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The map label for this gene is pnp

Identifier: 188586076

GI number: 188586076

Start: 1516990

End: 1519146

Strand: Direct

Name: pnp

Synonym: Nther_1450

Alternate gene names: 188586076

Gene position: 1516990-1519146 (Clockwise)

Preceding gene: 188586075

Following gene: 188586077

Centisome position: 47.92

GC content: 39.59

Gene sequence:

>2157_bases
ATGCCAACAACTGTTTCAGAAGAAATAGCAGGATATCAGCTCACCCTTGAGACAGGGGAATTAGCTAAACAGGCTAATGG
TGCTGTTAAAGTTCAGTACGGCAATACAGTAGTGCTGGTAACTGCTACTGCTTCAAAAGAACCAAAAGATGATTTGAATT
TCTTTCCACTAACAGTGGATTACGAAGAAAGACTTTACGCTGTTGGAAAGATACCCGGTGGTTTTATTAAACGTGAAGGT
AAGCCTACGGAAAAGGCAACTCTAACTGCTAGATTAACTGACCGACCAATTCGTCCATTATTCCCTGATGGATTTAGAAA
TCCTGTTCACATAGTCAGTACAGTCTTGAGTGTAGATCAAAACTGCCCCCCTGAAATTGCCAGCATAATCGGAGCTTCTG
CTGCCCTGTCAATTTCGGACATTCCCTTTGACGGACCTATAGCATCAGTCAAGGTTGGAAAAGTTAATGATGAAATTGTG
GTCACACCAGATGTGGATGAACACGAAGAAAGTCAACTTGACTTGGTAGTAGCCGGAACTAAGGATGCCATTATGATGGT
TGAAGCCGAAGCCAACGAGTTACCCGAAGATGAAATGTTAGAAGCAATTATGAAAGCTCATGAAGAAATCAAAAAGATAA
TTACCATGCAAGAGCAGCTAGTAGAACAAGTTGGCCAGAAAAAGATGGAAGTCGAGCTAGACCTTCCTAGTGACGAACTT
GTTTCCGAAGTAGAAGAACTTGCCCTGGAAGATATTGAGAAAGTTCTACAAATAAAGGATAAACTAGAACGTGAAGATGC
TATGGATGAAGCAAAACAAAAAGTCGTTGATCACTTATTGGAGAAATACAACTCTGAAGAGAATGAAGACGAAGAAGAGG
AACTCAAAGAAAAGCATATTAAAAGCGCTTTTGATTCGATACTGAAACGAGAAATGCGCTCACGTATCATTCATGAAAAT
TCAAGGCCTGATGGAAGAGGTCAAAAAGAAATTCGACCTGTAACTTGTGATGTAGATTTACTACCTAACACTCATGGTTC
TGGACTATTTACTAGAGGTCAAACTCAAGTTTTAAATGTGTGTACATTAGGAGCTTTAGGTGATGTACAAATACTTGATG
GATTGGATATTGAAGAGTCCAAACGTTATATGCATCACTACAACTTCCCACCATATAGTGTAGGAGAAGCTGGCTTTATG
AAAGGTCCTGGAAGACGTGAAATTGGTCATGGTGCCTTAGCAGAACGAGCCTTAAAGCCAATGATACCTACCGAAAAAGA
CTTCCCTTACACTATAAGATTAGTAAGTGAAGTTTTGGAATCCAATGGGTCCACCTCCATGGGGAGTGTTTGTGCTAGTT
CCTTGTCATTGATGGATGCAGGAGTACCAATAGAAAAAGCTGTTTCAGGGATTGCCATGGGATTAATTAAAGAAGGTGAC
CAGTTGGCAATTCTAAGCGATATTCAGGGGATTGAAGACTTTCTAGGAGATATGGACTTTAAAGTAGCAGGTACTGAAGA
TGGAATTACAGCACTCCAAATGGATATCAAGATCTCTGGTACTACAAGAGAGATTTTAAAACAGGCATTGAAACAGGGTA
AAGATGGATATCTGCACATTTTAAACATAATGAAACAAACAATCAGTGAACCGAGAGAAGAATTATCTCCTCTAGCCCCC
AGGGTTATTAAAAAGCAGATTGATCCAGACAAGATTAGAAATGTCATCGGTCCTGGTGGTAAAATGATCAATAAGATCAT
TGATGAAACAGGAGTAAAAATCGACATCGAGCCCGATGGATTAATTTACATTTCTTCTTCAGATGCAGAACAAGCGGAAC
AGGCAATTAAAGCAATAGACGAATTAATTAAAGAACCTGAAGTTGGTGAAGTTTATCTCGGAAAAGTAGTTCGAACTGAA
AAGTACGGTGCTTTTGTTGAGATACTACCAGGTAAAGAAGGTTTGGTGCATATTTCCGAGCTTGCTGAAGACAGGGTCGG
GAAAACGGAGGATGTAGCCAAAGTTGGAGATGAAATTCTGGTTAAAATAATTAACATCGACGAGCGAGGACGTATAAACT
TGTCAAGAAAGCAAGCCCTCGGGGAAGAAGATGGAAAAACGAATAATGATGATAAAAAATCTACTAAAAAAACATAA

Upstream 100 bases:

>100_bases
AAAAAGTTAGGATTAAGAAAATAATCTGGTTCATACTAAGCGGGGGAGACCCCGCTTTTATTTGAATTCAAAAAAAATTA
AATAAAGGAGGGAAAAACAT

Downstream 100 bases:

>100_bases
ACCCCACGGGTTTATGTTTTTTATTTTGATCTAAAAAGAAAATCATTTTACGACATAAAAATAACTTAAACTCAGAATAG
TGATAATAATAATACATATA

Product: Polyribonucleotide nucleotidyltransferase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MPTTVSEEIAGYQLTLETGELAKQANGAVKVQYGNTVVLVTATASKEPKDDLNFFPLTVDYEERLYAVGKIPGGFIKREG
KPTEKATLTARLTDRPIRPLFPDGFRNPVHIVSTVLSVDQNCPPEIASIIGASAALSISDIPFDGPIASVKVGKVNDEIV
VTPDVDEHEESQLDLVVAGTKDAIMMVEAEANELPEDEMLEAIMKAHEEIKKIITMQEQLVEQVGQKKMEVELDLPSDEL
VSEVEELALEDIEKVLQIKDKLEREDAMDEAKQKVVDHLLEKYNSEENEDEEEELKEKHIKSAFDSILKREMRSRIIHEN
SRPDGRGQKEIRPVTCDVDLLPNTHGSGLFTRGQTQVLNVCTLGALGDVQILDGLDIEESKRYMHHYNFPPYSVGEAGFM
KGPGRREIGHGALAERALKPMIPTEKDFPYTIRLVSEVLESNGSTSMGSVCASSLSLMDAGVPIEKAVSGIAMGLIKEGD
QLAILSDIQGIEDFLGDMDFKVAGTEDGITALQMDIKISGTTREILKQALKQGKDGYLHILNIMKQTISEPREELSPLAP
RVIKKQIDPDKIRNVIGPGGKMINKIIDETGVKIDIEPDGLIYISSSDAEQAEQAIKAIDELIKEPEVGEVYLGKVVRTE
KYGAFVEILPGKEGLVHISELAEDRVGKTEDVAKVGDEILVKIINIDERGRINLSRKQALGEEDGKTNNDDKKSTKKT

Sequences:

>Translated_718_residues
MPTTVSEEIAGYQLTLETGELAKQANGAVKVQYGNTVVLVTATASKEPKDDLNFFPLTVDYEERLYAVGKIPGGFIKREG
KPTEKATLTARLTDRPIRPLFPDGFRNPVHIVSTVLSVDQNCPPEIASIIGASAALSISDIPFDGPIASVKVGKVNDEIV
VTPDVDEHEESQLDLVVAGTKDAIMMVEAEANELPEDEMLEAIMKAHEEIKKIITMQEQLVEQVGQKKMEVELDLPSDEL
VSEVEELALEDIEKVLQIKDKLEREDAMDEAKQKVVDHLLEKYNSEENEDEEEELKEKHIKSAFDSILKREMRSRIIHEN
SRPDGRGQKEIRPVTCDVDLLPNTHGSGLFTRGQTQVLNVCTLGALGDVQILDGLDIEESKRYMHHYNFPPYSVGEAGFM
KGPGRREIGHGALAERALKPMIPTEKDFPYTIRLVSEVLESNGSTSMGSVCASSLSLMDAGVPIEKAVSGIAMGLIKEGD
QLAILSDIQGIEDFLGDMDFKVAGTEDGITALQMDIKISGTTREILKQALKQGKDGYLHILNIMKQTISEPREELSPLAP
RVIKKQIDPDKIRNVIGPGGKMINKIIDETGVKIDIEPDGLIYISSSDAEQAEQAIKAIDELIKEPEVGEVYLGKVVRTE
KYGAFVEILPGKEGLVHISELAEDRVGKTEDVAKVGDEILVKIINIDERGRINLSRKQALGEEDGKTNNDDKKSTKKT
>Mature_717_residues
PTTVSEEIAGYQLTLETGELAKQANGAVKVQYGNTVVLVTATASKEPKDDLNFFPLTVDYEERLYAVGKIPGGFIKREGK
PTEKATLTARLTDRPIRPLFPDGFRNPVHIVSTVLSVDQNCPPEIASIIGASAALSISDIPFDGPIASVKVGKVNDEIVV
TPDVDEHEESQLDLVVAGTKDAIMMVEAEANELPEDEMLEAIMKAHEEIKKIITMQEQLVEQVGQKKMEVELDLPSDELV
SEVEELALEDIEKVLQIKDKLEREDAMDEAKQKVVDHLLEKYNSEENEDEEEELKEKHIKSAFDSILKREMRSRIIHENS
RPDGRGQKEIRPVTCDVDLLPNTHGSGLFTRGQTQVLNVCTLGALGDVQILDGLDIEESKRYMHHYNFPPYSVGEAGFMK
GPGRREIGHGALAERALKPMIPTEKDFPYTIRLVSEVLESNGSTSMGSVCASSLSLMDAGVPIEKAVSGIAMGLIKEGDQ
LAILSDIQGIEDFLGDMDFKVAGTEDGITALQMDIKISGTTREILKQALKQGKDGYLHILNIMKQTISEPREELSPLAPR
VIKKQIDPDKIRNVIGPGGKMINKIIDETGVKIDIEPDGLIYISSSDAEQAEQAIKAIDELIKEPEVGEVYLGKVVRTEK
YGAFVEILPGKEGLVHISELAEDRVGKTEDVAKVGDEILVKIINIDERGRINLSRKQALGEEDGKTNNDDKKSTKKT

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=723, Percent_Identity=38.5892116182573, Blast_Score=451, Evalue=1e-127,
Organism=Escherichia coli, GI145693187, Length=688, Percent_Identity=50.1453488372093, Blast_Score=677, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=727, Percent_Identity=33.1499312242091, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI281362905, Length=712, Percent_Identity=37.5, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651641, Length=712, Percent_Identity=37.5, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651643, Length=712, Percent_Identity=37.5, Blast_Score=446, Evalue=1e-125,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=36.986301369863, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_NATTJ (B2A3A3)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917621.1
- ProteinModelPortal:   B2A3A3
- GeneID:   6316894
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1450
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 79044; Mature: 78913

