWSKRCLQHQKHPEKQALFGIVQGGMYPELRKESALKTTELDFPGYAVGGLSVGEPKEMMLEVLNTTIPYLPE
EKPRYLMGVGTPDYIIEAVRMGIDMFDCVYPTRVARNGTAMTRFGNLTVRNAVFQRDFQPIEEDCDCYVCQNYSRAYLRH
LIKANEILGFRLLTWHNLFFLIKLIKELRQAIADDNFLAWRDSFYKNYQN

Sequences:

>Translated_370_residues
MAISYQLEQTSSESRARLGKLKTPRGEIQTPVFMPVGTQATVKTMTPEELKNLDAEIILGNTYHLHLRPGNDIVREADGL
HKFMNWDRPILTDSGGFQVFSLGKLRQISEQGVEFRSHIDGSKLFMTPEKSIEIQEDLGSDIMMVFDECPPYPAEYDYVK
ESMDRTIRWSKRCLQHQKHPEKQALFGIVQGGMYPELRKESALKTTELDFPGYAVGGLSVGEPKEMMLEVLNTTIPYLPE
EKPRYLMGVGTPDYIIEAVRMGIDMFDCVYPTRVARNGTAMTRFGNLTVRNAVFQRDFQPIEEDCDCYVCQNYSRAYLRH
LIKANEILGFRLLTWHNLFFLIKLIKELRQAIADDNFLAWRDSFYKNYQN
>Mature_369_residues
AISYQLEQTSSESRARLGKLKTPRGEIQTPVFMPVGTQATVKTMTPEELKNLDAEIILGNTYHLHLRPGNDIVREADGLH
KFMNWDRPILTDSGGFQVFSLGKLRQISEQGVEFRSHIDGSKLFMTPEKSIEIQEDLGSDIMMVFDECPPYPAEYDYVKE
SMDRTIRWSKRCLQHQKHPEKQALFGIVQGGMYPELRKESALKTTELDFPGYAVGGLSVGEPKEMMLEVLNTTIPYLPEE
KPRYLMGVGTPDYIIEAVRMGIDMFDCVYPTRVARNGTAMTRFGNLTVRNAVFQRDFQPIEEDCDCYVCQNYSRAYLRHL
IKANEILGFRLLTWHNLFFLIKLIKELRQAIADDNFLAWRDSFYKNYQN

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=352, Percent_Identity=46.5909090909091, Blast_Score=347, Evalue=7e-96,
Organism=Homo sapiens, GI13375872, Length=290, Percent_Identity=26.2068965517241, Blast_Score=115, Evalue=6e-26,
Organism=Escherichia coli, GI1786607, Length=362, Percent_Identity=53.8674033149171, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17544680, Length=355, Percent_Identity=47.0422535211268, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI25146502, Length=212, Percent_Identity=26.8867924528302, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19920510, Length=366, Percent_Identity=45.3551912568306, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI21355311, Length=217, Percent_Identity=29.4930875576037, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_NATTJ (B2A5K8)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917951.1
- ProteinModelPortal:   B2A5K8
- SMR:   B2A5K8
- GeneID:   6315254
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1791
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42655; Mature: 42523

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: ACT_SITE 93-93 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISYQLEQTSSESRARLGKLKTPRGEIQTPVFMPVGTQATVKTMTPEELKNLDAEIILG
CCEEEECHHCCCHHHHHHCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHCCCCEEEEC
NTYHLHLRPGNDIVREADGLHKFMNWDRPILTDSGGFQVFSLGKLRQISEQGVEFRSHID
CEEEEEEECCCHHHHHHHHHHHHHCCCCCEEECCCCEEEEECHHHHHHHHHCHHHHHCCC
GSKLFMTPEKSIEIQEDLGSDIMMVFDECPPYPAEYDYVKESMDRTIRWSKRCLQHQKHP
CCEEEECCCCCEEEHHHCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EKQALFGIVQGGMYPELRKESALKTTELDFPGYAVGGLSVGEPKEMMLEVLNTTIPYLPE
HHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCC
EKPRYLMGVGTPDYIIEAVRMGIDMFDCVYPTRVARNGTAMTRFGNLTVRNAVFQRDFQP
CCCCEEEECCCHHHHHHHHHHCCHHHHHHCHHHHHCCCCEEEEECCCHHHHHHHHHCCCC
IEEDCDCYVCQNYSRAYLRHLIKANEILGFRLLTWHNLFFLIKLIKELRQAIADDNFLAW
HHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEH
RDSFYKNYQN
HHHHHHCCCC
>Mature Secondary Structure 
AISYQLEQTSSESRARLGKLKTPRGEIQTPVFMPVGTQATVKTMTPEELKNLDAEIILG
CEEEECHHCCCHHHHHHCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHCCCCEEEEC
NTYHLHLRPGNDIVREADGLHKFMNWDRPILTDSGGFQVFSLGKLRQISEQGVEFRSHID
CEEEEEEECCCHHHHHHHHHHHHHCCCCCEEECCCCEEEEECHHHHHHHHHCHHHHHCCC
GSKLFMTPEKSIEIQEDLGSDIMMVFDECPPYPAEYDYVKESMDRTIRWSKRCLQHQKHP
CCEEEECCCCCEEEHHHCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EKQALFGIVQGGMYPELRKESALKTTELDFPGYAVGGLSVGEPKEMMLEVLNTTIPYLPE
HHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCC
EKPRYLMGVGTPDYIIEAVRMGIDMFDCVYPTRVARNGTAMTRFGNLTVRNAVFQRDFQP
CCCCEEEECCCHHHHHHHHHHCCHHHHHHCHHHHHCCCCEEEEECCCHHHHHHHHHCCCC
IEEDCDCYVCQNYSRAYLRHLIKANEILGFRLLTWHNLFFLIKLIKELRQAIADDNFLAW
HHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEH
RDSFYKNYQN
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA