Click here to switch to the map view.

The map label for this gene is hslU

Identifier: 188586011

GI number: 188586011

Start: 1456983

End: 1458329

Strand: Direct

Name: hslU

Synonym: Nther_1385

Alternate gene names: 188586011

Gene position: 1456983-1458329 (Clockwise)

Preceding gene: 188586010

Following gene: 188586012

Centisome position: 46.03

GC content: 35.26

Gene sequence:

>1347_bases
ATGATTATTTCAACAGAAGAACTGACACCCCAAGAAATAGTAGAGCGATTAGATAAATATATTATTGGTCAAAACAAAGC
AAAGAGATCTATGGCTATAGCAATTAGGAATAGGTATCGCAGACAAAAACTTGAAGAAGAACTTAAAGAAGAAGTTATTC
CTAAAAATATATTGATGATTGGCCCTACAGGAGTTGGGAAAACTGAAATAGCTAGGAGACTGGCAAAACTTATCAAGGCA
CCTTTTATAAAAATTGAAGTTACTAAGTTTACAGAAGTAGGTTATGTAGGTAGAAACGTTGAATCTATGGTCAAAGATTT
AGTAGCTGAATCCATTCAGTTAGTAGAAGAGGAAAAGCATGAAGAAGTTTCTAAACAAGCACGTAAGCAAGCTGAAAATA
GAATTATAGATTTATTATTGTCAGAGAAAAAATCTTCTTCCAAAGGTGAGCAAGAAACTGATCTGAGAACAAGAAGACGG
GAAATTAAAGAACAATTGTTTAAAGGCTTATTGGAAGATGAGTATGTAACAGTTAAACTAGAAAAAGAACCGGCTCAAGA
TGATAACTTGATGGGAGAACAGTTTGAAGAAATGGGAGTTAATTTTAAAGAATTGTTTGGAAAAATATTACCTAAAGAAT
CTGAACACAAGAAAATGACTGTGAAAGAAGCTAGAAAAGTTTTAACTCGACAAGAAGCCGATAAATTAATTAATAAGGAT
CAGGTAAATCAAGAAGCTATTGAAAGAGCAGAAAATTATGGTATCATTTTCTTGGATGAGTTTGATAAAATAGCAGCATC
TTCAGGAAAACAATCCGGTGGTCAAGATGTTTCACGTGAAGGGGTACAAAGGGACATACTTCCTATTGTAGAAGGTTCGA
CAGTGAACACTAGATACGGTCGAGTCAAGACTGATCACATTATGTTTATAGGAGCTGGAGCCTTTCACGATAGTAAACCG
GCGGACTTAATACCTGAATTGCAGGGTAGATTCCCCATCAGAGTTGAACTGGATAGCCTGACCAAGGATGACTTTTATCG
GATTCTGACAGAGCCTAGCAACGCTTTGCTAGAACAATATAAGGCCTTGTTAAGAACTGAAGAAGTAAAGGTCAATTTCA
CAGAAGATGCCATCGAAGAACTTGCCAATATGTGTGAGTACGTAAATGAAAATACTGAAGATATTGGGGCTAGAAGACTG
CATACAATTTTAGAAACATTACTGGAAGAATTATCATTTTATGCTCCAGAAATGAAAGGTCAAGAGGTTGAGATCAACTC
CAATTATGTTAAAGATCAACTTCAAGATATTGTGACTGAAAAAGATAGCAGTCGGTTTATCTTGTAA

Upstream 100 bases:

>100_bases
TGTGTACACTAATTCCCATATTTCCGTGGAAGAGTTGAGAGCACAGGGAGACTAATTGACAGTTCAATTAGTATAAAGAA
TTGACCGTAAAAGGCAGGTG

Downstream 100 bases:

>100_bases
ATGACTTAACAGTTTATTGATTGAAGGTGGGATTTTTTCATGGAAATGAAAAGTATTAGTGACTTTTCTATTATACTTAC
TAGATAAAACAAAAAATTCT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MIISTEELTPQEIVERLDKYIIGQNKAKRSMAIAIRNRYRRQKLEEELKEEVIPKNILMIGPTGVGKTEIARRLAKLIKA
PFIKIEVTKFTEVGYVGRNVESMVKDLVAESIQLVEEEKHEEVSKQARKQAENRIIDLLLSEKKSSSKGEQETDLRTRRR
EIKEQLFKGLLEDEYVTVKLEKEPAQDDNLMGEQFEEMGVNFKELFGKILPKESEHKKMTVKEARKVLTRQEADKLINKD
QVNQEAIERAENYGIIFLDEFDKIAASSGKQSGGQDVSREGVQRDILPIVEGSTVNTRYGRVKTDHIMFIGAGAFHDSKP
ADLIPELQGRFPIRVELDSLTKDDFYRILTEPSNALLEQYKALLRTEEVKVNFTEDAIEELANMCEYVNENTEDIGARRL
HTILETLLEELSFYAPEMKGQEVEINSNYVKDQLQDIVTEKDSSRFIL

Sequences:

>Translated_448_residues
MIISTEELTPQEIVERLDKYIIGQNKAKRSMAIAIRNRYRRQKLEEELKEEVIPKNILMIGPTGVGKTEIARRLAKLIKA
PFIKIEVTKFTEVGYVGRNVESMVKDLVAESIQLVEEEKHEEVSKQARKQAENRIIDLLLSEKKSSSKGEQETDLRTRRR
EIKEQLFKGLLEDEYVTVKLEKEPAQDDNLMGEQFEEMGVNFKELFGKILPKESEHKKMTVKEARKVLTRQEADKLINKD
QVNQEAIERAENYGIIFLDEFDKIAASSGKQSGGQDVSREGVQRDILPIVEGSTVNTRYGRVKTDHIMFIGAGAFHDSKP
ADLIPELQGRFPIRVELDSLTKDDFYRILTEPSNALLEQYKALLRTEEVKVNFTEDAIEELANMCEYVNENTEDIGARRL
HTILETLLEELSFYAPEMKGQEVEINSNYVKDQLQDIVTEKDSSRFIL
>Mature_448_residues
MIISTEELTPQEIVERLDKYIIGQNKAKRSMAIAIRNRYRRQKLEEELKEEVIPKNILMIGPTGVGKTEIARRLAKLIKA
PFIKIEVTKFTEVGYVGRNVESMVKDLVAESIQLVEEEKHEEVSKQARKQAENRIIDLLLSEKKSSSKGEQETDLRTRRR
EIKEQLFKGLLEDEYVTVKLEKEPAQDDNLMGEQFEEMGVNFKELFGKILPKESEHKKMTVKEARKVLTRQEADKLINKD
QVNQEAIERAENYGIIFLDEFDKIAASSGKQSGGQDVSREGVQRDILPIVEGSTVNTRYGRVKTDHIMFIGAGAFHDSKP
ADLIPELQGRFPIRVELDSLTKDDFYRILTEPSNALLEQYKALLRTEEVKVNFTEDAIEELANMCEYVNENTEDIGARRL
HTILETLLEELSFYAPEMKGQEVEINSNYVKDQLQDIVTEKDSSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Homo sapiens, GI7242140, Length=108, Percent_Identity=38.8888888888889, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790366, Length=443, Percent_Identity=50.5643340857788, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1786642, Length=101, Percent_Identity=44.5544554455446, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71982908, Length=239, Percent_Identity=31.7991631799163, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71982905, Length=239, Percent_Identity=31.7991631799163, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319704, Length=472, Percent_Identity=23.728813559322, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648291, Length=250, Percent_Identity=32.4, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648289, Length=250, Percent_Identity=32.4, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_NATTJ (B2A337)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917556.1
- ProteinModelPortal:   B2A337
- SMR:   B2A337
- GeneID:   6316638
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1385
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 51514; Mature: 51514

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: BINDING 22-22 BINDING 259-259 BINDING 326-326 BINDING 398-398

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIISTEELTPQEIVERLDKYIIGQNKAKRSMAIAIRNRYRRQKLEEELKEEVIPKNILMI
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GPTGVGKTEIARRLAKLIKAPFIKIEVTKFTEVGYVGRNVESMVKDLVAESIQLVEEEKH
CCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EEVSKQARKQAENRIIDLLLSEKKSSSKGEQETDLRTRRREIKEQLFKGLLEDEYVTVKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
EKEPAQDDNLMGEQFEEMGVNFKELFGKILPKESEHKKMTVKEARKVLTRQEADKLINKD
ECCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHH
QVNQEAIERAENYGIIFLDEFDKIAASSGKQSGGQDVSREGVQRDILPIVEGSTVNTRYG
CCCHHHHHHHHCCCEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
RVKTDHIMFIGAGAFHDSKPADLIPELQGRFPIRVELDSLTKDDFYRILTEPSNALLEQY
CEEECEEEEEEECCCCCCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHH
KALLRTEEVKVNFTEDAIEELANMCEYVNENTEDIGARRLHTILETLLEELSFYAPEMKG
HHHHHHHHHEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QEVEINSNYVKDQLQDIVTEKDSSRFIL
CEEEECHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIISTEELTPQEIVERLDKYIIGQNKAKRSMAIAIRNRYRRQKLEEELKEEVIPKNILMI
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GPTGVGKTEIARRLAKLIKAPFIKIEVTKFTEVGYVGRNVESMVKDLVAESIQLVEEEKH
CCCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
EEVSKQARKQAENRIIDLLLSEKKSSSKGEQETDLRTRRREIKEQLFKGLLEDEYVTVKL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
EKEPAQDDNLMGEQFEEMGVNFKELFGKILPKESEHKKMTVKEARKVLTRQEADKLINKD
ECCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHH
QVNQEAIERAENYGIIFLDEFDKIAASSGKQSGGQDVSREGVQRDILPIVEGSTVNTRYG
CCCHHHHHHHHCCCEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
RVKTDHIMFIGAGAFHDSKPADLIPELQGRFPIRVELDSLTKDDFYRILTEPSNALLEQY
CEEECEEEEEEECCCCCCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCHHHHHHHH
KALLRTEEVKVNFTEDAIEELANMCEYVNENTEDIGARRLHTILETLLEELSFYAPEMKG
HHHHHHHHHEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QEVEINSNYVKDQLQDIVTEKDSSRFIL
CEEEECHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA