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The map label for this gene is exoA [H]

Identifier: 188585577

GI number: 188585577

Start: 1005162

End: 1005917

Strand: Direct

Name: exoA [H]

Synonym: Nther_0949

Alternate gene names: 188585577

Gene position: 1005162-1005917 (Clockwise)

Preceding gene: 188585575

Following gene: 188585578

Centisome position: 31.75

GC content: 34.92

Gene sequence:

>756_bases
ATGAAATTGATTTCATGGAACGTAAATGGTTTAAGAGCATGTCTCAAGAAAGGATTTTTAGAGTACTTTAATGAGGTTTC
CGGAGACATTTTTTGTGTTCAGGAAACAAAGTTACAAGAAGGTCAGATAGACTTAGAGTTACCTGGGTATTTTCAGTACT
GGAATTATGCTGAGAAAAAAGGGTATTCAGGAACAGCTATTTTCACAAAAATCCAACCATTAACTGTCCAGTATGGAATA
GGTGAAGCTAAGCATGATAAAGAAGGAAGAGTTATTACTTTAGAATTTTGTGATTACTATTTGGTGAATGTGTATACTCC
TAACTCTCAAAGAGAACTAGCCAGGTTGGATTATAGATTACAGTGGGAAGATGCTTTTAAGAATTACTTAGTCAATTTAG
ATTCTGAAAAACCTGTGATTGTATGTGGTGATCTAAATGTAGCACATAAAGAAATTGATTTGAAAAATCCCAAAAATAAT
AAAAGAAATGCTGGGTTCACAGAAGAAGAACGGAATAAGTTGACTGAACTTTTAAATGCGGGCTTTATTGATACATTTAG
ATACTTCTATCCCGATACAGAAGGTGCATATACCTGGTGGTCGTACATTACAAAAGCAAGAGAAAGAAATGCTGGCTGGA
GAATTGATTACTTTTTAGCCTCTCAGAAGTTAGATAACTGGTTAACTGATGCAGAAATCCATGCAGATATAATGGGAAGT
GACCATTGCCCTGTGGCATTAAAATTGGCGTCTTAA

Upstream 100 bases:

>100_bases
AGATAGTTCTAACTTGATTTTTGAATATTATATTCCAATATTTTTACTTTTATTGAATGTTATAATTAAATTAAAAGTGG
AGACAGGGGTGAACTGGTAA

Downstream 100 bases:

>100_bases
ATTTAAAATAACAGTAAGCTTTAAAATGGCTGTATTTACCACGTAAAATTAAAATACTTTTTGTATCCCCCTTCGTCTAT
TATAAAAGCAAAGAGAAAAG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKLISWNVNGLRACLKKGFLEYFNEVSGDIFCVQETKLQEGQIDLELPGYFQYWNYAEKKGYSGTAIFTKIQPLTVQYGI
GEAKHDKEGRVITLEFCDYYLVNVYTPNSQRELARLDYRLQWEDAFKNYLVNLDSEKPVIVCGDLNVAHKEIDLKNPKNN
KRNAGFTEEERNKLTELLNAGFIDTFRYFYPDTEGAYTWWSYITKARERNAGWRIDYFLASQKLDNWLTDAEIHADIMGS
DHCPVALKLAS

Sequences:

>Translated_251_residues
MKLISWNVNGLRACLKKGFLEYFNEVSGDIFCVQETKLQEGQIDLELPGYFQYWNYAEKKGYSGTAIFTKIQPLTVQYGI
GEAKHDKEGRVITLEFCDYYLVNVYTPNSQRELARLDYRLQWEDAFKNYLVNLDSEKPVIVCGDLNVAHKEIDLKNPKNN
KRNAGFTEEERNKLTELLNAGFIDTFRYFYPDTEGAYTWWSYITKARERNAGWRIDYFLASQKLDNWLTDAEIHADIMGS
DHCPVALKLAS
>Mature_251_residues
MKLISWNVNGLRACLKKGFLEYFNEVSGDIFCVQETKLQEGQIDLELPGYFQYWNYAEKKGYSGTAIFTKIQPLTVQYGI
GEAKHDKEGRVITLEFCDYYLVNVYTPNSQRELARLDYRLQWEDAFKNYLVNLDSEKPVIVCGDLNVAHKEIDLKNPKNN
KRNAGFTEEERNKLTELLNAGFIDTFRYFYPDTEGAYTWWSYITKARERNAGWRIDYFLASQKLDNWLTDAEIHADIMGS
DHCPVALKLAS

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=256, Percent_Identity=51.171875, Blast_Score=272, Evalue=2e-73,
Organism=Homo sapiens, GI18375503, Length=256, Percent_Identity=51.171875, Blast_Score=272, Evalue=2e-73,
Organism=Homo sapiens, GI18375501, Length=256, Percent_Identity=51.171875, Blast_Score=272, Evalue=2e-73,
Organism=Homo sapiens, GI18375507, Length=314, Percent_Identity=28.0254777070064, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788046, Length=264, Percent_Identity=31.0606060606061, Blast_Score=114, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71989536, Length=254, Percent_Identity=40.9448818897638, Blast_Score=207, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI71989539, Length=144, Percent_Identity=36.1111111111111, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17136678, Length=250, Percent_Identity=52, Blast_Score=285, Evalue=2e-77,
Organism=Drosophila melanogaster, GI221330655, Length=250, Percent_Identity=52, Blast_Score=285, Evalue=2e-77,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29197; Mature: 29197

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNVNGLRACLKKGFLEYFNEVSGDIFCVQETKLQEGQIDLELPGYFQYWNYAEKK
CEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCHHHHCCHHHHC
GYSGTAIFTKIQPLTVQYGIGEAKHDKEGRVITLEFCDYYLVNVYTPNSQRELARLDYRL
CCCCCEEEEEEEEEEEEECCCCCCCCCCCCEEEEEECCEEEEEEECCCCHHHHHHHCEEE
QWEDAFKNYLVNLDSEKPVIVCGDLNVAHKEIDLKNPKNNKRNAGFTEEERNKLTELLNA
EHHHHHHHHEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
GFIDTFRYFYPDTEGAYTWWSYITKARERNAGWRIDYFLASQKLDNWLTDAEIHADIMGS
CCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCEEEEECCC
DHCPVALKLAS
CCCCEEEEECC
>Mature Secondary Structure
MKLISWNVNGLRACLKKGFLEYFNEVSGDIFCVQETKLQEGQIDLELPGYFQYWNYAEKK
CEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCHHHHCCHHHHC
GYSGTAIFTKIQPLTVQYGIGEAKHDKEGRVITLEFCDYYLVNVYTPNSQRELARLDYRL
CCCCCEEEEEEEEEEEEECCCCCCCCCCCCEEEEEECCEEEEEEECCCCHHHHHHHCEEE
QWEDAFKNYLVNLDSEKPVIVCGDLNVAHKEIDLKNPKNNKRNAGFTEEERNKLTELLNA
EHHHHHHHHEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
GFIDTFRYFYPDTEGAYTWWSYITKARERNAGWRIDYFLASQKLDNWLTDAEIHADIMGS
CCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHCCCCCEEEEECCC
DHCPVALKLAS
CCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]