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTVSEEIAGYQLTLETGELAKQANGAVKVQYGNTVVLVTATASKEPKDDLNFFPLTVD
CCCCCCHHHCCEEEEEEHHHHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCEEEEEEE
YEERLYAVGKIPGGFIKREGKPTEKATLTARLTDRPIRPLFPDGFRNPVHIVSTVLSVDQ
HHHHEEEEECCCCHHHCCCCCCCCHHEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCC
NCPPEIASIIGASAALSISDIPFDGPIASVKVGKVNDEIVVTPDVDEHEESQLDLVVAGT
CCCHHHHHHHCCHHEEEECCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCEEEEEECC
KDAIMMVEAEANELPEDEMLEAIMKAHEEIKKIITMQEQLVEQVGQKKMEVELDLPSDEL
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHH
VSEVEELALEDIEKVLQIKDKLEREDAMDEAKQKVVDHLLEKYNSEENEDEEEELKEKHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KSAFDSILKREMRSRIIHENSRPDGRGQKEIRPVTCDVDLLPNTHGSGLFTRGQTQVLNV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEECCCHHHHHH
CTLGALGDVQILDGLDIEESKRYMHHYNFPPYSVGEAGFMKGPGRREIGHGALAERALKP
HHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
MIPTEKDFPYTIRLVSEVLESNGSTSMGSVCASSLSLMDAGVPIEKAVSGIAMGLIKEGD
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
QLAILSDIQGIEDFLGDMDFKVAGTEDGITALQMDIKISGTTREILKQALKQGKDGYLHI
CEEEHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCHHHH
LNIMKQTISEPREELSPLAPRVIKKQIDPDKIRNVIGPGGKMINKIIDETGVKIDIEPDG
HHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCC
LIYISSSDAEQAEQAIKAIDELIKEPEVGEVYLGKVVRTEKYGAFVEILPGKEGLVHISE
EEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEHHH
LAEDRVGKTEDVAKVGDEILVKIINIDERGRINLSRKQALGEEDGKTNNDDKKSTKKT
HHHHHCCCHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
PTTVSEEIAGYQLTLETGELAKQANGAVKVQYGNTVVLVTATASKEPKDDLNFFPLTVD
CCCCCHHHCCEEEEEEHHHHHHHCCCEEEEEECCEEEEEEECCCCCCCCCCCEEEEEEE
YEERLYAVGKIPGGFIKREGKPTEKATLTARLTDRPIRPLFPDGFRNPVHIVSTVLSVDQ
HHHHEEEEECCCCHHHCCCCCCCCHHEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCC
NCPPEIASIIGASAALSISDIPFDGPIASVKVGKVNDEIVVTPDVDEHEESQLDLVVAGT
CCCHHHHHHHCCHHEEEECCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCEEEEEECC
KDAIMMVEAEANELPEDEMLEAIMKAHEEIKKIITMQEQLVEQVGQKKMEVELDLPSDEL
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHH
VSEVEELALEDIEKVLQIKDKLEREDAMDEAKQKVVDHLLEKYNSEENEDEEEELKEKHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KSAFDSILKREMRSRIIHENSRPDGRGQKEIRPVTCDVDLLPNTHGSGLFTRGQTQVLNV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEECCCHHHHHH
CTLGALGDVQILDGLDIEESKRYMHHYNFPPYSVGEAGFMKGPGRREIGHGALAERALKP
HHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
MIPTEKDFPYTIRLVSEVLESNGSTSMGSVCASSLSLMDAGVPIEKAVSGIAMGLIKEGD
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
QLAILSDIQGIEDFLGDMDFKVAGTEDGITALQMDIKISGTTREILKQALKQGKDGYLHI
CEEEHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEECCCHHHHHHHHHHCCCCCHHHH
LNIMKQTISEPREELSPLAPRVIKKQIDPDKIRNVIGPGGKMINKIIDETGVKIDIEPDG
HHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCC
LIYISSSDAEQAEQAIKAIDELIKEPEVGEVYLGKVVRTEKYGAFVEILPGKEGLVHISE
EEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEHHH
LAEDRVGKTEDVAKVGDEILVKIINIDERGRINLSRKQALGEEDGKTNNDDKKSTKKT
HHHHHCCCHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA