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The map label for this gene is rocG [H]

Identifier: 188586111

GI number: 188586111

Start: 1556677

End: 1557927

Strand: Direct

Name: rocG [H]

Synonym: Nther_1485

Alternate gene names: 188586111

Gene position: 1556677-1557927 (Clockwise)

Preceding gene: 188586110

Following gene: 188586112

Centisome position: 49.18

GC content: 39.49

Gene sequence:

>1251_bases
ATGTCTGAAAACCTAAACATTTATCACATTGTCCAGAGGCAAATAAAAGAAGCCGTGGAAATGTTAGGGGTTGACTTTAC
TGTATATGAAATCCTGAAACAGCCTCAAAATGTATTAGAAGTATCTTTCCCCATCAAAATGGATGATGGTTCAATAAAAG
TTTTCAGAGGCCTAAGATCACAGCACTGTGACGTTTTAGGACCATACAAAGGTGGTCTTAGATTTCATCCAACTGTAGAA
ATGGATGAGTCCAAAGCCTTATCAATGTGGATGACCTTTAAATGTGCCGTGGTAGGAGTTCCTTATGGTGGCGCCAAAGG
TGGAGTTGAATGTAACCCTAAGGATCTGAGCCAGCGGGAAATGGAACGGCTCAGCCGTGGTTTCATTAAAAAAATAGCAA
ACTTTGTAGGGCCTGAAAAAGATATTCCGGCCCCGGATGTTTATACTAATCCCCAGGTAATGGCCTGGATGATGGACGAA
TTTAGTAATGTAAGAGGATATAATAACTTTGGCTTAATTACAGGAAAGCCAATCATAGTCGGAGGTTCTAAAGGTAGAAG
TGAAGCTACGGGTCGTGGTTGCGTTTATGTAACCAGGGAAGCTGTTAAAGAAATGGATTGGAACATGGAAGATATGAGAG
TTGTAGTTCAAGGATTCGGGAATGCTGGACGGATCGCAGCTAAACTACTACACGATATGGGAGCTACTATAGTTGGCACC
AACGATTCAATCGCAGGAGTATATAATAAAGAAGGAATTAACCCTTATGATTTAGAAAACTTTAAGGAGGAGACTGGATC
TGTAAAAGATTATCCTGGCTCAGAGCATGTTACTAATGACGAACTATTGACTGCAGATTGTGATATTTTAATCCCTGCAG
CTTTGGAAAACCAAATTACTCAAGCTAATGCAGGGCAAATTAAAGCTAAAATTATTTCTGAAGCAGCCAATGGACCTACT
ACACCAGATGCTGATAAAATATTGTATGAAAATGGTATACTAACAATACCAGATATATTAGCTAACGCTGGTGGCGTGAC
AGTTAGTTACTTCGAATGGGTACAAAACTTACAGAATTTCTACTGGACGGAAGATGAAGTGAATAATAGAATGGAAGAGA
TGATGGTCAGTGCATTCAAAAACTGCTTTAAAGCCAGAGAAGGATATGGAGTACACATGAGGACAGCTGCTTATCTAGTA
GCTATTCAAAGATTGGCTAATGCTATGAAAATTCGAGGATGGTTAAACTAA

Upstream 100 bases:

>100_bases
GAACCGAGGAACTTAACTGGGAAGCCTTTCAAAAAGGCTATCAGCTAGCAAAAGAAAAGGATAACTAAATATATTTAAAT
ATTTTAAGGAGGAGATTTAA

Downstream 100 bases:

>100_bases
TTTTGTTTAATCTTGGTAGACTGAAATTAGGTTAATTTAAGAAATTTTTTGTAATTGAACTTTCGTAAAATCATCTAAAC
AGGAGGGATCTTTTTGACCA

Product: glutamate dehydrogenase (NAD)

Products: NA

Alternate protein names: NAD-GDH [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSENLNIYHIVQRQIKEAVEMLGVDFTVYEILKQPQNVLEVSFPIKMDDGSIKVFRGLRSQHCDVLGPYKGGLRFHPTVE
MDESKALSMWMTFKCAVVGVPYGGAKGGVECNPKDLSQREMERLSRGFIKKIANFVGPEKDIPAPDVYTNPQVMAWMMDE
FSNVRGYNNFGLITGKPIIVGGSKGRSEATGRGCVYVTREAVKEMDWNMEDMRVVVQGFGNAGRIAAKLLHDMGATIVGT
NDSIAGVYNKEGINPYDLENFKEETGSVKDYPGSEHVTNDELLTADCDILIPAALENQITQANAGQIKAKIISEAANGPT
TPDADKILYENGILTIPDILANAGGVTVSYFEWVQNLQNFYWTEDEVNNRMEEMMVSAFKNCFKAREGYGVHMRTAAYLV
AIQRLANAMKIRGWLN

Sequences:

>Translated_416_residues
MSENLNIYHIVQRQIKEAVEMLGVDFTVYEILKQPQNVLEVSFPIKMDDGSIKVFRGLRSQHCDVLGPYKGGLRFHPTVE
MDESKALSMWMTFKCAVVGVPYGGAKGGVECNPKDLSQREMERLSRGFIKKIANFVGPEKDIPAPDVYTNPQVMAWMMDE
FSNVRGYNNFGLITGKPIIVGGSKGRSEATGRGCVYVTREAVKEMDWNMEDMRVVVQGFGNAGRIAAKLLHDMGATIVGT
NDSIAGVYNKEGINPYDLENFKEETGSVKDYPGSEHVTNDELLTADCDILIPAALENQITQANAGQIKAKIISEAANGPT
TPDADKILYENGILTIPDILANAGGVTVSYFEWVQNLQNFYWTEDEVNNRMEEMMVSAFKNCFKAREGYGVHMRTAAYLV
AIQRLANAMKIRGWLN
>Mature_415_residues
SENLNIYHIVQRQIKEAVEMLGVDFTVYEILKQPQNVLEVSFPIKMDDGSIKVFRGLRSQHCDVLGPYKGGLRFHPTVEM
DESKALSMWMTFKCAVVGVPYGGAKGGVECNPKDLSQREMERLSRGFIKKIANFVGPEKDIPAPDVYTNPQVMAWMMDEF
SNVRGYNNFGLITGKPIIVGGSKGRSEATGRGCVYVTREAVKEMDWNMEDMRVVVQGFGNAGRIAAKLLHDMGATIVGTN
DSIAGVYNKEGINPYDLENFKEETGSVKDYPGSEHVTNDELLTADCDILIPAALENQITQANAGQIKAKIISEAANGPTT
PDADKILYENGILTIPDILANAGGVTVSYFEWVQNLQNFYWTEDEVNNRMEEMMVSAFKNCFKAREGYGVHMRTAAYLVA
IQRLANAMKIRGWLN

Specific function: Catabolic GlutDH involved in the utilization of glutamate and other amino acids of the glutamate family [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=348, Percent_Identity=45.1149425287356, Blast_Score=311, Evalue=5e-85,
Organism=Homo sapiens, GI4885281, Length=348, Percent_Identity=45.1149425287356, Blast_Score=310, Evalue=1e-84,
Organism=Escherichia coli, GI1788059, Length=401, Percent_Identity=32.1695760598504, Blast_Score=194, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17544676, Length=409, Percent_Identity=39.6088019559902, Blast_Score=282, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6319256, Length=403, Percent_Identity=29.5285359801489, Blast_Score=156, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6324951, Length=419, Percent_Identity=29.3556085918854, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI45549226, Length=346, Percent_Identity=43.6416184971098, Blast_Score=292, Evalue=3e-79,
Organism=Drosophila melanogaster, GI45553475, Length=346, Percent_Identity=43.6416184971098, Blast_Score=291, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24649283, Length=348, Percent_Identity=41.0919540229885, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24649460, Length=285, Percent_Identity=44.2105263157895, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 46226; Mature: 46095

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENLNIYHIVQRQIKEAVEMLGVDFTVYEILKQPQNVLEVSFPIKMDDGSIKVFRGLRS
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHEEEECCEEECCCCHHHHHHHHH
QHCDVLGPYKGGLRFHPTVEMDESKALSMWMTFKCAVVGVPYGGAKGGVECNPKDLSQRE
CCCCEECCCCCCEEECCCCCCCCCHHHHHHEEEEEEEEECCCCCCCCCCCCCHHHHHHHH
MERLSRGFIKKIANFVGPEKDIPAPDVYTNPQVMAWMMDEFSNVRGYNNFGLITGKPIIV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCEEEE
GGSKGRSEATGRGCVYVTREAVKEMDWNMEDMRVVVQGFGNAGRIAAKLLHDMGATIVGT
ECCCCCCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEC
NDSIAGVYNKEGINPYDLENFKEETGSVKDYPGSEHVTNDELLTADCDILIPAALENQIT
CCCEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEECHHHHHHHH
QANAGQIKAKIISEAANGPTTPDADKILYENGILTIPDILANAGGVTVSYFEWVQNLQNF
CCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCEECHHHHCCCCCCCHHHHHHHHHHHHH
YWTEDEVNNRMEEMMVSAFKNCFKAREGYGVHMRTAAYLVAIQRLANAMKIRGWLN
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SENLNIYHIVQRQIKEAVEMLGVDFTVYEILKQPQNVLEVSFPIKMDDGSIKVFRGLRS
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHEEEECCEEECCCCHHHHHHHHH
QHCDVLGPYKGGLRFHPTVEMDESKALSMWMTFKCAVVGVPYGGAKGGVECNPKDLSQRE
CCCCEECCCCCCEEECCCCCCCCCHHHHHHEEEEEEEEECCCCCCCCCCCCCHHHHHHHH
MERLSRGFIKKIANFVGPEKDIPAPDVYTNPQVMAWMMDEFSNVRGYNNFGLITGKPIIV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCEEEE
GGSKGRSEATGRGCVYVTREAVKEMDWNMEDMRVVVQGFGNAGRIAAKLLHDMGATIVGT
ECCCCCCCCCCCCEEEEEHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEC
NDSIAGVYNKEGINPYDLENFKEETGSVKDYPGSEHVTNDELLTADCDILIPAALENQIT
CCCEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEECHHHHHHHH
QANAGQIKAKIISEAANGPTTPDADKILYENGILTIPDILANAGGVTVSYFEWVQNLQNF
CCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCEECHHHHCCCCCCCHHHHHHHHHHHHH
YWTEDEVNNRMEEMMVSAFKNCFKAREGYGVHMRTAAYLVAIQRLANAMKIRGWLN
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 7565595; 9829940 [H]