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The map label for this gene is dapE [H]

Identifier: 188585620

GI number: 188585620

Start: 1057557

End: 1058915

Strand: Reverse

Name: dapE [H]

Synonym: Nther_0993

Alternate gene names: 188585620

Gene position: 1058915-1057557 (Counterclockwise)

Preceding gene: 188585621

Following gene: 188585615

Centisome position: 33.45

GC content: 36.79

Gene sequence:

>1359_bases
ATGATTTCTCAAGTTTTTTCCCATATAGAGGATAATAAAGAGAAGTATTTAGATGAGTTAAAAAAGTTGGTAAAACAACC
AAGTATCAGTACTAAAAACCAAGGAATAACTGAATGCGCAAAAATGATACAAAAATTGATGAAAGAAACTGGAATCACAA
CTAAACTAATCCCTACTTCAGGTCATCCCATAATCTATGGCGAGTTAATCACTTCAGAATCAGCACCCACAATAATCTTT
TACGGTCATTACGACGTACAACCCCCTGAACCATATGAATTATGGCATACCGAACCTTTTGAACCAGAGGTGAAAAACCA
ACGTCTTTATGGACGAGGAGTAGGAGACAACAAGGGACAGTTATTAACCCATATTTTAGCAGTTCGGTCTTATTTGGAAA
CAAAAGGTCAACTTCCAGTTAATGTAAAGTTCGTTTTCGAAGGAGAAGAAGAAATAGGCAGTCCACATATAGCTCAATTT
GTAAAAGAAAATAAAAATTTGCTTAAGGGTGACTTAGTTTATATTTCAGACGGGCCACTGGCACCTGGAGACGCGCCAGT
GGTTTCTCTTGGAAATCGGGGGATATTATCAGTCCAATTAAGTATCAAAACTGCAAATAGAGATAATCATTCTGGTAATC
GTGGTGGGGTTATTCCAAATGCCGCTTGGGAACTAGTAACCGTGCTAAACTCCCTTAAAAATGAAAAAAATAAAGTATTG
ATCCCTGAATTCTATAAAGATGTCATAAAACCTGGATCAGCAGAACTCGACTTGATTCAACGATTGCCATTTGACTCGCA
AAAAACAGCCGAAGCTTTTTATGTAGATGAAAGCGATTTAGAGAAAGAAAATTTTTATAAAAAACTAACACTAGAACCAG
TATTAAATATCAATGGTATCGTAAGCGGTTATACCGGTGAAGGAACTAAAACCATTATCCCCTGTCAAGCTAGTGCTAAA
CTTGATATGAGATTAGTACCAGACCAGGATCCAGAACATATTTTCCATAGACTTTCAGAATGGGTCAAAAAAATAAATCC
CCGCACCCAAGTTGAGATGCAGGGACGTTACATGTATCCGTCCAGAACAAGTCCTAAACTTTCAGCTATTCAACATATAA
CAAAATCTGTTGAACAAGTTCATAATACAAAACCATTAGTCTATCCTTCCACAGGTGGCAGTTTACCCACCTATGTTTGG
ACGAAAATTTTAAATTTACCAGCTGTCACTGTTCCCTATGCCAATTCAGACGAAGCCAATCATGCTCCTAATGAAAATTT
GAAGCTAGATTTGTTCTATAAAGGAATAAAAACATCTTGCCAGGTCCTTGATAATTTATCTCTGCTCAAAAATCACTGA

Upstream 100 bases:

>100_bases
GTGCCTTGGCCTTTTGTCAGGGAATTGAAAAAGGAGTTAACTAGTGTGCTTTAACTAGTGTACTTTTTGGTTACATCCAA
ACTAAAAAGGGAGTGTCAAA

Downstream 100 bases:

>100_bases
TAATTAAAGCCTATAATAATCATTCTCAACGATAAACCAAACTCAACGATGAATCAAAAACCATAGTCTTAAAGATACTA
ATACTAAAGATTTAAAGATA

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MISQVFSHIEDNKEKYLDELKKLVKQPSISTKNQGITECAKMIQKLMKETGITTKLIPTSGHPIIYGELITSESAPTIIF
YGHYDVQPPEPYELWHTEPFEPEVKNQRLYGRGVGDNKGQLLTHILAVRSYLETKGQLPVNVKFVFEGEEEIGSPHIAQF
VKENKNLLKGDLVYISDGPLAPGDAPVVSLGNRGILSVQLSIKTANRDNHSGNRGGVIPNAAWELVTVLNSLKNEKNKVL
IPEFYKDVIKPGSAELDLIQRLPFDSQKTAEAFYVDESDLEKENFYKKLTLEPVLNINGIVSGYTGEGTKTIIPCQASAK
LDMRLVPDQDPEHIFHRLSEWVKKINPRTQVEMQGRYMYPSRTSPKLSAIQHITKSVEQVHNTKPLVYPSTGGSLPTYVW
TKILNLPAVTVPYANSDEANHAPNENLKLDLFYKGIKTSCQVLDNLSLLKNH

Sequences:

>Translated_452_residues
MISQVFSHIEDNKEKYLDELKKLVKQPSISTKNQGITECAKMIQKLMKETGITTKLIPTSGHPIIYGELITSESAPTIIF
YGHYDVQPPEPYELWHTEPFEPEVKNQRLYGRGVGDNKGQLLTHILAVRSYLETKGQLPVNVKFVFEGEEEIGSPHIAQF
VKENKNLLKGDLVYISDGPLAPGDAPVVSLGNRGILSVQLSIKTANRDNHSGNRGGVIPNAAWELVTVLNSLKNEKNKVL
IPEFYKDVIKPGSAELDLIQRLPFDSQKTAEAFYVDESDLEKENFYKKLTLEPVLNINGIVSGYTGEGTKTIIPCQASAK
LDMRLVPDQDPEHIFHRLSEWVKKINPRTQVEMQGRYMYPSRTSPKLSAIQHITKSVEQVHNTKPLVYPSTGGSLPTYVW
TKILNLPAVTVPYANSDEANHAPNENLKLDLFYKGIKTSCQVLDNLSLLKNH
>Mature_452_residues
MISQVFSHIEDNKEKYLDELKKLVKQPSISTKNQGITECAKMIQKLMKETGITTKLIPTSGHPIIYGELITSESAPTIIF
YGHYDVQPPEPYELWHTEPFEPEVKNQRLYGRGVGDNKGQLLTHILAVRSYLETKGQLPVNVKFVFEGEEEIGSPHIAQF
VKENKNLLKGDLVYISDGPLAPGDAPVVSLGNRGILSVQLSIKTANRDNHSGNRGGVIPNAAWELVTVLNSLKNEKNKVL
IPEFYKDVIKPGSAELDLIQRLPFDSQKTAEAFYVDESDLEKENFYKKLTLEPVLNINGIVSGYTGEGTKTIIPCQASAK
LDMRLVPDQDPEHIFHRLSEWVKKINPRTQVEMQGRYMYPSRTSPKLSAIQHITKSVEQVHNTKPLVYPSTGGSLPTYVW
TKILNLPAVTVPYANSDEANHAPNENLKLDLFYKGIKTSCQVLDNLSLLKNH

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=475, Percent_Identity=26.7368421052632, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI21071039, Length=467, Percent_Identity=25.0535331905782, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI271398277, Length=326, Percent_Identity=26.0736196319018, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1788816, Length=186, Percent_Identity=25.8064516129032, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193211059, Length=471, Percent_Identity=28.4501061571125, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17563146, Length=462, Percent_Identity=28.1385281385281, Blast_Score=174, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6321159, Length=472, Percent_Identity=28.8135593220339, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6319758, Length=444, Percent_Identity=27.2522522522523, Blast_Score=163, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24585880, Length=460, Percent_Identity=27.3913043478261, Blast_Score=178, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50668; Mature: 50668

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISQVFSHIEDNKEKYLDELKKLVKQPSISTKNQGITECAKMIQKLMKETGITTKLIPTS
CHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCC
GHPIIYGELITSESAPTIIFYGHYDVQPPEPYELWHTEPFEPEVKNQRLYGRGVGDNKGQ
CCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHH
LLTHILAVRSYLETKGQLPVNVKFVFEGEEEIGSPHIAQFVKENKNLLKGDLVYISDGPL
HHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHCCHHHHHHHHHCCCEEECCEEEEECCCC
APGDAPVVSLGNRGILSVQLSIKTANRDNHSGNRGGVIPNAAWELVTVLNSLKNEKNKVL
CCCCCCEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
IPEFYKDVIKPGSAELDLIQRLPFDSQKTAEAFYVDESDLEKENFYKKLTLEPVLNINGI
CHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECHHCCCHHHHHHHEECCCCCCCCCE
VSGYTGEGTKTIIPCQASAKLDMRLVPDQDPEHIFHRLSEWVKKINPRTQVEMQGRYMYP
EECCCCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCEEECC
SRTSPKLSAIQHITKSVEQVHNTKPLVYPSTGGSLPTYVWTKILNLPAVTVPYANSDEAN
CCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCC
HAPNENLKLDLFYKGIKTSCQVLDNLSLLKNH
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISQVFSHIEDNKEKYLDELKKLVKQPSISTKNQGITECAKMIQKLMKETGITTKLIPTS
CHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCC
GHPIIYGELITSESAPTIIFYGHYDVQPPEPYELWHTEPFEPEVKNQRLYGRGVGDNKGQ
CCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHH
LLTHILAVRSYLETKGQLPVNVKFVFEGEEEIGSPHIAQFVKENKNLLKGDLVYISDGPL
HHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHCCHHHHHHHHHCCCEEECCEEEEECCCC
APGDAPVVSLGNRGILSVQLSIKTANRDNHSGNRGGVIPNAAWELVTVLNSLKNEKNKVL
CCCCCCEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
IPEFYKDVIKPGSAELDLIQRLPFDSQKTAEAFYVDESDLEKENFYKKLTLEPVLNINGI
CHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEECHHCCCHHHHHHHEECCCCCCCCCE
VSGYTGEGTKTIIPCQASAKLDMRLVPDQDPEHIFHRLSEWVKKINPRTQVEMQGRYMYP
EECCCCCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEECCEEECC
SRTSPKLSAIQHITKSVEQVHNTKPLVYPSTGGSLPTYVWTKILNLPAVTVPYANSDEAN
CCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCC
HAPNENLKLDLFYKGIKTSCQVLDNLSLLKNH
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA